Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System

November 12, 2020
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eaaci2015

Effects of 660-nm and 810-nm Low-Power Diode Laser on Proliferation and Invasion of Oral Cancer Cells in Cell Culture Media

 

In the present study, the effects of 660- and 810-nm diode laser on the proliferation and invasion of cancer cells were investigated. Sixteen plates of oral cancer cells originated from tongue SCC were irradiated with diode laser at 660-nm (40 and 80 mW) and 810-nm (100 and 200 mW) with the energy density of 4 J/cm2. One plate received no irradiation (the control). Irradiation was performed in four times (0,24,72 and 168 hours). Cell proliferation was measured by MTT assay.
The Ki67 and vascular endothelial growth factor (VEGF) markers were examined by real-time polymerase chain reaction (RT-PCR). Cyclin D1, E-cadherin, β-catenin, matrix metalloproteinase-9 (MMP-9; flow cytometry), were also evaluated. Proliferation was lower in the irradiated groups. This result was significant for all groups at the 24-hour. The percentages of Cyclin D1and MMP-9 were higher in 810nm groups, β-catenin and E-cadherin were higher in 660 nm groups, VEGF marker was significantly lower in 810 nm/200 mW group and Ki67 marker was no difference between the groups. According to the results of this study, laser irradiation at 0- and 24-h resulted in a significant inhibitory effect on cell proliferation especially in 660-nm / 80-mW and 810-nm / 200-mW. Further studies are needed in this respect.

A Biologist’s Guide to Traction Force Microscopy Using Polydimethylsiloxane Substrate for Two-Dimensional Cell Cultures

 

Cellular traction forces influence epithelial behavior, including wound healing and cell extrusion. Here, we describe a simple in vitro traction force microscopy (TFM) protocol using ECM protein-coated polydimethylsiloxane substrate and widefield fluorescence microscopy. We include detailed steps for analysis so readers can obtain traction forces to study the mechanobiology of epithelial cells. We also provide guidelines on when to adopt another common class of TFM protocols based on polyacrylamide hydrogels. For complete details on the use and execution of this protocol, please refer to Saw et al. (2017) and Teo et al. (2020).

Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System

 

Melanocytes, derived from neural crest cells, are involved in melanin production. This protocol describes a method to generate induced melanocytes (iMelanocytes) from human induced pluripotent stem cells (iPSCs) using a suspension culture system, which considerably improves the differentiation efficiency. The most critical parts of this protocol are the selection of a reliable iPSC line with strong potential to differentiate into melanocytes and their stemness maintenance. For complete information on the use and generation of this protocol, please refer to our Cell Reports article, Liu el al. (2019).

Breast Cancer Cells Induce a Pro-inflammatory Response to Mitigate Immune Mediation in a 3D Culture Model

Background: Growth factors and cytokines mediate complex interactions between cells within the breast tumour microenvironment. In advanced cancer, an excess of regulatory T (TREG) lymphocytes and lack of natural killer (NK) cells in tumour-infiltrating lymphocyte populations may reflect a shift to pro-tumorigenic adaptive immune mechanisms. To facilitate targeted assessment of the interactions between tumour and immune cells ex vivo, three-dimensional (3D) culture systems are able to better recapitulate the in vivo microenvironment, recreating the anatomy of tumours.
eaaci2015

eaaci2015

THIOGLYCOLLATE MEDIUM, BREWER
T20-118-500g 500 g
EUR 128

SODIUM THIOGLYCOLLATE
S19-136-10kg 10 kg Ask for price

SODIUM THIOGLYCOLLATE
S19-136-2Kg 2 Kg Ask for price

SODIUM THIOGLYCOLLATE
S19-136-500g 500 g Ask for price

ALT. THIOGLYCOLLATE MED.
A01-103-10kg 10 kg Ask for price

ALT. THIOGLYCOLLATE MED.
A01-103-2Kg 2 Kg Ask for price

ALT. THIOGLYCOLLATE MED.
A01-103-500g 500 g Ask for price

Bouin's Fluid
BNF125 125 ml
EUR 63

Bouin's Fluid
BNF3800 1 Gal.
EUR 178

Bouin's Fluid
BNF500 500 ml
EUR 80

Bouin's Fluid
BNF999 1000 ml
EUR 98

Michel's Transport Fluid
MTF-10000 10 L
EUR 762

Michel's Transport Fluid
MTF-20000 20 L
EUR 1340

Michel's Transport Fluid
MTF500 500 ml
EUR 98

Michel's Transport Fluid
MTF999 1000 ml
EUR 127

SOF Synthetic Oviduct Fluid.
IVL05-100ML 100 ml
EUR 78
  • Product line: Animal Cell Culture Media
  • Product family: Bovine IVF Media
Description: Synthetic Oviduct Fluid.

SOF Synthetic Oviduct Fluid.
IVL05-6X100ML 6 x 100 ml
EUR 151
  • Product line: Animal Cell Culture Media
  • Product family: Bovine IVF Media
Description: Synthetic Oviduct Fluid.

HE4, Human ascites fluid
P1438-100
EUR 620

HE4, Human ascites fluid
P1438-500
EUR 2839

CA-125, Human Ascites Fluid
P1446-10
EUR 294

Exosome Isolation kit (forHuman Body Fluid)
P121 NULL
EUR 0

Exosome Isolation kit (forHuman Body Fluid)
P121L NULL
EUR 0

Exosome Isolation kit (forHuman Body Fluid)
P121S NULL
EUR 0

Cancer Antigen 125 Antigen (Ascites Fluid)
VAng-Cr3870-10kU 10 kU
EUR 944
Description: Cancer Antigen 125 Antigen, Host/Source: Ascites Fluid. The purity is ≥ 95% by SDS-PAGE.

Mounting Medium
4300 3 ml
EUR 180.5
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein.

VALI Medium
450 500 ml
EUR 265

BEGM Medium
451 500 ml
EUR 152

A Medium
DJ1018 100g
EUR 84.8
  • Product category: Culture Media/Medium

DC MEDIUM
D04-117-10kg 10 kg
EUR 1579

DC MEDIUM
D04-117-2kg 2kg
EUR 382

DC MEDIUM
D04-117-500g 500 g
EUR 140

EC MEDIUM
E05-100-10kg 10 kg
EUR 814

EC MEDIUM
E05-100-2Kg 2 Kg
EUR 216

EC MEDIUM
E05-100-500g 500 g
EUR 95

Advanced Medium
C0003-04 RT 500 mL Bottle
EUR 103

Medium 199
C0012-01 RT 500 mL Bottle
EUR 105

COLIFORM MEDIUM
C03-127-10kg 10 kg
EUR 1324

COLIFORM MEDIUM
C03-127-2kg 2kg
EUR 327

COLIFORM MEDIUM
C03-127-500g 500 g
EUR 125

Heller's Medium
CP014-010 10X1L
EUR 99

Heller's Medium
CP014-500 50L
EUR 126

Hoagland's Medium
CP015-010 10X1L
EUR 99

Hoagland's Medium
CP015-500 50L
EUR 126

HLP MEDIUM
H08-107-10kg 10 kg
EUR 2278

HLP MEDIUM
H08-107-2Kg 2 Kg
EUR 534

HLP MEDIUM
H08-107-500g 500 g
EUR 182

NeuroProgenitor Medium
NM42400 125 ml
EUR 304

M9 Medium
SD7024 250g
EUR 71.75
  • Product category: Culture Media/Medium

M9CA Medium
SD7025 250g
EUR 71.75
  • Product category: Culture Media/Medium

YM Medium
SD7031 250g
EUR 70.45
  • Product category: Culture Media/Medium

TYGPN Medium
SD7032 250g
EUR 70.01
  • Product category: Culture Media/Medium

M63 Medium
SD7033 250g
EUR 71.75
  • Product category: Culture Media/Medium

SIM MEDIUM
S19-110-10kg 10 kg
EUR 1021

SIM MEDIUM
S19-110-2kg 2kg
EUR 261

SIM MEDIUM
S19-110-500g 500 g
EUR 107

SOB MEDIUM
S19-124-10kg 10 kg
EUR 965

SOB MEDIUM
S19-124-2kg 2kg
EUR 249

SOB MEDIUM
S19-124-500g 500 g
EUR 104

Yellow fever virus infectious culture fluid(17D)
DAGF-040 1ml
EUR 645

Immortalized Human Amniotic Fluid Stem Cells - SV40
T0903 1x106 cells / 1.0 ml Ask for price

Insect Cell Medium: TNM-FH Insect Culture Medium
ABP-MED-10001 1 liter Ask for price
    • Product line: Cell Culture Reagents
    • Brand:

Insect Cell Medium: Serum-Free Insect Culture Medium
ABP-MED-10002 1 liter Ask for price
    • Product line: Cell Culture Reagents
    • Brand:

Insect Cell Medium: Grace?s Insect Medium (Unsupplemented)
ABP-MED-10004 1 liter Ask for price
    • Product line: Cell Culture Reagents
    • Brand:

EverBrite Mounting Medium
23001 10mL
EUR 178
Description: Minimum order quantity: 1 unit of 10mL

Recovery Medium - 6x12mL
1711 4/PK
EUR 128

SuperMOUNT? Mounting Medium
1211-20
EUR 201

Aqueous Mounting Medium
AR1018 10mL X5 (Enough for 800-1200 assays)
EUR 80

Antifade Mounting Medium
AR1109 10mL (for 1000 assays)
EUR 65

H Medium Broth
DJ1019 100g
EUR 60.88
  • Product category: Culture Media/Broth

EC MEDIUM, MODIFIED
E05-108-10kg 10 kg
EUR 858

EC MEDIUM, MODIFIED
E05-108-2Kg 2 Kg
EUR 226

EC MEDIUM, MODIFIED
E05-108-500g 500 g
EUR 98

A-1 MEDIUM
A01-116-10kg 10 kg
EUR 1034

A-1 MEDIUM
A01-116-2Kg 2 Kg
EUR 264

A-1 MEDIUM
A01-116-500g 500 g
EUR 108

RPMI 1640 Medium
abx098879-500ml 500 ml
EUR 175
  • Shipped within 5-10 working days.

MCDB 131 Medium
CM034-050 500ml
EUR 85

MCDB 131 Medium
CM034-300 6x500ml
EUR 165

MCDB 131 Medium
CM034-310 10x500ml
EUR 219

MCDB 131 Medium
CM034-320 20x500ml
EUR 340

MCDB 131 Medium
CM034-350 50x500ml
EUR 585

Williams' Medium E
CM063-050 500ml
EUR 91

Williams' Medium E
CM063-300 6x500ml
EUR 266

Williams' Medium E
CM063-310 10x500ml
EUR 313

Williams' Medium E
CM063-320 20x500ml
EUR 604

Williams' Medium E
CM063-350 50x500ml
EUR 975

AddexBio Cryopreservation Medium
C0002-20mL RT vial
EUR 104

CARY & BLAIR MEDIUM
C03-131-10kg 10 kg
EUR 2185

CARY & BLAIR MEDIUM
C03-131-2Kg 2 Kg
EUR 514

CARY & BLAIR MEDIUM
C03-131-500g 500 g
EUR 176

Cereal Crops Medium
CP003-005 5L
EUR 113

Cereal Crops Medium
CP003-010 10X1L
EUR 169

Kao Michayluk Medium
CP017-010 10X1L
EUR 99

Kao Michayluk Medium
CP017-500 50L
EUR 126

Orchid Maintenance Medium
CP037-010 10X1L
EUR 109

Orchid Maintenance Medium
CP037-500 50L
EUR 138

Woody Plants Medium
CP040-010 10X1L
EUR 101

Woody Plants Medium
CP040-500 50L
EUR 126

Hepatocyte Maintenance Medium
HM42600 100 ml
EUR 409

Neuron Growth Medium
HNM001 500 ml
EUR 331

Agarose, medium EEO
GE4750-100G 100 g
EUR 166

Agarose, medium EEO
GE4750-500G 500 g
EUR 429

FluoreGuard Mounting Medium
FMM030 30 ml
EUR 96

FluoreGuard Mounting Medium
FMM060 60 ml
EUR 116

FluoreGuard Mounting Medium
FMM500 500 ml
EUR 327

FluoreGuard Mounting Medium
FMM999 1000 ml
EUR 530

OF BASAL MEDIUM
O15-100-10kg 10 kg Ask for price

OF BASAL MEDIUM
O15-100-2kg 2kg Ask for price

OF BASAL MEDIUM
O15-100-500g 500 g Ask for price

MOTILITY TEST MEDIUM
M13-121-10kg 10 kg
EUR 1528
Materials and methods: We used 3D breast tumour models to determine morphological alterations, and the levels of secreted transforming growth factor-β (TGFβ) and induced cytokines. 3D luminal phenotype models and basal phenotype models were generated by culturing NK cells and CD4+CD25+ TREG cells with MCF-7 cells and MDA-MB-231 cells respectively, in growth factor-reduced Matrigel. TGFβ was qualitatively assessed by immunolocalisation and cytokine data from culture supernatant was acquired with a multiplex cytokine assay. Traditional statistical analysis and principal component analysis were employed to unravel the cytokine response.
Results and conclusion: We identified that an interleukin-6 (IL6)-chemokine axis associated with TGFβ is primarily responsible for differences detected between breast cancer models, with luminal and basal phenotype tumours responding differentially to immune mediation. Identified cytokines are implicated in facilitating tumour cell subversion of immune cell function to promote an invasive phenotype. Moreover, the disruption of the extracellular matrix and failure to form well-differentiated tumour masses/networks is indicative of enhanced malignancy. Tumour cells are implicated in promoting a pro-inflammatory microenvironment to attenuate NK cell function and in inducing a pro-tumorigenic profile that is facilitated by TREG lymphocytes.

Growing human trophoblasts in vitro: a review of the media commonly used in trophoblast cell culture

Trophoblasts are unique epithelial cells found only in the placenta. It has been possible to isolate and maintain human trophoblasts in in vitro culture for many decades. During this period there have been a vast array of media and supplements reported for trophoblast culture and often the reasons for using the media and specific supplements employed in any given laboratory have been lost in the ‘mists of time’. After a gradual development over many years this field has recently changed, with the publication of several reports of the isolation, growth and differentiation of human trophoblast stem or stem-like cells.
This advance was made largely because of a greater understanding of the molecular pathways that control human trophoblasts and availability of media supplements that can be used to manipulate those pathways. We have searched the literature and here summarise many of the different media and supplements and describe how and why they were developed and are used to culture human trophoblasts.

Establishment of Conditions for Long-Term Maintenance of Primary Embryonic Cell Cultures from Olive Flounder Paralichthys olivaceus

Primary cell culture is a sufficient method frequently used to study the cellular properties and mechanisms of isolated cells in a controlled environment. In this study, an embryonic cell line (FGBC8) derived from the blastula stages of embryos of olive flounder Paralichthys olivaceus was developed. Furthermore, conditions for optimal long-term maintenance of this primary embryonic cell culture were investigated. Morphologically, FGBC8 cells were composed primarily of epithelial-like cells. FGBC8 cells were subcultured for >160 passages over ~830 days.
The doubling time of FGBC8 cells was 73.8 h, and the modal diploid chromosome number was 48. FGBC8 cells transfected with green fluorescence protein (GFP)-expression plasmid exhibited a strong signal 48 h after transfection. Consequently, we demonstrated that fish serum is a crucial supplement for the long-term survival and maintenance of comparable morphology in these primary embryonic cells. Our results can be used as a guide for primary embryonic cell cultures for other fish species and may be useful for cell biotechnological applications.
Immortalized Human Dopaminergic Neuronal Precursor Cells (LUHMES)
T0284 1x106 cells / 1.0 ml
EUR 340
Recombinant human Isoform 3 of Neuronal cell adhesion molecule
P2740 100ug Ask for price
  • Uniprot ID: Q92823-3
  • Reconstitution: Metal affinity chromatography on Fn Super Capacity Column (Nickel)
Description: Recombinant protein for human Isoform 3 of Neuronal cell adhesion molecule
Recombinant human Neurofilament medium polypeptide
P1623 100ug Ask for price
  • Uniprot ID: P07197
  • Reconstitution: Metal affinity chromatography on Fn Super Capacity Column (Nickel)
Description: Recombinant protein for human Neurofilament medium polypeptide
Insect Cell Medium: TNM-FH Insect Culture Medium
ABP-MED-10001 1 liter Ask for price
    • Product line: Cell Culture Reagents
    • Brand:
Insect Cell Medium: Serum-Free Insect Culture Medium
ABP-MED-10002 1 liter Ask for price
    • Product line: Cell Culture Reagents
    • Brand:
Insect Cell Medium: Grace?s Insect Medium (Unsupplemented)
ABP-MED-10004 1 liter Ask for price
    • Product line: Cell Culture Reagents
    • Brand:
Human Epithelial Cell Medium
ECM001 500 mL
EUR 323
Stellate Cell Medium/kit
C0009-01 RT 500 mL Bottle
EUR 227
Cervical Epithalial cell medium
C0011-01 RT 500 mL Bottle
EUR 239
Accu-Tell COVID-19 IgG/IgM Rapid Test
GEN-B352-20tests 20 tests
EUR 236
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.
Accu-Tell COVID-19 IgG/IgM Rapid Test
GEN-B352-40tests 40 tests
EUR 321
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.
Human MEP Cell Culture Medium (human megakaryocyte–erythroid progenitor cell culture medium)
P770 NULL
EUR 0
Gryphon Packaging Cell Selection Medium
ABP-TC-PHSM050 50 ml Ask for price
    • Product line: Gryphon Retrovirus
    • Brand: Packaging System
Description: PRODUCT LINE DISCONTINUED. Please contact us.
Human CD34+ Cell Culture Medium
P771 NULL
EUR 0
PK15 Cell Serum-Free Medium
VCum-Lsx0007 1 L
EUR 758
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.
CHO Cell Serum-Free Medium
VCum-Lsx0025 1L
EUR 635
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).
HEK Cell Serum-Free Medium
VCum-Lsx0027 1L
EUR 635
Description: A serum-free medium that is ideal for suspension culture of HEK cell lines.
MDBK Cell Serum-Free Medium
VCum-Lsx0029 1L
EUR 682
Description: Used in serum-free suspension MDBK cell line culture for bovine virus diarrhea or bovine rhinotracheitis vaccine production.
Vero Cell Serum-Free Medium
VCum-Lsx0031 1 L
EUR 758
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.
ICA (Anti-insulin cell antibody) ELISA test
26 96T/Box Ask for price
  • Area of application: Hormone testing
Description: ELISA based test for quantitative detection of ICA (Anti-insulin cell antibody)
DNASE TEST AGAR
D04-101-10kg 10 kg Ask for price
DNASE TEST AGAR
D04-101-2Kg 2 Kg Ask for price
DNASE TEST AGAR
D04-101-500g 500 g Ask for price
DERMATOPHYTE TEST AGAR
D04-107-10kg 10 kg
EUR 849
DERMATOPHYTE TEST AGAR
D04-107-2Kg 2 Kg
EUR 224
DERMATOPHYTE TEST AGAR
D04-107-500g 500 g
EUR 97
SNYDER TEST AGAR
S19-119-10kg 10 kg
EUR 1049
SNYDER TEST AGAR
S19-119-2Kg 2 Kg
EUR 267
SNYDER TEST AGAR
S19-119-500g 500 g
EUR 109
SAM Test Strip
TS00201s-30 30 tests
EUR 416
Description: S-adenosylmethionine quantitative test strip for serum, plasma and whole blood
SAH Test Strip
TS00301s-30 30 tests
EUR 504
Description: S-adenosylhomocysteine quantitative test strip for serum, plasma and whole blood
HCY Test Strip
TS00401s-30 30 tests
EUR 553
Description: Serum homocysteine quantitative test strip
Human P311(Neuronal protein 3.1) ELISA Kit
EH4926 96T
EUR 567.6
  • Detection range: 0.156-10 ng/ml
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml
Human NNE(Non-Neuronal Enolase) ELISA Kit
EH0371 96T
EUR 524.1
  • Detection range: 0.313-20 ng/ml
  • Uniprot ID: P06733
  • Alias: NNE(Non-neural enolase)/ENO1/Alpha-enolase/MPB-1/MBP-1/Enolase 1/Plasminogen-binding protein/Phosphopyruvate hydratase/2-phospho-D-glycerate hydro-lyase/C-myc promoter-binding protein
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml
Human NPTXR(Neuronal pentraxin receptor) ELISA Kit
EH14986 96T
EUR 567.6
  • Detection range: 0.313-20 ng/ml
  • Uniprot ID: O95502
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml
Rat NNE(Non-Neuronal Enolase) ELISA Kit
ER1191 96T
EUR 524.1
  • Detection range: 0.313-20 ng/ml
  • Uniprot ID: P04764
  • Alias: NNE/ENO1/Alpha-enolase/MPB-1/MBP-1/Enolase 1/Plasminogen-binding protein/Phosphopyruvate hydratase/2-phospho-D-glycerate hydro-lyase/C-myc promoter-binding protein
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.188 ng/ml
2019-nCoV IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma)
GEN-402-25tests 25 tests
EUR 244
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.
ST Cell Serum-Free Medium-1
VCum-Lsx0001 1 L
EUR 740
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.
ST Cell Serum-Free Medium-2
VCum-Lsx0003 1 L
EUR 758
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.
PK15 Cell Serum-Free Medium (Powder)
VCum-Lsx0008-10L 10 L
EUR 2332
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.
PK15 Cell Serum-Free Medium (Powder)
VCum-Lsx0008-1L 1 L
EUR 278
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.
PK15 Cell Serum-Free Medium (Powder)
VCum-Lsx0008-5L 5 L
EUR 1191
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.
MDCK Cell Serum-Free Medium-1
VCum-Lsx0009 1 L
EUR 682
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells.
MDCK Cell Serum-Free Medium-2
VCum-Lsx0011 1 L
EUR 694
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production.
BHK Cell Serum-Free Medium-1
VCum-Lsx0017 1 L
EUR 647
Description: BHK cell serum-free medium has been specifically developed for suspension culture of BHK cells.
BHK Cell Serum-Free Medium-2
VCum-Lsx0019 1 L
EUR 717
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production.
Insect Cell Serum-Free Medium-1
VCum-Lsx0021 1 L
EUR 740
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).
Insect Cell Serum-Free Medium-2
VCum-Lsx0023 1 L
EUR 758
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).
CHO Cell Serum-Free Medium (Powder)
VCum-Lsx0026-10L 10 L
EUR 1747
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).
CHO Cell Serum-Free Medium (Powder)
VCum-Lsx0026-1L 1 L
EUR 220
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).
CHO Cell Serum-Free Medium (Powder)
VCum-Lsx0026-5L 5 L
EUR 898
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).
HEK Cell Serum-Free Medium (Powder)
VCum-Lsx0028 50 L
EUR 6411
Description: A serum-free medium that is ideal for suspension culture of HEK cell lines.
MDBK Cell Serum-Free Medium (Powder)
VCum-Lsx0030 50 L
EUR 8591
Description: Used in serum-free suspension MDBK cell line culture for bovine virus diarrhea or bovine rhinotracheitis vaccine production.
Vero Cell Serum-Free Medium (Powder)
VCum-Lsx0032-10L 10 L
EUR 2332
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.
Vero Cell Serum-Free Medium (Powder)
VCum-Lsx0032-1L 1 L
EUR 282
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.
Vero Cell Serum-Free Medium (Powder)
VCum-Lsx0032-5L 5 L
EUR 1208
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.
Human NEGR1(Neuronal growth regulator 1) ELISA Kit
EH14985 96T
EUR 567.6
  • Detection range: 0.156-10 ng/ml
  • Uniprot ID: Q7Z3B1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml
Human SEPT3(Neuronal-specific septin-3) ELISA Kit
EH1743 96T
EUR 567.6
  • Detection range: 0.312-20 ng/ml
  • Uniprot ID: Q9UH03
  • Alias: SEPT3/Neuronal-specific septin-3/SEP3
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml
Recombinant human Neuronal acetylcholine receptor subunit alpha-3
P1221 100ug Ask for price
  • Uniprot ID: P32297
  • Reconstitution: Metal affinity chromatography on Fn Super Capacity Column (Nickel)
Description: Recombinant protein for human Neuronal acetylcholine receptor subunit alpha-3
Recombinant human Neuronal acetylcholine receptor subunit beta-2
P2924 100ug Ask for price
  • Uniprot ID: P17787
  • Reconstitution: Metal affinity chromatography on Fn Super Capacity Column (Nickel)
Description: Recombinant protein for human Neuronal acetylcholine receptor subunit beta-2
Mounting Medium
4300 3 ml
EUR 180.5
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein.
VALI Medium
450 500 ml
EUR 265
BEGM Medium
451 500 ml
EUR 152
A Medium
DJ1018 100g
EUR 84.8
  • Product category: Culture Media/Medium
DC MEDIUM
D04-117-10kg 10 kg
EUR 1579
DC MEDIUM
D04-117-2kg 2kg
EUR 382
DC MEDIUM
D04-117-500g 500 g
EUR 140
EC MEDIUM
E05-100-10kg 10 kg
EUR 814
EC MEDIUM
E05-100-2Kg 2 Kg
EUR 216
EC MEDIUM
E05-100-500g 500 g
EUR 95
Advanced Medium
C0003-04 RT 500 mL Bottle
EUR 103
Medium 199
C0012-01 RT 500 mL Bottle
EUR 105
COLIFORM MEDIUM
C03-127-10kg 10 kg
EUR 1324
COLIFORM MEDIUM
C03-127-2kg 2kg
EUR 327
COLIFORM MEDIUM
C03-127-500g 500 g
EUR 125
Heller's Medium
CP014-010 10X1L
EUR 99
Heller's Medium
CP014-500 50L
EUR 126
Hoagland's Medium
CP015-010 10X1L
EUR 99
Hoagland's Medium
CP015-500 50L
EUR 126
HLP MEDIUM
H08-107-10kg 10 kg
EUR 2278
HLP MEDIUM
H08-107-2Kg 2 Kg
EUR 534
HLP MEDIUM
H08-107-500g 500 g
EUR 182
NeuroProgenitor Medium
NM42400 125 ml
EUR 304
M9 Medium
SD7024 250g
EUR 71.75
  • Product category: Culture Media/Medium
M9CA Medium
SD7025 250g
EUR 71.75
  • Product category: Culture Media/Medium
YM Medium
SD7031 250g
EUR 70.45
  • Product category: Culture Media/Medium
TYGPN Medium
SD7032 250g
EUR 70.01
  • Product category: Culture Media/Medium
M63 Medium
SD7033 250g
EUR 71.75
  • Product category: Culture Media/Medium
SIM MEDIUM
S19-110-10kg 10 kg
EUR 1021
SIM MEDIUM
S19-110-2kg 2kg
EUR 261
SIM MEDIUM
S19-110-500g 500 g
EUR 107
SOB MEDIUM
S19-124-10kg 10 kg
EUR 965
SOB MEDIUM
S19-124-2kg 2kg
EUR 249
SOB MEDIUM
S19-124-500g 500 g
EUR 104
50ml TC Tubes, Conical, 440 units/box
04-5540150 440 units/box
EUR 71
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