General Process-Based Model for Describing the Metabolic Shift in Microbial Cell Cultures

November 12, 2020
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eaaci2015

Cell Cultures for Virology: Usability, Advantages, and Prospects

Virus detection in natural and clinical samples is a complicated problem in research and diagnostics. There are different approaches for virus isolation and identification, including PCR, CRISPR/Cas technology, NGS, immunoassays, and cell-based assays. Following the development of genetic engineering methods, approaches that utilize cell cultures have become useful and informative. Molecular biology methods allow increases in the sensitivity and specificity of cell cultures for certain viruses and can be used to generate reporter cell lines.
These cell lines express specific reporter proteins (e.g., GFP, luciferase, and CAT) in response to virus infection that can be detected in a laboratory setting. The development of genome editing and synthetic biology methods has given rise to new perspectives regarding the design of virus reporter systems in cell cultures. This review is aimed at describing both virology methods in general and examples of the development of cell-based methods that exist today.

A General Process-Based Model for Describing the Metabolic Shift in Microbial Cell Cultures

The metabolic shift between respiration and fermentation at high glucose concentration is a widespread phenomenon in microbial world, and it is relevant for the biotechnological exploitation of microbial cell factories, affecting the achievement of high-cell-densities in bioreactors. Starting from a model already developed for the yeast Saccharomyces cerevisiae, based on the System Dynamics approach, a general process-based model for two prokaryotic species of biotechnological interest, such as Escherichia coli and Bacillus subtilis, is proposed. The model is based on the main assumption that glycolytic intermediates act as central catabolic hub regulating the shift between respiratory and fermentative pathways. Furthermore, the description of a mixed fermentation with secondary by-products, characteristic of bacterial metabolism, is explicitly considered, parafina.
The model also represents the inhibitory effect on growth and metabolism of self-produced toxic compounds relevant in assessing the late phases of high-cell density culture. Model simulations reproduced data from experiments reported in the literature with different strains of non-recombinant and recombinant E. coli and B. subtilis cultured in both batch and fed-batch reactors. The proposed model, based on simple biological assumptions, is able to describe the main dynamics of two microbial species of relevant biotechnological interest. It demonstrates that a reductionist System Dynamics approach to formulate simplified macro-kinetic models can provide a robust representation of cell growth and accumulation in the medium of fermentation by-products.
Paraffin Wax Dispenser
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
8KG Histoplast PE Paraffin
Paraffin oil, BP, Ph. Eur. grade
Paraffin oil, BP, Ph. Eur. grade
Paraffin oil, BP, Ph. Eur. grade
Paraffin oil, BP, Ph. Eur. grade

Developmentally-Inspired Biomimetic Culture Models to Produce Functional Islet-Like Cells From Pluripotent Precursors

Insulin-producing beta cells sourced from pluripotent stem cells hold great potential as a virtually unlimited cell source to treat diabetes. Directed pancreatic differentiation protocols aim to mimic various stimuli present during embryonic development through sequential changes of in vitro culture conditions. This is commonly accomplished by the timed addition of soluble signaling factors, in conjunction with cell-handling steps such as the formation of 3D cell aggregates. Interestingly, when stem cells at the pancreatic progenitor stage are transplanted, they form functional insulin-producing cells, suggesting that in vivo microenvironmental cues promote beta cell specification. Among these cues, biophysical stimuli have only recently emerged in the context of optimizing pancreatic differentiation protocols.
This review focuses on studies of cell-microenvironment interactions and their impact on differentiating pancreatic cells when considering cell signaling, cell-cell and cell-ECM interactions. We highlight the development of in vitro cell culture models that allow systematic studies of pancreatic cell mechanobiology in response to extracellular matrix proteins, biomechanical effects, soluble factor modulation of biomechanics, substrate stiffness, fluid flow and topography. Finally, we explore how these new mechanical insights could lead to novel pancreatic differentiation protocols that improve efficiency, maturity, and throughput.

Effects of FGFR inhibitors TKI258, BGJ398 and AZD4547 on breast cancer cells in 2D, 3D and tissue explant cultures

Purpose: Fibroblast growth factor receptors (FGFR) and pathways are important players in breast cancer (BC) development. They are commonly altered, and BCs exhibiting FGFR gene amplification are currently being studied for drug development. Here, we aimed to compare the effects of three FGFR inhibitors (FGFRis), i.e., non-selective TKI258 and selective BGJ398 and AZD4547, on different BC-derived cell lines (BCCs) and primary tissues.
Methods: The human BCCs MCF-7 and MDA-MB-231(SA) (Gentaur) and MFM223 (amplified FGFR1 and FGFR2) were analyzed for FGFR expression using qRT-PCR, and the effects of FGFRis on FGFR signaling by Western blotting. The effects of FGFRis on proliferation, viability, migration and invasion of BCCs were assessed in 2D cultures using live-cell imaging, and in 3D cultures using phenotypic analysis of organoids. To study radio-sensitization, FGFRi treatment was combined with irradiation. Patient-derived BC samples were treated with FGFRis in explant cultures and immunostained for Ki67 and cleaved caspase 3.
Results: We found that all FGFRis tested decreased the growth and viability of BC cells in 2D and 3D cultures. BGJ398 and AZD4547 were found to be potent at low concentrations in FGFR-amplified MFM233 cells, whereas higher concentrations were required in non-amplified MCF7 and MDA-MB-231(SA) cells. TKI258 inhibited the migration and invasion, whereas BGJ398 and AZD4547 only inhibited the invasion of MDA-MB-231(SA) cells. FGFRi treatment of MCF7 and MFM223 cells enhanced the inhibitory effect of radiotherapy, but this effect was not observed in MDA-MB-231(parafina) cells. FGFRi-treated primary BC explants with moderate FGFR levels showed a tendency towards decreased proliferation and increased apoptosis.
eaaci2015

eaaci2015

 

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved
PBMC-C20M - Ask for price

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved
PBMC-C50M - Ask for price

Single Donor Human Peripheral Blood Mononuclear Cells (PBMC) Frozen
IWB6K2E1000CT each
EUR 2281
Description: Single Donor Human Peripheral Blood Mononuclear Cells (PBMC) Frozen

Single Donor Human Peripheral Blood Mononuclear Cells (PBMC) Frozen
IWB6K2E100CT each
EUR 554
Description: Single Donor Human Peripheral Blood Mononuclear Cells (PBMC) Frozen

Single Donor Human Peripheral Blood Mononuclear Cells (PBMC) Frozen
IWB6K2E10CT each
EUR 136
Description: Single Donor Human Peripheral Blood Mononuclear Cells (PBMC) Frozen

Single Donor Human Peripheral Blood Mononuclear Cells (PBMC) Frozen
IWB6K2E25CT each
EUR 265
Description: Single Donor Human Peripheral Blood Mononuclear Cells (PBMC) Frozen

Single Donor Human Peripheral Blood Mononuclear Cells (PBMC) Frozen
IWB6K2E300CT each
EUR 939
Description: Single Donor Human Peripheral Blood Mononuclear Cells (PBMC) Frozen

Single Donor Human Peripheral Blood Mononuclear Cells (PBMC) Frozen
IWB6K2E50CT each
EUR 389
Description: Single Donor Human Peripheral Blood Mononuclear Cells (PBMC) Frozen

Normal Human Peripheral Blood Mononuclear Cells, Frozen
79059-1 30 million cells
EUR 220
Description: Cryopreserved vial (30 x 10^6 cells) of freshly isolated primary human peripheral blood mononuclear cells (PBMCs) from a healthy donor, isolated from leukapheresis / apheresis samples using Ficoll gradient. After isolation, the PBMCs were stained to identify sub populations and evaluated for viability by flow cytometry. Cells were cryopreserved in serum-free Cryostor CS10 at a controlled rate.

Normal Human Peripheral Blood Mononuclear Cells, Frozen -100 million cells
79059-2 100 million cells
EUR 580
Description: Cryopreserved vial (30 x 10^6 cells) of freshly isolated primary human peripheral blood mononuclear cells (PBMCs) from a healthy donor, isolated from leukapheresis / apheresis samples using Ficoll gradient. After isolation, the PBMCs were stained to identify sub populations and evaluated for viability by flow cytometry. Cells were cryopreserved in serum-free Cryostor CS10 at a controlled rate.

Human Peripheral Blood Mononuclear Cell Isolation and Viability Kit
K541-30 each
EUR 451.2

AnaPrep Blood DNA Extraction Kit 1200 (48) For extracting genomic DNA from mammalian whole blood, peripheral mononuclear cell, or buffy coat. Sample volume range: up to 1 ml
Z1322002 1 kit (48 extractions) Including all required plastic disposables
EUR 419

AnaPrep Blood DNA Extraction Kit 200 (48) For extracting genomic DNA from mammalian whole blood, peripheral mononuclear cell, or buffy coat. Sample volume range: up to 400 µl
Z1322001 1 kit (48 extractions) Including all required plastic disposables
EUR 227

Human Cord Blood Mononuclear Cells (CB MNC)
CBMNC-C10M 10 million
EUR 166.8

Human Cord Blood Mononuclear Cells (CB MNC)
CBMNC-C150M 150 million
EUR 1066.8

Human Cord Blood Mononuclear Cells (CB MNC)
CBMNC-C50M 50 million
EUR 718.8

Human Cord Blood Mononuclear Cells (CB MNC)
CBMNC-F100M 100 million
EUR 1438.8

Human Cord Blood Mononuclear Cells (CB MNC)
CBMNC-F200M 200 million
EUR 2038.8

Human Cord Blood Mononuclear Cells (CB MNC)
CBMNC-F50M 50 million
EUR 958.8

Human Normal Peripheral Blood CD56+ NK Cells
PBCD56P-C5M 5 million
EUR 825.6

Human Normal Peripheral Blood CD56+ NK Cells
PBCD56U-C5M 5 million
EUR 1014

Human Normal Peripheral Blood CD56+ NK Cells
PBCD56U-F5M 5 million
EUR 1198.8

Human Peripheral Blood CD3+ T Cells, Fresh
PBCD3-F20M - Ask for price

Human Normal Peripheral Blood CD3+ Pan T Cells
PBCD3-F100M 100 million Ask for price

Human Normal Peripheral Blood CD3+ Pan T Cells
PBCD3-F500M 500 million Ask for price

Human Normal Peripheral Blood CD3+ Pan T Cells
PBCD3-F50M 50 million
EUR 1798.8

Human Peripheral Blood CD3+ T Cells, Cryopreserved
PBCD3-C10M - Ask for price

Human Peripheral Blood CD3+ T Cells, Cryopreserved
PBCD3-C20M - Ask for price

Human Normal Peripheral Blood CD8+ Cytotoxic T Cells
PBCD8-F10M 10 million
EUR 994.8

Human Normal Peripheral Blood CD4+/CD45RO+ Memory T Cells
PBCD4-45RO-C5M 5 million
EUR 1162.8

Human Normal Peripheral Blood CD4+/CD45RO+ Memory T Cells
PBCD4-45RO-F5M 5 million
EUR 1220.4

Human Mobilized Peripheral Blood CD34+ Stem Cells (MPB HSCs)
MPBCD34-C02M 0.2 million
EUR 430.8

Human Mobilized Peripheral Blood CD34+ Stem Cells (MPB HSCs)
MPBCD34-C10M 10 millon
EUR 5148

Human Mobilized Peripheral Blood CD34+ Stem Cells (MPB HSCs)
MPBCD34-C1M 1 million
EUR 1030.8

Human Mobilized Peripheral Blood CD34+ Stem Cells (MPB HSCs)
MPBCD34-C20M 20 million
EUR 9108

Human Mobilized Peripheral Blood CD34+ Stem Cells (MPB HSCs)
MPBCD34-C5M 5 million
EUR 3540

Human Mobilized Peripheral Blood CD34+ Stem Cells (MPB HSCs)
MPBCD34-deplete 100 million
EUR 238.8

Human Mobilized Peripheral Blood CD34+ Stem Cells (MPB HSCs)
MPBCD34-F100M 100 million Ask for price

Human Mobilized Peripheral Blood CD34+ Stem Cells (MPB HSCs)
MPBCD34-F10M 10 millon
EUR 6626.4

Human Mobilized Peripheral Blood CD34+ Stem Cells (MPB HSCs)
MPBCD34-F1M 1 million
EUR 1198.8

Human Mobilized Peripheral Blood CD34+ Stem Cells (MPB HSCs)
MPBCD34-F20M 20 million
EUR 11878.8

Human Mobilized Peripheral Blood CD34+ Stem Cells (MPB HSCs)
MPBCD34-F50M 50 million Ask for price

Human Mobilized Peripheral Blood CD34+ Stem Cells (MPB HSCs)
MPBCD34-F5M 5 million
EUR 4548

Human Bone Marrow Mononuclear Cells (BMMCs)
BMMN-C10M 10 million
EUR 358.8

Human Bone Marrow Mononuclear Cells (BMMCs)
BMMN-C20M 20 million
EUR 478.8

Human Bone Marrow Mononuclear Cells (BMMCs)
BMMN-C50M 50 million
EUR 936

Human Bone Marrow Mononuclear Cells (BMMCs)
BMMN-C5M 5 million
EUR 190.8

Human Bone Marrow Mononuclear Cells (BMMCs)
BMMN-F100M 100 million
EUR 1560

Human Bone Marrow Mononuclear Cells (BMMCs)
BMMN-F200M 200 million
EUR 3000

Human Bone Marrow Mononuclear Cells (BMMCs)
BMMN-F50M 50 million
EUR 828

Human Normal Peripheral Blood CD4+/CD45RA+/CD25- Naive T Cells
PBCD4Naive-C5M 5 million
EUR 1090.8

Human Normal Peripheral Blood CD4+/CD45RA+/CD25- Naive T Cells
PBCD4Naive-F5M 5 million
EUR 1222.8

Immortalized Human Bone Marrow Mononuclear Cells
T0526 1x106 cells / 1.0 ml Ask for price

Human Normal Peripheral Blood CD8+/CD45RA+ Naive Cytotoxic T Cells
PBCD8-45RA-C5M 5 million
EUR 1065.6

Human Normal Peripheral Blood CD8+/CD45RA+ Naive Cytotoxic T Cells
PBCD8-45RA-F5M 5 million
EUR 1318.8

Positively Selected Normal Peripheral Blood CD19+ B Cells, Fresh
PBCD19P-F10M - Ask for price

Positively Selected Normal Peripheral Blood CD19+ B Cells, Cryopreserved
PBCD19P-C10M - Ask for price

Human Normal Peripheral Blood CD4+/CD25+ Regulatory T Cells (T reg), Fresh
PBCD4-25-F2M - Ask for price

Human Normal Peripheral Blood CD14+ Monocytes
PBCD14P-C20M 20 million
EUR 1114.8

Human Normal Peripheral Blood CD14+ Monocytes
PBCD14P-C40M 40 million
EUR 1614

Human Normal Peripheral Blood CD14+ Monocytes
PBCD14P-F20M 20 million
EUR 1402.8

Human Normal Peripheral Blood CD14+ Monocytes
PBCD14P-F40M 40 million
EUR 1678.8

Human Peripheral Blood Leukocyte Cell Lysate
IHULEUTL100UG each
EUR 245
Description: Human Peripheral Blood Leukocyte Cell Lysate

Human Normal Peripheral Blood CD4+/CD25+ Regulatory T Cells (T reg), Cryopreserved
PBCD4-25-C2M - Ask for price

Human Normal Peripheral Blood Basophils
PBB-F03M 0.3 million
EUR 1228.8

Human Normal Peripheral Blood Eosinophils
PBE-F1M 1 million
EUR 1210.8

Human Normal Peripheral Blood Leuko Pak
FP-001-1 Quarter Pak (1/4 bag)
EUR 2158.8

Human Normal Peripheral Blood Leuko Pak
FP-001-2 Half Pak (1/2 bag)
EUR 2347.2

Human Normal Peripheral Blood Leuko Pak
FP-001-3 Full Pak (1 bag)
EUR 3478.8

Peripheral Blood Leukocyte Membrane Lysate
XBL-10813 0.1 mg
EUR 884.4
Description: Human peripheral blood leukocyte tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human peripheral blood leukocyte tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated peripheral blood leukocyte tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated peripheral blood leukocyte tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Human Normal Apheresis Peripheral Blood Plasma
FP-003-10 10 mL
EUR 114

Human Normal Apheresis Peripheral Blood Plasma
FP-003-100 100 mL
EUR 478.8

Human Normal Apheresis Peripheral Blood Plasma
FP-003-20 20 mL
EUR 154.8

Human Normal Apheresis Peripheral Blood Plasma
FP-003-30 30 mL
EUR 201.6

Human Normal Apheresis Peripheral Blood Plasma
FP-003-40 40 mL
EUR 253.2

Human Normal Apheresis Peripheral Blood Plasma
FP-003-50 50 mL
EUR 274.8

Human Peripheral blood leukocyte (PBL's, WBC) lysate
HCL-1371 100ug
EUR 285.6

Human  Peripheral  Blood  CD14+ Monocytes, Fresh / Untouched
PBCD14U-F10M - Ask for price

Human Peripheral Blood Leukocyte Lysate Membrane Fraction
IHULEUTLM100UG each
EUR 1413
Description: Human Peripheral Blood Leukocyte Lysate Membrane Fraction

Human  Peripheral  Blood  CD14+ Monocytes, Cryopreserved / Untouched
PBCD14U-C10M - Ask for price

cDNA - Human Adult Normal Tissue: Peripheral Blood Leukocyte
C1234148-10 10 reactions
EUR 215

Human  Peripheral  Blood  CD14+ Monocytes, Fresh / Positive selection
PBCD14P-F10M - Ask for price

THP-1 Cell Slide (Human (1 yr infant, Male) peripheral blood monocyte, acute monocytic leukemia) (5 slides/pk)
HCLS-17008 1 pk
EUR 300

Total RNA - Human Adult Normal Tissue: Peripheral Blood Leukocyte
R1234148-10 10 ug
EUR 183

Human  Peripheral  Blood  CD14+ Monocytes, Cryopreserved / Positive selection
PBCD14P-C10M - Ask for price

Total Protein - Human Adult Normal Tissue: Peripheral Blood Leukocyte
P1234148 1 mg
EUR 351

Human  Peripheral  Blood  CD4+  T  Lymphocytes, Fresh
PBCD4-F10M - Ask for price

Human  Peripheral  Blood  CD4+  T  Lymphocytes, Fresh
PBCD4-F20M - Ask for price

Membrane Protein - Human Adult Normal Tissue: Peripheral Blood Leukocyte
P3234148 0.1 mg
EUR 535

Rat Anti Rat Rt6.1 (Peripheral T Cells) Monoclonal Antibody
CABT-49825RR 2 ml
EUR 639.6

96 Well Human Adult Normal Genomic DNA Plate - Peripheral Blood Leukocyte
D8234148-1 1 plate
EUR 254

Human  Peripheral  Blood  CD4+  T  Lymphocytes, Cryopreserved
PBCD4-C10M - Ask for price

Methylated & Low-Methylated DNA Matched Pair - Human Peripheral Blood Leukocyte
D6234148-PP 2x5 ug
EUR 351

Genomic DNA - Human Adult Normal Tissue: Peripheral Blood Leukocyte, from a single donor
D1234148 100 ug
EUR 286

HL-60 Cell Slide (Human (36yrs, Female) peripheral blood promyeloblast, acute promyelocytic leukemia) (5 slides/pk)
HCLS-17009 1 pk
EUR 300

Total RNA - Human Adult Normal Tissue: Peripheral Blood Leukocyte, from 4 different donors, 2 male and 2 female. 1 ug/ul concentration
R1234148-10-P04 10x10 µg
EUR 2199

Peripheral nerve tumor tissue array
SO1001a each
EUR 306
Description: Peripheral nerve tumor tissue array, including TNM and clinical stage, 50 cases/100 cores, replaced by SO1001b

Peripheral nerve tumor tissue array
SO1001b each
EUR 306
Description: Peripheral nerve tumor tissue array, including TNM and clinical stage, 50 cases/100 cores, replacing SO1001a

Rat Peripheral myelin protein 22 (Pmp22)
1-CSB-CF018241RA
  • EUR 1449.60
  • EUR 578.40
  • EUR 885.60
  • 1MG
  • 200ug
  • 500ug
Description: Recombinant Rat Peripheral myelin protein 22(Pmp22) expressed in in vitro E.coli expression system

Peripheral Myelin Protein 22 (PMP22) Monoclonal Antibody (Human)
4-MAC746Hu21
  • EUR 306.00
  • EUR 3170.40
  • EUR 786.00
  • EUR 386.40
  • EUR 260.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Peripheral Myelin Protein 22 (PMP22)

Peripheral Myelin Protein 22 (PMP22) Blocking Peptide
20-abx061188
  • EUR 343.20
  • EUR 510.00
  • 1 mg
  • 5 mg

Peripheral Myelin Protein 22 (PMP22) Monoclonal Antibody (Human), PE
4-MAC746Hu21-PE
  • EUR 367.20
  • EUR 3340.80
  • EUR 943.20
  • EUR 463.20
  • EUR 238.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Peripheral Myelin Protein 22 (PMP22). This antibody is labeled with PE.

Peripheral Myelin Protein 2 (PMP2) Antibody
20-abx114394
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Rat Peripheral Myelin Protein 22 ELISA Kit
IRTPMP22KT each
EUR 790
Description: Rat Peripheral Myelin Protein 22 ELISA Kit

Peripheral Myelin Protein 22 (PMP22) Monoclonal Antibody (Human), APC
4-MAC746Hu21-APC
  • EUR 429.60
  • EUR 4146.00
  • EUR 1148.40
  • EUR 549.60
  • EUR 268.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Peripheral Myelin Protein 22 (PMP22). This antibody is labeled with APC.

Peripheral Myelin Protein 22 (PMP22) Monoclonal Antibody (Human), Cy3
4-MAC746Hu21-Cy3
  • EUR 522.00
  • EUR 5478.00
  • EUR 1482.00
  • EUR 682.80
  • EUR 309.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Peripheral Myelin Protein 22 (PMP22). This antibody is labeled with Cy3.

Peripheral Myelin Protein 22 (PMP22) Monoclonal Antibody (Human), HRP
4-MAC746Hu21-HRP
  • EUR 392.40
  • EUR 3613.20
  • EUR 1015.20
  • EUR 495.60
  • EUR 253.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Peripheral Myelin Protein 22 (PMP22). This antibody is labeled with HRP.

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx217831
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx103057
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx173998
  • EUR 1095.60
  • EUR 560.40
  • 1 mg
  • 200 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx177954
  • EUR 1479.60
  • EUR 710.40
  • 1 mg
  • 200 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx177955
  • EUR 1479.60
  • EUR 710.40
  • 1 mg
  • 200 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx177956
  • EUR 543.60
  • EUR 159.60
  • EUR 1562.40
  • EUR 744.00
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx133606
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Peripheral Myelin Protein 22 (PMP22) Antibody
abx028584-400ul 400 ul
EUR 627.6

Peripheral Myelin Protein 22 (PMP22) Antibody
abx028584-80l 80 µl
EUR 343.2

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx131854
  • EUR 410.40
  • EUR 1028.40
  • EUR 526.80
  • EUR 184.80
  • EUR 309.60
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx328779
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx304860
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Peripheral Myelin Protein 22 (PMP22) Monoclonal Antibody (Human), FITC
4-MAC746Hu21-FITC
  • EUR 367.20
  • EUR 3340.80
  • EUR 943.20
  • EUR 463.20
  • EUR 238.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Peripheral Myelin Protein 22 (PMP22). This antibody is labeled with FITC.

Recombinant rat Peripheral myelin protein 22
P1725 100ug Ask for price
Description: Recombinant protein for rat Peripheral myelin protein 22

Human Peripheral Myelin Protein 22 ELISA Kit
IHUPMP22KT each
EUR 733
Description: Human Peripheral Myelin Protein 22 ELISA Kit

Mouse Peripheral Myelin Protein 22 ELISA Kit
IMSPMP22KT each
EUR 752
Description: Mouse Peripheral Myelin Protein 22 ELISA Kit

Recombinant mouse Peripheral myelin protein 22
P1724 100ug Ask for price
Description: Recombinant protein for mouse Peripheral myelin protein 22

Recombinant Peripheral Myelin Protein 22 (PMP22)
4-RPC746Hu01
  • EUR 539.33
  • EUR 267.60
  • EUR 1692.48
  • EUR 644.16
  • EUR 1168.32
  • EUR 436.80
  • EUR 4051.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Peripheral Myelin Protein 22 expressed in: E.coli

Rat Peripheral Myelin Protein 22 (PMP22) Protein
20-abx654683
  • EUR 777.60
  • EUR 326.40
  • EUR 2331.60
  • EUR 910.80
  • EUR 560.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

 

Paraffin Wax Dispenser
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
8KG Histoplast PE Paraffin
Paraffin oil, BP, Ph. Eur. grade
Paraffin oil, BP, Ph. Eur. grade
Paraffin oil, BP, Ph. Eur. grade
Paraffin oil, BP, Ph. Eur. grade
Conclusions: Our results indicate that, besides targeting FGFR-amplified BCs with selective FGFRis, also BCs without FGFR amplification/activation may benefit from FGFRi-treatment. Combination with other treatment modalities, such as radiotherapy, may allow the use of FGFRis at relatively low concentrations and, thereby, contribute to better BC treatment outcomes.

Inflammatory lncRNA AK039862 regulates paraquat-inhibited proliferation and migration of microglial and neuronal cells through the Pafah1b1/Foxa1 pathway in co-culture environments

 

Emerging evidences having suggested that particular lncRNAs have a potential effect on PD progression through provoking damage and inflammatory responses of microglia/ dopaminergic cells. In addition, paraquat can be accumulated in human body through various approaches and have an increased risk for Parkinson’s disease. However, the specific role and mechanism of lncRNA related to neurotoxic in the progression of PD is unclear. In our study, a mouse PD model was established induced by the intraperitoneal injection of paraquat (5 mg/kg and 10 mg/kg) every three days (10 times).
We determined differential expression of lncRNA AK039862 and its potential targeted genes Pafah1b1/Foxa1 in PD mouse model, then we used fluorescence in situ hybridization (FISH) to visualize the cellular distribution of AK039862. Short interfering RNAs (siRNAs) and overexpression plasmids were designed for knockdown or overexpression of AK039862. To simulate the coexisting dopaminergic cells and microglia cells in vitro, we applied several non-contact co-culture models, including conditioned medium and Transwell co-culture systems. Cytotoxicity of PQ was evaluated using bv2 cells with the concentrations: 30, 60 μM, and mn9d cells with the concentrations: 50, 100 μM. As a result, we depicted multiple interesting individual and interactive features of inflammatory lncRNA AK039862 involved in PQ-induced cellular functional effects.
First, we detected that AK039862 contributed to the neuronal injury process in PQ-treated mice and co-localization of AK039862 with dopaminergic cells in vivo. And interestingly, we demonstrated that PQ significantly inhibited microglia and dopaminergic cells proliferation and microglia migration in vitro. Further research indicated that the PQ-induced low expression of AK039862 rescued microglia proliferation and migration inhibition via the AK039862/Pafah1b1/Foxa1 pathway.
Meanwhile, AK039862 also participated in the interaction between microglia and dopaminergic cells with PQ treatment in non-contact co-culture models. In summary, we found that PQ inhibited the proliferation and migration of microglial cells, and elucidated AK039862 played a key role in PQ-induced neuroinflammatory damage through Pafah1b1/Foxa1. Finally, inflammatory AK039862 is involved in the complex communication between microglia and dopaminergic cells in the environment of PQ damage.

Human Umbilical Cord PrimaCell4: Normal Umbilical Vein Endothelial Cells Growth Medium
9-46109 5 x 100 ml Ask for price

Human Umbilical Cord Mesenchymal Stem Cells With GFP
HUXUC-01101 1*10^6/vial
EUR 1599

Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells
2-82587 1 Kit Ask for price

Human Umbilical Cord PrimaCell1: Normal Umbilical Artery Endothelial Cells Growth Medium
9-46106 5 x 100 ml Ask for price

Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells
2-82091 1 Kit Ask for price

Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells Growth Medium
9-46110 5 x 100 ml Ask for price

Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells Growth Medium
9-46107 5 x 100 ml Ask for price

Human Umbilical Cord Tissue Preparation Buffer 4: Normal Umbilical Vein Endothelial Cells
9-80109 1 x 100 ml Ask for price

Human Umbilical Cord Tissue Preparation Buffer 1: Normal Umbilical Artery Endothelial Cells
9-80106 1 x 100 ml Ask for price

Human Umbilical Cord Tissue Preparation Buffer 5: Normal Umbilical Vein Smooth Muscle Cells
9-80110 1 x 100 ml Ask for price

Human Umbilical Cord Tissue Preparation Buffer 2: Normal Umbilical Artery Smooth Muscle Cells
9-80107 1 x 100 ml Ask for price

Complete Medium For Human Umbilical Cord Mesenchymal Stem Cells
HUXUC-90011 500mL
EUR 319

Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Medium
9-25087 5 x 100 ml Ask for price

Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Medium
9-32091 5 x 100 ml Ask for price

Rat Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells
9-80294 1 x 100 ml Ask for price

Mouse Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells
9-80205 1 x 100 ml Ask for price

Serum Free Medium For Human Umbilical Cord Mesenchymal Stem Cells
HUXUC-90061 500mL
EUR 399

Single Donor Human Umbilical Cord
IRHUSCD each
EUR 957
Description: Single Donor Human Umbilical Cord

Fetal Umbilical Cord Lysate
XBL-10430 0.1 mg
EUR 500.1
Description: Fetal human umbilical cord tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human umbilical cord tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the umbilical cord tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The umbilical cord tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Human Umbilical Cord PrimaCell1: Normal Umbilical Artery Endothelial Cells Growth Supplements with Serum (for 500 ml medium)
9-47106 1 Set Ask for price

Human Umbilical Cord PrimaCell4: Normal Umbilical Vein Endothelial Cells Growth Supplements with Serum (for 500 ml medium)
9-47109 1 Set Ask for price

Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-47110 1 Set Ask for price

Osteogenic Differentiation Medium For Human Umbilical Cord Mesenchymal Stem Cells
HUXUC-90021 200mL
EUR 250

Adipogenic Differentiation Medium For Human Umbilical Cord Mesenchymal Stem Cells
HUXUC-90031 200+100mL
EUR 359

Human Umbilical Cord Fetal Tissue Lysate
IHUCDFSTL100UG each
EUR 870
Description: Human Umbilical Cord Fetal Tissue Lysate

Chondrogenic Differentiation Medium For Human Umbilical Cord Mesenchymal Stem Cells
HUXUC-90041 100mL
EUR 409

Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-47107 1 Set Ask for price

Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-26087 1 Set Ask for price

Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-33091 1 Set Ask for price

Human Umbilical Cord OptiTDS1: Tissue Dissociation System
4-28106 1 Kit Ask for price

Human Umbilical Cord OptiTDS2: Tissue Dissociation System
4-28107 1 Kit Ask for price

Human Umbilical Cord OptiTDS4: Tissue Dissociation System
4-28109 1 Kit Ask for price

Human Umbilical Cord OptiTDS5: Tissue Dissociation System
4-28110 1 Kit Ask for price

Human ; Umbilical Vein Endothelial Cells
02-701 100ng
EUR 481.2
Description: The Human ; Umbilical Vein Endothelial Cells is available in Europe and for worldwide shipping via Gentaur.

Human Umbilical Vein Endothelial Cells
HEC01 500,000+ Cells frozen
EUR 528

Human Umbilical Vein Endothelial Cells
HUVEC-20001 1*10^6/vial
EUR 1100

Human Cord Blood CD19+ B Cells
CBCD19-C2.5M 2.5 million
EUR 906

Human Cord Blood CD19+ B Cells
CBCD19-C5M 5 million
EUR 1078.8

Human Cord Blood CD19+ B Cells
CBCD19-F5M 5 million
EUR 1198.8

Innovative Grade US Origin Bovine Umbilical Cord
IGBOCDF each
EUR 210
Description: Innovative Grade US Origin Bovine Umbilical Cord

Innovative Grade US Origin Bovine Umbilical Cord
IGBOCDP each
EUR 210
Description: Innovative Grade US Origin Bovine Umbilical Cord

Innovative Grade US Origin Bovine Umbilical Cord
IGBOCDS each
EUR 133
Description: Innovative Grade US Origin Bovine Umbilical Cord

Innovative Grade US Origin Bovine Umbilical Cord
IGBOCDZ each
EUR 133
Description: Innovative Grade US Origin Bovine Umbilical Cord

Innovative Grade US Origin Porcine Umbilical Cord
IGPCCDF each
EUR 133
Description: Innovative Grade US Origin Porcine Umbilical Cord

Innovative Grade US Origin Porcine Umbilical Cord
IGPCCDP each
EUR 210
Description: Innovative Grade US Origin Porcine Umbilical Cord

Innovative Grade US Origin Porcine Umbilical Cord
IGPCCDS each
EUR 210
Description: Innovative Grade US Origin Porcine Umbilical Cord

Innovative Grade US Origin Porcine Umbilical Cord
IGPCCDZ each
EUR 133
Description: Innovative Grade US Origin Porcine Umbilical Cord

Rat Umbilical Cord OptiTDS: Tissue Dissociation System
4-28294 1 Kit Ask for price

Mouse Umbilical Cord OptiTDS: Tissue Dissociation System
4-28205 1 Kit Ask for price

GFP Expressing Human Umbilical Vein Endothelial Cells
GF01 500,000+ Cells frozen
EUR 1248

Human Umbilical Cord FibrOut1: Fibroblast Inhibitory System (for 500 ml medium)
7-15095 1 Kit Ask for price

Human Umbilical Cord FibrOut2: Fibroblast Inhibitory System (for 500 ml medium)
7-15096 1 Kit Ask for price

Human Umbilical Cord FibrOut4: Fibroblast Inhibitory System (for 500 ml medium)
7-15098 1 Kit Ask for price

Human Umbilical Cord FibrOut5: Fibroblast Inhibitory System (for 500 ml medium)
7-15099 1 Kit Ask for price

Immortalized Human Umbilical Vein Endothelial Cells - SV40
T0014 1x106 cells / 1.0 ml Ask for price

Complete Medium For Human Umbilical Vein Endothelial Cells
HUVEC-90011 500mL
EUR 319

CORNING®HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS, HUVEC-2 CELLS (>5X105 CELLS), 1 CRYOVIAL/PACK
354151 1/pk
EUR 465.6
Description: Permeable Support - DL; Coated Transwells - DL

human umbilical vein endothelial cells, Peptide Aptamer, unlabeled
AP-316-U 5 mg Ask for price

Rat Umbilical Cord FibrOut: Fibroblast Inhibitory System (for 500 ml medium)
7-15264 1 Kit Ask for price

human umbilical vein endothelial cells, Peptide Aptamer, Biotinylated
AP-316-B 1 mg Ask for price

Mouse Umbilical Cord FibrOut: Fibroblast Inhibitory System (for 500 ml medium)
7-15184 1 Kit Ask for price

human umbilical vein endothelial cells, Peptide Aptamer, FITC labelled
AP-316-F 1 mg Ask for price

VEGF-stimulated human umbilical vein endothelial cells, Peptide Aptamer, unlabeled
AP-339-U 5 mg Ask for price

VEGF-stimulated human umbilical vein endothelial cells, Peptide Aptamer, Biotinylated
AP-339-B 1 mg Ask for price

Human Cord Blood CD56+ Nk Cells
CBCD56-C1M 1 million
EUR 886.8

Human Cord Blood CD56+ Nk Cells
CBCD56-F1M 1 million
EUR 994.8

VEGF-stimulated human umbilical vein endothelial cells, Peptide Aptamer, FITC labelled
AP-339-F 1 mg Ask for price

Human Cord Blood CD34+ Cells Derived Endothelial Cells
HEC23 500,000+ Cells
EUR 1296

Human Cord Blood CD3+ Pan T Cells
CBCD3-C10M 10 million
EUR 1020

Human Cord Blood CD3+ Pan T Cells
CBCD3-C20M 20 million
EUR 1560

Human Cord Blood CD3+ Pan T Cells
CBCD3-F10M 10 million
EUR 1068

Human Cord Blood CD3+ Pan T Cells
CBCD3-F20M 20 million
EUR 1800

Human Cord Blood CD34+ Stem / Progenitor Cells
CBCD34-deplete 1-2x10^8
EUR 718.8

Human Cord Blood CD34+ Stem / Progenitor Cells
CBCD34-F1M 1 million
EUR 2580

Human Cord Blood CD34+ Stem / Progenitor Cells
CBCD34mix-F1M 1 million
EUR 1980

Human Cord Blood CD4+/ CD45RA+ Naive T Cells
CBCD4-45RA-C10M 10 million
EUR 1378.8

Human Cord Blood CD4+/ CD45RA+ Naive T Cells
CBCD4-45RA-C15M 15 million
EUR 1678.8

Human Cord Blood CD4+/ CD45RA+ Naive T Cells
CBCD4-45RA-C5M 5 million
EUR 838.8

Human Cord Blood CD4+/ CD45RA+ Naive T Cells
CBCD4-45RA-F10M 10 million
EUR 1798.8

Human Cord Blood CD4+/ CD45RA+ Naive T Cells
CBCD4-45RA-F15M 15 million
EUR 1980

Human Cord Blood CD4+/ CD45RA+ Naive T Cells
CBCD4-45RA-F5M 5 million
EUR 1198.8

Human Cord Blood CD8+ Cytotoxic T Cells
CBCD8-C5M 5 million
EUR 958.8

Human Cord Blood CD8+ Cytotoxic T Cells
CBCD8-F5M 5 million
EUR 1078.8

Human Cord Blood CD34+ Cells, Cryopreserved, single donor
CBCD34-C0.5M - Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor 
CBCD34mix-C0.5M - Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor 
CBCD34mix-C1M - Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor 
CBCD34mix-C5M - Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, single donor 
CBCD34-C1M - Ask for price
Paraffin Wax Dispenser
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
8KG Histoplast PE Paraffin
Paraffin oil, BP, Ph. Eur. grade
Paraffin oil, BP, Ph. Eur. grade
Paraffin oil, BP, Ph. Eur. grade
Paraffin oil, BP, Ph. Eur. grade
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