Cell Cultures for Virology: Usability, Advantages, and Prospects
Virus detection in natural and clinical samples is a complicated problem in research and diagnostics. There are different approaches for virus isolation and identification, including PCR, CRISPR/Cas technology, NGS, immunoassays, and cell-based assays. Following the development of genetic engineering methods, approaches that utilize cell cultures have become useful and informative. Molecular biology methods allow increases in the sensitivity and specificity of cell cultures for certain viruses and can be used to generate reporter cell lines.
These cell lines express specific reporter proteins (e.g., GFP, luciferase, and CAT) in response to virus infection that can be detected in a laboratory setting. The development of genome editing and synthetic biology methods has given rise to new perspectives regarding the design of virus reporter systems in cell cultures. This review is aimed at describing both virology methods in general and examples of the development of cell-based methods that exist today.
A General Process-Based Model for Describing the Metabolic Shift in Microbial Cell Cultures
The metabolic shift between respiration and fermentation at high glucose concentration is a widespread phenomenon in microbial world, and it is relevant for the biotechnological exploitation of microbial cell factories, affecting the achievement of high-cell-densities in bioreactors. Starting from a model already developed for the yeast Saccharomyces cerevisiae, based on the System Dynamics approach, a general process-based model for two prokaryotic species of biotechnological interest, such as Escherichia coli and Bacillus subtilis, is proposed. The model is based on the main assumption that glycolytic intermediates act as central catabolic hub regulating the shift between respiratory and fermentative pathways. Furthermore, the description of a mixed fermentation with secondary by-products, characteristic of bacterial metabolism, is explicitly considered.
The model also represents the inhibitory effect on growth and metabolism of self-produced toxic compounds relevant in assessing the late phases of high-cell density culture. Model simulations reproduced data from experiments reported in the literature with different strains of non-recombinant and recombinant E. coli and B. subtilis cultured in both batch and fed-batch reactors. The proposed model, based on simple biological assumptions, is able to describe the main dynamics of two microbial species of relevant biotechnological interest. It demonstrates that a reductionist System Dynamics approach to formulate simplified macro-kinetic models can provide a robust representation of cell growth and accumulation in the medium of fermentation by-products.
Developmentally-Inspired Biomimetic Culture Models to Produce Functional Islet-Like Cells From Pluripotent Precursors
Insulin-producing beta cells sourced from pluripotent stem cells hold great potential as a virtually unlimited cell source to treat diabetes. Directed pancreatic differentiation protocols aim to mimic various stimuli present during embryonic development through sequential changes of in vitro culture conditions. This is commonly accomplished by the timed addition of soluble signaling factors, in conjunction with cell-handling steps such as the formation of 3D cell aggregates. Interestingly, when stem cells at the pancreatic progenitor stage are transplanted, they form functional insulin-producing cells, suggesting that in vivo microenvironmental cues promote beta cell specification. Among these cues, biophysical stimuli have only recently emerged in the context of optimizing pancreatic differentiation protocols.
This review focuses on studies of cell-microenvironment interactions and their impact on differentiating pancreatic cells when considering cell signaling, cell-cell and cell-ECM interactions. We highlight the development of in vitro cell culture models that allow systematic studies of pancreatic cell mechanobiology in response to extracellular matrix proteins, biomechanical effects, soluble factor modulation of biomechanics, substrate stiffness, fluid flow and topography. Finally, we explore how these new mechanical insights could lead to novel pancreatic differentiation protocols that improve efficiency, maturity, and throughput.
Effects of FGFR inhibitors TKI258, BGJ398 and AZD4547 on breast cancer cells in 2D, 3D and tissue explant cultures
Purpose: Fibroblast growth factor receptors (FGFR) and pathways are important players in breast cancer (BC) development. They are commonly altered, and BCs exhibiting FGFR gene amplification are currently being studied for drug development. Here, we aimed to compare the effects of three FGFR inhibitors (FGFRis), i.e., non-selective TKI258 and selective BGJ398 and AZD4547, on different BC-derived cell lines (BCCs) and primary tissues.
Methods: The human BCCs MCF-7 and MDA-MB-231(SA) (wild-type FGFR) and MFM223 (amplified FGFR1 and FGFR2) were analyzed for FGFR expression using qRT-PCR, and the effects of FGFRis on FGFR signaling by Western blotting. The effects of FGFRis on proliferation, viability, migration and invasion of BCCs were assessed in 2D cultures using live-cell imaging, and in 3D cultures using phenotypic analysis of organoids. To study radio-sensitization, FGFRi treatment was combined with irradiation. Patient-derived BC samples were treated with FGFRis in explant cultures and immunostained for Ki67 and cleaved caspase 3.
Results: We found that all FGFRis tested decreased the growth and viability of BC cells in 2D and 3D cultures. BGJ398 and AZD4547 were found to be potent at low concentrations in FGFR-amplified MFM233 cells, whereas higher concentrations were required in non-amplified MCF7 and MDA-MB-231(SA) cells. TKI258 inhibited the migration and invasion, whereas BGJ398 and AZD4547 only inhibited the invasion of MDA-MB-231(SA) cells. FGFRi treatment of MCF7 and MFM223 cells enhanced the inhibitory effect of radiotherapy, but this effect was not observed in MDA-MB-231(SA) cells. FGFRi-treated primary BC explants with moderate FGFR levels showed a tendency towards decreased proliferation and increased apoptosis.

eaaci2015
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh |
PBMC-F100M |
101Bio |
NULL |
EUR 0 |
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh |
PBMC-F1B |
101Bio |
NULL |
EUR 0 |
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh |
PBMC-F2B |
101Bio |
NULL |
EUR 0 |
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh |
PBMC-F300M |
101Bio |
NULL |
EUR 0 |
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh |
BMC-F500M |
101Bio |
NULL |
EUR 0 |
Human Peripheral Blood Mononuclear Cell Isolation and Viability Kit |
K541-30 |
Biovision |
|
EUR 376 |
Human Peripheral Blood CD3+Â T Cells, Cryopreserved |
PBCD3-C10M |
101Bio |
NULL |
EUR 0 |
Human Peripheral Blood CD3+Â T Cells, Cryopreserved |
PBCD3-C20M |
101Bio |
NULL |
EUR 0 |
Human Peripheral Blood CD3+Â T Cells, Fresh |
PBCD3-F20M |
101Bio |
NULL |
EUR 0 |
Positively Selected Normal Peripheral Blood CD19+ B Cells, Cryopreserved |
PBCD19P-C10M |
101Bio |
NULL |
EUR 0 |
Positively Selected Normal Peripheral Blood CD19+ B Cells, Fresh |
PBCD19P-F10M |
101Bio |
NULL |
EUR 0 |
Immortalized Human Bone Marrow Mononuclear Cells |
T0526 |
ABM |
1x106 cells / 1.0 ml |
Ask for price |
Human Normal Peripheral Blood CD4+/CD25+ Regulatory T Cells (T reg), Cryopreserved |
PBCD4-25-C2M |
101Bio |
NULL |
EUR 0 |
Human Normal Peripheral Blood CD4+/CD25+ Regulatory T Cells (T reg), Fresh |
PBCD4-25-F2M |
101Bio |
NULL |
EUR 0 |
Bovine Red Blood Cells |
88R-B002 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Bovine Red Blood Cells |
Chicken Red Blood Cells |
88R-C002 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Chicken Red Blood Cells |
Dog Red Blood Cells |
88R-D004 |
Fitzgerald |
10 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Dog Red Blood Cells |
Goat Red Blood Cells |
88R-G003 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Goat Red Blood Cells |
Hamster Red Blood Cells |
88R-H001 |
Fitzgerald |
5 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Hamster Red Blood Cells |
Horse Red Blood Cells |
88R-H002 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Horse Red Blood Cells |
Hamster Red Blood Cells |
88R-H003 |
Fitzgerald |
10 ml |
EUR 489 |
Description: Washed and freshly prepared 10% suspension of Hamster Red Blood Cells |
Mouse Red Blood Cells |
88R-M001 |
Fitzgerald |
10 ml |
EUR 295 |
Description: Washed and freshly prepared 10% suspension of Mouse Red Blood Cells |
Monkey Red Blood Cells |
88R-M003 |
Fitzgerald |
5 x 2 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Monkey Red Blood Cells |
Mouse Red Blood Cells |
88R-M004 |
Fitzgerald |
5 x 2 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Mouse Red Blood Cells |
Pig Red Blood Cells |
88R-P002 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Pig Red Blood Cells |
Rabbit Red Blood Cells |
88R-R001 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Rabbit Red Blood Cells |
Rat Red Blood Cells |
88R-R002 |
Fitzgerald |
5 x 2.0ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Rat Red Blood Cells |
Rat Red Blood Cells |
88R-R005 |
Fitzgerald |
25 ml |
EUR 327 |
Description: Washed and freshly prepared 10% suspension of Rat Red Blood Cells |
Sheep Red Blood Cells |
88R-S001 |
Fitzgerald |
100 ml |
EUR 512 |
Description: Washed and freshly prepared 10% suspension of Sheep Red Blood Cells |
Sheep Red Blood Cells |
88R-S003 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Sheep Red Blood Cells |
Sheep Red Blood Cells |
88R-S004 |
Fitzgerald |
10 ml |
EUR 521 |
Description: Glutaraldehyde-stabilized freshly prepared albumin sensitised Sheep Red Blood Cells |
Sheep Red Blood Cells |
88R-S005 |
Fitzgerald |
10 ml |
EUR 446 |
Description: Glutaraldehyde-stabilized freshly prepared tanned Sheep Red Blood Cells |
Turkey Red Blood Cells |
88R-T001 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Turkey Red Blood Cells |
Sheep Red Blood Cells |
20R-RS001 |
Fitzgerald |
10 ml |
EUR 521 |
Description: Human IgG Senstitized Gluteraldehyde Stabilized Sheep Red Bllood Cells |
Human Peripheral blood leukocyte (PBL's, WBC) lysate |
HCL-1371 |
Alpha Diagnostics |
100ug |
EUR 238 |
Rabbit Red Blood Cells (100%) |
20-abx800015 |
Abbexa |
|
|
- Shipped within 1-5 working days.
|
Sheep Red Blood Cells (100%) |
20-abx800032 |
Abbexa |
|
|
- Shipped within 1-5 working days.
|
Goat Red Blood Cells (100%) |
20-abx800047 |
Abbexa |
|
|
- Shipped within 1-5 working days.
|
Horse Red Blood Cells (100%) |
20-abx800062 |
Abbexa |
-
EUR 453.00
-
EUR 551.00
-
EUR 384.00
|
|
- Shipped within 1-5 working days.
|
Horse Red Blood Cells (100%) |
abx800062-1L |
Abbexa |
1 L |
EUR 746 |
- Shipped within 1-5 working days.
|
Donkey Red Blood Cells (100%) |
20-abx800077 |
Abbexa |
-
EUR 676.00
-
EUR 843.00
-
EUR 481.00
|
|
- Shipped within 1-5 working days.
|
Donkey Red Blood Cells (100%) |
abx800077-1L |
Abbexa |
1 L |
EUR 1191 |
- Shipped within 1-5 working days.
|
Camel Red Blood Cells (100%) |
20-abx800092 |
Abbexa |
-
EUR 746.00
-
EUR 1636.00
-
EUR 578.00
|
|
- Shipped within 1-5 working days.
|
Camel Red Blood Cells (100%) |
abx800092-1L |
Abbexa |
1 L |
EUR 3042 |
- Shipped within 1-5 working days.
|
Dog Red Blood Cells (100%) |
20-abx800122 |
Abbexa |
|
|
- Shipped within 1-5 working days.
|
Chicken Red Blood Cells (100%) |
20-abx800137 |
Abbexa |
|
|
- Shipped within 1-5 working days.
|
Guinea Pig Red Blood Cells |
88R-1047 |
Fitzgerald |
15 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Guinea Pig Red Blood Cells |
Guinea Pig Red Blood Cells |
88R-G002 |
Fitzgerald |
25 ml |
EUR 365 |
Description: Washed and freshly prepared 10% suspension of Guinea Pig Red Blood Cells |
Guinea Pig Red Blood Cells |
88R-P001 |
Fitzgerald |
15 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Guinea Pig Red Blood Cells |
Neural Stem Cells (iPSC from Blood Cells; Male) |
ASE-9234 |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 414.5 |
Description: 12 month |
Neural Stem Cells (iPSC from Blood Cells; Female) |
ASE-9234F |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 414.5 |
Description: 12 month |
Human Cord Blood CD34+ Cells Derived Endothelial Cells |
HEC23 |
Neuromics |
500,000+ Cells |
EUR 1080 |
cDNA from Human Adult Normal Tissue: Peripheral Blood Leukocyte |
C1234148-10 |
Biochain |
10 reactions |
EUR 231 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Beagle dog Red Blood Cells (100%) |
20-abx800107 |
Abbexa |
|
|
- Shipped within 1-5 working days.
|
Neurons (iPSC from Blood Cells; Male) |
ASE-9321 |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 313.25 |
Description: 12 month |
Neurons (iPSC from Blood Cells; Female) |
ASE-9321F |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 313.25 |
Description: 12 month |
IMEMS Random Nanofibers 6 Chambers |
3D00011 |
Neuromics |
700 nm-random PCL inserts |
EUR 96 |
IMEMS Aligned Nanofibers 6 Chambers |
3D00017 |
Neuromics |
2 750 nm-aligned PCL inserts |
EUR 440 |
Methylated & Low-Methylated DNA Matching Pair - Human Peripheral Blood Leukocyte |
D6234148-PP |
Biochain |
2x5 ug |
EUR 343 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Total Protein from Human Adult Normal Tissue: Peripheral Blood Leukocyte |
P1234148 |
Biochain |
1 mg |
EUR 356 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
Membrane Protein from Human Adult Normal Tissue: Peripheral Blood Leukocyte |
P3234148 |
Biochain |
0.1 mg |
EUR 516 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
Total RNA from Human Adult Normal Tissue: Peripheral Blood Leukocyte |
R1234148-10 |
Biochain |
10 ug |
EUR 194 |
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Rat Anti Rat Rt6.1 (Peripheral T Cells) Monoclonal Antibody |
CABT-49825RR |
Creative Diagnostics |
2 ml |
EUR 533 |
Astrocytes Mature (iPSC from Blood Cells; Male) |
ASE-9322M |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 455 |
Description: 12 month |
Astrocytes Mature (iPSC from Blood Cells; Female) |
ASE-9322MF |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 455 |
Description: 12 month |
Astrocytes Precursors (iPSC from Blood Cells; Male) |
ASE-9322P |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 374 |
Description: 12 month |
Astrocytes Precursors (iPSC from Blood Cells; Female) |
ASE-9322PF |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 374 |
Description: 12 month |
Dopaminergic Neurons (iPSC from Blood Cells; Male) |
ASE-9323 |
Applied StemCell |
1 vial |
EUR 374 |
Description: 12 month |
Dopaminergic Neurons (iPSC from Blood Cells; Female) |
ASE-9323F |
Applied StemCell |
1 vial |
EUR 374 |
Description: 12 month |
96 Well Human Adult Normal Genomic DNA Plate - Peripheral Blood Leukocyte |
D8234148-1 |
Biochain |
1 plate |
EUR 256 |
Human  Peripheral  Blood  CD14+ Monocytes, Cryopreserved / Untouched |
PBCD14U-C10M |
101Bio |
NULL |
EUR 0 |
Human  Peripheral  Blood  CD14+ Monocytes, Fresh / Untouched |
PBCD14U-F10M |
101Bio |
NULL |
EUR 0 |
Human Cord Blood CD34+ Cells, Cryopreserved, single donor |
CBCD34-C0.5M |
101Bio |
NULL |
EUR 0 |
Human Cord Blood CD34+ Cells, Cryopreserved, single donor |
CBCD34-C1M |
101Bio |
NULL |
EUR 0 |
Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor |
CBCD34mix-C0.5M |
101Bio |
NULL |
EUR 0 |
Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor |
CBCD34mix-C1M |
101Bio |
NULL |
EUR 0 |
Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor |
CBCD34mix-C5M |
101Bio |
NULL |
EUR 0 |
Neurons Starter Kit (iPSC from Blood Cells; Male) |
ASE-9321K |
Applied StemCell |
1 Kit |
EUR 394.25 |
Description: 12 month |
Neurons Starter Kit (iPSC from Blood Cells; Female) |
ASE-9321KF |
Applied StemCell |
1 Kit |
EUR 394.25 |
Description: 12 month |
Human  Peripheral  Blood  CD14+ Monocytes, Cryopreserved / Positive selection |
PBCD14P-C10M |
101Bio |
NULL |
EUR 0 |
Human  Peripheral  Blood  CD14+ Monocytes, Fresh / Positive selection |
PBCD14P-F10M |
101Bio |
NULL |
EUR 0 |
Astrocytes Precursors Starter Kit (iPSC from Blood Cells; Male) |
ASE-9322PK |
Applied StemCell |
1 Kit |
EUR 414.5 |
Description: 12 month |
Astrocytes Precursors Starter Kit (iPSC from Blood Cells; Female) |
ASE-9322PKF |
Applied StemCell |
1 Kit |
EUR 414.5 |
Description: 12 month |
Dopaminergic Neurons Starter Kit (iPSC from Blood Cells; Male) |
ASE-9323K |
Applied StemCell |
1 vial |
EUR 455 |
Description: 12 month |
Dopaminergic Neurons Starter Kit (iPSC from Blood Cells; Female) |
ASE-9323KF |
Applied StemCell |
1 vial |
EUR 455 |
Description: 12 month |
Mouse Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells |
2-82072 |
CHI Scientific |
1 Kit |
Ask for price |
Rat Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells |
2-82571 |
CHI Scientific |
1 Kit |
Ask for price |
Human Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells |
2-96088 |
CHI Scientific |
1 Kit |
Ask for price |
Human  Peripheral  Blood  CD4+  T  Lymphocytes, Cryopreserved |
PBCD4-C10M |
101Bio |
NULL |
EUR 0 |
Human  Peripheral  Blood  CD4+  T  Lymphocytes, Fresh |
PBCD4-F10M |
101Bio |
NULL |
EUR 0 |
Human  Peripheral  Blood  CD4+  T  Lymphocytes, Fresh |
PBCD4-F20M |
101Bio |
NULL |
EUR 0 |
Anti-CD3/CD28 stimulus for human PBMC |
CT372 |
U-CyTech |
50 tests |
EUR 118 |
Genomic DNA from Human Adult Normal Tissue: Peripheral Blood Leukocyte, from a single donor |
D1234148 |
Biochain |
100 ug |
EUR 282 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Human Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells Growth Medium |
9-46088 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Rat Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells Growth Medium |
9-25071 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Mouse Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells Growth Medium |
9-32072 |
CHI Scientific |
5 x 100 ml |
Ask for price |
HL-60 Cell Slide (Human (36yrs, Female) peripheral blood promyeloblast, acute promyelocytic leukemia) (5 slides/pk) |
HCLS-17009 |
Alpha Diagnostics |
1 pk |
EUR 250 |
Human Lymph Tissue Preparation Buffer 2: Normal Lymphatic Blood Vessel Endothelial Cells |
9-80088 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Mouse Lymph Tissue Preparation Buffer 2: Normal Lymphatic Blood Vessel Endothelial Cells |
9-80186 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Rat Lymph Tissue Preparation Buffer 2: Normal Lymphatic Blood Vessel Endothelial Cells |
9-80278 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Human  Cord  Blood  CD34+  Cells (Mixed  Donors) |
CBCD34M-05 |
101Bio |
NULL |
EUR 0 |
Human  Cord  Blood  CD34+  Cells (Mixed  Donors) |
CBCD34M-1 |
101Bio |
NULL |
EUR 0 |
Human  Cord  Blood  CD34+  Cells (Mixed  Donors) |
CBCD34M-5 |
101Bio |
NULL |
EUR 0 |
iPSC (iPSC from Blood Cells; Male, Master Lines for Neural Differentiation) *Academic Price |
ASE-9109 |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 1568.75 |
Description: 12 month |
iPSC (iPSC from Blood Cells; Feale, Master Lines for Neural Differentiation) *Academic Price |
ASE-9110 |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 1568.75 |
Description: 12 month |
digital dry bath, with two independently controlled chambers, without blocks, 115V |
BCM1411 |
Bio Basic |
1 pcs, 1 UNIT |
EUR 978.77 |
- Product category: Benchtop Equipment/Heat Baths/Dry Baths
|
THP-1 Cell Slide (Human (1 yr infant, Male) peripheral blood monocyte, acute monocytic leukemia) (5 slides/pk) |
HCLS-17008 |
Alpha Diagnostics |
1 pk |
EUR 250 |
Peripheral Myelin Protein 2 (PMP2) Antibody |
20-abx114394 |
Abbexa |
|
|
- Shipped within 5-10 working days.
|
Peripheral Myelin Protein 22 (PMP22) Antibody |
20-abx131854 |
Abbexa |
-
EUR 342.00
-
EUR 857.00
-
EUR 439.00
-
EUR 154.00
-
EUR 258.00
|
-
100 ug
-
1 mg
-
200 ug
-
20 ug
-
50 ug
|
- Shipped within 5-7 working days.
|
Peripheral Myelin Protein 22 (PMP22) Antibody |
20-abx133606 |
Abbexa |
-
EUR 300.00
-
EUR 439.00
-
EUR 189.00
|
|
- Shipped within 5-10 working days.
|
Peripheral Myelin Protein 22 (PMP22) Antibody |
20-abx103057 |
Abbexa |
-
EUR 425.00
-
EUR 133.00
-
EUR 1205.00
-
EUR 578.00
-
EUR 328.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
- Shipped within 5-7 working days.
|
Peripheral Myelin Protein 22 (PMP22) Antibody |
abx028584-400ul |
Abbexa |
400 ul |
EUR 523 |
- Shipped within 5-10 working days.
|
Peripheral Myelin Protein 22 (PMP22) Antibody |
abx028584-80l |
Abbexa |
80 µl |
EUR 286 |
- Shipped within 5-10 working days.
|
Peripheral Myelin Protein 22 (PMP22) Antibody |
20-abx173998 |
Abbexa |
|
|
|
Peripheral Myelin Protein 22 (PMP22) Antibody |
20-abx177954 |
Abbexa |
|
|
|
Peripheral Myelin Protein 22 (PMP22) Antibody |
20-abx177955 |
Abbexa |
|
|
|
Peripheral Myelin Protein 22 (PMP22) Antibody |
20-abx177956 |
Abbexa |
-
EUR 453.00
-
EUR 133.00
-
EUR 1302.00
-
EUR 620.00
-
EUR 342.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
- Shipped within 5-12 working days.
|
Peripheral Myelin Protein 22 (PMP22) Antibody |
20-abx328779 |
Abbexa |
|
|
- Shipped within 5-10 working days.
|
Peripheral Myelin Protein 22 (PMP22) Antibody |
20-abx217831 |
Abbexa |
|
|
- Shipped within 5-10 working days.
|
Peripheral Myelin Protein 22 (PMP22) Antibody |
20-abx304860 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
-
100 ug
-
1 mg
-
200 ug
-
20 ug
-
50 ug
|
- Shipped within 5-10 working days.
|
Recombinant Human Peripheral Myelin Protein-2 |
7-05908 |
CHI Scientific |
5µg |
Ask for price |
Recombinant Human Peripheral Myelin Protein-2 |
7-05909 |
CHI Scientific |
25µg |
Ask for price |
Recombinant Human Peripheral Myelin Protein-2 |
7-05910 |
CHI Scientific |
1mg |
Ask for price |
Rat Peripheral myelin protein 22 (Pmp22) |
1-CSB-CF018241RA |
Cusabio |
-
EUR 1208.00
-
EUR 482.00
-
EUR 738.00
|
|
- MW: 23.4 kDa
- Buffer composition: Tris-based buffer with 50% glycerol.
|
Description: Recombinant Rat Peripheral myelin protein 22(Pmp22) expressed in in vitro E.coli expression system |
Recombinant mouse Peripheral myelin protein 22 |
P1724 |
FN Test |
100ug |
Ask for price |
- Uniprot ID: P16646
- Reconstitution: Metal affinity chromatography on Fn Super Capacity Column (Nickel)
|
Description: Recombinant protein for mouse Peripheral myelin protein 22 |
Recombinant rat Peripheral myelin protein 22 |
P1725 |
FN Test |
100ug |
Ask for price |
- Uniprot ID: P25094
- Reconstitution: Metal affinity chromatography on Fn Super Capacity Column (Nickel)
|
Description: Recombinant protein for rat Peripheral myelin protein 22 |
Conclusions: Our results indicate that, besides targeting FGFR-amplified BCs with selective FGFRis, also BCs without FGFR amplification/activation may benefit from FGFRi-treatment. Combination with other treatment modalities, such as radiotherapy, may allow the use of FGFRis at relatively low concentrations and, thereby, contribute to better BC treatment outcomes.
Inflammatory lncRNA AK039862 regulates paraquat-inhibited proliferation and migration of microglial and neuronal cells through the Pafah1b1/Foxa1 pathway in co-culture environments
Emerging evidences having suggested that particular lncRNAs have a potential effect on PD progression through provoking damage and inflammatory responses of microglia/ dopaminergic cells. In addition, paraquat can be accumulated in human body through various approaches and have an increased risk for Parkinson’s disease. However, the specific role and mechanism of lncRNA related to neurotoxic in the progression of PD is unclear. In our study, a mouse PD model was established induced by the intraperitoneal injection of paraquat (5 mg/kg and 10 mg/kg) every three days (10 times).
We determined differential expression of lncRNA AK039862 and its potential targeted genes Pafah1b1/Foxa1 in PD mouse model, then we used fluorescence in situ hybridization (FISH) to visualize the cellular distribution of AK039862. Short interfering RNAs (siRNAs) and overexpression plasmids were designed for knockdown or overexpression of AK039862. To simulate the coexisting dopaminergic cells and microglia cells in vitro, we applied several non-contact co-culture models, including conditioned medium and Transwell co-culture systems. Cytotoxicity of PQ was evaluated using bv2 cells with the concentrations: 30, 60 μM, and mn9d cells with the concentrations: 50, 100 μM. As a result, we depicted multiple interesting individual and interactive features of inflammatory lncRNA AK039862 involved in PQ-induced cellular functional effects.
First, we detected that AK039862 contributed to the neuronal injury process in PQ-treated mice and co-localization of AK039862 with dopaminergic cells in vivo. And interestingly, we demonstrated that PQ significantly inhibited microglia and dopaminergic cells proliferation and microglia migration in vitro. Further research indicated that the PQ-induced low expression of AK039862 rescued microglia proliferation and migration inhibition via the AK039862/Pafah1b1/Foxa1 pathway.
Meanwhile, AK039862 also participated in the interaction between microglia and dopaminergic cells with PQ treatment in non-contact co-culture models. In summary, we found that PQ inhibited the proliferation and migration of microglial cells, and elucidated AK039862 played a key role in PQ-induced neuroinflammatory damage through Pafah1b1/Foxa1. Finally, inflammatory AK039862 is involved in the complex communication between microglia and dopaminergic cells in the environment of PQ damage.
Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells |
2-82091 |
CHI Scientific |
1 Kit |
Ask for price |
Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells |
2-82587 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord PrimaCell1: Normal Umbilical Artery Endothelial Cells Growth Medium |
9-46106 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Human Umbilical Cord PrimaCell4: Normal Umbilical Vein Endothelial Cells Growth Medium |
9-46109 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Human Cord Blood CD34+ Cells Derived Endothelial Cells |
HEC23 |
Neuromics |
500,000+ Cells |
EUR 1080 |
Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells Growth Medium |
9-46107 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells Growth Medium |
9-46110 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Human Umbilical Cord Tissue Preparation Buffer 1: Normal Umbilical Artery Endothelial Cells |
9-80106 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Human Umbilical Cord Tissue Preparation Buffer 4: Normal Umbilical Vein Endothelial Cells |
9-80109 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Mouse Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells |
9-80205 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Rat Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells |
9-80294 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Medium |
9-25087 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Medium |
9-32091 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Human Umbilical Cord Tissue Preparation Buffer 2: Normal Umbilical Artery Smooth Muscle Cells |
9-80107 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Human Umbilical Cord Tissue Preparation Buffer 5: Normal Umbilical Vein Smooth Muscle Cells |
9-80110 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Human Cord Blood CD34+ Cells, Cryopreserved, single donor |
CBCD34-C0.5M |
101Bio |
NULL |
EUR 0 |
Human Cord Blood CD34+ Cells, Cryopreserved, single donor |
CBCD34-C1M |
101Bio |
NULL |
EUR 0 |
Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor |
CBCD34mix-C0.5M |
101Bio |
NULL |
EUR 0 |
Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor |
CBCD34mix-C1M |
101Bio |
NULL |
EUR 0 |
Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor |
CBCD34mix-C5M |
101Bio |
NULL |
EUR 0 |
Positively Selected Normal Peripheral Blood CD19+ B Cells, Cryopreserved |
PBCD19P-C10M |
101Bio |
NULL |
EUR 0 |
Positively Selected Normal Peripheral Blood CD19+ B Cells, Fresh |
PBCD19P-F10M |
101Bio |
NULL |
EUR 0 |
human umbilical vein endothelial cells, Peptide Aptamer, Biotinylated |
AP-316-B |
Alpha Diagnostics |
1 mg |
Ask for price |
Human Umbilical Cord OptiTDS1: Tissue Dissociation System |
4-28106 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord OptiTDS2: Tissue Dissociation System |
4-28107 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord OptiTDS4: Tissue Dissociation System |
4-28109 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord OptiTDS5: Tissue Dissociation System |
4-28110 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord PrimaCell1: Normal Umbilical Artery Endothelial Cells Growth Supplements with Serum (for 500 ml medium) |
9-47106 |
CHI Scientific |
1 Set |
Ask for price |
Human Umbilical Cord PrimaCell4: Normal Umbilical Vein Endothelial Cells Growth Supplements with Serum (for 500 ml medium) |
9-47109 |
CHI Scientific |
1 Set |
Ask for price |
Human Umbilical Vein Endothelial Cells |
HEC01 |
Neuromics |
500,000+ Cells frozen |
EUR 440 |
Human ; Umbilical Vein Endothelial Cells |
02-701 |
Sceti |
100ng |
EUR 401 |
Description: The Human ; Umbilical Vein Endothelial Cells is available in Europe and for worldwide shipping via Gentaur. |
Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium) |
9-47107 |
CHI Scientific |
1 Set |
Ask for price |
Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium) |
9-47110 |
CHI Scientific |
1 Set |
Ask for price |
Mouse Umbilical Cord OptiTDS: Tissue Dissociation System |
4-28205 |
CHI Scientific |
1 Kit |
Ask for price |
Rat Umbilical Cord OptiTDS: Tissue Dissociation System |
4-28294 |
CHI Scientific |
1 Kit |
Ask for price |
VEGF-stimulated human umbilical vein endothelial cells, Peptide Aptamer, Biotinylated |
AP-339-B |
Alpha Diagnostics |
1 mg |
Ask for price |
Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium) |
9-33091 |
CHI Scientific |
1 Set |
Ask for price |
Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium) |
9-26087 |
CHI Scientific |
1 Set |
Ask for price |
Cancer Antigen AFP Antigen (Human Cord Blood) |
VAng-Wyb8624-inquire |
Creative Biolabs |
inquire |
Ask for price |
Description: Alpha Fetoprotein (66 kDa), Cancer antigen from Human Cord Blood, 1.73 mg/mL. |
Human  Cord  Blood  CD34+  Cells (Mixed  Donors) |
CBCD34M-05 |
101Bio |
NULL |
EUR 0 |
Human  Cord  Blood  CD34+  Cells (Mixed  Donors) |
CBCD34M-1 |
101Bio |
NULL |
EUR 0 |
Human  Cord  Blood  CD34+  Cells (Mixed  Donors) |
CBCD34M-5 |
101Bio |
NULL |
EUR 0 |
GFP Expressing Human Umbilical Vein Endothelial Cells |
GF01 |
Neuromics |
500,000+ Cells frozen |
EUR 1040 |
Immortalized Human Umbilical Vein Endothelial Cells - SV40 |
T0014 |
ABM |
1x106 cells / 1.0 ml |
Ask for price |
Human B-lymphocyte antigen CD19 (CD19) |
1-CSB-CF004888HU |
Cusabio |
-
EUR 1298.00
-
EUR 682.00
-
EUR 798.00
|
|
- MW: 34.1 kDa
- Buffer composition: Tris-based buffer with 50% glycerol.
|
Description: Recombinant Human B-lymphocyte antigen CD19(CD19),partial expressed in in vitro E.coli expression system |
Human B-lymphocyte antigen CD19 (CD19) |
1-CSB-CF004888HUc7 |
Cusabio |
-
EUR 965.00
-
EUR 665.00
-
EUR 715.00
|
|
- MW: 30.8 kDa
- Buffer composition: Tris-based buffer with 50% glycerol.
|
Description: Recombinant Human B-lymphocyte antigen CD19(CD19),partial expressed in in vitro E.coli expression system |
Human Umbilical Cord FibrOut1: Fibroblast Inhibitory System (for 500 ml medium) |
7-15095 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord FibrOut2: Fibroblast Inhibitory System (for 500 ml medium) |
7-15096 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord FibrOut4: Fibroblast Inhibitory System (for 500 ml medium) |
7-15098 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord FibrOut5: Fibroblast Inhibitory System (for 500 ml medium) |
7-15099 |
CHI Scientific |
1 Kit |
Ask for price |
human umbilical vein endothelial cells, Peptide Aptamer, unlabeled |
AP-316-U |
Alpha Diagnostics |
5 mg |
Ask for price |
Bovine Red Blood Cells |
88R-B002 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Bovine Red Blood Cells |
Chicken Red Blood Cells |
88R-C002 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Chicken Red Blood Cells |
Dog Red Blood Cells |
88R-D004 |
Fitzgerald |
10 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Dog Red Blood Cells |
Goat Red Blood Cells |
88R-G003 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Goat Red Blood Cells |
Hamster Red Blood Cells |
88R-H001 |
Fitzgerald |
5 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Hamster Red Blood Cells |
Horse Red Blood Cells |
88R-H002 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Horse Red Blood Cells |
Hamster Red Blood Cells |
88R-H003 |
Fitzgerald |
10 ml |
EUR 489 |
Description: Washed and freshly prepared 10% suspension of Hamster Red Blood Cells |
Mouse Red Blood Cells |
88R-M001 |
Fitzgerald |
10 ml |
EUR 295 |
Description: Washed and freshly prepared 10% suspension of Mouse Red Blood Cells |
Monkey Red Blood Cells |
88R-M003 |
Fitzgerald |
5 x 2 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Monkey Red Blood Cells |
Mouse Red Blood Cells |
88R-M004 |
Fitzgerald |
5 x 2 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Mouse Red Blood Cells |
Pig Red Blood Cells |
88R-P002 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Pig Red Blood Cells |
Rabbit Red Blood Cells |
88R-R001 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Rabbit Red Blood Cells |
Rat Red Blood Cells |
88R-R002 |
Fitzgerald |
5 x 2.0ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Rat Red Blood Cells |
Rat Red Blood Cells |
88R-R005 |
Fitzgerald |
25 ml |
EUR 327 |
Description: Washed and freshly prepared 10% suspension of Rat Red Blood Cells |
Sheep Red Blood Cells |
88R-S001 |
Fitzgerald |
100 ml |
EUR 512 |
Description: Washed and freshly prepared 10% suspension of Sheep Red Blood Cells |
Sheep Red Blood Cells |
88R-S003 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Sheep Red Blood Cells |
Sheep Red Blood Cells |
88R-S004 |
Fitzgerald |
10 ml |
EUR 521 |
Description: Glutaraldehyde-stabilized freshly prepared albumin sensitised Sheep Red Blood Cells |
Sheep Red Blood Cells |
88R-S005 |
Fitzgerald |
10 ml |
EUR 446 |
Description: Glutaraldehyde-stabilized freshly prepared tanned Sheep Red Blood Cells |
Turkey Red Blood Cells |
88R-T001 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Turkey Red Blood Cells |
Sheep Red Blood Cells |
20R-RS001 |
Fitzgerald |
10 ml |
EUR 521 |
Description: Human IgG Senstitized Gluteraldehyde Stabilized Sheep Red Bllood Cells |
Mouse Monoclonal Anti-Human CD19 FITC/CD2 PE |
CD19-D1-50 |
Alpha Diagnostics |
50 tests |
EUR 651 |
Mouse Monoclonal Anti-Human CD19 FITC/CD3 PE |
CD19-D2-50 |
Alpha Diagnostics |
50 tests |
EUR 651 |
Mouse Monoclonal Anti-Human CD19 FITC/CD5 PE |
CD19-D3-50 |
Alpha Diagnostics |
50 tests |
EUR 651 |
Unprocessed Human Cord Blood from IRB-approved donors, Fresh |
FC-001-F10 |
101Bio |
NULL |
EUR 0 |
Unprocessed Human Cord Blood from IRB-approved donors, Fresh |
FC-001-F100 |
101Bio |
NULL |
EUR 0 |
Unprocessed Human Cord Blood from IRB-approved donors, Fresh |
FC-001-F50 |
101Bio |
NULL |
EUR 0 |
Unprocessed Human Cord Blood from IRB-approved donors, Fresh |
FC-001-F80 |
101Bio |
NULL |
EUR 0 |
Mouse Umbilical Cord FibrOut: Fibroblast Inhibitory System (for 500 ml medium) |
7-15184 |
CHI Scientific |
1 Kit |
Ask for price |
Rat Umbilical Cord FibrOut: Fibroblast Inhibitory System (for 500 ml medium) |
7-15264 |
CHI Scientific |
1 Kit |
Ask for price |
human umbilical vein endothelial cells, Peptide Aptamer, FITC labelled |
AP-316-F |
Alpha Diagnostics |
1 mg |
Ask for price |
B-Lymphocyte Antigen CD19 (CD19) Antibody |
abx159376-100ul |
Abbexa |
100 ul |
EUR 356 |
- Shipped within 5-10 working days.
|
B-Lymphocyte Antigen CD19 (CD19) Antibody |
abx159377-100ul |
Abbexa |
100 ul |
EUR 356 |
- Shipped within 5-10 working days.
|
B-Lymphocyte Antigen CD19 (CD19) Antibody |
abx159378-100ul |
Abbexa |
100 ul |
EUR 356 |
- Shipped within 5-10 working days.
|
B-Lymphocyte Antigen CD19 (CD19) Antibody |
abx159379-100ul |
Abbexa |
100 ul |
EUR 356 |
- Shipped within 5-10 working days.
|
B-Lymphocyte Antigen CD19 (CD19) Antibody |
abx159380-100ul |
Abbexa |
100 ul |
EUR 439 |
- Shipped within 5-10 working days.
|
B-Lymphocyte Antigen CD19 (CD19) Antibody |
abx159381-100ul |
Abbexa |
100 ul |
EUR 356 |
- Shipped within 5-10 working days.
|
B-Lymphocyte Antigen CD19 (CD19) Antibody |
abx011636-100ul |
Abbexa |
100 ul |
EUR 411 |
- Shipped within 5-10 working days.
|
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