Cell Cultures for Virology: Usability, Advantages, and Prospects
Virus detection in natural and clinical samples is a complicated problem in research and diagnostics. There are different approaches for virus isolation and identification, including PCR, CRISPR/Cas technology, NGS, immunoassays, and cell-based assays. Following the development of genetic engineering methods, approaches that utilize cell cultures have become useful and informative. Molecular biology methods allow increases in the sensitivity and specificity of cell cultures for certain viruses and can be used to generate reporter cell lines.
These cell lines express specific reporter proteins (e.g., GFP, luciferase, and CAT) in response to virus infection that can be detected in a laboratory setting. The development of genome editing and synthetic biology methods has given rise to new perspectives regarding the design of virus reporter systems in cell cultures. This review is aimed at describing both virology methods in general and examples of the development of cell-based methods that exist today.
A General Process-Based Model for Describing the Metabolic Shift in Microbial Cell Cultures
The metabolic shift between respiration and fermentation at high glucose concentration is a widespread phenomenon in microbial world, and it is relevant for the biotechnological exploitation of microbial cell factories, affecting the achievement of high-cell-densities in bioreactors. Starting from a model already developed for the yeast Saccharomyces cerevisiae, based on the System Dynamics approach, a general process-based model for two prokaryotic species of biotechnological interest, such as Escherichia coli and Bacillus subtilis, is proposed. The model is based on the main assumption that glycolytic intermediates act as central catabolic hub regulating the shift between respiratory and fermentative pathways. Furthermore, the description of a mixed fermentation with secondary by-products, characteristic of bacterial metabolism, is explicitly considered.
The model also represents the inhibitory effect on growth and metabolism of self-produced toxic compounds relevant in assessing the late phases of high-cell density culture. Model simulations reproduced data from experiments reported in the literature with different strains of non-recombinant and recombinant E. coli and B. subtilis cultured in both batch and fed-batch reactors. The proposed model, based on simple biological assumptions, is able to describe the main dynamics of two microbial species of relevant biotechnological interest. It demonstrates that a reductionist System Dynamics approach to formulate simplified macro-kinetic models can provide a robust representation of cell growth and accumulation in the medium of fermentation by-products.
Developmentally-Inspired Biomimetic Culture Models to Produce Functional Islet-Like Cells From Pluripotent Precursors
Insulin-producing beta cells sourced from pluripotent stem cells hold great potential as a virtually unlimited cell source to treat diabetes. Directed pancreatic differentiation protocols aim to mimic various stimuli present during embryonic development through sequential changes of in vitro culture conditions. This is commonly accomplished by the timed addition of soluble signaling factors, in conjunction with cell-handling steps such as the formation of 3D cell aggregates. Interestingly, when stem cells at the pancreatic progenitor stage are transplanted, they form functional insulin-producing cells, suggesting that in vivo microenvironmental cues promote beta cell specification. Among these cues, biophysical stimuli have only recently emerged in the context of optimizing pancreatic differentiation protocols.
This review focuses on studies of cell-microenvironment interactions and their impact on differentiating pancreatic cells when considering cell signaling, cell-cell and cell-ECM interactions. We highlight the development of in vitro cell culture models that allow systematic studies of pancreatic cell mechanobiology in response to extracellular matrix proteins, biomechanical effects, soluble factor modulation of biomechanics, substrate stiffness, fluid flow and topography. Finally, we explore how these new mechanical insights could lead to novel pancreatic differentiation protocols that improve efficiency, maturity, and throughput.
Effects of FGFR inhibitors TKI258, BGJ398 and AZD4547 on breast cancer cells in 2D, 3D and tissue explant cultures
Purpose: Fibroblast growth factor receptors (FGFR) and pathways are important players in breast cancer (BC) development. They are commonly altered, and BCs exhibiting FGFR gene amplification are currently being studied for drug development. Here, we aimed to compare the effects of three FGFR inhibitors (FGFRis), i.e., non-selective TKI258 and selective BGJ398 and AZD4547, on different BC-derived cell lines (BCCs) and primary tissues.
Methods: The human BCCs MCF-7 and MDA-MB-231(SA) (wild-type FGFR) and MFM223 (amplified FGFR1 and FGFR2) were analyzed for FGFR expression using qRT-PCR, and the effects of FGFRis on FGFR signaling by Western blotting. The effects of FGFRis on proliferation, viability, migration and invasion of BCCs were assessed in 2D cultures using live-cell imaging, and in 3D cultures using phenotypic analysis of organoids. To study radio-sensitization, FGFRi treatment was combined with irradiation. Patient-derived BC samples were treated with FGFRis in explant cultures and immunostained for Ki67 and cleaved caspase 3.
Results: We found that all FGFRis tested decreased the growth and viability of BC cells in 2D and 3D cultures. BGJ398 and AZD4547 were found to be potent at low concentrations in FGFR-amplified MFM233 cells, whereas higher concentrations were required in non-amplified MCF7 and MDA-MB-231(SA) cells. TKI258 inhibited the migration and invasion, whereas BGJ398 and AZD4547 only inhibited the invasion of MDA-MB-231(SA) cells. FGFRi treatment of MCF7 and MFM223 cells enhanced the inhibitory effect of radiotherapy, but this effect was not observed in MDA-MB-231(SA) cells. FGFRi-treated primary BC explants with moderate FGFR levels showed a tendency towards decreased proliferation and increased apoptosis.

eaaci2015
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh |
BMC-F500M |
101Bio |
- |
Ask for price |
Human Peripheral Blood Mononuclear Cell Isolation and Viability Kit |
K541-30 |
Biovision |
|
EUR 376 |
Human Peripheral Blood CD3+Â T Cells, Cryopreserved |
PBCD3-C10M |
101Bio |
- |
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Human Peripheral Blood CD3+Â T Cells, Cryopreserved |
PBCD3-C20M |
101Bio |
- |
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Human Peripheral Blood CD3+Â T Cells, Fresh |
PBCD3-F20M |
101Bio |
- |
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Positively Selected Normal Peripheral Blood CD19+ B Cells, Cryopreserved |
PBCD19P-C10M |
101Bio |
- |
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Positively Selected Normal Peripheral Blood CD19+ B Cells, Fresh |
PBCD19P-F10M |
101Bio |
- |
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Immortalized Human Bone Marrow Mononuclear Cells |
T0526 |
ABM |
1x106 cells / 1.0 ml |
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Peripheral Blood Leukocyte (PBL's) Lysate |
1371 |
ProSci |
0.1 mg |
EUR 191 |
Description: Peripheral Blood Leukocyte (PBL's) tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Peripheral Blood Leukocyte Membrane Lysate |
XBL-10813 |
ProSci |
0.1 mg |
EUR 737 |
Description: Human peripheral blood leukocyte tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human peripheral blood leukocyte tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated peripheral blood leukocyte tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated peripheral blood leukocyte tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
Human Normal Peripheral Blood CD4+/CD25+ Regulatory T Cells (T reg), Cryopreserved |
PBCD4-25-C2M |
101Bio |
- |
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Human Normal Peripheral Blood CD4+/CD25+ Regulatory T Cells (T reg), Fresh |
PBCD4-25-F2M |
101Bio |
- |
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Sheep Red Blood Cells |
20R-RS001 |
Fitzgerald |
10 ml |
EUR 521 |
Description: Human IgG Senstitized Gluteraldehyde Stabilized Sheep Red Bllood Cells |
Bovine Red Blood Cells |
88R-B002 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Bovine Red Blood Cells |
Chicken Red Blood Cells |
88R-C002 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Chicken Red Blood Cells |
Dog Red Blood Cells |
88R-D004 |
Fitzgerald |
10 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Dog Red Blood Cells |
Goat Red Blood Cells |
88R-G003 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Goat Red Blood Cells |
Hamster Red Blood Cells |
88R-H001 |
Fitzgerald |
5 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Hamster Red Blood Cells |
Horse Red Blood Cells |
88R-H002 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Horse Red Blood Cells |
Hamster Red Blood Cells |
88R-H003 |
Fitzgerald |
10 ml |
EUR 489 |
Description: Washed and freshly prepared 10% suspension of Hamster Red Blood Cells |
Mouse Red Blood Cells |
88R-M001 |
Fitzgerald |
10 ml |
EUR 295 |
Description: Washed and freshly prepared 10% suspension of Mouse Red Blood Cells |
Monkey Red Blood Cells |
88R-M003 |
Fitzgerald |
5 x 2 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Monkey Red Blood Cells |
Mouse Red Blood Cells |
88R-M004 |
Fitzgerald |
5 x 2 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Mouse Red Blood Cells |
Pig Red Blood Cells |
88R-P002 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Pig Red Blood Cells |
Rabbit Red Blood Cells |
88R-R001 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Rabbit Red Blood Cells |
Rat Red Blood Cells |
88R-R002 |
Fitzgerald |
5 x 2.0ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Rat Red Blood Cells |
Rat Red Blood Cells |
88R-R005 |
Fitzgerald |
25 ml |
EUR 327 |
Description: Washed and freshly prepared 10% suspension of Rat Red Blood Cells |
Sheep Red Blood Cells |
88R-S001 |
Fitzgerald |
100 ml |
EUR 512 |
Description: Washed and freshly prepared 10% suspension of Sheep Red Blood Cells |
Sheep Red Blood Cells |
88R-S003 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Sheep Red Blood Cells |
Sheep Red Blood Cells |
88R-S004 |
Fitzgerald |
10 ml |
EUR 521 |
Description: Glutaraldehyde-stabilized freshly prepared albumin sensitised Sheep Red Blood Cells |
Sheep Red Blood Cells |
88R-S005 |
Fitzgerald |
10 ml |
EUR 446 |
Description: Glutaraldehyde-stabilized freshly prepared tanned Sheep Red Blood Cells |
Turkey Red Blood Cells |
88R-T001 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Turkey Red Blood Cells |
Human Peripheral blood leukocyte (PBL's, WBC) lysate |
HCL-1371 |
Alpha Diagnostics |
100ug |
EUR 238 |
Rabbit Red Blood Cells (100%) |
20-abx800015 |
Abbexa |
|
|
|
Sheep Red Blood Cells (100%) |
20-abx800032 |
Abbexa |
|
|
|
Goat Red Blood Cells (100%) |
20-abx800047 |
Abbexa |
|
|
|
Horse Red Blood Cells (100%) |
20-abx800062 |
Abbexa |
-
EUR 453.00
-
EUR 551.00
-
EUR 384.00
|
|
|
Horse Red Blood Cells (100%) |
abx800062-1L |
Abbexa |
1 L |
EUR 746 |
|
Donkey Red Blood Cells (100%) |
20-abx800077 |
Abbexa |
-
EUR 676.00
-
EUR 843.00
-
EUR 481.00
|
|
|
Donkey Red Blood Cells (100%) |
abx800077-1L |
Abbexa |
1 L |
EUR 1191 |
|
Camel Red Blood Cells (100%) |
20-abx800092 |
Abbexa |
-
EUR 746.00
-
EUR 1636.00
-
EUR 578.00
|
|
|
Camel Red Blood Cells (100%) |
abx800092-1L |
Abbexa |
1 L |
EUR 3042 |
|
Dog Red Blood Cells (100%) |
20-abx800122 |
Abbexa |
|
|
|
Chicken Red Blood Cells (100%) |
20-abx800137 |
Abbexa |
|
|
|
Guinea Pig Red Blood Cells |
88R-1047 |
Fitzgerald |
15 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Guinea Pig Red Blood Cells |
Guinea Pig Red Blood Cells |
88R-G002 |
Fitzgerald |
25 ml |
EUR 365 |
Description: Washed and freshly prepared 10% suspension of Guinea Pig Red Blood Cells |
Guinea Pig Red Blood Cells |
88R-P001 |
Fitzgerald |
15 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Guinea Pig Red Blood Cells |
Human Cord Blood CD34+ Cells Derived Endothelial Cells |
HEC23 |
Neuromics |
500,000+ Cells |
EUR 1080 |
Neural Stem Cells (iPSC from Blood Cells; Male) |
ASE-9234 |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 414.5 |
Description: 12 month |
Neural Stem Cells (iPSC from Blood Cells; Female) |
ASE-9234F |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 414.5 |
Description: 12 month |
cDNA from Human Adult Normal Tissue: Peripheral Blood Leukocyte |
C1234148-10 |
Biochain |
10 reactions |
EUR 231 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Beagle dog Red Blood Cells (100%) |
20-abx800107 |
Abbexa |
|
|
|
Rabbit RED BLOOD CELLS HEPARIN 500mL* |
31159-5 |
Pel-Freez |
500mL |
EUR 821.9 |
Neurons (iPSC from Blood Cells; Male) |
ASE-9321 |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 313.25 |
Description: 12 month |
Neurons (iPSC from Blood Cells; Female) |
ASE-9321F |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 313.25 |
Description: 12 month |
IMEMS Random Nanofibers 6 Chambers |
3D00011 |
Neuromics |
700 nm-random PCL inserts |
EUR 96 |
IMEMS Aligned Nanofibers 6 Chambers |
3D00017 |
Neuromics |
2 750 nm-aligned PCL inserts |
EUR 440 |
Methylated & Low-Methylated DNA Matching Pair - Human Peripheral Blood Leukocyte |
D6234148-PP |
Biochain |
2x5 ug |
EUR 343 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Total Protein from Human Adult Normal Tissue: Peripheral Blood Leukocyte |
P1234148 |
Biochain |
1 mg |
EUR 356 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
Membrane Protein from Human Adult Normal Tissue: Peripheral Blood Leukocyte |
P3234148 |
Biochain |
0.1 mg |
EUR 516 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
Total RNA from Human Adult Normal Tissue: Peripheral Blood Leukocyte |
R1234148-10 |
Biochain |
10 ug |
EUR 194 |
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Rat Anti Rat Rt6.1 (Peripheral T Cells) Monoclonal Antibody |
CABT-49825RR |
Creative Diagnostics |
2 ml |
EUR 533 |
Astrocytes Mature (iPSC from Blood Cells; Male) |
ASE-9322M |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 455 |
Description: 12 month |
Astrocytes Mature (iPSC from Blood Cells; Female) |
ASE-9322MF |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 455 |
Description: 12 month |
Astrocytes Precursors (iPSC from Blood Cells; Male) |
ASE-9322P |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 374 |
Description: 12 month |
Astrocytes Precursors (iPSC from Blood Cells; Female) |
ASE-9322PF |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 374 |
Description: 12 month |
Dopaminergic Neurons (iPSC from Blood Cells; Male) |
ASE-9323 |
Applied StemCell |
1 vial |
EUR 374 |
Description: 12 month |
Dopaminergic Neurons (iPSC from Blood Cells; Female) |
ASE-9323F |
Applied StemCell |
1 vial |
EUR 374 |
Description: 12 month |
Human  Peripheral  Blood  CD14+ Monocytes, Cryopreserved / Untouched |
PBCD14U-C10M |
101Bio |
- |
Ask for price |
Human  Peripheral  Blood  CD14+ Monocytes, Fresh / Untouched |
PBCD14U-F10M |
101Bio |
- |
Ask for price |
96 Well Human Adult Normal Genomic DNA Plate - Peripheral Blood Leukocyte |
D8234148-1 |
Biochain |
1 plate |
EUR 256 |
Human Cord Blood CD34+ Cells, Cryopreserved, single donor |
CBCD34-C0.5M |
101Bio |
- |
Ask for price |
Human Cord Blood CD34+ Cells, Cryopreserved, single donor |
CBCD34-C1M |
101Bio |
- |
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Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor |
CBCD34mix-C0.5M |
101Bio |
- |
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Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor |
CBCD34mix-C1M |
101Bio |
- |
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Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor |
CBCD34mix-C5M |
101Bio |
- |
Ask for price |
Neurons Starter Kit (iPSC from Blood Cells; Male) |
ASE-9321K |
Applied StemCell |
1 Kit |
EUR 394.25 |
Description: 12 month |
Neurons Starter Kit (iPSC from Blood Cells; Female) |
ASE-9321KF |
Applied StemCell |
1 Kit |
EUR 394.25 |
Description: 12 month |
Human  Peripheral  Blood  CD14+ Monocytes, Cryopreserved / Positive selection |
PBCD14P-C10M |
101Bio |
- |
Ask for price |
Human  Peripheral  Blood  CD14+ Monocytes, Fresh / Positive selection |
PBCD14P-F10M |
101Bio |
- |
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Mouse Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells |
2-82072 |
CHI Scientific |
1 Kit |
Ask for price |
Rat Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells |
2-82571 |
CHI Scientific |
1 Kit |
Ask for price |
Human Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells |
2-96088 |
CHI Scientific |
1 Kit |
Ask for price |
Astrocytes Precursors Starter Kit (iPSC from Blood Cells; Male) |
ASE-9322PK |
Applied StemCell |
1 Kit |
EUR 414.5 |
Description: 12 month |
Astrocytes Precursors Starter Kit (iPSC from Blood Cells; Female) |
ASE-9322PKF |
Applied StemCell |
1 Kit |
EUR 414.5 |
Description: 12 month |
Dopaminergic Neurons Starter Kit (iPSC from Blood Cells; Male) |
ASE-9323K |
Applied StemCell |
1 vial |
EUR 455 |
Description: 12 month |
Dopaminergic Neurons Starter Kit (iPSC from Blood Cells; Female) |
ASE-9323KF |
Applied StemCell |
1 vial |
EUR 455 |
Description: 12 month |
Human  Peripheral  Blood  CD4+  T  Lymphocytes, Cryopreserved |
PBCD4-C10M |
101Bio |
- |
Ask for price |
Human  Peripheral  Blood  CD4+  T  Lymphocytes, Fresh |
PBCD4-F10M |
101Bio |
- |
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Human  Peripheral  Blood  CD4+  T  Lymphocytes, Fresh |
PBCD4-F20M |
101Bio |
- |
Ask for price |
Anti-CD3/CD28 stimulus for human PBMC |
CT372 |
U-CyTech |
50 tests |
EUR 118 |
Genomic DNA from Human Adult Normal Tissue: Peripheral Blood Leukocyte, from a single donor |
D1234148 |
Biochain |
100 ug |
EUR 282 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Conclusions: Our results indicate that, besides targeting FGFR-amplified BCs with selective FGFRis, also BCs without FGFR amplification/activation may benefit from FGFRi-treatment. Combination with other treatment modalities, such as radiotherapy, may allow the use of FGFRis at relatively low concentrations and, thereby, contribute to better BC treatment outcomes.
Inflammatory lncRNA AK039862 regulates paraquat-inhibited proliferation and migration of microglial and neuronal cells through the Pafah1b1/Foxa1 pathway in co-culture environments
Emerging evidences having suggested that particular lncRNAs have a potential effect on PD progression through provoking damage and inflammatory responses of microglia/ dopaminergic cells. In addition, paraquat can be accumulated in human body through various approaches and have an increased risk for Parkinson’s disease. However, the specific role and mechanism of lncRNA related to neurotoxic in the progression of PD is unclear. In our study, a mouse PD model was established induced by the intraperitoneal injection of paraquat (5 mg/kg and 10 mg/kg) every three days (10 times).
We determined differential expression of lncRNA AK039862 and its potential targeted genes Pafah1b1/Foxa1 in PD mouse model, then we used fluorescence in situ hybridization (FISH) to visualize the cellular distribution of AK039862. Short interfering RNAs (siRNAs) and overexpression plasmids were designed for knockdown or overexpression of AK039862. To simulate the coexisting dopaminergic cells and microglia cells in vitro, we applied several non-contact co-culture models, including conditioned medium and Transwell co-culture systems. Cytotoxicity of PQ was evaluated using bv2 cells with the concentrations: 30, 60 μM, and mn9d cells with the concentrations: 50, 100 μM. As a result, we depicted multiple interesting individual and interactive features of inflammatory lncRNA AK039862 involved in PQ-induced cellular functional effects.
First, we detected that AK039862 contributed to the neuronal injury process in PQ-treated mice and co-localization of AK039862 with dopaminergic cells in vivo. And interestingly, we demonstrated that PQ significantly inhibited microglia and dopaminergic cells proliferation and microglia migration in vitro. Further research indicated that the PQ-induced low expression of AK039862 rescued microglia proliferation and migration inhibition via the AK039862/Pafah1b1/Foxa1 pathway.
Meanwhile, AK039862 also participated in the interaction between microglia and dopaminergic cells with PQ treatment in non-contact co-culture models. In summary, we found that PQ inhibited the proliferation and migration of microglial cells, and elucidated AK039862 played a key role in PQ-induced neuroinflammatory damage through Pafah1b1/Foxa1. Finally, inflammatory AK039862 is involved in the complex communication between microglia and dopaminergic cells in the environment of PQ damage.
Human Cord Blood CD34+ Cells Derived Endothelial Cells |
HEC23 |
Neuromics |
500,000+ Cells |
EUR 1080 |
Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells Growth Medium |
9-46107 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells Growth Medium |
9-46110 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Human Umbilical Cord Tissue Preparation Buffer 1: Normal Umbilical Artery Endothelial Cells |
9-80106 |
CHI Scientific |
1 x 100 ml |
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Human Umbilical Cord Tissue Preparation Buffer 4: Normal Umbilical Vein Endothelial Cells |
9-80109 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Fetal Umbilical Cord Lysate |
XBL-10430 |
ProSci |
0.1 mg |
EUR 416.75 |
Description: Fetal human umbilical cord tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human umbilical cord tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the umbilical cord tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The umbilical cord tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Medium |
9-25087 |
CHI Scientific |
5 x 100 ml |
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Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Medium |
9-32091 |
CHI Scientific |
5 x 100 ml |
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Mouse Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells |
9-80205 |
CHI Scientific |
1 x 100 ml |
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Rat Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells |
9-80294 |
CHI Scientific |
1 x 100 ml |
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Human Umbilical Cord Tissue Preparation Buffer 2: Normal Umbilical Artery Smooth Muscle Cells |
9-80107 |
CHI Scientific |
1 x 100 ml |
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Human Umbilical Cord Tissue Preparation Buffer 5: Normal Umbilical Vein Smooth Muscle Cells |
9-80110 |
CHI Scientific |
1 x 100 ml |
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Human Cord Blood CD34+ Cells, Cryopreserved, single donor |
CBCD34-C0.5M |
101Bio |
- |
Ask for price |
Human Cord Blood CD34+ Cells, Cryopreserved, single donor |
CBCD34-C1M |
101Bio |
- |
Ask for price |
Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor |
CBCD34mix-C0.5M |
101Bio |
- |
Ask for price |
Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor |
CBCD34mix-C1M |
101Bio |
- |
Ask for price |
Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor |
CBCD34mix-C5M |
101Bio |
- |
Ask for price |
Positively Selected Normal Peripheral Blood CD19+ B Cells, Cryopreserved |
PBCD19P-C10M |
101Bio |
- |
Ask for price |
Positively Selected Normal Peripheral Blood CD19+ B Cells, Fresh |
PBCD19P-F10M |
101Bio |
- |
Ask for price |
human umbilical vein endothelial cells, Peptide Aptamer, Biotinylated |
AP-316-B |
Alpha Diagnostics |
1 mg |
Ask for price |
Human Umbilical Cord OptiTDS1: Tissue Dissociation System |
4-28106 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord OptiTDS2: Tissue Dissociation System |
4-28107 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord OptiTDS4: Tissue Dissociation System |
4-28109 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord OptiTDS5: Tissue Dissociation System |
4-28110 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord PrimaCell1: Normal Umbilical Artery Endothelial Cells Growth Supplements with Serum (for 500 ml medium) |
9-47106 |
CHI Scientific |
1 Set |
Ask for price |
Human Umbilical Cord PrimaCell4: Normal Umbilical Vein Endothelial Cells Growth Supplements with Serum (for 500 ml medium) |
9-47109 |
CHI Scientific |
1 Set |
Ask for price |
Human ; Umbilical Vein Endothelial Cells |
02-701 |
Sceti |
100ng |
EUR 401 |
Description: The Human ; Umbilical Vein Endothelial Cells is available in Europe and for worldwide shipping via Gentaur. |
Human Umbilical Vein Endothelial Cells |
HEC01 |
Neuromics |
500,000+ Cells frozen |
EUR 440 |
Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium) |
9-47107 |
CHI Scientific |
1 Set |
Ask for price |
Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium) |
9-47110 |
CHI Scientific |
1 Set |
Ask for price |
Mouse Umbilical Cord OptiTDS: Tissue Dissociation System |
4-28205 |
CHI Scientific |
1 Kit |
Ask for price |
Rat Umbilical Cord OptiTDS: Tissue Dissociation System |
4-28294 |
CHI Scientific |
1 Kit |
Ask for price |
VEGF-stimulated human umbilical vein endothelial cells, Peptide Aptamer, Biotinylated |
AP-339-B |
Alpha Diagnostics |
1 mg |
Ask for price |
Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium) |
9-26087 |
CHI Scientific |
1 Set |
Ask for price |
Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium) |
9-33091 |
CHI Scientific |
1 Set |
Ask for price |
Cancer Antigen AFP Antigen (Human Cord Blood) |
VAng-Wyb8624-inquire |
Creative Biolabs |
inquire |
Ask for price |
Description: Alpha Fetoprotein (66 kDa), Cancer antigen from Human Cord Blood, 1.73 mg/mL. |
Human  Cord  Blood  CD34+  Cells (Mixed  Donors) |
CBCD34M-05 |
101Bio |
- |
Ask for price |
Human  Cord  Blood  CD34+  Cells (Mixed  Donors) |
CBCD34M-1 |
101Bio |
- |
Ask for price |
Human  Cord  Blood  CD34+  Cells (Mixed  Donors) |
CBCD34M-5 |
101Bio |
- |
Ask for price |
GFP Expressing Human Umbilical Vein Endothelial Cells |
GF01 |
Neuromics |
500,000+ Cells frozen |
EUR 1040 |
Immortalized Human Umbilical Vein Endothelial Cells - SV40 |
T0014 |
ABM |
1x106 cells / 1.0 ml |
Ask for price |
Human Umbilical Cord FibrOut1: Fibroblast Inhibitory System (for 500 ml medium) |
7-15095 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord FibrOut2: Fibroblast Inhibitory System (for 500 ml medium) |
7-15096 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord FibrOut4: Fibroblast Inhibitory System (for 500 ml medium) |
7-15098 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord FibrOut5: Fibroblast Inhibitory System (for 500 ml medium) |
7-15099 |
CHI Scientific |
1 Kit |
Ask for price |
Human B-lymphocyte antigen CD19 (CD19) |
1-CSB-CF004888HU |
Cusabio |
-
EUR 1298.00
-
EUR 682.00
-
EUR 798.00
|
|
|
Description: Recombinant Human B-lymphocyte antigen CD19(CD19),partial expressed in in vitro E.coli expression system |
Human B-lymphocyte antigen CD19 (CD19) |
1-CSB-CF004888HUc7 |
Cusabio |
-
EUR 965.00
-
EUR 665.00
-
EUR 715.00
|
|
|
Description: Recombinant Human B-lymphocyte antigen CD19(CD19),partial expressed in in vitro E.coli expression system |
human umbilical vein endothelial cells, Peptide Aptamer, unlabeled |
AP-316-U |
Alpha Diagnostics |
5 mg |
Ask for price |
Sheep Red Blood Cells |
20R-RS001 |
Fitzgerald |
10 ml |
EUR 521 |
Description: Human IgG Senstitized Gluteraldehyde Stabilized Sheep Red Bllood Cells |
Bovine Red Blood Cells |
88R-B002 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Bovine Red Blood Cells |
Chicken Red Blood Cells |
88R-C002 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Chicken Red Blood Cells |
Dog Red Blood Cells |
88R-D004 |
Fitzgerald |
10 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Dog Red Blood Cells |
Goat Red Blood Cells |
88R-G003 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Goat Red Blood Cells |
Hamster Red Blood Cells |
88R-H001 |
Fitzgerald |
5 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Hamster Red Blood Cells |
Horse Red Blood Cells |
88R-H002 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Horse Red Blood Cells |
Hamster Red Blood Cells |
88R-H003 |
Fitzgerald |
10 ml |
EUR 489 |
Description: Washed and freshly prepared 10% suspension of Hamster Red Blood Cells |
Mouse Red Blood Cells |
88R-M001 |
Fitzgerald |
10 ml |
EUR 295 |
Description: Washed and freshly prepared 10% suspension of Mouse Red Blood Cells |
Monkey Red Blood Cells |
88R-M003 |
Fitzgerald |
5 x 2 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Monkey Red Blood Cells |
Mouse Red Blood Cells |
88R-M004 |
Fitzgerald |
5 x 2 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Mouse Red Blood Cells |
Pig Red Blood Cells |
88R-P002 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Pig Red Blood Cells |
Rabbit Red Blood Cells |
88R-R001 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Rabbit Red Blood Cells |
Rat Red Blood Cells |
88R-R002 |
Fitzgerald |
5 x 2.0ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Rat Red Blood Cells |
Rat Red Blood Cells |
88R-R005 |
Fitzgerald |
25 ml |
EUR 327 |
Description: Washed and freshly prepared 10% suspension of Rat Red Blood Cells |
Sheep Red Blood Cells |
88R-S001 |
Fitzgerald |
100 ml |
EUR 512 |
Description: Washed and freshly prepared 10% suspension of Sheep Red Blood Cells |
Sheep Red Blood Cells |
88R-S003 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Sheep Red Blood Cells |
Sheep Red Blood Cells |
88R-S004 |
Fitzgerald |
10 ml |
EUR 521 |
Description: Glutaraldehyde-stabilized freshly prepared albumin sensitised Sheep Red Blood Cells |
Sheep Red Blood Cells |
88R-S005 |
Fitzgerald |
10 ml |
EUR 446 |
Description: Glutaraldehyde-stabilized freshly prepared tanned Sheep Red Blood Cells |
Turkey Red Blood Cells |
88R-T001 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Turkey Red Blood Cells |
Mouse Monoclonal Anti-Human CD19 FITC/CD2 PE |
CD19-D1-50 |
Alpha Diagnostics |
50 tests |
EUR 651 |
Mouse Monoclonal Anti-Human CD19 FITC/CD3 PE |
CD19-D2-50 |
Alpha Diagnostics |
50 tests |
EUR 651 |
Mouse Monoclonal Anti-Human CD19 FITC/CD5 PE |
CD19-D3-50 |
Alpha Diagnostics |
50 tests |
EUR 651 |
Unprocessed Human Cord Blood from IRB-approved donors, Fresh |
FC-001-F10 |
101Bio |
- |
Ask for price |
Unprocessed Human Cord Blood from IRB-approved donors, Fresh |
FC-001-F100 |
101Bio |
- |
Ask for price |
Unprocessed Human Cord Blood from IRB-approved donors, Fresh |
FC-001-F50 |
101Bio |
- |
Ask for price |
Unprocessed Human Cord Blood from IRB-approved donors, Fresh |
FC-001-F80 |
101Bio |
- |
Ask for price |
Mouse Umbilical Cord FibrOut: Fibroblast Inhibitory System (for 500 ml medium) |
7-15184 |
CHI Scientific |
1 Kit |
Ask for price |
Rat Umbilical Cord FibrOut: Fibroblast Inhibitory System (for 500 ml medium) |
7-15264 |
CHI Scientific |
1 Kit |
Ask for price |
human umbilical vein endothelial cells, Peptide Aptamer, FITC labelled |
AP-316-F |
Alpha Diagnostics |
1 mg |
Ask for price |
CORNING®HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS, HUVEC-2 CELLS (>5X105 CELLS), 1 CRYOVIAL/PACK |
354151 |
CORNING |
1/pk |
EUR 388 |
Description: Permeable Support - DL; Coated Transwells - DL |
B-Lymphocyte Antigen CD19 (CD19) Antibody |
abx011636-100ul |
Abbexa |
100 ul |
EUR 411 |
|
B-Lymphocyte Antigen CD19 (CD19) Antibody |
abx159376-100ul |
Abbexa |
100 ul |
EUR 356 |
|
B-Lymphocyte Antigen CD19 (CD19) Antibody |
abx159377-100ul |
Abbexa |
100 ul |
EUR 356 |
|
B-Lymphocyte Antigen CD19 (CD19) Antibody |
abx159378-100ul |
Abbexa |
100 ul |
EUR 356 |
|
B-Lymphocyte Antigen CD19 (CD19) Antibody |
abx159379-100ul |
Abbexa |
100 ul |
EUR 356 |
|
B-Lymphocyte Antigen CD19 (CD19) Antibody |
abx159380-100ul |
Abbexa |
100 ul |
EUR 439 |
|
B-Lymphocyte Antigen CD19 (CD19) Antibody |
abx159381-100ul |
Abbexa |
100 ul |
EUR 356 |
|
Rabbit Red Blood Cells (100%) |
20-abx800015 |
Abbexa |
|
|
|
Sheep Red Blood Cells (100%) |
20-abx800032 |
Abbexa |
|
|
|
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