General Process-Based Model for Describing the Metabolic Shift in Microbial Cell Cultures

November 12, 2020
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eaaci2015

Cell Cultures for Virology: Usability, Advantages, and Prospects

Virus detection in natural and clinical samples is a complicated problem in research and diagnostics. There are different approaches for virus isolation and identification, including PCR, CRISPR/Cas technology, NGS, immunoassays, and cell-based assays. Following the development of genetic engineering methods, approaches that utilize cell cultures have become useful and informative. Molecular biology methods allow increases in the sensitivity and specificity of cell cultures for certain viruses and can be used to generate reporter cell lines.
These cell lines express specific reporter proteins (e.g., GFP, luciferase, and CAT) in response to virus infection that can be detected in a laboratory setting. The development of genome editing and synthetic biology methods has given rise to new perspectives regarding the design of virus reporter systems in cell cultures. This review is aimed at describing both virology methods in general and examples of the development of cell-based methods that exist today.

A General Process-Based Model for Describing the Metabolic Shift in Microbial Cell Cultures

The metabolic shift between respiration and fermentation at high glucose concentration is a widespread phenomenon in microbial world, and it is relevant for the biotechnological exploitation of microbial cell factories, affecting the achievement of high-cell-densities in bioreactors. Starting from a model already developed for the yeast Saccharomyces cerevisiae, based on the System Dynamics approach, a general process-based model for two prokaryotic species of biotechnological interest, such as Escherichia coli and Bacillus subtilis, is proposed. The model is based on the main assumption that glycolytic intermediates act as central catabolic hub regulating the shift between respiratory and fermentative pathways. Furthermore, the description of a mixed fermentation with secondary by-products, characteristic of bacterial metabolism, is explicitly considered.
The model also represents the inhibitory effect on growth and metabolism of self-produced toxic compounds relevant in assessing the late phases of high-cell density culture. Model simulations reproduced data from experiments reported in the literature with different strains of non-recombinant and recombinant E. coli and B. subtilis cultured in both batch and fed-batch reactors. The proposed model, based on simple biological assumptions, is able to describe the main dynamics of two microbial species of relevant biotechnological interest. It demonstrates that a reductionist System Dynamics approach to formulate simplified macro-kinetic models can provide a robust representation of cell growth and accumulation in the medium of fermentation by-products.

Developmentally-Inspired Biomimetic Culture Models to Produce Functional Islet-Like Cells From Pluripotent Precursors

Insulin-producing beta cells sourced from pluripotent stem cells hold great potential as a virtually unlimited cell source to treat diabetes. Directed pancreatic differentiation protocols aim to mimic various stimuli present during embryonic development through sequential changes of in vitro culture conditions. This is commonly accomplished by the timed addition of soluble signaling factors, in conjunction with cell-handling steps such as the formation of 3D cell aggregates. Interestingly, when stem cells at the pancreatic progenitor stage are transplanted, they form functional insulin-producing cells, suggesting that in vivo microenvironmental cues promote beta cell specification. Among these cues, biophysical stimuli have only recently emerged in the context of optimizing pancreatic differentiation protocols.
This review focuses on studies of cell-microenvironment interactions and their impact on differentiating pancreatic cells when considering cell signaling, cell-cell and cell-ECM interactions. We highlight the development of in vitro cell culture models that allow systematic studies of pancreatic cell mechanobiology in response to extracellular matrix proteins, biomechanical effects, soluble factor modulation of biomechanics, substrate stiffness, fluid flow and topography. Finally, we explore how these new mechanical insights could lead to novel pancreatic differentiation protocols that improve efficiency, maturity, and throughput.

Effects of FGFR inhibitors TKI258, BGJ398 and AZD4547 on breast cancer cells in 2D, 3D and tissue explant cultures

Purpose: Fibroblast growth factor receptors (FGFR) and pathways are important players in breast cancer (BC) development. They are commonly altered, and BCs exhibiting FGFR gene amplification are currently being studied for drug development. Here, we aimed to compare the effects of three FGFR inhibitors (FGFRis), i.e., non-selective TKI258 and selective BGJ398 and AZD4547, on different BC-derived cell lines (BCCs) and primary tissues.
Methods: The human BCCs MCF-7 and MDA-MB-231(SA) (wild-type FGFR) and MFM223 (amplified FGFR1 and FGFR2) were analyzed for FGFR expression using qRT-PCR, and the effects of FGFRis on FGFR signaling by Western blotting. The effects of FGFRis on proliferation, viability, migration and invasion of BCCs were assessed in 2D cultures using live-cell imaging, and in 3D cultures using phenotypic analysis of organoids. To study radio-sensitization, FGFRi treatment was combined with irradiation. Patient-derived BC samples were treated with FGFRis in explant cultures and immunostained for Ki67 and cleaved caspase 3.
Results: We found that all FGFRis tested decreased the growth and viability of BC cells in 2D and 3D cultures. BGJ398 and AZD4547 were found to be potent at low concentrations in FGFR-amplified MFM233 cells, whereas higher concentrations were required in non-amplified MCF7 and MDA-MB-231(SA) cells. TKI258 inhibited the migration and invasion, whereas BGJ398 and AZD4547 only inhibited the invasion of MDA-MB-231(SA) cells. FGFRi treatment of MCF7 and MFM223 cells enhanced the inhibitory effect of radiotherapy, but this effect was not observed in MDA-MB-231(SA) cells. FGFRi-treated primary BC explants with moderate FGFR levels showed a tendency towards decreased proliferation and increased apoptosis.
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Peripheral Blood Leukocyte (PBL's) Lysate
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Description: Peripheral Blood Leukocyte (PBL's) tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

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ASE-9234 1 vial (1 x 10^6)
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cDNA from Human Adult Normal Tissue: Peripheral Blood Leukocyte
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Beagle dog Red Blood Cells (100%)
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IMEMS Random Nanofibers 6 Chambers
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Methylated & Low-Methylated DNA Matching Pair - Human Peripheral Blood Leukocyte
D6234148-PP 2x5 ug
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Total Protein from Human Adult Normal Tissue: Peripheral Blood Leukocyte
P1234148 1 mg
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein from Human Adult Normal Tissue: Peripheral Blood Leukocyte
P3234148 0.1 mg
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Human Adult Normal Tissue: Peripheral Blood Leukocyte
R1234148-10 10 ug
EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Rat Anti Rat Rt6.1 (Peripheral T Cells) Monoclonal Antibody
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Human  Peripheral  Blood  CD14+ Monocytes, Cryopreserved / Untouched
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EUR 118

Genomic DNA from Human Adult Normal Tissue: Peripheral Blood Leukocyte, from a single donor
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EUR 282
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Recombinant Peripheral Myelin Protein 22 (PMP22)
4-RPC746Hu01
  • EUR 449.44
  • EUR 223.00
  • EUR 1410.40
  • EUR 536.80
  • EUR 973.60
  • EUR 364.00
  • EUR 3376.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Peripheral Myelin Protein 22 expressed in: E.coli

Rat Peripheral myelin protein 22 (Pmp22)
1-CSB-CF018241RA
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  • EUR 482.00
  • EUR 738.00
  • 1MG
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Description: Recombinant Rat Peripheral myelin protein 22(Pmp22) expressed in in vitro E.coli expression system

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20-abx114394
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  • EUR 398.00
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20-abx304860
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  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
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Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx103057
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  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
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  • 100 ug
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  • 1 mg
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Recombinant Human Peripheral Myelin Protein-2
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Recombinant Human Peripheral Myelin Protein-2
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Recombinant Human Peripheral Myelin Protein-2
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Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx131854
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  • EUR 857.00
  • EUR 439.00
  • EUR 154.00
  • EUR 258.00
  • 100 ug
  • 1 mg
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  • 20 ug
  • 50 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx133606
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  • EUR 439.00
  • EUR 189.00
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Peripheral Myelin Protein 22 (PMP22) Antibody
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Peripheral Myelin Protein 22 (PMP22) Antibody
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EUR 286

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx177954
  • EUR 1233.00
  • EUR 592.00
  • 1 mg
  • 200 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx177955
  • EUR 1233.00
  • EUR 592.00
  • 1 mg
  • 200 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx177956
  • EUR 453.00
  • EUR 133.00
  • EUR 1302.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx173998
  • EUR 913.00
  • EUR 467.00
  • 1 mg
  • 200 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx217831
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx328779
  • EUR 314.00
  • EUR 244.00
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Recombinant mouse Peripheral myelin protein 22
P1724 100ug Ask for price
Description: Recombinant protein for mouse Peripheral myelin protein 22

Recombinant rat Peripheral myelin protein 22
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Description: Recombinant protein for rat Peripheral myelin protein 22
Conclusions: Our results indicate that, besides targeting FGFR-amplified BCs with selective FGFRis, also BCs without FGFR amplification/activation may benefit from FGFRi-treatment. Combination with other treatment modalities, such as radiotherapy, may allow the use of FGFRis at relatively low concentrations and, thereby, contribute to better BC treatment outcomes.

Inflammatory lncRNA AK039862 regulates paraquat-inhibited proliferation and migration of microglial and neuronal cells through the Pafah1b1/Foxa1 pathway in co-culture environments

 

Emerging evidences having suggested that particular lncRNAs have a potential effect on PD progression through provoking damage and inflammatory responses of microglia/ dopaminergic cells. In addition, paraquat can be accumulated in human body through various approaches and have an increased risk for Parkinson’s disease. However, the specific role and mechanism of lncRNA related to neurotoxic in the progression of PD is unclear. In our study, a mouse PD model was established induced by the intraperitoneal injection of paraquat (5 mg/kg and 10 mg/kg) every three days (10 times).
We determined differential expression of lncRNA AK039862 and its potential targeted genes Pafah1b1/Foxa1 in PD mouse model, then we used fluorescence in situ hybridization (FISH) to visualize the cellular distribution of AK039862. Short interfering RNAs (siRNAs) and overexpression plasmids were designed for knockdown or overexpression of AK039862. To simulate the coexisting dopaminergic cells and microglia cells in vitro, we applied several non-contact co-culture models, including conditioned medium and Transwell co-culture systems. Cytotoxicity of PQ was evaluated using bv2 cells with the concentrations: 30, 60 μM, and mn9d cells with the concentrations: 50, 100 μM. As a result, we depicted multiple interesting individual and interactive features of inflammatory lncRNA AK039862 involved in PQ-induced cellular functional effects.
First, we detected that AK039862 contributed to the neuronal injury process in PQ-treated mice and co-localization of AK039862 with dopaminergic cells in vivo. And interestingly, we demonstrated that PQ significantly inhibited microglia and dopaminergic cells proliferation and microglia migration in vitro. Further research indicated that the PQ-induced low expression of AK039862 rescued microglia proliferation and migration inhibition via the AK039862/Pafah1b1/Foxa1 pathway.
Meanwhile, AK039862 also participated in the interaction between microglia and dopaminergic cells with PQ treatment in non-contact co-culture models. In summary, we found that PQ inhibited the proliferation and migration of microglial cells, and elucidated AK039862 played a key role in PQ-induced neuroinflammatory damage through Pafah1b1/Foxa1. Finally, inflammatory AK039862 is involved in the complex communication between microglia and dopaminergic cells in the environment of PQ damage.

Human Cord Blood CD34+ Cells Derived Endothelial Cells
HEC23 500,000+ Cells
EUR 1080

Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells Growth Medium
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9-46110 5 x 100 ml Ask for price

Human Umbilical Cord Tissue Preparation Buffer 1: Normal Umbilical Artery Endothelial Cells
9-80106 1 x 100 ml Ask for price

Human Umbilical Cord Tissue Preparation Buffer 4: Normal Umbilical Vein Endothelial Cells
9-80109 1 x 100 ml Ask for price

Fetal Umbilical Cord Lysate
XBL-10430 0.1 mg
EUR 416.75
Description: Fetal human umbilical cord tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human umbilical cord tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the umbilical cord tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The umbilical cord tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Medium
9-25087 5 x 100 ml Ask for price

Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Medium
9-32091 5 x 100 ml Ask for price

Mouse Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells
9-80205 1 x 100 ml Ask for price

Rat Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells
9-80294 1 x 100 ml Ask for price

Human Umbilical Cord Tissue Preparation Buffer 2: Normal Umbilical Artery Smooth Muscle Cells
9-80107 1 x 100 ml Ask for price

Human Umbilical Cord Tissue Preparation Buffer 5: Normal Umbilical Vein Smooth Muscle Cells
9-80110 1 x 100 ml Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, single donor
CBCD34-C0.5M - Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, single donor 
CBCD34-C1M - Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor 
CBCD34mix-C0.5M - Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor 
CBCD34mix-C1M - Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor 
CBCD34mix-C5M - Ask for price

Positively Selected Normal Peripheral Blood CD19+ B Cells, Cryopreserved
PBCD19P-C10M - Ask for price

Positively Selected Normal Peripheral Blood CD19+ B Cells, Fresh
PBCD19P-F10M - Ask for price

human umbilical vein endothelial cells, Peptide Aptamer, Biotinylated
AP-316-B 1 mg Ask for price

Human Umbilical Cord OptiTDS1: Tissue Dissociation System
4-28106 1 Kit Ask for price

Human Umbilical Cord OptiTDS2: Tissue Dissociation System
4-28107 1 Kit Ask for price

Human Umbilical Cord OptiTDS4: Tissue Dissociation System
4-28109 1 Kit Ask for price

Human Umbilical Cord OptiTDS5: Tissue Dissociation System
4-28110 1 Kit Ask for price

Human Umbilical Cord PrimaCell1: Normal Umbilical Artery Endothelial Cells Growth Supplements with Serum (for 500 ml medium)
9-47106 1 Set Ask for price

Human Umbilical Cord PrimaCell4: Normal Umbilical Vein Endothelial Cells Growth Supplements with Serum (for 500 ml medium)
9-47109 1 Set Ask for price

Human ; Umbilical Vein Endothelial Cells
02-701 100ng
EUR 401
Description: The Human ; Umbilical Vein Endothelial Cells is available in Europe and for worldwide shipping via Gentaur.

Human Umbilical Vein Endothelial Cells
HEC01 500,000+ Cells frozen
EUR 440

Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-47107 1 Set Ask for price

Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-47110 1 Set Ask for price

Mouse Umbilical Cord OptiTDS: Tissue Dissociation System
4-28205 1 Kit Ask for price

Rat Umbilical Cord OptiTDS: Tissue Dissociation System
4-28294 1 Kit Ask for price

VEGF-stimulated human umbilical vein endothelial cells, Peptide Aptamer, Biotinylated
AP-339-B 1 mg Ask for price

Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-26087 1 Set Ask for price

Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-33091 1 Set Ask for price

Cancer Antigen AFP Antigen (Human Cord Blood)
VAng-Wyb8624-inquire inquire Ask for price
Description: Alpha Fetoprotein (66 kDa), Cancer antigen from Human Cord Blood, 1.73 mg/mL.

Human  Cord  Blood  CD34+  Cells (Mixed  Donors)
CBCD34M-05 - Ask for price

Human  Cord  Blood  CD34+  Cells (Mixed  Donors)
CBCD34M-1 - Ask for price

Human  Cord  Blood  CD34+  Cells (Mixed  Donors)
CBCD34M-5 - Ask for price

GFP Expressing Human Umbilical Vein Endothelial Cells
GF01 500,000+ Cells frozen
EUR 1040

Immortalized Human Umbilical Vein Endothelial Cells - SV40
T0014 1x106 cells / 1.0 ml Ask for price

Human Umbilical Cord FibrOut1: Fibroblast Inhibitory System (for 500 ml medium)
7-15095 1 Kit Ask for price

Human Umbilical Cord FibrOut2: Fibroblast Inhibitory System (for 500 ml medium)
7-15096 1 Kit Ask for price

Human Umbilical Cord FibrOut4: Fibroblast Inhibitory System (for 500 ml medium)
7-15098 1 Kit Ask for price

Human Umbilical Cord FibrOut5: Fibroblast Inhibitory System (for 500 ml medium)
7-15099 1 Kit Ask for price

Human B-lymphocyte antigen CD19 (CD19)
1-CSB-CF004888HU
  • EUR 1298.00
  • EUR 682.00
  • EUR 798.00
  • 1MG
  • 200ug
  • 500ug
Description: Recombinant Human B-lymphocyte antigen CD19(CD19),partial expressed in in vitro E.coli expression system

Human B-lymphocyte antigen CD19 (CD19)
1-CSB-CF004888HUc7
  • EUR 965.00
  • EUR 665.00
  • EUR 715.00
  • 1MG
  • 200ug
  • 500ug
Description: Recombinant Human B-lymphocyte antigen CD19(CD19),partial expressed in in vitro E.coli expression system

human umbilical vein endothelial cells, Peptide Aptamer, unlabeled
AP-316-U 5 mg Ask for price

Sheep Red Blood Cells
20R-RS001 10 ml
EUR 521
Description: Human IgG Senstitized Gluteraldehyde Stabilized Sheep Red Bllood Cells

Bovine Red Blood Cells
88R-B002 20 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Bovine Red Blood Cells

Chicken Red Blood Cells
88R-C002 20 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Chicken Red Blood Cells

Dog Red Blood Cells
88R-D004 10 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Dog Red Blood Cells

Goat Red Blood Cells
88R-G003 20 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Goat Red Blood Cells

Hamster Red Blood Cells
88R-H001 5 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Hamster Red Blood Cells

Horse Red Blood Cells
88R-H002 20 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Horse Red Blood Cells

Hamster Red Blood Cells
88R-H003 10 ml
EUR 489
Description: Washed and freshly prepared 10% suspension of Hamster Red Blood Cells

Mouse Red Blood Cells
88R-M001 10 ml
EUR 295
Description: Washed and freshly prepared 10% suspension of Mouse Red Blood Cells

Monkey Red Blood Cells
88R-M003 5 x 2 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Monkey Red Blood Cells

Mouse Red Blood Cells
88R-M004 5 x 2 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Mouse Red Blood Cells

Pig Red Blood Cells
88R-P002 20 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Pig Red Blood Cells

Rabbit Red Blood Cells
88R-R001 20 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Rabbit Red Blood Cells

Rat Red Blood Cells
88R-R002 5 x 2.0ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Rat Red Blood Cells

Rat Red Blood Cells
88R-R005 25 ml
EUR 327
Description: Washed and freshly prepared 10% suspension of Rat Red Blood Cells

Sheep Red Blood Cells
88R-S001 100 ml
EUR 512
Description: Washed and freshly prepared 10% suspension of Sheep Red Blood Cells

Sheep Red Blood Cells
88R-S003 20 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Sheep Red Blood Cells

Sheep Red Blood Cells
88R-S004 10 ml
EUR 521
Description: Glutaraldehyde-stabilized freshly prepared albumin sensitised Sheep Red Blood Cells

Sheep Red Blood Cells
88R-S005 10 ml
EUR 446
Description: Glutaraldehyde-stabilized freshly prepared tanned Sheep Red Blood Cells

Turkey Red Blood Cells
88R-T001 20 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Turkey Red Blood Cells

Mouse Monoclonal Anti-Human CD19 FITC/CD2 PE
CD19-D1-50 50 tests
EUR 651

Mouse Monoclonal Anti-Human CD19 FITC/CD3 PE
CD19-D2-50 50 tests
EUR 651

Mouse Monoclonal Anti-Human CD19 FITC/CD5 PE
CD19-D3-50 50 tests
EUR 651

Unprocessed Human Cord Blood from IRB-approved donors, Fresh
FC-001-F10 - Ask for price

Unprocessed Human Cord Blood from IRB-approved donors, Fresh
FC-001-F100 - Ask for price

Unprocessed Human Cord Blood from IRB-approved donors, Fresh
FC-001-F50 - Ask for price

Unprocessed Human Cord Blood from IRB-approved donors, Fresh
FC-001-F80 - Ask for price

Mouse Umbilical Cord FibrOut: Fibroblast Inhibitory System (for 500 ml medium)
7-15184 1 Kit Ask for price

Rat Umbilical Cord FibrOut: Fibroblast Inhibitory System (for 500 ml medium)
7-15264 1 Kit Ask for price

human umbilical vein endothelial cells, Peptide Aptamer, FITC labelled
AP-316-F 1 mg Ask for price

CORNING®HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS, HUVEC-2 CELLS (>5X105 CELLS), 1 CRYOVIAL/PACK
354151 1/pk
EUR 388
Description: Permeable Support - DL; Coated Transwells - DL

B-Lymphocyte Antigen CD19 (CD19) Antibody
abx011636-100ul 100 ul
EUR 411

B-Lymphocyte Antigen CD19 (CD19) Antibody
abx159376-100ul 100 ul
EUR 356

B-Lymphocyte Antigen CD19 (CD19) Antibody
abx159377-100ul 100 ul
EUR 356

B-Lymphocyte Antigen CD19 (CD19) Antibody
abx159378-100ul 100 ul
EUR 356

B-Lymphocyte Antigen CD19 (CD19) Antibody
abx159379-100ul 100 ul
EUR 356

B-Lymphocyte Antigen CD19 (CD19) Antibody
abx159380-100ul 100 ul
EUR 439

B-Lymphocyte Antigen CD19 (CD19) Antibody
abx159381-100ul 100 ul
EUR 356

Rabbit Red Blood Cells (100%)
20-abx800015
  • EUR 648.00
  • EUR 509.00
  • 100 ml
  • 50 ml

Sheep Red Blood Cells (100%)
20-abx800032
  • EUR 356.00
  • EUR 314.00
  • 100 ml
  • 50 ml
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