Effects of 660-nm and 810-nm Low-Power Diode Laser on Proliferation and Invasion of Oral Cancer Cells in Cell Culture Media
In the present study, the effects of 660- and 810-nm diode laser on the proliferation and invasion of cancer cells were investigated. Sixteen plates of oral cancer cells originated from tongue SCC were irradiated with diode laser at 660-nm (40 and 80 mW) and 810-nm (100 and 200 mW) with the energy density of 4 J/cm2. One plate received no irradiation (the control). Irradiation was performed in four times (0,24,72 and 168 hours). Cell proliferation was measured by MTT assay.
The Ki67 and vascular endothelial growth factor (VEGF) markers were examined by real-time polymerase chain reaction (RT-PCR). Cyclin D1, E-cadherin, β-catenin, matrix metalloproteinase-9 (MMP-9; flow cytometry), were also evaluated. Proliferation was lower in the irradiated groups. This result was significant for all groups at the 24-hour. The percentages of Cyclin D1and MMP-9 were higher in 810nm groups, β-catenin and E-cadherin were higher in 660 nm groups, VEGF marker was significantly lower in 810 nm/200 mW group and Ki67 marker was no difference between the groups. According to the results of this study, laser irradiation at 0- and 24-h resulted in a significant inhibitory effect on cell proliferation especially in 660-nm / 80-mW and 810-nm / 200-mW. Further studies are needed in this respect.
A Biologist’s Guide to Traction Force Microscopy Using Polydimethylsiloxane Substrate for Two-Dimensional Cell Cultures
Cellular traction forces influence epithelial behavior, including wound healing and cell extrusion. Here, we describe a simple in vitro traction force microscopy (TFM) protocol using ECM protein-coated polydimethylsiloxane substrate and widefield fluorescence microscopy. We include detailed steps for analysis so readers can obtain traction forces to study the mechanobiology of epithelial cells. We also provide guidelines on when to adopt another common class of TFM protocols based on polyacrylamide hydrogels. For complete details on the use and execution of this protocol, please refer to Saw et al. (parafina) and Teo et al. (Gentaur).
Paraffin Wax Dispenser |
Paraffin wax, granular (56 - 60) |
Paraffin wax, granular (56 - 60) |
Paraffin wax, granular (56 - 60) |
Paraffin wax, granular (56 - 60) |
XH-90D Paraffin Wax Trimmer |
Paraffin |
Paraffin |
Paraffin |
Paraffin |
Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System
Melanocytes, derived from neural crest cells, are involved in melanin production. This protocol describes a method to generate induced melanocytes (iMelanocytes) from human induced pluripotent stem cells (iPSCs) using a suspension culture system, which considerably improves the differentiation efficiency. The most critical parts of this protocol are the selection of a reliable iPSC line with strong potential to differentiate into melanocytes and their stemness maintenance. For complete information on the use and generation of this protocol, please refer to our Cell Reports article, Liu el al. (2019).
Breast Cancer Cells Induce a Pro-inflammatory Response to Mitigate Immune Mediation in a 3D Culture Model
Background: Growth factors and cytokines mediate complex interactions between cells within the breast tumour microenvironment. In advanced cancer, an excess of regulatory T (TREG) lymphocytes and lack of natural killer (NK) cells in tumour-infiltrating lymphocyte populations may reflect a shift to pro-tumorigenic adaptive immune mechanisms. To facilitate targeted assessment of the interactions between tumour and immune cells ex vivo, three-dimensional (3D) culture systems are able to better recapitulate the in vivo microenvironment, recreating the anatomy of tumours.
eaaci2015
Fluid Thioglycollate Medium |
LQ026XX-50X20ML |
EWC Diagnostics |
1 unit |
EUR 27.25 |
Description: Fluid Thioglycollate Medium |
Fluid Thioglycollate Medium |
ME009-100G |
EWC Diagnostics |
1 unit |
EUR 9.83 |
Description: Fluid Thioglycollate Medium |
Fluid Thioglycollate Medium |
ME009-2.5KG |
EWC Diagnostics |
1 unit |
EUR 80.4 |
Description: Fluid Thioglycollate Medium |
Fluid Thioglycollate Medium |
ME009-500G |
EWC Diagnostics |
1 unit |
EUR 16.96 |
Description: Fluid Thioglycollate Medium |
Fluid Thioglycollate Medium |
ME009-5KG |
EWC Diagnostics |
1 unit |
EUR 140.79 |
Description: Fluid Thioglycollate Medium |
Fluid Thioglycollate Medium |
MM009-100G |
EWC Diagnostics |
1 unit |
EUR 9.83 |
Description: Fluid Thioglycollate Medium |
Fluid Thioglycollate Medium |
MM009-2.5KG |
EWC Diagnostics |
1 unit |
EUR 80.4 |
Description: Fluid Thioglycollate Medium |
Fluid Thioglycollate Medium |
MM009-500G |
EWC Diagnostics |
1 unit |
EUR 16.96 |
Description: Fluid Thioglycollate Medium |
Fluid Thioglycollate Medium |
MM009-5KG |
EWC Diagnostics |
1 unit |
EUR 140.79 |
Description: Fluid Thioglycollate Medium |
Fluid Thioglycollate Medium |
MU009-100G |
EWC Diagnostics |
1 unit |
EUR 9.83 |
Description: Fluid Thioglycollate Medium |
Fluid Thioglycollate Medium |
MU009-2.5KG |
EWC Diagnostics |
1 unit |
EUR 80.4 |
Description: Fluid Thioglycollate Medium |
Fluid Thioglycollate Medium |
MU009-500G |
EWC Diagnostics |
1 unit |
EUR 16.96 |
Description: Fluid Thioglycollate Medium |
Fluid Thioglycollate Medium |
MU009-5KG |
EWC Diagnostics |
1 unit |
EUR 140.79 |
Description: Fluid Thioglycollate Medium |
Fluid Thioglycollate Medium |
MU009G-2.5KG |
EWC Diagnostics |
1 unit |
EUR 95.32 |
Description: Fluid Thioglycollate Medium |
Fluid Thioglycollate Medium |
MU009G-500G |
EWC Diagnostics |
1 unit |
EUR 19.23 |
Description: Fluid Thioglycollate Medium |
Fluid Thioglycollate Medium |
MU009G-5KG |
EWC Diagnostics |
1 unit |
EUR 184.29 |
Description: Fluid Thioglycollate Medium |
Fluid Thioglycollate Medium |
M009B-100G |
EWC Diagnostics |
1 unit |
EUR 9.83 |
Description: Fluid Thioglycollate Medium |
Fluid Thioglycollate Medium |
M009B-2.5KG |
EWC Diagnostics |
1 unit |
EUR 80.4 |
Description: Fluid Thioglycollate Medium |
Fluid Thioglycollate Medium |
M009B-500G |
EWC Diagnostics |
1 unit |
EUR 16.96 |
Description: Fluid Thioglycollate Medium |
Fluid Thioglycollate Medium |
M009B-5KG |
EWC Diagnostics |
1 unit |
EUR 140.79 |
Description: Fluid Thioglycollate Medium |
Fluid Thioglycollate Medium (Thioglycoll |
M009-2.5KG |
EWC Diagnostics |
1 unit |
EUR 80.4 |
Description: Fluid Thioglycollate Medium (Thioglycoll |
Thioglycollate Medium Fluid |
M009-100G |
EWC Diagnostics |
1 unit |
EUR 9.83 |
Description: Thioglycollate Medium Fluid |
Thioglycollate Medium Fluid |
M009-10KG |
EWC Diagnostics |
1 unit |
EUR 279.29 |
Description: Thioglycollate Medium Fluid |
Thioglycollate Medium Fluid |
M009-500G |
EWC Diagnostics |
1 unit |
EUR 16.96 |
Description: Thioglycollate Medium Fluid |
Thioglycollate Medium Fluid |
M009-5KG |
EWC Diagnostics |
1 unit |
EUR 140.79 |
Description: Thioglycollate Medium Fluid |
Fluid Thioglycollate Medium (Thioglyco |
GM009-500G |
EWC Diagnostics |
1 unit |
EUR 19.53 |
Description: Fluid Thioglycollate Medium (Thioglyco |
Sterile Fluid Thioglycollate Medium |
LQ026-10X100ML |
EWC Diagnostics |
1 unit |
EUR 17.21 |
Description: Sterile Fluid Thioglycollate Medium |
Sterile Fluid Thioglycollate Medium |
LQ026IX-100X9ML |
EWC Diagnostics |
1 unit |
EUR 40.71 |
Description: Sterile Fluid Thioglycollate Medium |
Sterile Fluid Thioglycollate Medium |
LQ026IX-20X9ML |
EWC Diagnostics |
1 unit |
EUR 15.93 |
Description: Sterile Fluid Thioglycollate Medium |
Fluid Thioglycollate Medium w/ 0.05% |
LQ023-10X20ML |
EWC Diagnostics |
1 unit |
EUR 25.73 |
Description: Fluid Thioglycollate Medium w/ 0.05% |
Fluid Thioglycollate Medium w/ 0.05% |
LQ023A-10X70ML |
EWC Diagnostics |
1 unit |
EUR 28.11 |
Description: Fluid Thioglycollate Medium w/ 0.05% |
Fluid Thioglycollate Medium Solution |
40120793-1 |
Glycomatrix |
12x100 mL |
EUR 189.5 |
Fluid Thioglycollate Medium w/ HiVeg |
MV380-500G |
EWC Diagnostics |
1 unit |
EUR 29.31 |
Description: Fluid Thioglycollate Medium w/ HiVeg |
Fluid Thioglycollate HiCynth Medium |
MCD009-100G |
EWC Diagnostics |
1 unit |
EUR 11.3 |
Description: Fluid Thioglycollate HiCynth Medium |
Fluid Thioglycollate HiCynth Medium |
MCD009-500G |
EWC Diagnostics |
1 unit |
EUR 19.53 |
Description: Fluid Thioglycollate HiCynth Medium |
Sterile Fluid Thioglycollate Medium (Dou |
LQ026DW-10X100ML |
EWC Diagnostics |
1 unit |
EUR 35.29 |
Description: Sterile Fluid Thioglycollate Medium (Dou |
Fluid Thioglycollate Medium w/ 0.5% Soya |
M2082-500G |
EWC Diagnostics |
1 unit |
EUR 59.65 |
Description: Fluid Thioglycollate Medium w/ 0.5% Soya |
Fluid Thioglycollate Medium w/ 0.5% Soya |
LQ270C-10X100ML |
EWC Diagnostics |
1 unit |
EUR 23.17 |
Description: Fluid Thioglycollate Medium w/ 0.5% Soya |
Fluid Thioglycollate Medium w/ 0.5% Soya |
LQ288CV-10X100ML |
EWC Diagnostics |
1 unit |
EUR 34.39 |
Description: Fluid Thioglycollate Medium w/ 0.5% Soya |
Fluid Thioglycollate Medium w/ 0.1% Poly |
LQ315CRS-10X100ML |
EWC Diagnostics |
1 unit |
EUR 40.64 |
Description: Fluid Thioglycollate Medium w/ 0.1% Poly |
Thioglycollate HiVeg Medium Fluid |
MV009-100G |
EWC Diagnostics |
1 unit |
EUR 9.83 |
Description: Thioglycollate HiVeg Medium Fluid |
Thioglycollate HiVeg Medium Fluid |
MV009-500G |
EWC Diagnostics |
1 unit |
EUR 16.96 |
Description: Thioglycollate HiVeg Medium Fluid |
Fluid Thioglycollate Medium w/ 1% Tween |
LQ281C-10X100ML |
EWC Diagnostics |
1 unit |
EUR 50.4 |
Description: Fluid Thioglycollate Medium w/ 1% Tween |
Fluid Thioglycollate Medium w/ 4 IU/ML P |
LQ545CK-10X100ML |
EWC Diagnostics |
1 unit |
EUR 40.46 |
Description: Fluid Thioglycollate Medium w/ 4 IU/ML P |
Thioglycollate Medium, Linden |
M195-100G |
EWC Diagnostics |
1 unit |
EUR 12.27 |
Description: Thioglycollate Medium, Linden |
Thioglycollate Medium, Linden |
M195-500G |
EWC Diagnostics |
1 unit |
EUR 28.24 |
Description: Thioglycollate Medium, Linden |
Brewer Thioglycollate Medium |
M019-100G |
EWC Diagnostics |
1 unit |
EUR 12.27 |
Description: Brewer Thioglycollate Medium |
Brewer Thioglycollate Medium |
M019-500G |
EWC Diagnostics |
1 unit |
EUR 28.24 |
Description: Brewer Thioglycollate Medium |
Thioglycollate Medium w/ CaCO3 |
M765-500G |
EWC Diagnostics |
1 unit |
EUR 27.76 |
Description: Thioglycollate Medium w/ CaCO3 |
Alternative Thioglycollate Medium |
MM010-100G |
EWC Diagnostics |
1 unit |
EUR 9.83 |
Description: Alternative Thioglycollate Medium |
Alternative Thioglycollate Medium |
MM010-2.5KG |
EWC Diagnostics |
1 unit |
EUR 73.81 |
Description: Alternative Thioglycollate Medium |
Alternative Thioglycollate Medium |
MM010-500G |
EWC Diagnostics |
1 unit |
EUR 15.61 |
Description: Alternative Thioglycollate Medium |
Alternative Thioglycollate Medium |
MM010-5KG |
EWC Diagnostics |
1 unit |
EUR 129.15 |
Description: Alternative Thioglycollate Medium |
Alternative Thioglycollate Medium |
MU010-100G |
EWC Diagnostics |
1 unit |
EUR 9.83 |
Description: Alternative Thioglycollate Medium |
Alternative Thioglycollate Medium |
MU010-2.5KG |
EWC Diagnostics |
1 unit |
EUR 73.81 |
Description: Alternative Thioglycollate Medium |
Alternative Thioglycollate Medium |
MU010-500G |
EWC Diagnostics |
1 unit |
EUR 15.61 |
Description: Alternative Thioglycollate Medium |
Alternative Thioglycollate Medium |
MU010-5KG |
EWC Diagnostics |
1 unit |
EUR 129.15 |
Description: Alternative Thioglycollate Medium |
Alternative Thioglycollate Medium |
MU010G-500G |
EWC Diagnostics |
1 unit |
EUR 23.49 |
Description: Alternative Thioglycollate Medium |
Alternative Thioglycollate Medium |
MU010G-5KG |
EWC Diagnostics |
1 unit |
EUR 198.68 |
Description: Alternative Thioglycollate Medium |
Thioglycollate Medium w/ K Agar |
M430-500G |
EWC Diagnostics |
1 unit |
EUR 30.11 |
Description: Thioglycollate Medium w/ K Agar |
Campylo Thioglycollate Medium Base |
M908-500G |
EWC Diagnostics |
1 unit |
EUR 40.7 |
Description: Campylo Thioglycollate Medium Base |
*Thioglycollate Medium w/ Hemin and |
M979-500G |
EWC Diagnostics |
1 unit |
EUR 45.2 |
Description: *Thioglycollate Medium w/ Hemin and |
Anaerobic Thioglycollate Medium Base |
M1616-500G |
EWC Diagnostics |
1 unit |
EUR 38.04 |
Description: Anaerobic Thioglycollate Medium Base |
Brewer Thioglycollate Medium, Modified |
M195A-100G |
EWC Diagnostics |
1 unit |
EUR 12.27 |
Description: Brewer Thioglycollate Medium, Modified |
Brewer Thioglycollate Medium, Modified |
M195A-500G |
EWC Diagnostics |
1 unit |
EUR 28.24 |
Description: Brewer Thioglycollate Medium, Modified |
Alternative Thioglycollate Medium ?(NIH |
GM010-500G |
EWC Diagnostics |
1 unit |
EUR 19.53 |
Description: Alternative Thioglycollate Medium ?(NIH |
Thioglycollate Medium w/o Dextrose |
M190-500G |
EWC Diagnostics |
1 unit |
EUR 30.11 |
Description: Thioglycollate Medium w/o Dextrose |
Fluid Thioglycollate w/ HM Peptone B |
M380-500G |
EWC Diagnostics |
1 unit |
EUR 29.31 |
Description: Fluid Thioglycollate w/ HM Peptone B |
Alternative Thioglycollate Medium, Ster |
MU010G-2.5KG |
EWC Diagnostics |
1 unit |
EUR 110.34 |
Description: Alternative Thioglycollate Medium, Ster |
Thioglycollate Medium w/o Indicator |
M191-500G |
EWC Diagnostics |
1 unit |
EUR 32.6 |
Description: Thioglycollate Medium w/o Indicator |
Alternative Thioglycollate Medium, Steri |
M010G-2.5KG |
EWC Diagnostics |
1 unit |
EUR 103.95 |
Description: Alternative Thioglycollate Medium, Steri |
Alternative Thioglycollate Medium, Steri |
M010G-500G |
EWC Diagnostics |
1 unit |
EUR 22.42 |
Description: Alternative Thioglycollate Medium, Steri |
Alternative Thioglycollate Medium, Steri |
M010G-5KG |
EWC Diagnostics |
1 unit |
EUR 186.93 |
Description: Alternative Thioglycollate Medium, Steri |
Brewer Thioglycollate HiVeg Medium |
MV019-100G |
EWC Diagnostics |
1 unit |
EUR 12.27 |
Description: Brewer Thioglycollate HiVeg Medium |
Brewer Thioglycollate HiVeg Medium |
MV019-500G |
EWC Diagnostics |
1 unit |
EUR 28.24 |
Description: Brewer Thioglycollate HiVeg Medium |
Brewer Thioglycollate HiVeg Medium, |
MV195-100G |
EWC Diagnostics |
1 unit |
EUR 12.27 |
Description: Brewer Thioglycollate HiVeg Medium, |
Brewer Thioglycollate HiVeg Medium, |
MV195-500G |
EWC Diagnostics |
1 unit |
EUR 28.24 |
Description: Brewer Thioglycollate HiVeg Medium, |
Thioglycollate HiVeg Medium w/o |
MV191-500G |
EWC Diagnostics |
1 unit |
EUR 32.6 |
Description: Thioglycollate HiVeg Medium w/o |
Alternative Thioglycollate Medium (NIH T |
M010-2.5KG |
EWC Diagnostics |
1 unit |
EUR 80.4 |
Description: Alternative Thioglycollate Medium (NIH T |
Thioglycollate Medium w/o Dextrose and |
M1614-500G |
EWC Diagnostics |
1 unit |
EUR 33.1 |
Description: Thioglycollate Medium w/o Dextrose and |
Thioglycollate Medium with Hemin and Vit |
LQ319V-25X5ML |
EWC Diagnostics |
1 unit |
EUR 16.24 |
Description: Thioglycollate Medium with Hemin and Vit |
Thioglycollate Medium with Hemin and Vit |
LQ319V-50X5ML |
EWC Diagnostics |
1 unit |
EUR 29.18 |
Description: Thioglycollate Medium with Hemin and Vit |
Thioglycollate Medium with Hemin and Vit |
LQ319VIII-25X8ML |
EWC Diagnostics |
1 unit |
EUR 17.82 |
Description: Thioglycollate Medium with Hemin and Vit |
Thioglycollate Medium with Hemin and Vit |
LQ319VIII-50X8ML |
EWC Diagnostics |
1 unit |
EUR 32.07 |
Description: Thioglycollate Medium with Hemin and Vit |
Campylo Thioglycollate HiVeg Medium Base |
MV908-500G |
EWC Diagnostics |
1 unit |
EUR 40.7 |
Description: Campylo Thioglycollate HiVeg Medium Base |
Alternative Thioglycollate HiVeg™ Medium |
MV010G-5KG |
EWC Diagnostics |
1 unit |
EUR 181.49 |
Description: Alternative Thioglycollate HiVeg™ Medium |
Sterile Alternative Thioglycollate Mediu |
LQ028-10X100ML |
EWC Diagnostics |
1 unit |
EUR 24.33 |
Description: Sterile Alternative Thioglycollate Mediu |
Alternative Thioglycollate HiCynth Mediu |
MCD010-100G |
EWC Diagnostics |
1 unit |
EUR 11.3 |
Description: Alternative Thioglycollate HiCynth Mediu |
Alternative Thioglycollate HiCynth Mediu |
MCD010-500G |
EWC Diagnostics |
1 unit |
EUR 19.53 |
Description: Alternative Thioglycollate HiCynth Mediu |
Alternative Thioglycollate HiVeg™ Mediu |
MV010-2.5KG |
EWC Diagnostics |
1 unit |
EUR 80.4 |
Description: Alternative Thioglycollate HiVeg™ Mediu |
Alternative Thioglycollate HiVeg™ Mediu |
MV010-5KG |
EWC Diagnostics |
1 unit |
EUR 140.79 |
Description: Alternative Thioglycollate HiVeg™ Mediu |
Alternative Thioglycollate HiVeg™ Mediu |
MV010G-2.5KG |
EWC Diagnostics |
1 unit |
EUR 100.96 |
Description: Alternative Thioglycollate HiVeg™ Mediu |
Alternative Thioglycollate HiVeg™ Mediu |
MV010G-500G |
EWC Diagnostics |
1 unit |
EUR 22.42 |
Description: Alternative Thioglycollate HiVeg™ Mediu |
Thioglycollate Agar |
M608-500G |
EWC Diagnostics |
1 unit |
EUR 42.71 |
Description: Thioglycollate Agar |
Thioglycollate Broth |
LQ007-10X20ML |
EWC Diagnostics |
1 unit |
EUR 16.75 |
Description: Thioglycollate Broth |
Thioglycollate Broth |
LQ007A-10X70ML |
EWC Diagnostics |
1 unit |
EUR 20.69 |
Description: Thioglycollate Broth |
Thioglycollate HiVeg Agar |
MV608-500G |
EWC Diagnostics |
1 unit |
EUR 42.71 |
Description: Thioglycollate HiVeg Agar |
Enriched Thioglycollate Broth |
M738-500G |
EWC Diagnostics |
1 unit |
EUR 39.31 |
Description: Enriched Thioglycollate Broth |
Thioglycollate Broth, Alternative |
M010-100G |
EWC Diagnostics |
1 unit |
EUR 9.83 |
Description: Thioglycollate Broth, Alternative |
Thioglycollate Broth, Alternative |
M010-500G |
EWC Diagnostics |
1 unit |
EUR 16.96 |
Description: Thioglycollate Broth, Alternative |
Materials and methods: We used 3D breast tumour models to determine morphological alterations, and the levels of secreted transforming growth factor-β (TGFβ) and induced cytokines. 3D luminal phenotype models and basal phenotype models were generated by culturing NK cells and CD4+CD25+ TREG cells with MCF-7 cells and MDA-MB-231 cells respectively, in growth factor-reduced Matrigel. TGFβ was qualitatively assessed by immunolocalisation and cytokine data from culture supernatant was acquired with a multiplex cytokine assay. Traditional statistical analysis and principal component analysis were employed to unravel the cytokine response.
Results and conclusion: We identified that an interleukin-6 (IL6)-chemokine axis associated with TGFβ is primarily responsible for differences detected between breast cancer models, with luminal and basal phenotype tumours responding differentially to immune mediation. Identified cytokines are implicated in facilitating tumour cell subversion of immune cell function to promote an invasive phenotype. Moreover, the disruption of the extracellular matrix and failure to form well-differentiated tumour masses/networks is indicative of enhanced malignancy. Tumour cells are implicated in promoting a pro-inflammatory microenvironment to attenuate NK cell function and in inducing a pro-tumorigenic profile that is facilitated by TREG lymphocytes.
Growing human trophoblasts in vitro: a review of the media commonly used in trophoblast cell culture
Trophoblasts are unique epithelial cells found only in the placenta. It has been possible to isolate and maintain human trophoblasts in in vitro culture for many decades. During this period there have been a vast array of media and supplements reported for trophoblast culture and often the reasons for using the media and specific supplements employed in any given laboratory have been lost in the ‘mists of time’. After a gradual development over many years this field has recently changed, with the publication of several reports of the isolation, growth and differentiation of human trophoblast stem or stem-like cells.
This advance was made largely because of a greater understanding of the molecular pathways that control human trophoblasts and availability of media supplements that can be used to manipulate those pathways. We have searched the literature and here summarise many of the different media and supplements and describe how and why they were developed and are used to culture human trophoblasts.
Establishment of Conditions for Long-Term Maintenance of Primary Embryonic Cell Cultures from Olive Flounder Paralichthys olivaceus
Primary cell culture is a sufficient method frequently used to study the cellular properties and mechanisms of isolated cells in a controlled environment. In this study, an embryonic cell line (FGBC8) derived from the blastula stages of embryos of olive flounder Paralichthys olivaceus was developed. Furthermore, conditions for optimal long-term maintenance of this primary embryonic cell culture were investigated. Morphologically, FGBC8 cells were composed primarily of epithelial-like cells. FGBC8 cells were subcultured for >160 passages over ~830 days.
The doubling time of FGBC8 cells was 73.8 h, and the modal diploid chromosome number was 48. FGBC8 cells transfected with green fluorescence protein (GFP)-expression plasmid exhibited a strong signal 48 h after transfection. Consequently, we demonstrated that fish serum is a crucial supplement for the long-term survival and maintenance of comparable morphology in these primary embryonic cells. Our results can be used as a guide for primary embryonic cell cultures for other fish species and may be useful for cell biotechnological applications.
Neurogranin precursor Antibody |
abx432061-200ul |
Abbexa |
200 ul |
EUR 460.8 |
|
Neurogranin precursor Antibody |
abx432061-100g |
Abbexa |
100 µg |
EUR 387.5 |
Porcine Parvovirus Antibody Elisa Test Kit |
767-LSY-30009 |
Gentaur Genprice |
192 Wells/kit |
EUR 382 |
Precursor Neurotrophin-3 Protein |
20-abx262357 |
Abbexa |
-
Ask for price
-
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-
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Neurogranin precursor Antibody (Biotin) |
abx433025-200ul |
Abbexa |
200 ul |
EUR 460.8 |
|
Neurogranin precursor Antibody (Biotin) |
abx433025-200g |
Abbexa |
200 µg |
EUR 387.5 |
Recombinant human Neurofilament medium polypeptide |
P1623 |
FN Test |
100ug |
Ask for price |
|
Description: Recombinant protein for human Neurofilament medium polypeptide |
N-Terminal Brain Natriuretic Peptide Precursor (NT-proBNP) Rapid Test Kit |
abx092227-100l |
Abbexa |
100 µl |
EUR 1000 |
N-Terminal Brain Natriuretic Peptide Precursor (NT-proBNP) Rapid Test Kit |
abx092227-1ml |
Abbexa |
1 ml |
EUR 12000 |
N-Terminal Brain Natriuretic Peptide Precursor (NT-proBNP) Rapid Test Kit |
abx092227-200l |
Abbexa |
200 µl |
EUR 3500 |
Ovine Brown Adipocyte Precursor Cell Line |
T6311 |
ABM |
1x10^6 cells / 1.0 ml |
EUR 3950 |
Recombinant Human Precursor Neurotrophin-3 |
7-01297 |
CHI Scientific |
2µg |
Ask for price |
Recombinant Human Precursor Neurotrophin-3 |
7-01298 |
CHI Scientific |
10µg |
Ask for price |
Recombinant Human Precursor Neurotrophin-3 |
7-01299 |
CHI Scientific |
1mg |
Ask for price |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (Recombinant) |
20-abx073292 |
Abbexa |
-
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Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) Antibody |
20-abx322117 |
Abbexa |
-
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-
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Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) Antibody |
20-abx322118 |
Abbexa |
-
Ask for price
-
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|
|
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
20-abx322208 |
Abbexa |
-
Ask for price
-
Ask for price
|
|
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
20-abx322255 |
Abbexa |
-
Ask for price
-
Ask for price
|
|
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) Antibody |
abx433019-200ul |
Abbexa |
200 ul |
EUR 460.8 |
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
abx431451-200ul |
Abbexa |
200 ul |
EUR 460.8 |
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
20-abx330023 |
Abbexa |
-
Ask for price
-
Ask for price
|
|
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx330644-100ul |
Abbexa |
100 ul |
EUR 510 |
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
20-abx339618 |
Abbexa |
-
Ask for price
-
Ask for price
|
|
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
20-abx301891 |
Abbexa |
-
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-
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-
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-
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-
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|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
20-abx126230 |
Abbexa |
-
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-
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-
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-
Ask for price
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) Antibody |
abx235644-100ug |
Abbexa |
100 ug |
EUR 577.2 |
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx235647-100ug |
Abbexa |
100 ug |
EUR 577.2 |
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
20-abx136138 |
Abbexa |
-
Ask for price
-
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-
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|
|
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) Antibody |
abx122112-100ug |
Abbexa |
100 ug |
EUR 469.2 |
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
abx122676-100ug |
Abbexa |
100 ug |
EUR 469.2 |
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) Antibody |
20-abx114057 |
Abbexa |
-
Ask for price
-
Ask for price
|
|
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
20-abx114060 |
Abbexa |
-
Ask for price
-
Ask for price
|
|
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) Antibody |
20-abx003925 |
Abbexa |
-
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-
Ask for price
-
Ask for price
-
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|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
20-abx008944 |
Abbexa |
-
Ask for price
-
Ask for price
-
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|
|
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx012321-100ug |
Abbexa |
100 ug |
EUR 493.2 |
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
20-abx000774 |
Abbexa |
-
Ask for price
-
Ask for price
|
|
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
20-abx001077 |
Abbexa |
-
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-
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-
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-
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|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
20-abx001962 |
Abbexa |
-
Ask for price
-
Ask for price
-
Ask for price
-
Ask for price
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
20-abx013221 |
Abbexa |
-
Ask for price
-
Ask for price
-
Ask for price
-
Ask for price
|
- 100 ug
- 10 ug
- 200 ug
- 300 µg
|
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx015935-100ug |
Abbexa |
100 ug |
EUR 493.2 |
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx026692-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx026692-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
abx028164-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
abx028164-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
abx001962-100l |
Abbexa |
100 µl |
EUR 400 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
abx001962-20l |
Abbexa |
20 µl |
EUR 175 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
abx001962-50l |
Abbexa |
50 µl |
EUR 275 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) Antibody |
abx003925-100l |
Abbexa |
100 µl |
EUR 400 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) Antibody |
abx003925-20l |
Abbexa |
20 µl |
EUR 175 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) Antibody |
abx003925-50l |
Abbexa |
50 µl |
EUR 275 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx013221-100g |
Abbexa |
100 µg |
EUR 237.5 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx013221-10g |
Abbexa |
10 µg |
EUR 43.75 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx013221-200g |
Abbexa |
200 µg |
EUR 312.5 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx001077-100l |
Abbexa |
100 µl |
EUR 400 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx001077-20l |
Abbexa |
20 µl |
EUR 175 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx001077-50l |
Abbexa |
50 µl |
EUR 275 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx012321-100g |
Abbexa |
100 µg |
Ask for price |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx012321-10g |
Abbexa |
10 µg |
EUR 362.5 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx012321-200g |
Abbexa |
200 µg |
Ask for price |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx015935-1mg |
Abbexa |
1 mg |
EUR 362.5 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) Antibody |
abx235644-100g |
Abbexa |
100 µg |
EUR 350 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx235647-100g |
Abbexa |
100 µg |
EUR 350 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
abx301891-100g |
Abbexa |
100 µg |
EUR 362.5 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
abx301891-20g |
Abbexa |
20 µg |
EUR 162.5 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
abx301891-50g |
Abbexa |
50 µg |
EUR 250 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
abx126230-100l |
Abbexa |
100 µl |
EUR 175 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
abx126230-1ml |
Abbexa |
1 ml |
EUR 387.5 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
abx126230-200l |
Abbexa |
200 µl |
EUR 275 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
abx122676-100l |
Abbexa |
100 µl |
Ask for price |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
abx122676-20l |
Abbexa |
20 µl |
EUR 337.5 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
abx122676-50l |
Abbexa |
50 µl |
Ask for price |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) Antibody |
abx122112-100l |
Abbexa |
100 µl |
Ask for price |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) Antibody |
abx122112-20l |
Abbexa |
20 µl |
EUR 337.5 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) Antibody |
abx122112-50l |
Abbexa |
50 µl |
Ask for price |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) Antibody |
abx114057-100l |
Abbexa |
100 µl |
EUR 612.5 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx114060-100l |
Abbexa |
100 µl |
EUR 612.5 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx026692-400l |
Abbexa |
400 µl |
EUR 518.75 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
abx028164-400l |
Abbexa |
400 µl |
EUR 518.75 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
abx339618-100l |
Abbexa |
100 µl |
EUR 350 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
abx339618-50l |
Abbexa |
50 µl |
EUR 250 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) Antibody |
abx322117-100g |
Abbexa |
100 µg |
EUR 350 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) Antibody |
abx322117-50g |
Abbexa |
50 µg |
EUR 250 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) Antibody |
abx322118-100g |
Abbexa |
100 µg |
EUR 350 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) Antibody |
abx322118-50g |
Abbexa |
50 µg |
EUR 250 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx322208-100g |
Abbexa |
100 µg |
EUR 350 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx322208-50g |
Abbexa |
50 µg |
EUR 237.5 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx322255-100g |
Abbexa |
100 µg |
EUR 350 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx322255-50g |
Abbexa |
50 µg |
EUR 237.5 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) Antibody |
abx431451-200l |
Abbexa |
200 µl |
EUR 387.5 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) Antibody |
abx433019-200g |
Abbexa |
200 µg |
EUR 387.5 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) Antibody |
abx376408-96tests |
Abbexa |
96 tests |
EUR 225 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx330023-100g |
Abbexa |
100 µg |
Ask for price |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx330023-20g |
Abbexa |
20 µg |
EUR 187.5 |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antibody |
abx330023-50g |
Abbexa |
50 µg |
EUR 250 |
Sources
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