General Process-Based Model for Describing the Metabolic Shift in Microbial Cell Cultures

November 12, 2020
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eaaci2015

Cell Cultures for Virology: Usability, Advantages, and Prospects

Virus detection in natural and clinical samples is a complicated problem in research and diagnostics. There are different approaches for virus isolation and identification, including PCR, CRISPR/Cas technology, NGS, immunoassays, and cell-based assays. Following the development of genetic engineering methods, approaches that utilize cell cultures have become useful and informative. Molecular biology methods allow increases in the sensitivity and specificity of cell cultures for certain viruses and can be used to generate reporter cell lines.
These cell lines express specific reporter proteins (e.g., GFP, luciferase, and CAT) in response to virus infection that can be detected in a laboratory setting. The development of genome editing and synthetic biology methods has given rise to new perspectives regarding the design of virus reporter systems in cell cultures. This review is aimed at describing both virology methods in general and examples of the development of cell-based methods that exist today.

A General Process-Based Model for Describing the Metabolic Shift in Microbial Cell Cultures

The metabolic shift between respiration and fermentation at high glucose concentration is a widespread phenomenon in microbial world, and it is relevant for the biotechnological exploitation of microbial cell factories, affecting the achievement of high-cell-densities in bioreactors. Starting from a model already developed for the yeast Saccharomyces cerevisiae, based on the System Dynamics approach, a general process-based model for two prokaryotic species of biotechnological interest, such as Escherichia coli and Bacillus subtilis, is proposed. The model is based on the main assumption that glycolytic intermediates act as central catabolic hub regulating the shift between respiratory and fermentative pathways. Furthermore, the description of a mixed fermentation with secondary by-products, characteristic of bacterial metabolism, is explicitly considered, parafina.
The model also represents the inhibitory effect on growth and metabolism of self-produced toxic compounds relevant in assessing the late phases of high-cell density culture. Model simulations reproduced data from experiments reported in the literature with different strains of non-recombinant and recombinant E. coli and B. subtilis cultured in both batch and fed-batch reactors. The proposed model, based on simple biological assumptions, is able to describe the main dynamics of two microbial species of relevant biotechnological interest. It demonstrates that a reductionist System Dynamics approach to formulate simplified macro-kinetic models can provide a robust representation of cell growth and accumulation in the medium of fermentation by-products.
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Developmentally-Inspired Biomimetic Culture Models to Produce Functional Islet-Like Cells From Pluripotent Precursors

Insulin-producing beta cells sourced from pluripotent stem cells hold great potential as a virtually unlimited cell source to treat diabetes. Directed pancreatic differentiation protocols aim to mimic various stimuli present during embryonic development through sequential changes of in vitro culture conditions. This is commonly accomplished by the timed addition of soluble signaling factors, in conjunction with cell-handling steps such as the formation of 3D cell aggregates. Interestingly, when stem cells at the pancreatic progenitor stage are transplanted, they form functional insulin-producing cells, suggesting that in vivo microenvironmental cues promote beta cell specification. Among these cues, biophysical stimuli have only recently emerged in the context of optimizing pancreatic differentiation protocols.
This review focuses on studies of cell-microenvironment interactions and their impact on differentiating pancreatic cells when considering cell signaling, cell-cell and cell-ECM interactions. We highlight the development of in vitro cell culture models that allow systematic studies of pancreatic cell mechanobiology in response to extracellular matrix proteins, biomechanical effects, soluble factor modulation of biomechanics, substrate stiffness, fluid flow and topography. Finally, we explore how these new mechanical insights could lead to novel pancreatic differentiation protocols that improve efficiency, maturity, and throughput.

Effects of FGFR inhibitors TKI258, BGJ398 and AZD4547 on breast cancer cells in 2D, 3D and tissue explant cultures

Purpose: Fibroblast growth factor receptors (FGFR) and pathways are important players in breast cancer (BC) development. They are commonly altered, and BCs exhibiting FGFR gene amplification are currently being studied for drug development. Here, we aimed to compare the effects of three FGFR inhibitors (FGFRis), i.e., non-selective TKI258 and selective BGJ398 and AZD4547, on different BC-derived cell lines (BCCs) and primary tissues.
Methods: The human BCCs MCF-7 and MDA-MB-231(SA) (Gentaur) and MFM223 (amplified FGFR1 and FGFR2) were analyzed for FGFR expression using qRT-PCR, and the effects of FGFRis on FGFR signaling by Western blotting. The effects of FGFRis on proliferation, viability, migration and invasion of BCCs were assessed in 2D cultures using live-cell imaging, and in 3D cultures using phenotypic analysis of organoids. To study radio-sensitization, FGFRi treatment was combined with irradiation. Patient-derived BC samples were treated with FGFRis in explant cultures and immunostained for Ki67 and cleaved caspase 3.
Results: We found that all FGFRis tested decreased the growth and viability of BC cells in 2D and 3D cultures. BGJ398 and AZD4547 were found to be potent at low concentrations in FGFR-amplified MFM233 cells, whereas higher concentrations were required in non-amplified MCF7 and MDA-MB-231(SA) cells. TKI258 inhibited the migration and invasion, whereas BGJ398 and AZD4547 only inhibited the invasion of MDA-MB-231(SA) cells. FGFRi treatment of MCF7 and MFM223 cells enhanced the inhibitory effect of radiotherapy, but this effect was not observed in MDA-MB-231(parafina) cells. FGFRi-treated primary BC explants with moderate FGFR levels showed a tendency towards decreased proliferation and increased apoptosis.
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Description: Single Donor Human Peripheral Blood Mononuclear Cells (PBMC) Frozen

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Human Peripheral Blood Mononuclear Cells, Fresh
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Description: Human Peripheral Blood Mononuclear Cells are available as positive and negative controls for T-cell monitoring in ELISPOT, ELISA, cytokine bead array, tetramer/pentamer, and flow cytometry assays. A peripheral blood mononuclear cell is defined as any blood cell with a round nucleus. These blood cells are a critical component in the immune system to fight infection and adapt to intruders. The lymphocyte population consists of CD4+ and CD8+ T cells, B cells and Natural Killer cells, CD14+ Monocytes, and Basophils/Neutrophils/Eosinophils/Dendritic cells. These cells are often extracted from whole blood or from leukopacks using ficoll, a hydrophilic polysaccharide that separates layers of blood, with monocytes and lymphocytes forming a buffy coat under a layer of plasma.Samples from each donor are tested via PCR to confirm non-reactivity.

Dog Primary Peripheral Blood Mononuclear Cells
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Human Psoriasis Peripheral Blood Mononuclear Cells
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Human PV Peripheral Blood Mononuclear Cells (Untreated)
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Human Brain Cancer Peripheral Blood Mononuclear Cells
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Normal Human Peripheral Blood Mononuclear Cells, Frozen
79059-1 30 million cells
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Description: Included Diagnoses: Adenocarcinoma (including all subtypes) and Squamous Cell Carcinoma (including all subtypes), Neuroendocrine tumors of the stomach, Carcinoma of the stomach.

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ABC-TC4267 1 vial Ask for price
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Human Type I Diabetes Peripheral Blood Mononuclear Cells
ABC-TC4263 1 vial Ask for price
Description: Peripheral blood mononuclear cells are isolated from Type I Diabetes peripheral blood by diluting the whole blood with PBS and using gradient separation techniques. After centrifugation,the mononuclear cell layer is collected. Mononuclear Cells can be processed further to isolate subpopulations. Fresh Diabetic Type I PBMCs is available upon request.

Human Type II Diabetes Peripheral Blood Mononuclear Cells
ABC-TC4265 1 vial Ask for price
Description: Human Peripheral Blood Mononuclear Cells are available as positive and negative controls for T-cell monitoring in ELISPOT, ELISA, cytokine bead array, tetramer/pentamer, and flow cytometry assays. A peripheral blood mononuclear cell is defined as any blood cell with a round nucleus. These blood cells are a critical component in the immune system to fight infection and adapt to intruders. The lymphocyte population consists of CD4+ and CD8+ T cells, B cells and Natural Killer cells, CD14+ Monocytes, and Basophils/Neutrophils/Eosinophils/Dendritic cells. These cells are often extracted from whole blood or from leukopacks using ficoll, a hydrophilic polysaccharide that separates layers of blood, with monocytes and lymphocytes forming a buffy coat under a layer of plasma.Samples from each donor are tested via PCR to confirm non-reactivity. Peripheral blood mononuclear cells are isolated from Type II Diabetes peripheral blood by diluting the whole blood with PBS and using gradient separation techniques. After centrifugation,the mononuclear cell layer is collected. Mononuclear Cells can be processed further to isolate subpopulations. Fresh Diabetic Type II PBMCs is available upon request.

Human Crohn' s Disease Peripheral Blood Mononuclear Cells
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Description: Peripheral blood mononuclear cells are isolated from Crohn's Disease peripheral blood by diluting the whole blood with PBS and using gradient separation techniques. After centrifugation,the mononuclear cell layer is collected. Mononuclear Cells can be processed further to isolate subpopulations. Fresh Crohn's PBMCs is available upon request.

Human Sickle Cell Anemia Peripheral Blood Mononuclear Cells
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Description: Peripheral blood mononuclear cells are isolated from Sickle Cell Anemia (SCA) peripheral blood by diluting the whole blood with PBS and using gradient separation techniques. After centrifugation,the mononuclear cell layer is collected. Mononuclear Cells can be processed further to isolate subpopulations. Fresh SCA PBMCs is available upon request.

Human Ankylosing Spondylitis Peripheral Blood Mononuclear Cells
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Description: Ankylosing Spondylitis is a form of arthritis that causes inflammation of the spinal joints (vertebrae), lead to severe, chronic pain. Clinical data may include: Medication (current and historical), Disease Severity.

HighQC™ Human Mobilized Peripheral Blood Mononuclear Cells
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Description: Human Peripheral Blood Mononuclear Cells (PBMC) are blood cells with a single round nucleus. These cells include lymphocytes (T, B and NK cells), monocytes and dendritic cells. Mononuclear cells are parts of the immune system which is critical for cell-mediated and humoral immunity. Mobilized peripheral blood is collected after the donor is treated with mobilization agent such as G-CSF. Such treatment mobilizes blood stem cells that are normally reside in the bone marrow to enter the blood stream. The content of CD34+ stem cells in the blood is therefore the most important parameter of graft quality. The optimal CD34+ cell dose is widely accepted to be 5 × 106/kg. Particularly, the more primitive CD34+/CD38-subset has been shown to have high self-renewal and repopulation capability. Stem cell mobilization also affects the properties of other components of the graft such as T lymphocytes and NK cells. Gentaur Human Mobilized PBMCs are isolated from healthy donors that have been mobilized with GCSF collected in acid-citrate-dextrose formula A (ACDA) as an anticoagulant. All donors must be tested negative for HBV, HCV and HIV and are IRB consented.

Human Mobilized Peripheral Blood Mononuclear Cells, CD34 Depleted
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Description: CD34 is a well-known marker for primitive and bone marrow-derived progenitor cells, especially for hematopoietic and endothelial progenitors. Gentaur offers CD34+ progenitor cells in a phenotypically undifferentiated state. The progenitor cells are derived from the peripheral blood of healthy donors that have undergone G-CSF mobilization. Progenitor cells contain two main cellular subpopulations, hematopoietic and endothelial progenitor cells. CD34+ progenitor cells are suitable for a series of studies for directed differentiation into more committed types of blood cells and endothelial lineages. Immediately after isolation, the freshly prepared CD34+ progenitor cells are cryopreserved.

Human PV Peripheral Blood Mononuclear Cells (Relapsed/Refractory)
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Description: PV Peripheral Blood Mononuclear Cells are enriched by separation using Ficoll-Paque density gradient from the peripheral blood. PV PBMCs are available in untreated and relapsed/refractory stages.

Human Chronic Kidney Disease Peripheral Blood Mononuclear Cells
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Description: Clinical data may include: Medication (current and historical), Disease Severity, Creatine Clearance Value, GFR, standard blood chemistry results, BNP.

Normal Human Peripheral Blood Mononuclear Cells, Frozen -100 million cells
79059-2 100 million cells
EUR 580
Description: Cryopreserved vial (30 x 10^6 cells) of freshly isolated primary human peripheral blood mononuclear cells (PBMCs) from a healthy donor, isolated from leukapheresis / apheresis samples using Ficoll gradient. After isolation, the PBMCs were stained to identify sub populations and evaluated for viability by flow cytometry. Cells were cryopreserved in serum-free Cryostor CS10 at a controlled rate.

Human Head and Neck Cancer Peripheral Blood Mononuclear Cells
ABC-TC4334 1 vial Ask for price
Description: Included Diagnoses: Squamous Cell Carcinoma (including all subtypes) and Adenocarcinoma (including all subtypes), Neuroendocrine tumors of the Head and Neck, Carcinoma of the Head and Neck.

Human Hepatitis B Virus (HBV) Peripheral Blood Mononuclear Cells
ABC-TC4323 1 vial Ask for price
Description: Clinical data may include: Medication (current and historical), Disease severity, Viral load, HB3Ag, HBsAg, IgM, Viral Genotype, Liver enzyme results, Co-infection presence or absence.

Human Hepatitis C Virus (HCV) Peripheral Blood Mononuclear Cells
ABC-TC4324 1 vial Ask for price
Description: Clinical data may include: Medication (current and historical), Disease severity, Viral load, Viral Genotype, Co-infection presence or absence.

Human Inflammatory Bowel Disease Peripheral Blood Mononuclear Cells
ABC-TC4332 1 vial Ask for price
Description: Clinical data may include: Medication (current and historical), Disease severity, Bristol Stool Type, Fatty Infilatration.

Human Peripheral Blood Mononuclear Cells, Single donor, Ultra-pure
ABC-TC3716 1 vial Ask for price
Description: Gentaur Mononuclear Cells (MNC) represent the enriched lymphocyte and monocyte fraction of whole blood. They are isolated from umbilical cord blood and adult peripheral blood of healthy donors at Gentaur's cell culture facility. Mononuclear Cells from cord blood contain a relatively high percentage of primitive progenitor cells,whereas Mononuclear Cells from adult peripheral blood (hMNC-PB) contain large numbers of mature immune cells.Note: When culturing pooled Peripheral Blood Mononuclear Cells, a bidirectional allogeneic mixed lymphocyte reaction (MLR) is likely to occur during the first 48 hours. This will result in cell activation, blast transformation and T-cell proliferation. Thus, these cells are preferably used for several types of infectious virus tests, including neutralization assays. For HIV-related research, prior CD8+ T-cell depletion is recommended.Freshly collected whole blood is carefully separated by optimized low-density gradient centrifugation, effectively removing the granulocytes. In order to avoid general cell damage, red blood cells are gently depleted by proprietary techniques instead of using classic osmotic lysis protocols. The obtained ultra-pure Gentaur Mononuclear Cells do not clump after thawing, and they exhibit superior viability and unchanged biological function.Immediately after isolation, the freshly prepared Mononuclear Cells are cryopreserved using Gentaur's proprietary,serum-free freezing medium, Cryo-SFM.Each cryo vial contains more than 25 million viable cells after thawing.

Human Amyotrophic Lateral Sclerosis (ALS) Peripheral Blood Mononuclear Cells
ABC-TC4344 1 vial Ask for price
Description: Amyotrophic lateral sclerosis (ALS) is a specific disease that causes the death of neurons controlling voluntary muscles.andnbsp,Clinical data may include: Medication (current and historical), Disease Severity, MMSE Score.

Human Idiopathic Pulmonary Fibrosis (IPF) Peripheral Blood Mononuclear Cells
ABC-TC4364 1 vial Ask for price
Description: Idiopathic pulmonary fibrosis is a chronic, progressive lung disease that results in scarring (fibrosis) of the lungs. Clinical data may include: Medication (current and historical), Disease severity, Lung function test results (FEV1, FEVC, PEF）.

Human Multiple Myeloma Peripheral Blood Mononuclear Cells (Relapsed/Refractory)
ABC-TC3951 1 vial Ask for price
Description: Multiple Myeloma Peripheral Blood Mononuclear Cells are isolated from Multiple Myeloma-peripheral blood by diluting the blood with phosphate-buffered salineand using gradient separation techniques. After centrifugation, the Mononuclear Cells layer is collected. Mononuclear Cells can be processed further to isolate subpopulations. Multiple Myeloma PBMCs products are available in both the newly diagnosed and the relapsed/refractory stages.

Human Waldenstrom' s Macroglobunemia Peripheral Blood Mononuclear Cells, Untreated
ABC-TC4275 1 vial Ask for price
Description: Waldenstrom's Macroglobunemia Peripheral Blood Mononuclear Cells are density separated by Ficoll-Paque to enrich mononuclear cells from peripheral blood. Waldenstrom's Macroglobunemia PBMCs are available in untreated and relapsed/refractory stages.

Human Mantle Cell Lymphoma Peripheral Blood Mononuclear Cells (Relapsed/Refactory)
ABC-TC3890 1 vial Ask for price
Description: Mantle Cell Lymphoma Peripheral Blood Mononuclear Cells are density separated by Ficoll-Paque from Mantle Cell Lymphoma peripheral blood. Mantle Cell Lymphoma PBMCs are available in untreated and relapsed/refractory stages.

Human Acute Myeloid Leukemia Peripheral Blood Mononuclear Cells (Newly Diagnosed)
ABC-TC3028 1 vial Ask for price
Description: PBMCs are isolated from Acute Myeloid Leukemia-PB by diluting the blood with phosphate-buffered saline and using gradient separation techniques. After centrifugation, the Mononuclear Cells layer is collected. Acute Myeloid Leukemia Peripheral Blood Mononuclear Cells are available in the newly diagnosed and relapsed/refractory stages.

Human Multiple Myeloma Peripheral Blood Mononuclear Cells (Newly Diagnosed/Untreated)
ABC-TC3950 1 vial Ask for price
Description: Multiple Myeloma Peripheral Blood Mononuclear Cells are isolated from Multiple Myeloma-peripheral blood by diluting the blood with phosphate-buffered salineand using gradient separation techniques. After centrifugation, the Mononuclear Cells layer is collected. Mononuclear Cells can be processed further to isolate subpopulations. Multiple Myeloma PBMCs products are available in both the newly diagnosed and the relapsed/refractory stages.

Human Acute Lymphoid Leukemia Peripheral Blood Mononuclear Cells (Relapsed/Refractory)
ABC-TC3010 1 vial Ask for price
Description: PBMCs are isolated from Acute Lymphoid Leukemia peripheral blood by diluting the whole blood with PBS and using gradient separation techniques. After centrifugation, the mononuclear cell layer is collected. Mononuclear Cells can be processed further to isolate subpopulations. Acute Lymphoid Leukemia Peripheral Blood Mononuclear Cells are available in the newly diagnosed and relapsed/refractory stages.

Human Acute Myeloid Leukemia Peripheral Blood Mononuclear Cells (Relapsed/Refractory)
ABC-TC3023 1 vial Ask for price
Description: PBMCs are isolated from Acute Myeloid Leukemia-PB by diluting the blood with phosphate-buffered saline and using gradient separation techniques. After centrifugation, the Mononuclear Cells layer is collected. Mononuclear Cells can be processed further to isolate subpopulations. Acute Myeloid Leukemia Peripheral Blood Mononuclear Cells are available in the newly diagnosed and relapsed/refractory stages.

Human Chronic Lymphoid Leukemia Peripheral Blood Mononuclear Cells (Relapsed/Refractory)
ABC-TC3349 1 vial Ask for price
Description: Chronic Lymphoid Leukemia PBMCs are isolated from Chronic Lymphoid Leukemia-peripheral blood by diluting the blood with phosphate-buffered saline and using gradient separation. Mononuclear Cells can be processed to isolate subpopulations. Chronic Lymphoid Leukemia Peripheral Blood Mononuclear Cells are available in untreated and relapsed/refractory stages.

Human Mantle Cell Lymphoma Peripheral Blood Mononuclear Cells (Newly Diagnosed/Untreated)
ABC-TC3889 1 vial Ask for price
Description: Mantle Cell Lymphoma Peripheral Blood Mononuclear Cells are density separated by Ficoll-Paque from Mantle Cell Lymphoma peripheral blood. Mantle Cell Lymphoma PBMCs are available in untreated and relapsed/refractory stages.

Human Acute Lymphoid Leukemia Peripheral Blood Mononuclear Cells (Newly Diagnosed/Untreated)
ABC-TC3009 1 vial Ask for price
Description: PBMCs are isolated from Acute Lymphoid Leukemia peripheral blood by diluting the whole blood with PBS and using gradient separation techniques. After centrifugation, the mononuclear cell layer is collected. Mononuclear Cells can be processed further to isolate subpopulations. Acute Lymphoid Leukemia Peripheral Blood Mononuclear Cells are available in the newly diagnosed and relapsed/refractory stages.

Human Chronic Myeloid Leukemia Peripheral Blood Mononuclear Cells (Accelerated/Blast Crisis)
ABC-TC3362 1 vial Ask for price
Description: Chronic Myeloid Leukemia (Chronic Myeloid Leukemia) is often divided into three phases, based on clinical characteristics and laboratory findings. In the absence of interventions, Chronic Myeloid Leukemia typically begins in the chronic phase (85% of Chronic Myeloid Leukemia patients). Over the course of several years, the cancer progresses to an accelerated phase and ultimately to a blast crisis phase.

Human Chronic Lymphoid Leukemia Peripheral Blood Mononuclear Cells (Newly Diagnosed/Untreated)
ABC-TC3348 1 vial Ask for price
Description: Chronic Lymphoid Leukemia PBMCs are isolated from Chronic Lymphoid Leukemia-peripheral blood by diluting the blood with phosphate-buffered saline and using gradient separation. Mononuclear Cells can be processed to isolate subpopulations. Chronic Lymphoid Leukemia Peripheral Blood Mononuclear Cells are available in untreated and relapsed/refractory stages.

Human Peripheral Blood Mononuclear Cell Isolation and Viability Kit
K541-30 each
EUR 451.2

Human Diffuse Large B-Cell Lymphoma Peripheral Blood Mononuclear Cells (Newly Diagnosed/Untreated)
ABC-TC3406 1 vial Ask for price
Description: Diffuse Large B-Cell Lymphoma PBMCs are density separated using Ficoll-Paque. Diffuse Large B-Cell Lymphoma Peripheral Blood Mononuclear Cells are available in the untreated and relapsed/refractory stages.

SaMag Blood DNA Extraction kit For use with SaMag-12/24 instruments; extraction of genomic DNA from whole blood, peripheral blood mononuclear cells or buffy coat
SM001 48
EUR 187.48

AnaPrep Blood DNA Extraction Kit 1200 (48) For extracting genomic DNA from mammalian whole blood, peripheral mononuclear cell, or buffy coat. Sample volume range: up to 1 ml
Z1322002 1 kit (48 extractions) Including all required plastic disposables
EUR 448.8

AnaPrep Blood DNA Extraction Kit 200 (48) For extracting genomic DNA from mammalian whole blood, peripheral mononuclear cell, or buffy coat. Sample volume range: up to 400 µl
Z1322001 1 kit (48 extractions) Including all required plastic disposables
EUR 242.68

HighQC™ Cord Blood Mononuclear Cells
ABC-SC0128T 1 vial Ask for price
Description: Human cord blood mononuclear cells are separated from umbilical cord blood by means of a density gradient centrifugation protocol. MNCs are screened for the expression CD45 and checked for viability using flow cytometry prior to cryopreservation.

Human Umbilical Cord Blood Mononuclear Cells
ABC-TC3844 1 vial Ask for price
Description: Human umbilical cord blood mononuclear cells are isolated from umbilical cord blood. Human umbilical cord blood mononuclear cells are from a single donor. These cells contain CD34+ stem cells. They enable researchers to produce various cell types of the hematopoietic lineage using standard published protocols. These cells may be used for various studies on hematopoiesis, differentiation, angiogenesis, colony formation, and surface marker expression. Presently, they are widely investigated for various therapeutic applications. Development period: Post Natal

Human Cord Blood Mononuclear Cells (CB MNC)
CBMNC-C10M 10 million
EUR 166.8

Human Cord Blood Mononuclear Cells (CB MNC)
CBMNC-C150M 150 million
EUR 1066.8

Human Cord Blood Mononuclear Cells (CB MNC)
CBMNC-C50M 50 million
EUR 718.8

Human Cord Blood Mononuclear Cells (CB MNC)
CBMNC-F100M 100 million
EUR 1438.8

Human Cord Blood Mononuclear Cells (CB MNC)
CBMNC-F200M 200 million
EUR 2038.8

Human Cord Blood Mononuclear Cells (CB MNC)
CBMNC-F50M 50 million
EUR 958.8

Human Cord Blood Mononuclear Cells CD133+ Depleted
ABC-TC3383 1 vial Ask for price
Description: Cord Blood-CD133-Depleted Mononuclear Cells are isolated by using a direct immunomagnetic CD133 MicroBead labeling system to deplete the CD133+ cells. This product contains only a minimal amount of Cord Blood-CD133+ cells.

Human Cord Blood Mononuclear Cells CD34+ Depleted, Single
ABC-TC3384 1 vial Ask for price
Description: Cord Blood CD34 Depleted Mononuclear Cells are isolated, using a direct immunomagnetic CD34 MicroBead labeling system to deplete the CD34+ cells. This product contains only a minimal amount of CD34+ cells.Cord Blood-CD34 Depleted Mononuclear Cells/Single indicates that only one donor is used to obtain the cord blood mononuclear cells.

Human Mononuclear Cells from Cord Blood, Pooled, Ultra-pure
ABC-TC3713 1 vial Ask for price
Description: Gentaur Mononuclear Cells (MNC) represent the enriched lymphocyte and monocyte fraction of whole blood. They are isolated from umbilical cord blood and adult peripheral blood of healthy donors at Gentaur's cell culture facility. Mononuclear Cells from cord blood contain a relatively high percentage of primitive progenitor cells,whereas Mononuclear Cells from adult peripheral blood (hMNC-PB) contain large numbers of mature immune cells.Note: When culturing pooled hMNC-PB, a bidirectional allogeneic mixed lymphocyte reaction (MLR) is likely to occur during the first 48 hours. This will result in cell activation, blast transformation and T-cell proliferation. Thus, these cells are preferably used for several types of infectious virus tests, including neutralization assays. For HIV-related research, prior CD8+ T-cell depletion is recommended.Freshly collected whole blood is carefully separated by optimized low-density gradient centrifugation, effectively removing the granulocytes. In order to avoid general cell damage, red blood cells are gently depleted by proprietary techniques instead of using classic osmotic lysis protocols. The obtained ultra-pure Gentaur Mononuclear Cells do not clump after thawing, and they exhibit superior viability and unchanged biological function.Immediately after isolation, the freshly prepared Mononuclear Cells are cryopreserved using Gentaur's proprietary,serum-free freezing medium, Cryo-SFM.Each cryo vial contains more than 25 million viable cells after thawing.

Human Peripheral Blood CD34+ Cells
ABC-TC4000 1 vial Ask for price
Description: NPB-CD34+ stem/progenitor cells (NPB-CD34+) are positively isolated from mononuclear cells using an immunomagnetic CD34 MicroBead labeling system.

Human Mononuclear Cells from Cord Blood, Single donor, Ultra-pure
ABC-TC3714 1 vial Ask for price
Description: Gentaur Mononuclear Cells (MNC) represent the enriched lymphocyte and monocyte fraction of whole blood. They are isolated from umbilical cord blood and adult peripheral blood of healthy donors at Gentaur's cell culture facility. Mononuclear Cells from cord blood contain a relatively high percentage of primitive progenitor cells,whereas Mononuclear Cells from adult peripheral blood (hMNC-PB) contain large numbers of mature immune cells.Note: When culturing pooled hMNC-PB, a bidirectional allogeneic mixed lymphocyte reaction (MLR) is likely to occur during the first 48 hours. This will result in cell activation, blast transformation and T-cell proliferation. Thus, these cells are preferably used for several types of infectious virus tests, including neutralization assays. For HIV-related research, prior CD8+ T-cell depletion is recommended.Freshly collected whole blood is carefully separated by optimized low-density gradient centrifugation, effectively removing the granulocytes. In order to avoid general cell damage, red blood cells are gently depleted by proprietary techniques instead of using classic osmotic lysis protocols. The obtained ultra-pure Gentaur Mononuclear Cells do not clump after thawing, and they exhibit superior viability and unchanged biological function.Immediately after isolation, the freshly prepared Mononuclear Cells are cryopreserved using Gentaur's proprietary,serum-free freezing medium, Cryo-SFM.Each cryo vial contains more than 25 million viable cells after thawing.

Human Peripheral Blood CD4+ T Cells
ABC-TC3993 1 vial Ask for price
Description: Human Peripheral Blood Mononuclear Cells are available as positive and negative controls for T-cell monitoring in ELISPOT, ELISA, cytokine bead array, tetramer/pentamer, and flow cytometry assays.

Human Peripheral Blood CD3+ T Cells
ABC-TC3994 1 vial Ask for price
Description: Human Peripheral Blood Mononuclear Cells are available as positive and negative controls for T-cell monitoring in ELISPOT, ELISA, cytokine bead array, tetramer/pentamer, and flow cytometry assays.

Human Peripheral Blood CD4+ T Cells
ABC-TC3315 1 vial Ask for price
Description: T Cells have a key function in the adaptive immune system. They can either promote growth and differentiation of other immune cells or show suppressive function and down-regulate immune reactions. These cells are often extracted from whole blood or from leukopacks using ficoll, a hydrophilic polysaccharide that separates layers of blood, with monocytes and lymphocytes forming a buffy coat under a layer of plasma. Samples from each donor are tested via PCR to confirm non-reactivity.

Human Peripheral Blood CD19+ B Cells
ABC-TC3986 1 vial Ask for price
Description: Human Peripheral Blood Mononuclear Cells are available as positive and negative controls for T-cell monitoring in ELISPOT, ELISA, cytokine bead array, tetramer/pentamer, and flow cytometry assays.

Human Peripheral Blood CD56+ NK Cells
ABC-TC3990 1 vial Ask for price
Description: Human Peripheral Blood Mononuclear Cells are available as positive and negative controls for T-cell monitoring in ELISPOT, ELISA, cytokine bead array, tetramer/pentamer, and flow cytometry assays.

Human Mobilized Peripheral Blood CD133+ Cells
ABC-TC3896 1 vial Ask for price
Description: MPB-CD133+ Stem/Progenitor cells are positively isolated from MPB-Mononuclear Cells by using a direct immunomagnetic CD133 MicroBeads labeling system.

Human Peripheral Blood CD8+ T Killer Cells
ABC-TC3991 1 vial Ask for price
Description: Human Peripheral Blood Mononuclear Cells are available as positive and negative controls for T-cell monitoring in ELISPOT, ELISA, cytokine bead array, tetramer/pentamer, and flow cytometry assays.

Human Peripheral Blood Immature Dendritic Cells
ABC-TC5467 1 vial Ask for price
Description: Primary human immature dendritic cells (DCs) were derived from immunomagnetically selected peripheral blood (PB) monocytes. Monocytes were cultured in RPMI 1640 Medium with 10% fetal bovine serum (FBS), GM-CSF and IL-4 for 5 days to generate immature DCs. PB was collected using one of the following anticoagulants: acid-citrate-dextrose (ACD), acid-citrate-dextrose solution A (ACDA), citrate-phosphate-dextrose (CPD), citrate-phosphate-double-dextrose (CP2D), or citrate-phosphate-dextrose-adenine (CPDA).

Human Normal Peripheral Blood CD56+ NK Cells
PBCD56P-C5M 5 million
EUR 825.6

Human Normal Peripheral Blood CD56+ NK Cells
PBCD56U-C5M 5 million
EUR 1014

Human Normal Peripheral Blood CD56+ NK Cells
PBCD56U-F5M 5 million
EUR 1198.8

 

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Conclusions: Our results indicate that, besides targeting FGFR-amplified BCs with selective FGFRis, also BCs without FGFR amplification/activation may benefit from FGFRi-treatment. Combination with other treatment modalities, such as radiotherapy, may allow the use of FGFRis at relatively low concentrations and, thereby, contribute to better BC treatment outcomes.

Inflammatory lncRNA AK039862 regulates paraquat-inhibited proliferation and migration of microglial and neuronal cells through the Pafah1b1/Foxa1 pathway in co-culture environments

 

Emerging evidences having suggested that particular lncRNAs have a potential effect on PD progression through provoking damage and inflammatory responses of microglia/ dopaminergic cells. In addition, paraquat can be accumulated in human body through various approaches and have an increased risk for Parkinson’s disease. However, the specific role and mechanism of lncRNA related to neurotoxic in the progression of PD is unclear. In our study, a mouse PD model was established induced by the intraperitoneal injection of paraquat (5 mg/kg and 10 mg/kg) every three days (10 times).
We determined differential expression of lncRNA AK039862 and its potential targeted genes Pafah1b1/Foxa1 in PD mouse model, then we used fluorescence in situ hybridization (FISH) to visualize the cellular distribution of AK039862. Short interfering RNAs (siRNAs) and overexpression plasmids were designed for knockdown or overexpression of AK039862. To simulate the coexisting dopaminergic cells and microglia cells in vitro, we applied several non-contact co-culture models, including conditioned medium and Transwell co-culture systems. Cytotoxicity of PQ was evaluated using bv2 cells with the concentrations: 30, 60 μM, and mn9d cells with the concentrations: 50, 100 μM. As a result, we depicted multiple interesting individual and interactive features of inflammatory lncRNA AK039862 involved in PQ-induced cellular functional effects.
First, we detected that AK039862 contributed to the neuronal injury process in PQ-treated mice and co-localization of AK039862 with dopaminergic cells in vivo. And interestingly, we demonstrated that PQ significantly inhibited microglia and dopaminergic cells proliferation and microglia migration in vitro. Further research indicated that the PQ-induced low expression of AK039862 rescued microglia proliferation and migration inhibition via the AK039862/Pafah1b1/Foxa1 pathway.
Meanwhile, AK039862 also participated in the interaction between microglia and dopaminergic cells with PQ treatment in non-contact co-culture models. In summary, we found that PQ inhibited the proliferation and migration of microglial cells, and elucidated AK039862 played a key role in PQ-induced neuroinflammatory damage through Pafah1b1/Foxa1. Finally, inflammatory AK039862 is involved in the complex communication between microglia and dopaminergic cells in the environment of PQ damage.

Human Umbilical Cord Mesenchymal Stem Cells
HUXUC-01001 1*10^6/vial
EUR 1159

Human Umbilical Cord PrimaCell™4: Normal Umbilical Vein Endothelial Cells
2-96109 1 Kit
EUR 1132.11

Human Umbilical Cord PrimaCell™1: Normal Umbilical Artery Endothelial Cells
2-96106 1 Kit
EUR 1132.11

Human Umbilical Cord PrimaCell™5: Normal Umbilical Vein Smooth Muscle Cells
2-96110 1 Kit
EUR 1132.11

Human Umbilical Cord-Derived Mesenchymal Stem Cells
cAP-0052 1Frozen Vial
EUR 594

Human Umbilical Cord-derived Mesenchymal Stem Cells
CSC-00813L One Frozen vial Ask for price

Human Umbilical Cord PrimaCell™2: Normal Umbilical Artery Smooth Muscle Cells
2-96107 1 Kit
EUR 1132.11

Human Umbilical Cord Mesenchymal Stem Cells With GFP
HUXUC-01101 1*10^6/vial
EUR 1599

Human Umbilical Cord Mesenchymal Stem Cells, 500,000 cryopreserved
SC00A1 500,000 cells
EUR 643.73

Human Umbilical Cord Tissue Preparation Buffer 4: Normal Umbilical Vein Endothelial Cells
9-80109 1 x 100 ml
EUR 160.65

Human Mesenchymal Stem Cells from Umbilical Cord Matrix
CSC-C1223 One Frozen vial Ask for price

Human Umbilical Cord Tissue Preparation Buffer 1: Normal Umbilical Artery Endothelial Cells
9-80106 1 x 100 ml
EUR 160.65

Human Umbilical Cord PrimaCell™4: Normal Umbilical Vein Endothelial Cells Growth Medium
9-46109 5 x 100 ml
EUR 151.2

Human Umbilical Cord Tissue Preparation Buffer 5: Normal Umbilical Vein Smooth Muscle Cells
9-80110 1 x 100 ml
EUR 160.65

Human Umbilical Cord PrimaCell™1: Normal Umbilical Artery Endothelial Cells Growth Medium
9-46106 5 x 100 ml
EUR 151.2

Human Umbilical Cord Tissue Preparation Buffer 2: Normal Umbilical Artery Smooth Muscle Cells
9-80107 1 x 100 ml
EUR 160.65

Human Umbilical Cord PrimaCell™5: Normal Umbilical Vein Smooth Muscle Cells Growth Medium
9-46110 5 x 100 ml
EUR 151.2

Human Primary Umbilical Cord-derived Mesenchymal Stem Cells
T5483 1x10^6 cells / 1.0 ml
EUR 1275

Rat Umbilical Cord PrimaCell™: Normal Umbilical Vein Smooth Muscle Cells
2-82587 1 Kit
EUR 1039.5

Human Umbilical Cord PrimaCell™2: Normal Umbilical Artery Smooth Muscle Cells Growth Medium
9-46107 5 x 100 ml
EUR 151.2

Mouse Umbilical Cord PrimaCell™: Normal Umbilical Vein Smooth Muscle Cells
2-82091 1 Kit
EUR 1039.5

Complete Medium For Human Umbilical Cord Mesenchymal Stem Cells
HUXUC-90011 500mL
EUR 319

RFP Expressing Human Umbilical Cord-Derived Mesenchymal Stem Cells
cAP-0052RFP 1Frozen Vial
EUR 1188

RFP Expressing Human Umbilical Cord-Derived Mesenchymal Stem Cells
RFP11 1ml frozen Vial
EUR 850

Serum Free Medium For Human Umbilical Cord Mesenchymal Stem Cells
HUXUC-90061 500mL
EUR 399

Serum, Human, Umbilical Cord
MBS638841-10mL 10mL
EUR 560

Serum, Human, Umbilical Cord
MBS638841-5x10mL 5x10mL
EUR 2375

Rat Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells
9-80294 1 x 100 ml
EUR 132.3

Umbilical Cord, Human Donor
MBS170337-INQUIRE INQUIRE Ask for price

Mouse Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells
9-80205 1 x 100 ml
EUR 160.65

Immortalized Human Umbilical Cord-Derived Mesenchymal Stem Cells-SV40T
CSC-I2041Z One Frozen vial Ask for price

Immortalized Bovine Umbilical Cord Endothelial Cells (BUcEC)
T0834 1x10^6 cells / 1.0 ml Ask for price

Rat Umbilical Cord PrimaCell™: Normal Umbilical Vein Smooth Muscle Cells Growth Medium
9-25087 5 x 100 ml
EUR 122.85

Mouse Umbilical Cord PrimaCell™: Normal Umbilical Vein Smooth Muscle Cells Growth Medium
9-32091 5 x 100 ml
EUR 122.85

Conditionally Immortalized Human Umbilical Cord-Derived Mesenchymal Stem Cells
ALIMM007 1ml frozen Vial
EUR 0.1

Conditionally Immortalized Human Umbilical Cord-Derived Mesenchymal Stem Cells
cAP-0062 1Frozen Vial
EUR 5049

Dog Primary Umbilical Cord-derived Mesenchymal Stem Cells
T5490 1x10^6 cells / 1.0 ml
EUR 1145

Dog Primary Umbilical Cord-derived Mesenchymal Stem Cells
T5491 1x10^6 cells / 1.0 ml
EUR 1145

Immortalized Human Umbilical Cord-Derived Mesenchymal Stem Cells-SV40T+hTERT
CSC-I2042Z One Frozen vial Ask for price

Single Donor Human Umbilical Cord
IRHUSCD each
EUR 957
Description: Single Donor Human Umbilical Cord

Osteogenic Differentiation Medium For Human Umbilical Cord Mesenchymal Stem Cells
HUXUC-90021 200mL
EUR 250

Adipogenic Differentiation Medium For Human Umbilical Cord Mesenchymal Stem Cells
HUXUC-90031 200+100mL
EUR 359

Plasma, Human, Umbilical Cord, Frozen
MBS634049-10mL 10mL
EUR 505

Plasma, Human, Umbilical Cord, Frozen
MBS634049-1mL 1mL
EUR 250

Plasma, Human, Umbilical Cord, Frozen
MBS634049-5x10mL 5x10mL
EUR 2060

Umbilical Cord Serum
MBS592409-10mL 10mL
EUR 355

Umbilical Cord Serum
MBS592409-50mL 50mL
EUR 1350

Chondrogenic Differentiation Medium For Human Umbilical Cord Mesenchymal Stem Cells
HUXUC-90041 100mL
EUR 409

Human Umbilical Cord PrimaCell™4: Normal Umbilical Vein Endothelial Cells Growth Supplements with Serum (for 500 ml medium)
9-47109 1 Set
EUR 321.3

Immortalized Human Umbilical Cord-Derived Mesenchymal Stem Cells-SV40 (Tet-on)
CSC-I1909Z One Frozen vial Ask for price

Human Umbilical Cord PrimaCell™1: Normal Umbilical Artery Endothelial Cells Growth Supplements with Serum (for 500 ml medium)
9-47106 1 Set
EUR 321.3

Human Umbilical Cord PrimaCell™5: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-47110 1 Set
EUR 321.3

Human Umbilical Cord PrimaCell™2: Normal Umbilical Artery Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-47107 1 Set
EUR 321.3

Human Umbilical Cord Fetal Tissue Lysate
IHUCDFSTL100UG each
EUR 870
Description: Human Umbilical Cord Fetal Tissue Lysate

Human Umbilical Cord Fetal Tissue Lysate
MBS139153-01mg 0.1mg
EUR 1145

Human Umbilical Cord Fetal Tissue Lysate
MBS139153-5x01mg 5x0.1mg
EUR 4960

Fetal Umbilical Cord Lysate
XBL-10430 0.1 mg
EUR 500.1
Description: Fetal human umbilical cord tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human umbilical cord tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the umbilical cord tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The umbilical cord tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Rat Umbilical Cord PrimaCell™: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-26087 1 Set
EUR 283.5

Mouse Umbilical Cord PrimaCell™: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-33091 1 Set
EUR 283.5

Human ; Umbilical Vein Endothelial Cells
02-701 100ng
EUR 481.2
Description: The Human ; Umbilical Vein Endothelial Cells is available in Europe and for worldwide shipping via Gentaur.

Human Umbilical Vein Endothelial Cells
ABC-TC3848 1 vial Ask for price
Description: HUVEC are isolated from the vein of the umbilical cord and are commonly used for physiological and pharmacological investigations, such as macromolecule transport, blood coagulation, angiogenesis, and fibrinolysis. Human umbilical vein endothelial cells have played a major role as a model system for the study of the regulation of endothelial cell function and the role of the endothelium in the response of the blood vessel wall to stretch, shear forces, and the development of atherosclerotic plaques.Each human umbilical cord vein is individually processed to isolate endothelial cells through collagenase digestion and culture. Frozen HUVEC products are cryopreserved at the end of the primary culture. HUVEC are guaranteed to reach 15 population doublings when cultured with Gentaur's recommended medium.Samples from each donor are tested via PCR to confirm non-reactivity.

Human Umbilical Vein Endothelial Cells 
CSC-C1578 One Frozen vial Ask for price

Human Umbilical Vein Endothelial Cells
HEC01 500,000+ Cells frozen
EUR 387.72

Human Umbilical Vein Endothelial Cells
HUVEC-20001 1*10^6/vial
EUR 1100

Human Umbilical Vein Endothelial Cells
MBS556003-INQUIRE INQUIRE Ask for price

Human Umbilical Artery Endothelial Cells
ABC-TC3841 1 vial Ask for price
Description: Gentaur endothelial cells from large vessels are available from different locations, e.g. umbilical vein, umbilical artery, aorta, coronary artery, pulmonary artery, and saphenous vein. In addition, Gentaur offers HUVEC isolated in standard Endothelial Cell Growth Medium and also in Endothelial Cell Growth Medium 2. The cells are supplied either from single donors or from pooled donors (from up to four different umbilical cords). Furthermore, HUVEC are available which are prescreened for VEGF (vascular endothelial cell growth factor) response. Shortly after isolation, Gentaur Human Endothelial Cells from large vessels are cryopreserved at passage 1 or passage 2 (see page 5) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

Human Umbilical Vein Smooth Muscle Cells
ABC-TC3857 1 vial Ask for price
Description: Vascular smooth muscle cell is the cellular substate of most significant arterial disease. The increased growth potential of vascular smooth muscle cells represents one of the crucial anomalies responsible for the development of essential vascular diseases. New studies demonstrate that vascular smooth muscle cells express ICAM-1 and VCAM-1, which may contribute to the inflammatory reaction in the vascular wall and may actively be involved in the progression and stability of vascular disease. In vitro culture of human VSMC as a model of vascular research played a critical role and continues providing information in the pharmacology and the therapy of vascular diseases.HUVSMC from Gentaur Research Laboratories are isolated from human umbilical veins. HUVSMC are cryopreserved at the end of secondary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HUVSMC are characterized by immunofluorescent method with antibodies to ?-smooth muscle actin and desmin. HUVSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HUVSMC are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Human Umbilical Artery Smooth Muscle Cells
ABC-TC3843 1 vial Ask for price
Description: Smooth muscle tissue is found in the tunica media layer of large and small blood vessels and in the walls of hollow organs like the bladder and the uterus. Smooth Muscle Cells (SMC) possess all the same types of filaments, but, depending on the tissue of origin, differ significantly in mechanical and physiological properties. Therefore, Gentaur offers a range of Human Smooth Muscle Cells produced at Gentaur's cell culture facility from normal human tissue of different origins. The cells are isolated from the aorta, the coronary artery, the pulmonary artery, the umbilical artery,the trachea, the bronchi, the bladder,the prostate, and the uterus. Shortly after isolation, all Gentaur Human Smooth Muscle Cells are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

Human Cord Blood CD19+ B Cells
CBCD19-C2.5M 2.5 million
EUR 906

Human Cord Blood CD19+ B Cells
CBCD19-C5M 5 million
EUR 1078.8

Human Cord Blood CD19+ B Cells
CBCD19-F5M 5 million
EUR 1198.8

Human Cord Blood CD19+ B Cells
ABC-TC3368 1 vial Ask for price
Description: Cord Blood-CD19+ B cells are positively isolated from mononuclear cells, using a direct immunomagnetic CD19 MicroBeads labeling system

Human Umbilical Cord OptiTDS™1: Tissue Dissociation System
4-28106 1 Kit
EUR 274.05

Human Umbilical Cord OptiTDS™2: Tissue Dissociation System
4-28107 1 Kit
EUR 274.05

Human Umbilical Cord OptiTDS™4: Tissue Dissociation System
4-28109 1 Kit
EUR 274.05

Human Umbilical Cord OptiTDS™5: Tissue Dissociation System
4-28110 1 Kit
EUR 274.05

OCPB00610-100UG - Fetal Umbilical Cord Lysate
OCPB00610-100UG 0.1mg
EUR 439

Human Umbilical Vein Endothelial Cells (HUVECs)
cAP-0001 1Frozen Vial
EUR 217.8

Human Umbilical Vein Endothelial Cells, Pooled
ABC-TC3849 1 vial Ask for price
Description: The cells are cryopreserved in the second passage to ensure the highest viability and plating efficiency. Primary pooled HUVECs are derived from 10 individual donors, minimizing the lot-to-lot variability associated with cells derived from single donors.

Human Umbilical Artery Endothelial Cells (HUAECs)
cAP-0113 1Frozen Vial
EUR 396

Human Umbilical Vein Endothelial Cells, Neonatal
ABC-TC3853 1 vial Ask for price
Description: Human Umbilical Vein Endothelial Cells (HUVEC) are isolated from normal human umbilical vein. They are cryopreserved at the end of primary culture and can be cultured and propagated at least 16 population doublings.

Human Primary Umbilical Vein Endothelial Cells
T4098 5x10^5 cells / 1.0 ml Ask for price

Human Primary Umbilical Vein Endothelial Cells
T5482 1x10^6 cells / 1.0 ml
EUR 765

Human Umbilical Artery Endothelial Cells, Neonatal
ABC-TC3842 1 vial Ask for price
Description: Human Umbilical Artery Endothelial Cells (HUAEC) are isolated from normal human umbilical arteries. They are cryopreserved at second passage and can be cultured and propagated at least 15 population doublings.

Human Primary Umbilical Artery Endothelial Cells
T4099 5x10^5 cells / 1.0 ml Ask for price

HighQC™ Human Mesenchymal Stem Cell (Umbilical Cord Matrix/ Cell Pellet In RNAlater)
ABC-SC0038T 1 vial Ask for price
Description: Mesenchymal Stem Cells (MSC), also termed Mesenchymal Stromal Cells, are multipotent cells that can differentiate into a variety of cell types and have the capacity for self renewal. MSC have been shown to differentiate in vitro or in vivo into adipocytes, chondrocytes, osteoblasts, myocytes, neurons, hepatocytes, and pancreatic islet cells. Optimized Gentaur media are available to support both the growth of MSC and their differentiation into several different lineages. Recent experiments suggest that differentiation capabilities into diverse cell types vary between MSC of different origin.Gentaur hMSC are harvested from normal human adipose tissue, bone marrow, and umbilical cord matrix (Wharton's jelly) of individual donors.The cells are tested for their ability to differentiate in vitro into adipocytes, chondrocytes, and osteoblasts. Our hMSC show a verified marker expression profile that complies with ISCT* recommendations, providing well characterized cells.

HighQC™ RFP-Human Umbilical Cord-Derived Mesenchymal Stem Cell
ABC-FC0048 1 vial Ask for price
Description: HUcMSCs are isolated from umbilical cord matrix (Wharton's Jelly) and demonstrated with spindle-shaped-, and fibroblast-like cells. RFP-HUcMSCs are puromycin resistant HUcMSCs after trandsfected with lentiviruses expressing RFP under the control of CMV protomer. Each lot of RFP-HUcMSCs is tested to ensure the cells can be passaged at least three times (i.e., approximately 9 to 10 population doublings) after thaw in complete growth media (Mesenchymal Stem Cell Growth Medium (MSCGM)), when cultured following the detailed protocol described below.

Innovative Grade US Origin Bovine Umbilical Cord
IGBOCDF each
EUR 210
Description: Innovative Grade US Origin Bovine Umbilical Cord
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