Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System

November 12, 2020
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eaaci2015

Effects of 660-nm and 810-nm Low-Power Diode Laser on Proliferation and Invasion of Oral Cancer Cells in Cell Culture Media

 

In the present study, the effects of 660- and 810-nm diode laser on the proliferation and invasion of cancer cells were investigated. Sixteen plates of oral cancer cells originated from tongue SCC were irradiated with diode laser at 660-nm (40 and 80 mW) and 810-nm (100 and 200 mW) with the energy density of 4 J/cm2. One plate received no irradiation (the control). Irradiation was performed in four times (0,24,72 and 168 hours). Cell proliferation was measured by MTT assay.
The Ki67 and vascular endothelial growth factor (VEGF) markers were examined by real-time polymerase chain reaction (RT-PCR). Cyclin D1, E-cadherin, β-catenin, matrix metalloproteinase-9 (MMP-9; flow cytometry), were also evaluated. Proliferation was lower in the irradiated groups. This result was significant for all groups at the 24-hour. The percentages of Cyclin D1and MMP-9 were higher in 810nm groups, β-catenin and E-cadherin were higher in 660 nm groups, VEGF marker was significantly lower in 810 nm/200 mW group and Ki67 marker was no difference between the groups. According to the results of this study, laser irradiation at 0- and 24-h resulted in a significant inhibitory effect on cell proliferation especially in 660-nm / 80-mW and 810-nm / 200-mW. Further studies are needed in this respect.

A Biologist’s Guide to Traction Force Microscopy Using Polydimethylsiloxane Substrate for Two-Dimensional Cell Cultures

 

Cellular traction forces influence epithelial behavior, including wound healing and cell extrusion. Here, we describe a simple in vitro traction force microscopy (TFM) protocol using ECM protein-coated polydimethylsiloxane substrate and widefield fluorescence microscopy. We include detailed steps for analysis so readers can obtain traction forces to study the mechanobiology of epithelial cells. We also provide guidelines on when to adopt another common class of TFM protocols based on polyacrylamide hydrogels. For complete details on the use and execution of this protocol, please refer to Saw et al. (parafina) and Teo et al. (Gentaur).
Paraffin Wax Dispenser
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
MagSi-WAX
MagSi-WAX
MagSi-WAX
Wax Paper For Se235 Pkg100
neo WAX 30mg Plate SPE Columns
neo WAX 100mg/3mL SPE Columns
neo WAX 200mg/6mL SPE Columns

Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System

 

Melanocytes, derived from neural crest cells, are involved in melanin production. This protocol describes a method to generate induced melanocytes (iMelanocytes) from human induced pluripotent stem cells (iPSCs) using a suspension culture system, which considerably improves the differentiation efficiency. The most critical parts of this protocol are the selection of a reliable iPSC line with strong potential to differentiate into melanocytes and their stemness maintenance. For complete information on the use and generation of this protocol, please refer to our Cell Reports article, Liu el al. (2019).

Breast Cancer Cells Induce a Pro-inflammatory Response to Mitigate Immune Mediation in a 3D Culture Model

Background: Growth factors and cytokines mediate complex interactions between cells within the breast tumour microenvironment. In advanced cancer, an excess of regulatory T (TREG) lymphocytes and lack of natural killer (NK) cells in tumour-infiltrating lymphocyte populations may reflect a shift to pro-tumorigenic adaptive immune mechanisms. To facilitate targeted assessment of the interactions between tumour and immune cells ex vivo, three-dimensional (3D) culture systems are able to better recapitulate the in vivo microenvironment, recreating the anatomy of tumours.
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eaaci2015

SODIUM THIOGLYCOLLATE
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MED1126 PK50
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ALT. THIOGLYCOLLATE MED.
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ALT. THIOGLYCOLLATE MED.
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Bouin's Fluid
BNF125 125 ml
EUR 75.6

Bouin's Fluid
BNF3800 1 Gal.
EUR 213.6

Bouin's Fluid
BNF500 500 ml
EUR 96

Bouin's Fluid
BNF999 1000 ml
EUR 117.6

Pereneys Fluid
PFLH 500ML
EUR 145.92

Michel's Transport Fluid
MTF-10000 10 L
EUR 914.4

Michel's Transport Fluid
MTF-20000 20 L
EUR 1608

Michel's Transport Fluid
MTF500 500 ml
EUR 117.6

Michel's Transport Fluid
MTF999 1000 ml
EUR 152.4

HE4, Human ascites fluid
P1438-100
EUR 744

HE4, Human ascites fluid
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MagSi-DNA Body Fluid
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MagSi-DNA Body Fluid
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SOF Synthetic Oviduct Fluid.
IVL05-100ML 100 ml
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Description: Synthetic Oviduct Fluid.

SOF Synthetic Oviduct Fluid.
IVL05-6X100ML 6 x 100 ml
EUR 181.2
Description: Synthetic Oviduct Fluid.

Fluid dispenser 120cc pink
INS2200 EACH
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G bath fluid 5L
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CA-125, Human Ascites Fluid
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20 x Synovial Fluid Solution
GR103059 100 mL
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Immobiline Drystrip cover fluid 1000mL
17133501 EACH
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A Medium
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COLIFORM MEDIUM
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EUR 150

Heller's Medium
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EUR 118.8

Heller's Medium
CP014-500 50L
EUR 151.2

Hoagland's Medium
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EUR 118.8

Hoagland's Medium
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EUR 151.2

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EC MEDIUM
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EC MEDIUM
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Mounting Medium
4300 3 ml
EUR 216.6
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein.

NeuroProgenitor Medium
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EUR 364.8

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EUR 84.54

TYGPN Medium
SD7032 250g
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M63 Medium
SD7033 250g
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MED1188 PK10
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MED1664 500G
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Cancer Antigen 125 Antigen (Ascites Fluid)
VAng-Cr3870-10kU 10 kU
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Description: Cancer Antigen 125 Antigen, Host/Source: Ascites Fluid. The purity is ≥ 95% by SDS-PAGE.

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HTF250 250ML
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BD FACS Flow Sheath Fluid 20L
342003 EACH
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CM034-050 500ml
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CM034-350 50x500ml
EUR 702

Williams' Medium E
CM063-050 500ml
EUR 109.2

Williams' Medium E
CM063-300 6x500ml
EUR 319.2

Williams' Medium E
CM063-310 10x500ml
EUR 375.6

Williams' Medium E
CM063-320 20x500ml
EUR 724.8

Williams' Medium E
CM063-350 50x500ml
EUR 1170

H Medium Broth
DJ1019 100g
EUR 73.06

AddexBio Cryopreservation Medium
C0002-20mL RT vial
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C03-131-10kg 10 kg
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C03-131-2Kg 2 Kg
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C03-131-500g 500 g
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Cereal Crops Medium
CP003-005 5L
EUR 135.6

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CP003-010 10X1L
EUR 202.8

Kao Michayluk Medium
CP017-010 10X1L
EUR 118.8

Kao Michayluk Medium
CP017-500 50L
EUR 151.2

Orchid Maintenance Medium
CP037-010 10X1L
EUR 130.8

Orchid Maintenance Medium
CP037-500 50L
EUR 165.6

Woody Plants Medium
CP040-010 10X1L
EUR 121.2

Woody Plants Medium
CP040-500 50L
EUR 151.2

EC MEDIUM, MODIFIED
E05-108-10kg 10 kg
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EC MEDIUM, MODIFIED
E05-108-2Kg 2 Kg
EUR 271.2

EC MEDIUM, MODIFIED
E05-108-500g 500 g
EUR 117.6

FluoreGuard Mounting Medium
FMM030 30 ml
EUR 115.2

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FMM060 60 ml
EUR 139.2

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FMM500 500 ml
EUR 392.4

FluoreGuard Mounting Medium
FMM999 1000 ml
EUR 636

Recovery Medium - 6x12mL
1711 4/PK
EUR 153.6
Materials and methods: We used 3D breast tumour models to determine morphological alterations, and the levels of secreted transforming growth factor-β (TGFβ) and induced cytokines. 3D luminal phenotype models and basal phenotype models were generated by culturing NK cells and CD4+CD25+ TREG cells with MCF-7 cells and MDA-MB-231 cells respectively, in growth factor-reduced Matrigel. TGFβ was qualitatively assessed by immunolocalisation and cytokine data from culture supernatant was acquired with a multiplex cytokine assay. Traditional statistical analysis and principal component analysis were employed to unravel the cytokine response.
Results and conclusion: We identified that an interleukin-6 (IL6)-chemokine axis associated with TGFβ is primarily responsible for differences detected between breast cancer models, with luminal and basal phenotype tumours responding differentially to immune mediation. Identified cytokines are implicated in facilitating tumour cell subversion of immune cell function to promote an invasive phenotype. Moreover, the disruption of the extracellular matrix and failure to form well-differentiated tumour masses/networks is indicative of enhanced malignancy. Tumour cells are implicated in promoting a pro-inflammatory microenvironment to attenuate NK cell function and in inducing a pro-tumorigenic profile that is facilitated by TREG lymphocytes.

Growing human trophoblasts in vitro: a review of the media commonly used in trophoblast cell culture

Trophoblasts are unique epithelial cells found only in the placenta. It has been possible to isolate and maintain human trophoblasts in in vitro culture for many decades. During this period there have been a vast array of media and supplements reported for trophoblast culture and often the reasons for using the media and specific supplements employed in any given laboratory have been lost in the ‘mists of time’. After a gradual development over many years this field has recently changed, with the publication of several reports of the isolation, growth and differentiation of human trophoblast stem or stem-like cells.
This advance was made largely because of a greater understanding of the molecular pathways that control human trophoblasts and availability of media supplements that can be used to manipulate those pathways. We have searched the literature and here summarise many of the different media and supplements and describe how and why they were developed and are used to culture human trophoblasts.

Establishment of Conditions for Long-Term Maintenance of Primary Embryonic Cell Cultures from Olive Flounder Paralichthys olivaceus

Primary cell culture is a sufficient method frequently used to study the cellular properties and mechanisms of isolated cells in a controlled environment. In this study, an embryonic cell line (FGBC8) derived from the blastula stages of embryos of olive flounder Paralichthys olivaceus was developed. Furthermore, conditions for optimal long-term maintenance of this primary embryonic cell culture were investigated. Morphologically, FGBC8 cells were composed primarily of epithelial-like cells. FGBC8 cells were subcultured for >160 passages over ~830 days.
The doubling time of FGBC8 cells was 73.8 h, and the modal diploid chromosome number was 48. FGBC8 cells transfected with green fluorescence protein (GFP)-expression plasmid exhibited a strong signal 48 h after transfection. Consequently, we demonstrated that fish serum is a crucial supplement for the long-term survival and maintenance of comparable morphology in these primary embryonic cells. Our results can be used as a guide for primary embryonic cell cultures for other fish species and may be useful for cell biotechnological applications.

NBS Medium Test Tube Rack 15-18 Mm Size
SHA4402 EACH
EUR 363.66

NBS Medium Test Tube Rack 8-11 Mm Size
SHA4410 EACH
EUR 363.66

NBS Medium Test Tube Rack 12-15 Mm Size
SHA4412 EACH
EUR 363.66

NBS Medium Test Tube Rack 15-18 Mm Size
SHA4414 EACH
EUR 363.66

NBS Medium Test Tube Rack 18-21 Mm Size
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NBS Medium Test Tube Rack 22-26 Mm Size
SHA4418 EACH
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Test Tubes 50 x 6.5mm rimless 0.65mm medium wall
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EUR 26.68

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TES1040 PK100
EUR 35

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EUR 34.77

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TES1046 PK100
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EUR 71.59

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TES1234 PK50
EUR 48.68

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TES1200 PK100
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EUR 64.41

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EUR 70.22

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EUR 91.47

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TES2046 PK100
EUR 110.79

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TES2048 PK100
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Pyrex Test Tubes 200 x 24mm rimless 1.2mm medium wall
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Recombinant human Isoform 3 of Neuronal cell adhesion molecule
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Recombinant human Neurofilament medium polypeptide
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Test Tube Cell Holder
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EUR 599.64

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Stellate Cell Medium/kit
C0009-01 RT 500 mL Bottle
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Cervical Epithalial cell medium
C0011-01 RT 500 mL Bottle
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Human Epithelial Cell Medium
ECM001 500 mL
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Accu-Tell COVID-19 IgG/IgM Rapid Test
GEN-B352-20tests 20 tests
EUR 283.2
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Accu-Tell COVID-19 IgG/IgM Rapid Test
GEN-B352-40tests 40 tests
EUR 385.2
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Rat NNE(Non-Neuronal Enolase) ELISA Kit
ER1191 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.188 ng/ml

Human NNE(Non-Neuronal Enolase) ELISA Kit
EH0371 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

Human NPTXR(Neuronal pentraxin receptor) ELISA Kit
EH14986 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

Human P311(Neuronal protein 3.1) ELISA Kit
EH4926 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Test product
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Human MEP Cell Culture Medium (human megakaryocyte–erythroid progenitor cell culture medium)
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Human CD34+ Cell Culture Medium
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PK15 Cell Serum-Free Medium
VCum-Lsx0007 1 L
EUR 909.6
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.

CHO Cell Serum-Free Medium
VCum-Lsx0025 1L
EUR 762
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).

HEK Cell Serum-Free Medium
VCum-Lsx0027 1L
EUR 762
Description: A serum-free medium that is ideal for suspension culture of HEK cell lines.

MDBK Cell Serum-Free Medium
VCum-Lsx0029 1L
EUR 818.4
Description: Used in serum-free suspension MDBK cell line culture for bovine virus diarrhea or bovine rhinotracheitis vaccine production.

Vero Cell Serum-Free Medium
VCum-Lsx0031 1 L
EUR 909.6
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.

Gibco CHO Cell Culture Medium
220253 EACH
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Human NEGR1(Neuronal growth regulator 1) ELISA Kit
EH14985 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Human SEPT3(Neuronal-specific septin-3) ELISA Kit
EH1743 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

Recombinant human Neuronal acetylcholine receptor subunit alpha-3
P1221 100ug Ask for price
Description: Recombinant protein for human Neuronal acetylcholine receptor subunit alpha-3

Recombinant human Neuronal acetylcholine receptor subunit beta-2
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Description: Recombinant protein for human Neuronal acetylcholine receptor subunit beta-2

Human proPSA(precursor of Prostate Specific Antigen) ELISA Kit
EH3659 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.281 ng/ml

Monkey AMBP(Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit
EMK0004 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Ape;Sensitivity: 0.188 ng/ml

2019-nCoV IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma)
GEN-402-25tests 25 tests
EUR 292.8
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Mounting Medium
4300 3 ml
EUR 216.6
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein.

DC MEDIUM
D04-117-10kg 10 kg
EUR 1894.8

DC MEDIUM
D04-117-2kg 2kg
EUR 458.4

DC MEDIUM
D04-117-500g 500 g
EUR 168

Advanced Medium
C0003-04 RT 500 mL Bottle
EUR 123.6

Medium 199
C0012-01 RT 500 mL Bottle
EUR 126

COLIFORM MEDIUM
C03-127-10kg 10 kg
EUR 1588.8

COLIFORM MEDIUM
C03-127-2kg 2kg
EUR 392.4

 

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