Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System

November 12, 2020
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eaaci2015

Effects of 660-nm and 810-nm Low-Power Diode Laser on Proliferation and Invasion of Oral Cancer Cells in Cell Culture Media

 

In the present study, the effects of 660- and 810-nm diode laser on the proliferation and invasion of cancer cells were investigated. Sixteen plates of oral cancer cells originated from tongue SCC were irradiated with diode laser at 660-nm (40 and 80 mW) and 810-nm (100 and 200 mW) with the energy density of 4 J/cm2. One plate received no irradiation (the control). Irradiation was performed in four times (0,24,72 and 168 hours). Cell proliferation was measured by MTT assay.
The Ki67 and vascular endothelial growth factor (VEGF) markers were examined by real-time polymerase chain reaction (RT-PCR). Cyclin D1, E-cadherin, β-catenin, matrix metalloproteinase-9 (MMP-9; flow cytometry), were also evaluated. Proliferation was lower in the irradiated groups. This result was significant for all groups at the 24-hour. The percentages of Cyclin D1and MMP-9 were higher in 810nm groups, β-catenin and E-cadherin were higher in 660 nm groups, VEGF marker was significantly lower in 810 nm/200 mW group and Ki67 marker was no difference between the groups. According to the results of this study, laser irradiation at 0- and 24-h resulted in a significant inhibitory effect on cell proliferation especially in 660-nm / 80-mW and 810-nm / 200-mW. Further studies are needed in this respect.

A Biologist’s Guide to Traction Force Microscopy Using Polydimethylsiloxane Substrate for Two-Dimensional Cell Cultures

 

Cellular traction forces influence epithelial behavior, including wound healing and cell extrusion. Here, we describe a simple in vitro traction force microscopy (TFM) protocol using ECM protein-coated polydimethylsiloxane substrate and widefield fluorescence microscopy. We include detailed steps for analysis so readers can obtain traction forces to study the mechanobiology of epithelial cells. We also provide guidelines on when to adopt another common class of TFM protocols based on polyacrylamide hydrogels. For complete details on the use and execution of this protocol, please refer to Saw et al. (parafina) and Teo et al. (Gentaur).
Paraffin Wax Dispenser
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
MagSi-WAX
MagSi-WAX
MagSi-WAX
Wax Paper For Se235 Pkg100
neo WAX 30mg Plate SPE Columns
neo WAX 100mg/3mL SPE Columns
neo WAX 200mg/6mL SPE Columns

Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System

 

Melanocytes, derived from neural crest cells, are involved in melanin production. This protocol describes a method to generate induced melanocytes (iMelanocytes) from human induced pluripotent stem cells (iPSCs) using a suspension culture system, which considerably improves the differentiation efficiency. The most critical parts of this protocol are the selection of a reliable iPSC line with strong potential to differentiate into melanocytes and their stemness maintenance. For complete information on the use and generation of this protocol, please refer to our Cell Reports article, Liu el al. (2019).

Breast Cancer Cells Induce a Pro-inflammatory Response to Mitigate Immune Mediation in a 3D Culture Model

Background: Growth factors and cytokines mediate complex interactions between cells within the breast tumour microenvironment. In advanced cancer, an excess of regulatory T (TREG) lymphocytes and lack of natural killer (NK) cells in tumour-infiltrating lymphocyte populations may reflect a shift to pro-tumorigenic adaptive immune mechanisms. To facilitate targeted assessment of the interactions between tumour and immune cells ex vivo, three-dimensional (3D) culture systems are able to better recapitulate the in vivo microenvironment, recreating the anatomy of tumours.
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eaaci2015

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ALT. THIOGLYCOLLATE MED.
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ALT. THIOGLYCOLLATE MED.
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Bouin's Fluid
BNF125 125 ml
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Bouin's Fluid
BNF3800 1 Gal.
EUR 213.6

Bouin's Fluid
BNF500 500 ml
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Bouin's Fluid
BNF999 1000 ml
EUR 117.6

Pereneys Fluid
PFLH 500ML
EUR 145.92

Michel's Transport Fluid
MTF-10000 10 L
EUR 914.4

Michel's Transport Fluid
MTF-20000 20 L
EUR 1608

Michel's Transport Fluid
MTF500 500 ml
EUR 117.6

Michel's Transport Fluid
MTF999 1000 ml
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SOF Synthetic Oviduct Fluid.
IVL05-100ML 100 ml
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Description: Synthetic Oviduct Fluid.

SOF Synthetic Oviduct Fluid.
IVL05-6X100ML 6 x 100 ml
EUR 181.2
Description: Synthetic Oviduct Fluid.

G bath fluid 5L
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G bath fluid 10L
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M bath fluid 10L
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HS bath fluid 10L
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H10 bath fluid 10L
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MagSi-DNA Body Fluid
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MagSi-DNA Body Fluid
MDKT00140960 10 x 96 preps
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HE4, Human ascites fluid
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HE4, Human ascites fluid
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20 x Synovial Fluid Solution
GR103059 100 mL
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Immobiline Drystrip cover fluid 1000mL
17133501 EACH
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CA-125, Human Ascites Fluid
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Mounting Medium
4300 3 ml
EUR 216.6
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein.

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COLIFORM MEDIUM
C03-127-2kg 2kg
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COLIFORM MEDIUM
C03-127-500g 500 g
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Heller's Medium
CP014-010 10X1L
EUR 118.8

Heller's Medium
CP014-500 50L
EUR 151.2

Hoagland's Medium
CP015-010 10X1L
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Hoagland's Medium
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A Medium
DJ1018 100g
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EC MEDIUM
E05-100-10kg 10 kg
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EC MEDIUM
E05-100-2Kg 2 Kg
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EC MEDIUM
E05-100-500g 500 g
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NeuroProgenitor Medium
NM42400 125 ml
EUR 364.8

HLP MEDIUM
H08-107-10kg 10 kg
EUR 2733.6

HLP MEDIUM
H08-107-2Kg 2 Kg
EUR 640.8

HLP MEDIUM
H08-107-500g 500 g
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M9 Medium
SD7024 250g
EUR 86.1

M9CA Medium
SD7025 250g
EUR 86.1

YM Medium
SD7031 250g
EUR 84.54

TYGPN Medium
SD7032 250g
EUR 84.01

M63 Medium
SD7033 250g
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SIM MEDIUM
S19-110-10kg 10 kg
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S19-110-2kg 2kg
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SIM MEDIUM
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SOB MEDIUM
S19-124-10kg 10 kg
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SOB MEDIUM
S19-124-2kg 2kg
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SOB MEDIUM
S19-124-500g 500 g
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TCBS Medium
MED1188 PK10
EUR 7.52

DNase Medium
MED1196 PK10
EUR 7.98

OF Medium
MED1664 500G
EUR 68.4

BD FACS Flow Sheath Fluid 20L
342003 EACH
EUR 78.66

Cancer Antigen 125 Antigen (Ascites Fluid)
VAng-Cr3870-10kU 10 kU
EUR 1132.8
Description: Cancer Antigen 125 Antigen, Host/Source: Ascites Fluid. The purity is ≥ 95% by SDS-PAGE.

Cryoscope Heat Transfer Fluid Dairy Testing
HTF250 250ML
EUR 36.48

Purified Exosomes: Human Biofluids Ascites Fluid
EXOP-550A-1 ≥ 25 µg
EUR 611

Insect Cell Medium: TNM-FH Insect Culture Medium
ABP-MED-10001 1 liter Ask for price

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A01-116-500g 500 g
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Recovery Medium - 6x12mL
1711 4/PK
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Aqueous Mounting Medium
AR1018 10mL X5 (Enough for 800-1200 assays)
EUR 96

Antifade Mounting Medium
AR1109 10mL (for 1000 assays)
EUR 78

MCDB 131 Medium
CM034-050 500ml
EUR 102

MCDB 131 Medium
CM034-300 6x500ml
EUR 198

MCDB 131 Medium
CM034-310 10x500ml
EUR 262.8

MCDB 131 Medium
CM034-320 20x500ml
EUR 408

MCDB 131 Medium
CM034-350 50x500ml
EUR 702

Williams' Medium E
CM063-050 500ml
EUR 109.2

Williams' Medium E
CM063-300 6x500ml
EUR 319.2

Williams' Medium E
CM063-310 10x500ml
EUR 375.6

Williams' Medium E
CM063-320 20x500ml
EUR 724.8

Williams' Medium E
CM063-350 50x500ml
EUR 1170

AddexBio Cryopreservation Medium
C0002-20mL RT vial
EUR 124.8

CARY & BLAIR MEDIUM
C03-131-10kg 10 kg
EUR 2622

CARY & BLAIR MEDIUM
C03-131-2Kg 2 Kg
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CARY & BLAIR MEDIUM
C03-131-500g 500 g
EUR 211.2

Cereal Crops Medium
CP003-005 5L
EUR 135.6

Cereal Crops Medium
CP003-010 10X1L
EUR 202.8

Kao Michayluk Medium
CP017-010 10X1L
EUR 118.8

Kao Michayluk Medium
CP017-500 50L
EUR 151.2

Orchid Maintenance Medium
CP037-010 10X1L
EUR 130.8

Orchid Maintenance Medium
CP037-500 50L
EUR 165.6

Woody Plants Medium
CP040-010 10X1L
EUR 121.2

Woody Plants Medium
CP040-500 50L
EUR 151.2

H Medium Broth
DJ1019 100g
EUR 73.06

EC MEDIUM, MODIFIED
E05-108-10kg 10 kg
EUR 1029.6

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E05-108-2Kg 2 Kg
EUR 271.2

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E05-108-500g 500 g
EUR 117.6

MOTILITY TEST MEDIUM
M13-121-10kg 10 kg
EUR 1833.6
Materials and methods: We used 3D breast tumour models to determine morphological alterations, and the levels of secreted transforming growth factor-β (TGFβ) and induced cytokines. 3D luminal phenotype models and basal phenotype models were generated by culturing NK cells and CD4+CD25+ TREG cells with MCF-7 cells and MDA-MB-231 cells respectively, in growth factor-reduced Matrigel. TGFβ was qualitatively assessed by immunolocalisation and cytokine data from culture supernatant was acquired with a multiplex cytokine assay. Traditional statistical analysis and principal component analysis were employed to unravel the cytokine response.
Results and conclusion: We identified that an interleukin-6 (IL6)-chemokine axis associated with TGFβ is primarily responsible for differences detected between breast cancer models, with luminal and basal phenotype tumours responding differentially to immune mediation. Identified cytokines are implicated in facilitating tumour cell subversion of immune cell function to promote an invasive phenotype. Moreover, the disruption of the extracellular matrix and failure to form well-differentiated tumour masses/networks is indicative of enhanced malignancy. Tumour cells are implicated in promoting a pro-inflammatory microenvironment to attenuate NK cell function and in inducing a pro-tumorigenic profile that is facilitated by TREG lymphocytes.

Growing human trophoblasts in vitro: a review of the media commonly used in trophoblast cell culture

Trophoblasts are unique epithelial cells found only in the placenta. It has been possible to isolate and maintain human trophoblasts in in vitro culture for many decades. During this period there have been a vast array of media and supplements reported for trophoblast culture and often the reasons for using the media and specific supplements employed in any given laboratory have been lost in the ‘mists of time’. After a gradual development over many years this field has recently changed, with the publication of several reports of the isolation, growth and differentiation of human trophoblast stem or stem-like cells.
This advance was made largely because of a greater understanding of the molecular pathways that control human trophoblasts and availability of media supplements that can be used to manipulate those pathways. We have searched the literature and here summarise many of the different media and supplements and describe how and why they were developed and are used to culture human trophoblasts.

Establishment of Conditions for Long-Term Maintenance of Primary Embryonic Cell Cultures from Olive Flounder Paralichthys olivaceus

Primary cell culture is a sufficient method frequently used to study the cellular properties and mechanisms of isolated cells in a controlled environment. In this study, an embryonic cell line (FGBC8) derived from the blastula stages of embryos of olive flounder Paralichthys olivaceus was developed. Furthermore, conditions for optimal long-term maintenance of this primary embryonic cell culture were investigated. Morphologically, FGBC8 cells were composed primarily of epithelial-like cells. FGBC8 cells were subcultured for >160 passages over ~830 days.
The doubling time of FGBC8 cells was 73.8 h, and the modal diploid chromosome number was 48. FGBC8 cells transfected with green fluorescence protein (GFP)-expression plasmid exhibited a strong signal 48 h after transfection. Consequently, we demonstrated that fish serum is a crucial supplement for the long-term survival and maintenance of comparable morphology in these primary embryonic cells. Our results can be used as a guide for primary embryonic cell cultures for other fish species and may be useful for cell biotechnological applications.

NBS Medium Test Tube Rack 8-11 Mm Size
SHA4410 EACH
EUR 363.66

NBS Medium Test Tube Rack 12-15 Mm Size
SHA4412 EACH
EUR 363.66

NBS Medium Test Tube Rack 15-18 Mm Size
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EUR 363.66

NBS Medium Test Tube Rack 18-21 Mm Size
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NBS Medium Test Tube Rack 22-26 Mm Size
SHA4418 EACH
EUR 363.66

Test Tubes 50 x 6.5mm rimless 0.65mm medium wall
TES1030 PK250
EUR 67.72

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TES1032 PK250
EUR 45.94

Test Tubes 75 x 10mm rimless 1.0mm medium wall
TES1034 PK100
EUR 18.35

Test Tubes 75 x 12mm rimless 1.0mm medium wall
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EUR 24.05

Test Tubes 100 x 12mm rimless 1.0mm medium wall
TES1038 PK100
EUR 26.68

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TES1040 PK100
EUR 35

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EUR 38.65

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TES1046 PK100
EUR 38.99

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TES1048 PK100
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EUR 34.88

Test Tubes 75 x 10mm rimless 1.0mm medium wall
TES1220 PK100
EUR 28.04

Test Tubes 75 x 12mm rimless 1.0mm medium wall
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Test Tubes 100 x 12mm rimless 1.0mm medium wall
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TES1232 PK100
EUR 71.59

Test Tubes 150 x 24mm rimless 1.2mm medium wall
TES1234 PK50
EUR 48.68

Cell Freezing Medium
79796-1 50 ml
EUR 140
Description: Cryopreservation is a cornerstone of cell biology, and is essential to maintaining healthy and reliable cell lines. BPS Bioscience Cell Freezing Medium is optimized for maximum viability and rapid recovery of all BPS Bioscience Cell Lines. This product contains DMSO. It is provided sterile-filtered and mycoplasma-free for the protection of the samples.

Cell Freezing Medium
79796-2 100 ml
EUR 275
Description: Cryopreservation is a cornerstone of cell biology, and is essential to maintaining healthy and reliable cell lines. BPS Bioscience Cell Freezing Medium is optimized for maximum viability and rapid recovery of all BPS Bioscience Cell Lines. This product contains DMSO. It is provided sterile-filtered and mycoplasma-free for the protection of the samples.

Recombinant human Isoform 3 of Neuronal cell adhesion molecule
P2740 100ug Ask for price
Description: Recombinant protein for human Isoform 3 of Neuronal cell adhesion molecule

Test Tubes 75 x 10mm with rim 1.0mm medium wall
TES1200 PK100
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EUR 31.24

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EUR 34.88

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Pyrex Test Tubes 75 x 10mm rimless 1mm medium wall
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EUR 64.41

Pyrex Test Tubes 100 x 12mm rimless 1mm medium wall
TES2034 PK100
EUR 70.22

Pyrex Test Tubes 100 x 16mm rimless 1.2mm medium wall
TES2036 PK100
EUR 91.47

Pyrex Test Tubes 125 x 16mm rimless 1.2mm medium wall
TES2038 PK100
EUR 91.47

Pyrex Test Tubes 150 x 16mm rimless 1.2mm medium wall
TES2040 PK100
EUR 91.47

Pyrex Test Tubes 160 x 16mm rimless 1.2mm medium wall
TES2042 PK100
EUR 109.51

Pyrex Test Tubes 180 x 18mm rimless 1.2mm medium wall
TES2044 PK100
EUR 114.65

Pyrex Test Tubes 150 x 18mm rimless 1.2mm medium wall
TES2046 PK100
EUR 110.79

Pyrex Test Tubes 150 x 24mm rimless 1.2mm medium wall
TES2048 PK100
EUR 163.6

Pyrex Test Tubes 200 x 24mm rimless 1.2mm medium wall
TES2050 PK50
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Recombinant human Neurofilament medium polypeptide
P1623 100ug Ask for price
Description: Recombinant protein for human Neurofilament medium polypeptide

Test Tube Cell Holder
SPE9206 EACH
EUR 599.64

1-Step Polymorphs, Human Cell Separation
71-AN221725 1
EUR 238.8

Pyrex Test Tubes 75 x 10mm with rim 1mm medium wall
TES2000 PK100
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TES2016 PK100
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TES2018 PK100
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Insect Cell Medium: TNM-FH Insect Culture Medium
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Insect Cell Medium: Serum-Free Insect Culture Medium
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Insect Cell Medium: Grace?s Insect Medium (Unsupplemented)
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Stellate Cell Medium/kit
C0009-01 RT 500 mL Bottle
EUR 272.4

Cervical Epithalial cell medium
C0011-01 RT 500 mL Bottle
EUR 286.8

Human Epithelial Cell Medium
ECM001 500 mL
EUR 387.6

Accu-Tell COVID-19 IgG/IgM Rapid Test
GEN-B352-20tests 20 tests
EUR 283.2
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Accu-Tell COVID-19 IgG/IgM Rapid Test
GEN-B352-40tests 40 tests
EUR 385.2
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Rat NNE(Non-Neuronal Enolase) ELISA Kit
ER1191 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.188 ng/ml

Human NNE(Non-Neuronal Enolase) ELISA Kit
EH0371 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

Human NPTXR(Neuronal pentraxin receptor) ELISA Kit
EH14986 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

Human P311(Neuronal protein 3.1) ELISA Kit
EH4926 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Gryphon Packaging Cell Selection Medium
ABP-TC-PHSM050 50 ml Ask for price
Description: PRODUCT LINE DISCONTINUED. Please contact us.

PK15 Cell Serum-Free Medium
VCum-Lsx0007 1 L
EUR 909.6
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.

CHO Cell Serum-Free Medium
VCum-Lsx0025 1L
EUR 762
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).

HEK Cell Serum-Free Medium
VCum-Lsx0027 1L
EUR 762
Description: A serum-free medium that is ideal for suspension culture of HEK cell lines.

MDBK Cell Serum-Free Medium
VCum-Lsx0029 1L
EUR 818.4
Description: Used in serum-free suspension MDBK cell line culture for bovine virus diarrhea or bovine rhinotracheitis vaccine production.

Vero Cell Serum-Free Medium
VCum-Lsx0031 1 L
EUR 909.6
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.

Gibco CHO Cell Culture Medium
220253 EACH
EUR 50.05

Test product
testttt 0 Ask for price

Human NEGR1(Neuronal growth regulator 1) ELISA Kit
EH14985 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Human SEPT3(Neuronal-specific septin-3) ELISA Kit
EH1743 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

Recombinant human Neuronal acetylcholine receptor subunit alpha-3
P1221 100ug Ask for price
Description: Recombinant protein for human Neuronal acetylcholine receptor subunit alpha-3

Recombinant human Neuronal acetylcholine receptor subunit beta-2
P2924 100ug Ask for price
Description: Recombinant protein for human Neuronal acetylcholine receptor subunit beta-2

Human proPSA(precursor of Prostate Specific Antigen) ELISA Kit
EH3659 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.281 ng/ml

Monkey AMBP(Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit
EMK0004 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Ape;Sensitivity: 0.188 ng/ml

Human ANNA1(Anti-Neuronal Nuclear Antibody 1) ELISA Kit
EH2638 96T
EUR 628.92
Description: Method of detection: Sandwich ELISA, Double Antigen;Reacts with: Homo sapiens;Sensitivity: 0.938 ng/ml

2019-nCoV IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma)
GEN-402-25tests 25 tests
EUR 292.8
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Mounting Medium
4300 3 ml
EUR 216.6
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein.

DC MEDIUM
D04-117-10kg 10 kg
EUR 1894.8

DC MEDIUM
D04-117-2kg 2kg
EUR 458.4

DC MEDIUM
D04-117-500g 500 g
EUR 168

Advanced Medium
C0003-04 RT 500 mL Bottle
EUR 123.6

Medium 199
C0012-01 RT 500 mL Bottle
EUR 126

COLIFORM MEDIUM
C03-127-10kg 10 kg
EUR 1588.8

COLIFORM MEDIUM
C03-127-2kg 2kg
EUR 392.4

 

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