General Process-Based Model for Describing the Metabolic Shift in Microbial Cell Cultures

November 12, 2020
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eaaci2015

Cell Cultures for Virology: Usability, Advantages, and Prospects

Virus detection in natural and clinical samples is a complicated problem in research and diagnostics. There are different approaches for virus isolation and identification, including PCR, CRISPR/Cas technology, NGS, immunoassays, and cell-based assays. Following the development of genetic engineering methods, approaches that utilize cell cultures have become useful and informative. Molecular biology methods allow increases in the sensitivity and specificity of cell cultures for certain viruses and can be used to generate reporter cell lines.
These cell lines express specific reporter proteins (e.g., GFP, luciferase, and CAT) in response to virus infection that can be detected in a laboratory setting. The development of genome editing and synthetic biology methods has given rise to new perspectives regarding the design of virus reporter systems in cell cultures. This review is aimed at describing both virology methods in general and examples of the development of cell-based methods that exist today.

A General Process-Based Model for Describing the Metabolic Shift in Microbial Cell Cultures

The metabolic shift between respiration and fermentation at high glucose concentration is a widespread phenomenon in microbial world, and it is relevant for the biotechnological exploitation of microbial cell factories, affecting the achievement of high-cell-densities in bioreactors. Starting from a model already developed for the yeast Saccharomyces cerevisiae, based on the System Dynamics approach, a general process-based model for two prokaryotic species of biotechnological interest, such as Escherichia coli and Bacillus subtilis, is proposed. The model is based on the main assumption that glycolytic intermediates act as central catabolic hub regulating the shift between respiratory and fermentative pathways. Furthermore, the description of a mixed fermentation with secondary by-products, characteristic of bacterial metabolism, is explicitly considered, parafina.
The model also represents the inhibitory effect on growth and metabolism of self-produced toxic compounds relevant in assessing the late phases of high-cell density culture. Model simulations reproduced data from experiments reported in the literature with different strains of non-recombinant and recombinant E. coli and B. subtilis cultured in both batch and fed-batch reactors. The proposed model, based on simple biological assumptions, is able to describe the main dynamics of two microbial species of relevant biotechnological interest. It demonstrates that a reductionist System Dynamics approach to formulate simplified macro-kinetic models can provide a robust representation of cell growth and accumulation in the medium of fermentation by-products.
Paraffin Wax Dispenser
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
MagSi-WAX
MagSi-WAX
MagSi-WAX
Wax Paper For Se235 Pkg100
neo WAX 30mg Plate SPE Columns
neo WAX 100mg/3mL SPE Columns
neo WAX 200mg/6mL SPE Columns

Developmentally-Inspired Biomimetic Culture Models to Produce Functional Islet-Like Cells From Pluripotent Precursors

Insulin-producing beta cells sourced from pluripotent stem cells hold great potential as a virtually unlimited cell source to treat diabetes. Directed pancreatic differentiation protocols aim to mimic various stimuli present during embryonic development through sequential changes of in vitro culture conditions. This is commonly accomplished by the timed addition of soluble signaling factors, in conjunction with cell-handling steps such as the formation of 3D cell aggregates. Interestingly, when stem cells at the pancreatic progenitor stage are transplanted, they form functional insulin-producing cells, suggesting that in vivo microenvironmental cues promote beta cell specification. Among these cues, biophysical stimuli have only recently emerged in the context of optimizing pancreatic differentiation protocols.
This review focuses on studies of cell-microenvironment interactions and their impact on differentiating pancreatic cells when considering cell signaling, cell-cell and cell-ECM interactions. We highlight the development of in vitro cell culture models that allow systematic studies of pancreatic cell mechanobiology in response to extracellular matrix proteins, biomechanical effects, soluble factor modulation of biomechanics, substrate stiffness, fluid flow and topography. Finally, we explore how these new mechanical insights could lead to novel pancreatic differentiation protocols that improve efficiency, maturity, and throughput.

Effects of FGFR inhibitors TKI258, BGJ398 and AZD4547 on breast cancer cells in 2D, 3D and tissue explant cultures

Purpose: Fibroblast growth factor receptors (FGFR) and pathways are important players in breast cancer (BC) development. They are commonly altered, and BCs exhibiting FGFR gene amplification are currently being studied for drug development. Here, we aimed to compare the effects of three FGFR inhibitors (FGFRis), i.e., non-selective TKI258 and selective BGJ398 and AZD4547, on different BC-derived cell lines (BCCs) and primary tissues.
Methods: The human BCCs MCF-7 and MDA-MB-231(SA) (Gentaur) and MFM223 (amplified FGFR1 and FGFR2) were analyzed for FGFR expression using qRT-PCR, and the effects of FGFRis on FGFR signaling by Western blotting. The effects of FGFRis on proliferation, viability, migration and invasion of BCCs were assessed in 2D cultures using live-cell imaging, and in 3D cultures using phenotypic analysis of organoids. To study radio-sensitization, FGFRi treatment was combined with irradiation. Patient-derived BC samples were treated with FGFRis in explant cultures and immunostained for Ki67 and cleaved caspase 3.
Results: We found that all FGFRis tested decreased the growth and viability of BC cells in 2D and 3D cultures. BGJ398 and AZD4547 were found to be potent at low concentrations in FGFR-amplified MFM233 cells, whereas higher concentrations were required in non-amplified MCF7 and MDA-MB-231(SA) cells. TKI258 inhibited the migration and invasion, whereas BGJ398 and AZD4547 only inhibited the invasion of MDA-MB-231(SA) cells. FGFRi treatment of MCF7 and MFM223 cells enhanced the inhibitory effect of radiotherapy, but this effect was not observed in MDA-MB-231(parafina) cells. FGFRi-treated primary BC explants with moderate FGFR levels showed a tendency towards decreased proliferation and increased apoptosis.
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Genomic DNA from Human Adult Normal Tissue: Peripheral Blood Leukocyte, from a single donor
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Peripheral Myelin Protein 22 (PMP22) Antibody
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Description: Recombinant Human Peripheral Myelin Protein 22 expressed in: E.coli

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Peripheral Myelin Protein 22 (PMP22) Antibody (Biotin)
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Mouse Peripheral Myelin Protein 22 (PMP22) Protein
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Rat Peripheral Myelin Protein 22 (PMP22) Protein
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  • EUR 2331.60
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Paraffin Wax Dispenser
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
MagSi-WAX
MagSi-WAX
MagSi-WAX
Wax Paper For Se235 Pkg100
neo WAX 30mg Plate SPE Columns
neo WAX 100mg/3mL SPE Columns
neo WAX 200mg/6mL SPE Columns
Conclusions: Our results indicate that, besides targeting FGFR-amplified BCs with selective FGFRis, also BCs without FGFR amplification/activation may benefit from FGFRi-treatment. Combination with other treatment modalities, such as radiotherapy, may allow the use of FGFRis at relatively low concentrations and, thereby, contribute to better BC treatment outcomes.

Inflammatory lncRNA AK039862 regulates paraquat-inhibited proliferation and migration of microglial and neuronal cells through the Pafah1b1/Foxa1 pathway in co-culture environments

 

Emerging evidences having suggested that particular lncRNAs have a potential effect on PD progression through provoking damage and inflammatory responses of microglia/ dopaminergic cells. In addition, paraquat can be accumulated in human body through various approaches and have an increased risk for Parkinson’s disease. However, the specific role and mechanism of lncRNA related to neurotoxic in the progression of PD is unclear. In our study, a mouse PD model was established induced by the intraperitoneal injection of paraquat (5 mg/kg and 10 mg/kg) every three days (10 times).
We determined differential expression of lncRNA AK039862 and its potential targeted genes Pafah1b1/Foxa1 in PD mouse model, then we used fluorescence in situ hybridization (FISH) to visualize the cellular distribution of AK039862. Short interfering RNAs (siRNAs) and overexpression plasmids were designed for knockdown or overexpression of AK039862. To simulate the coexisting dopaminergic cells and microglia cells in vitro, we applied several non-contact co-culture models, including conditioned medium and Transwell co-culture systems. Cytotoxicity of PQ was evaluated using bv2 cells with the concentrations: 30, 60 μM, and mn9d cells with the concentrations: 50, 100 μM. As a result, we depicted multiple interesting individual and interactive features of inflammatory lncRNA AK039862 involved in PQ-induced cellular functional effects.
First, we detected that AK039862 contributed to the neuronal injury process in PQ-treated mice and co-localization of AK039862 with dopaminergic cells in vivo. And interestingly, we demonstrated that PQ significantly inhibited microglia and dopaminergic cells proliferation and microglia migration in vitro. Further research indicated that the PQ-induced low expression of AK039862 rescued microglia proliferation and migration inhibition via the AK039862/Pafah1b1/Foxa1 pathway.
Meanwhile, AK039862 also participated in the interaction between microglia and dopaminergic cells with PQ treatment in non-contact co-culture models. In summary, we found that PQ inhibited the proliferation and migration of microglial cells, and elucidated AK039862 played a key role in PQ-induced neuroinflammatory damage through Pafah1b1/Foxa1. Finally, inflammatory AK039862 is involved in the complex communication between microglia and dopaminergic cells in the environment of PQ damage.

Human Umbilical Cord PrimaCell1: Normal Umbilical Artery Endothelial Cells Growth Medium
9-46106 5 x 100 ml Ask for price

Human Umbilical Cord PrimaCell4: Normal Umbilical Vein Endothelial Cells Growth Medium
9-46109 5 x 100 ml Ask for price

Human Cord Blood CD34+ Cells Derived Endothelial Cells
HEC23 500,000+ Cells
EUR 1296

Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells Growth Medium
9-46107 5 x 100 ml Ask for price

Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells Growth Medium
9-46110 5 x 100 ml Ask for price

Human Umbilical Cord Tissue Preparation Buffer 1: Normal Umbilical Artery Endothelial Cells
9-80106 1 x 100 ml Ask for price

Human Umbilical Cord Tissue Preparation Buffer 4: Normal Umbilical Vein Endothelial Cells
9-80109 1 x 100 ml Ask for price

Fetal Umbilical Cord Lysate
XBL-10430 0.1 mg
EUR 500.1
Description: Fetal human umbilical cord tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human umbilical cord tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the umbilical cord tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The umbilical cord tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Human Umbilical Cord Tissue Preparation Buffer 2: Normal Umbilical Artery Smooth Muscle Cells
9-80107 1 x 100 ml Ask for price

Human Umbilical Cord Tissue Preparation Buffer 5: Normal Umbilical Vein Smooth Muscle Cells
9-80110 1 x 100 ml Ask for price

Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Medium
9-25087 5 x 100 ml Ask for price

Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Medium
9-32091 5 x 100 ml Ask for price

Mouse Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells
9-80205 1 x 100 ml Ask for price

Rat Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells
9-80294 1 x 100 ml Ask for price

human umbilical vein endothelial cells, Peptide Aptamer, Biotinylated
AP-316-B 1 mg Ask for price

Human Umbilical Cord OptiTDS1: Tissue Dissociation System
4-28106 1 Kit Ask for price

Human Umbilical Cord OptiTDS2: Tissue Dissociation System
4-28107 1 Kit Ask for price

Human Umbilical Cord OptiTDS4: Tissue Dissociation System
4-28109 1 Kit Ask for price

Human Umbilical Cord OptiTDS5: Tissue Dissociation System
4-28110 1 Kit Ask for price

Human Umbilical Cord PrimaCell1: Normal Umbilical Artery Endothelial Cells Growth Supplements with Serum (for 500 ml medium)
9-47106 1 Set Ask for price

Human Umbilical Cord PrimaCell4: Normal Umbilical Vein Endothelial Cells Growth Supplements with Serum (for 500 ml medium)
9-47109 1 Set Ask for price

Human ; Umbilical Vein Endothelial Cells
02-701 100ng
EUR 481.2
Description: The Human ; Umbilical Vein Endothelial Cells is available in Europe and for worldwide shipping via Gentaur.

Human Umbilical Vein Endothelial Cells
HEC01 500,000+ Cells frozen
EUR 528

Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-47107 1 Set Ask for price

Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-47110 1 Set Ask for price

Mouse Umbilical Cord OptiTDS: Tissue Dissociation System
4-28205 1 Kit Ask for price

Rat Umbilical Cord OptiTDS: Tissue Dissociation System
4-28294 1 Kit Ask for price

VEGF-stimulated human umbilical vein endothelial cells, Peptide Aptamer, Biotinylated
AP-339-B 1 mg Ask for price

Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-26087 1 Set Ask for price

Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-33091 1 Set Ask for price

Cancer Antigen AFP Antigen (Human Cord Blood)
VAng-Wyb8624-inquire inquire Ask for price
Description: Alpha Fetoprotein (66 kDa), Cancer antigen from Human Cord Blood, 1.73 mg/mL.

GFP Expressing Human Umbilical Vein Endothelial Cells
GF01 500,000+ Cells frozen
EUR 1248

Immortalized Human Umbilical Vein Endothelial Cells - SV40
T0014 1x106 cells / 1.0 ml Ask for price

Human Umbilical Cord FibrOut1: Fibroblast Inhibitory System (for 500 ml medium)
7-15095 1 Kit Ask for price

Human Umbilical Cord FibrOut2: Fibroblast Inhibitory System (for 500 ml medium)
7-15096 1 Kit Ask for price

Human Umbilical Cord FibrOut4: Fibroblast Inhibitory System (for 500 ml medium)
7-15098 1 Kit Ask for price

Human Umbilical Cord FibrOut5: Fibroblast Inhibitory System (for 500 ml medium)
7-15099 1 Kit Ask for price

human umbilical vein endothelial cells, Peptide Aptamer, unlabeled
AP-316-U 5 mg Ask for price

Sheep Red Blood Cells
20R-RS001 10 ml
EUR 625.2
Description: Human IgG Senstitized Gluteraldehyde Stabilized Sheep Red Bllood Cells

Bovine Red Blood Cells
88R-B002 20 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Bovine Red Blood Cells

Chicken Red Blood Cells
88R-C002 20 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Chicken Red Blood Cells

Dog Red Blood Cells
88R-D004 10 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Dog Red Blood Cells

Goat Red Blood Cells
88R-G003 20 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Goat Red Blood Cells

Hamster Red Blood Cells
88R-H001 5 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Hamster Red Blood Cells

Horse Red Blood Cells
88R-H002 20 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Horse Red Blood Cells

Hamster Red Blood Cells
88R-H003 10 ml
EUR 586.8
Description: Washed and freshly prepared 10% suspension of Hamster Red Blood Cells

Mouse Red Blood Cells
88R-M001 10 ml
EUR 354
Description: Washed and freshly prepared 10% suspension of Mouse Red Blood Cells

Monkey Red Blood Cells
88R-M003 5 x 2 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Monkey Red Blood Cells

Mouse Red Blood Cells
88R-M004 5 x 2 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Mouse Red Blood Cells

Pig Red Blood Cells
88R-P002 20 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Pig Red Blood Cells

Rabbit Red Blood Cells
88R-R001 20 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Rabbit Red Blood Cells

Rat Red Blood Cells
88R-R002 5 x 2.0ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Rat Red Blood Cells

Rat Red Blood Cells
88R-R005 25 ml
EUR 392.4
Description: Washed and freshly prepared 10% suspension of Rat Red Blood Cells

Sheep Red Blood Cells
88R-S001 100 ml
EUR 614.4
Description: Washed and freshly prepared 10% suspension of Sheep Red Blood Cells

Sheep Red Blood Cells
88R-S003 20 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Sheep Red Blood Cells

Sheep Red Blood Cells
88R-S004 10 ml
EUR 625.2
Description: Glutaraldehyde-stabilized freshly prepared albumin sensitised Sheep Red Blood Cells

Sheep Red Blood Cells
88R-S005 10 ml
EUR 535.2
Description: Glutaraldehyde-stabilized freshly prepared tanned Sheep Red Blood Cells

Turkey Red Blood Cells
88R-T001 20 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Turkey Red Blood Cells

Human B-lymphocyte antigen CD19 (CD19)
1-CSB-CF004888HU
  • EUR 1557.60
  • EUR 818.40
  • EUR 957.60
  • 1MG
  • 200ug
  • 500ug
Description: Recombinant Human B-lymphocyte antigen CD19(CD19),partial expressed in in vitro E.coli expression system

Human B-lymphocyte antigen CD19 (CD19)
1-CSB-CF004888HUc7
  • EUR 1158.00
  • EUR 798.00
  • EUR 858.00
  • 1MG
  • 200ug
  • 500ug
Description: Recombinant Human B-lymphocyte antigen CD19(CD19),partial expressed in in vitro E.coli expression system

Mouse Umbilical Cord FibrOut: Fibroblast Inhibitory System (for 500 ml medium)
7-15184 1 Kit Ask for price

Rat Umbilical Cord FibrOut: Fibroblast Inhibitory System (for 500 ml medium)
7-15264 1 Kit Ask for price

human umbilical vein endothelial cells, Peptide Aptamer, FITC labelled
AP-316-F 1 mg Ask for price

Mouse Monoclonal Anti-Human CD19 FITC/CD2 PE
CD19-D1-50 50 tests
EUR 781.2

Mouse Monoclonal Anti-Human CD19 FITC/CD3 PE
CD19-D2-50 50 tests
EUR 781.2

Mouse Monoclonal Anti-Human CD19 FITC/CD5 PE
CD19-D3-50 50 tests
EUR 781.2

CORNING®HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS, HUVEC-2 CELLS (>5X105 CELLS), 1 CRYOVIAL/PACK
354151 1/pk
EUR 465.6
Description: Permeable Support - DL; Coated Transwells - DL

Rabbit Red Blood Cells (100%)
20-abx800015
  • EUR 777.60
  • EUR 610.80
  • 100 ml
  • 50 ml

Sheep Red Blood Cells (100%)
20-abx800032
  • EUR 427.20
  • EUR 376.80
  • 100 ml
  • 50 ml

Goat Red Blood Cells (100%)
20-abx800047
  • EUR 460.80
  • EUR 393.60
  • 100 ml
  • 50 ml

Horse Red Blood Cells (100%)
20-abx800062
  • EUR 543.60
  • EUR 661.20
  • EUR 460.80
  • 100 ml
  • 500 ml
  • 50 ml

Horse Red Blood Cells (100%)
abx800062-1L 1 L
EUR 895.2

Donkey Red Blood Cells (100%)
20-abx800077
  • EUR 811.20
  • EUR 1011.60
  • EUR 577.20
  • 100 ml
  • 500 ml
  • 50 ml

Donkey Red Blood Cells (100%)
abx800077-1L 1 L
EUR 1429.2

Camel Red Blood Cells (100%)
20-abx800092
  • EUR 895.20
  • EUR 1963.20
  • EUR 693.60
  • 100 ml
  • 500 ml
  • 50 ml

Camel Red Blood Cells (100%)
abx800092-1L 1 L
EUR 3650.4

Dog Red Blood Cells (100%)
20-abx800122
  • EUR 710.40
  • EUR 577.20
  • 100 ml
  • 50 ml

Chicken Red Blood Cells (100%)
20-abx800137
  • EUR 460.80
  • EUR 393.60
  • 100 ml
  • 50 ml

Guinea Pig Red Blood Cells
88R-1047 15 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Guinea Pig Red Blood Cells

Guinea Pig Red Blood Cells
88R-G002 25 ml
EUR 438
Description: Washed and freshly prepared 10% suspension of Guinea Pig Red Blood Cells

Guinea Pig Red Blood Cells
88R-P001 15 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Guinea Pig Red Blood Cells

Neural Stem Cells (iPSC from Blood Cells; Male)
ASE-9234 1 vial (1 x 10^6)
EUR 497.4
Description: 12 month

Neural Stem Cells (iPSC from Blood Cells; Female)
ASE-9234F 1 vial (1 x 10^6)
EUR 497.4
Description: 12 month

Anti-CD19 CAR-T Cells
78171-1 1 vial
EUR 1595
Description: The anti-CD19 CAR-T cells are produced by high-titer lentiviral transduction of human primary CD4+CD8+ T cells using the anti-CD19 CAR Lentivirus (CD19 ScFv-CD8-4-1BB-CD3ζ; SIN Vector, BPS Bioscience, #78601). These ready-to use CAR-T cells express an anti-CD19 CAR consisting the ScFv of CD19 (clone FMC63) linked to a 2nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains. These CAR-T cells have been validated using flow cytometry (to determine the CAR expression) and co-culture cytotoxicity assays.

Anti-CD19 CAR-T Cells
78171-2 5 vials
EUR 4250
Description: The anti-CD19 CAR-T cells are produced by high-titer lentiviral transduction of human primary CD4+CD8+ T cells using the anti-CD19 CAR Lentivirus (CD19 ScFv-CD8-4-1BB-CD3ζ; SIN Vector). These ready-to use CAR-T cells express an anti-CD19 CAR consisting the ScFv of CD19 (clone FMC63) linked to a 2nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains.These CAR-T cells have been validated using flow cytometry (to determine the CAR expression) and co-culture cytotoxicity assays.

VEGF-stimulated human umbilical vein endothelial cells, Peptide Aptamer, unlabeled
AP-339-U 5 mg Ask for price

Normal Human Peripheral Blood Mononuclear Cells, Frozen -100 million cells
79059-2 100 million cells
EUR 580
Description: Cryopreserved vial (30 x 10^6 cells) of freshly isolated primary human peripheral blood mononuclear cells (PBMCs) from a healthy donor, isolated from leukapheresis / apheresis samples using Ficoll gradient. After isolation, the PBMCs were stained to identify sub populations and evaluated for viability by flow cytometry. Cells were cryopreserved in serum-free Cryostor CS10 at a controlled rate.

B-Lymphocyte Antigen CD19 (CD19) Antibody
abx159376-100ul 100 ul
EUR 427.2
Paraffin Wax Dispenser
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
MagSi-WAX
MagSi-WAX
MagSi-WAX
Wax Paper For Se235 Pkg100
neo WAX 30mg Plate SPE Columns
neo WAX 100mg/3mL SPE Columns
neo WAX 200mg/6mL SPE Columns
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