Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts

eaaci2015

Culture of Intestinal Epithelial Cell Monolayers and Their Use in Multiplex Macromolecular Permeability Assays for In Vitro Analysis of Tight Junction Size Selectivity

 

Tight junctions form a selectively permeable barrier that limits paracellular flux across epithelial-lined surfaces. Small molecules (less than ∼8 Å diameter) can traverse the junction via the size- and charge-selective, high-conductance pore pathway. In contrast, the low-conductance leak pathway accommodates larger macromolecules (up to ∼100 Å diameter) and is not charge-selective. Flux across the tight junction-independent, high-conductance, non-selective, unrestricted pathway occurs at sites of epithelial damage.

 

Cytokines can regulate each of these pathways, but commonly used measures of barrier function cannot discriminate between tight junction regulation and epithelial damage. This article describes methods for culturing intestinal epithelial cell monolayers and assessing the impact of cytokine treatment on leak and unrestricted pathway permeabilities. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Generation and culture of cell monolayers in Transwells Basic Protocol 2: Assessment of cytokine (IFNγ and TNF) treatment effects on barrier function Support Protocol: Immunofluorescent staining of monolayers Basic Protocol 3: Multiplex flux assay.

 

 

Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts

Introduction: Biomphalaria snails may display varying levels of susceptibility to Schistosoma mansoni infection. We have been developing an in vitro model to study the interaction between the snail and the parasite, using tissue-derived cell cultures from Biomphalaria.
Methods: The digestive gland- and kidney-derived cells from primary cultures of resistant (B. tenagophila Taim) and susceptible (B. tenagophila HM and B. glabrata BH) strains of Biomphalaria were exposed to S. mansoni sporocysts.
Results: S. mansoni sporocysts were surrounded and encapsulated exclusively by cells derived from the digestive gland (DG) of B. tenagophila Taim. The process was followed by a marked decrease in the number of free sporocysts in the culture medium. The morphological characteristics of DG-derived cells in culture have been described.
Conclusions: Cells derived from DG (but not SK) primary cultures of B. tenagophila Taim may participate in S. mansoni sporocyst control.
eaaci2015

eaaci2015

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myo-Inositol
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myo-Inositol
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myo-Inositol
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  • MW: 54.4 kDa
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Description: Recombinant Bacillus subtilis Inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase(iolG) expressed in E.coli
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Description: A competitive ELISA for quantitative measurement of Goat Inositol Triphosphate in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Inositol Triphosphate ELISA kit
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Description: A competitive ELISA for quantitative measurement of Goat Inositol Triphosphate in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Inositol Triphosphate ELISA kit
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Description: A competitive ELISA for quantitative measurement of Goat Inositol Triphosphate in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
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Description: A competitive ELISA for quantitative measurement of Rat Inositol Triphosphate in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Inositol Triphosphate ELISA kit
E02I0011-48 1 plate of 48 wells
EUR 520
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Description: A competitive ELISA for quantitative measurement of Rat Inositol Triphosphate in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Inositol Triphosphate ELISA kit
E02I0011-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rat Inositol Triphosphate in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Inositol Triphosphate ELISA kit
E03I0011-192T 192 tests
EUR 1270
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Description: A competitive ELISA for quantitative measurement of Mouse Inositol Triphosphate in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Inositol Triphosphate ELISA kit
E03I0011-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Mouse Inositol Triphosphate in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

 

 

Comparison of VEGF-A secretion from tumor cells under cellular stresses in conventional monolayer culture and microfluidic three-dimensional spheroid models

 

Vascular endothelial growth factor (VEGF) is a major cytokine in tumor biology affecting tumor survival, aggressiveness and pro-angiogenetic activities. In addition, cellular stresses often result in aggressive pro-angiogenetic behavior in tumors. For in vitro study, conventional monolayer cell culture has been broadly exploited; however, it often provides limited information due to its different microenvironment from that in vivo. Recently, three-dimensional (3D) cell spheroid culture provides in vivo-like microenvironments to study tumor biology and their survival mechanisms with better predictive power.
In this work, vascular endothelial growth factor of type A (VEGF-A) secretion from osteosarcoma (MG-63) cells cultured using monolayer and 3D spheroid models under two stress conditions: nutrient deficiency (reduced serum culture) and hypoxia-inducible factor (HIF) inhibition (HIF inhibitor, YC-1) are characterized and systematically compared.
In order to obtain ample sample size for consistent characterization of cellular responses from cancer spheroids under the stresses and compare the responses to those from the conventional monolayer model, a microfluidic spheroid formation and culture device is utilized in the experiments.
In the analysis, cell viability is estimated from captured images, and quantification of VEGF-A secreted from the cells is achieved using enzyme-linked immunosorbent assay (ELISA). The experimental results show that the viabilities decrease when the cells face higher stress levels in both monolayer and 3D spheroid culture models; however, the VEGF-A secretion profiles between the cell culture models are different.
The VEGF-A secretion decreases when the cells face higher stress conditions in the monolayer cell culture. In contrast, for the 3D spheroid culture, the VEGF-A concentration decreases for low stress levels but increases while the stress level is high. The VEGF-A regulation in the 3D models mimics in vivo cases of tumor survival and can provide insightful information to investigate tumor angiogenesis in vitro. The approach developed in this paper provides an efficient method to quantitatively and statistically study tumor growth kinetics and stress responses from highly uniform samples and it can also be applied to compare the underlying biomolecular mechanisms in monolayer and 3D spheroid culture models to elucidate the effects of microenvironments on cellular response in cancer research.

Protection of human induced pluripotent stem cells against shear stress in suspension culture by Bingham plastic fluid

 

Suspension culture is an important method used in the industrial preparation of pluripotent stem cells (PSCs), for regenerative therapy and drug screening. Generally, a suspension culture requires agitation to keep PSC aggregates suspended and to promote mass transfer, but agitation also causes cell damage. In this study, we investigated the use of a Bingham plastic fluid, supplemented with a polysaccharide-based polymer, to preserve PSCs from cell damage in suspension culture. Rheometric analysis showed that the culture medium gained yield stress and became a Bingham plastic fluid, after supplementation with the polymer FP003.

 

A growth/death analysis revealed that 2 days of aggregate formation and 2 days of suspension in the Bingham plastic medium improved cell growth and prevented cell death. After the initial aggregation step, whereas strong agitation (120 rpm) of a conventional culture medium resulted in massive cell death, in the Bingham plastic fluid we obtained the same growth as the normal culture with optimal agitation (90 rpm). This indicates that Bingham plastic fluid protected cells from shear stress in suspension culture and could be used to enhance their robustness when developing a large-scale. This article is protected by copyright. All rights reserved.

 

Metabolic profiling of CHO cell cultures at different working volumes and agitation speeds using spin tube reactors

 

Culture systems based on spin tube reactors have been consolidated in the development of manufacturing processes based on Chinese hamster ovary (CHO) cells. Despite their widespread use, there is little information about the consequences of varying operational setting parameters on the culture performance of recombinant CHO cell lines. Here we investigated the effect of varying working volumes and agitation speeds on cell growth, protein production and cell metabolism of two clonally-derived CHO cell lines (expressing an IgG1 and a ‘difficult-to-express’ fusion protein). Interestingly, low culture volumes increased recombinant protein production and decreased cell growth, while high culture volumes had the opposite effect. Altering agitation speeds exacerbated or moderated the differences observed due to culture volume changes.

 

Combining low agitation rates with high culture volumes suppressed growth and recombinant protein production in CHO cells. Meanwhile, high agitation rates narrowed the differences in culture performance between low and high working volumes. These differences were also reflected in cell metabolism, where low culture volumes enhanced oxidative metabolism (linked to a productive phenotype) and high culture volume generated a metabolic profile that was predominately glycolytic (linked to a proliferative phenotype). Our findings indicate that the culture volume influence on metabolism modulates the balance between cell growth and protein production, a key feature that may be useful to adjust CHO cells towards a more productive phenotype.

 

1-(Fmoc-amino)-cyclopentanecarboxylic acid

B-3570.0005 5.0g
EUR 998
Description: Sum Formula: C21H21NO4; CAS# [117322-30-2]

Toolbox Fmoc-Amino Acid Derivatives

B-3840.0001 100.0g
EUR 624

3-Amino-5-bromobenzoic acid

GM0393-25G 25 g
EUR 190

2-Amino-5-chlorobenzoic acid

GM8338-10G 10 g
EUR 78

2-Amino-5-chlorobenzoic acid

GM8338-50G 50 g
EUR 134

β-Amino Acid Imagabalin Hydrochloride

HY-U00250 20mg
EUR 13439

5-Amino-4-oxopentanoic acid

HY-W000450 100mg
EUR 119

3-Amino-4-hydroxybenzoic acid

HY-W010224 100mg
EUR 108

3-Amino-4-methylpentanoic acid

HY-W012708 100mg
EUR 119

3-Amino-2-methylpropanoic acid

HY-W012974 100mg
EUR 108

D-Amino Acid Oxidase (Recombinant)

20-abx073765
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

YNB W / O Amino acid

abx082010-100g 100 g
EUR 230
  • Shipped within 5-10 working days.

6-Amino-2-picolinic acid

20-abx183769
  • EUR 425.00
  • EUR 244.00
  • 100 g
  • 25 g
  • Shipped within 1-2 weeks.

cis-3-Amino-cyclobutanecarboxylic acid

abx183945-1g 1 g
EUR 1219
  • Shipped within 1-2 weeks.

3-Amino-4-methylbenzoic acid

20-abx184574
  • EUR 258.00
  • EUR 495.00
  • 100 g
  • 500 g
  • Shipped within 1-2 weeks.

2-Amino-4-pyridinecarboxylic acid

20-abx185019
  • EUR 370.00
  • EUR 203.00
  • EUR 230.00
  • 100 g
  • 10 g
  • 25 g
  • Shipped within 1-2 weeks.

4-Amino-3-methoxybenzoic acid

20-abx185187
  • EUR 565.00
  • EUR 300.00
  • EUR 217.00
  • 100 g
  • 25 g
  • 5 g
  • Shipped within 1-2 weeks.

2-Amino-4-Hydroxybenzoic Acid

20-abx188200
  • EUR 1748.00
  • EUR 509.00
  • EUR 1191.00
  • 10 g
  • 1 g
  • 5 g
  • Shipped within 1-2 weeks.

2-Amino-5-Fluorobenzoic Acid

abx188201-100g 100 g
EUR 342
  • Shipped within 1-2 weeks.

3-Amino-2-Methylbenzoic Acid

abx188349-1000g 1000 g
EUR 773
  • Shipped within 1-2 weeks.

2-Amino-5-pyrazinecarboxylic acid

20-abx180281
  • EUR 996.00
  • EUR 286.00
  • EUR 634.00
  • 10 g
  • 1 g
  • 5 g
  • Shipped within 1-2 weeks.

4-(Boc-Amino)Benzeneboronic Acid

20-abx180465
  • EUR 1135.00
  • EUR 300.00
  • EUR 467.00
  • 100 g
  • 10 g
  • 25 g
  • Shipped within 1-2 weeks.

2-Amino-3,5-dichlorobenzoic acid

20-abx181010
  • EUR 467.00
  • EUR 300.00
  • EUR 217.00
  • 100 g
  • 25 g
  • 5 g
  • Shipped within 1-2 weeks.

(PEO)4-amino-propionic acid

20-abx181441
  • EUR 606.00
  • EUR 356.00
  • EUR 1344.00
  • 1 g
  • 250 mg
  • 5 g
  • Shipped within 1-2 weeks.

2-Amino-5-bromonicotinic acid

20-abx181694
  • EUR 857.00
  • EUR 300.00
  • 100 g
  • 10 g
  • Shipped within 1-2 weeks.

2-Amino-5-Pyrimidineboronic Acid

20-abx181700
  • EUR 1316.00
  • EUR 509.00
  • EUR 244.00
  • 100 g
  • 25 g
  • 5 g
  • Shipped within 1-2 weeks.

2-Amino-2-phenylacetic acid

20-abx183045
  • EUR 217.00
  • EUR 314.00
  • 100 g
  • 500 g
  • Shipped within 1-2 weeks.

2-Amino-4,5-dimethoxybenzoic acid

20-abx183054
  • EUR 258.00
  • EUR 203.00
  • 100 g
  • 25 g
  • Shipped within 1-2 weeks.

2-Amino-4-methoxybenzoic acid

abx183059-100g 100 g
EUR 954
  • Shipped within 1-2 weeks.

2-Amino-6-methoxybenzoic acid

20-abx183075
  • EUR 690.00
  • EUR 300.00
  • 100 g
  • 25 g
  • Shipped within 1-2 weeks.

2-Amino-6-nitrobenzoic acid

20-abx183077
  • EUR 286.00
  • EUR 1233.00
  • EUR 523.00
  • 1 g
  • 25 g
  • 5 g
  • Shipped within 1-2 weeks.

3-Amino-5-bromopicolinic acid

20-abx181890
  • EUR 940.00
  • EUR 634.00
  • EUR 2513.00
  • 1 g
  • 500 mg
  • 5 g
  • Shipped within 1-2 weeks.

4-Amino-3-hydroxybenzoic acid

20-abx182035
  • EUR 258.00
  • EUR 189.00
  • 25 g
  • 5 g
  • Shipped within 1-2 weeks.

11-(Boc-amino)-undecanoic acid

A-3310.0005 5.0g
EUR 151
Description: Sum Formula: C16H31NO4; CAS# [10436-25-6]

11-(Boc-amino)-undecanoic acid

A-3310.0025 25.0g
EUR 515
Description: Sum Formula: C16H31NO4; CAS# [10436-25-6]

1-(Z-amino)Cyclohexanecarboxylic acid

5-02277 5g Ask for price

1-(Z-amino)Cyclohexanecarboxylic acid

5-02278 25g Ask for price

L Amino Acid Quantitation Kit

55R-1479 100 assays
EUR 654
Description: L Amino Acid Quantitation Kit for use in the research laboratory

anti-D Amino Acid Oxidase

YF-PA23561 50 ul
EUR 334
Description: Mouse polyclonal to D Amino Acid Oxidase

Resveratrol, synthetic

GP5884-100MG 100 mg
EUR 59

Resveratrol, synthetic

GP5884-1G 1 g
EUR 158

Resveratrol, synthetic

GP5884-250MG 250 mg
EUR 87

Lactacystin (Synthetic)

A2583-.1 100 µg
EUR 150
Description: Lactacystin is a specific and an irreversible inhibitor of proteasome with IC50 value of 4.8 ?M [1].Lactacystin binds to the catalytic subunits of the 20 S proteasome and inhibits all the three peptidase activities of the proteasome, chymotrypsin-like, trypsin-like and caspase-like.

Lactacystin (Synthetic)

A2583-.5 500 µg
EUR 473
Description: Lactacystin is a specific and an irreversible inhibitor of proteasome with IC50 value of 4.8 ?M [1].Lactacystin binds to the catalytic subunits of the 20 S proteasome and inhibits all the three peptidase activities of the proteasome, chymotrypsin-like, trypsin-like and caspase-like.

Lactacystin (Synthetic)

A2583-1 1 mg
EUR 790
Description: Lactacystin is a specific and an irreversible inhibitor of proteasome with IC50 value of 4.8 ?M [1].Lactacystin binds to the catalytic subunits of the 20 S proteasome and inhibits all the three peptidase activities of the proteasome, chymotrypsin-like, trypsin-like and caspase-like.

Lactacystin (Synthetic)

1709-200
EUR 316

(S)-(-)-2-Amino-4-pentenoic acid

GM0237-1G 1 g
EUR 90

(S)-(-)-2-Amino-4-pentenoic acid

GM0237-5G 5 g
EUR 237

Human Amino Acid Metabolism Primer Library

HAMM-I 1 set
EUR 645

4-Amino-piperidine-4-carboxylic acid

F-3605.0001 1.0g
EUR 345
Description: Sum Formula: C6H12N2O2; CAS# [40951-39-1] net

4-Amino-piperidine-4-carboxylic acid

F-3605.0005 5.0g
EUR 1301
Description: Sum Formula: C6H12N2O2; CAS# [40951-39-1] net

4-Amino-piperidine-4-carboxylic acid

F-3605.0025 25.0g
EUR 5108
Description: Sum Formula: C6H12N2O2; CAS# [40951-39-1] net

4-Amino-3-phenylbutyric acid HCl

20-abx183511
  • EUR 230.00
  • EUR 732.00
  • EUR 356.00
  • 1 g
  • 25 g
  • 5 g
  • Shipped within 1-2 weeks.

Fmoc-21-Amino-4,7,10,13,16,19-Hexaoxaheneicosanoic Acid

20-abx184074
  • EUR 871.00
  • EUR 606.00
  • 1 g
  • 500 mg
  • Shipped within 1-2 weeks.

Benzoic acid, o-amino-, ethyl ester

20-abx184649
  • EUR 286.00
  • EUR 217.00
  • EUR 578.00
  • 100 g
  • 25 g
  • 500 g
  • Shipped within 1-2 weeks.

Boc-9-AMino-4,7-Dioxanonanoic acid

20-abx186634
  • EUR 370.00
  • EUR 495.00
  • 0.5 g
  • 1 g
  • Shipped within 1-2 weeks.

Boc-15-Amino-4,7,10,13-Tetraoxapentadecanoic Acid

20-abx186635
  • EUR 439.00
  • EUR 578.00
  • 0.5 g
  • 1 g
  • Shipped within 1-2 weeks.

Fmoc-12-Amino-4,7,10-Trioxadodecanoic Acid

abx186636-1g 1 g
EUR 370
  • Shipped within 1-2 weeks.

2-((2-Aminoethyl)Amino)Acetic Acid

20-abx188143
  • EUR 1261.00
  • EUR 634.00
  • 25 g
  • 5 g
  • Shipped within 1-2 weeks.

2-Amino-Isonicotinic Acid Ethyl Ester

20-abx188205
  • EUR 718.00
  • EUR 356.00
  • 100 g
  • 25 g
  • Shipped within 1-2 weeks.

2-Hydroxy-4-Amino Butanoic Acid

abx188257-1kg 1 kg
EUR 746
  • Shipped within 1-2 weeks.

D-Amino-Acid Oxidase (DAO) Antibody

abx431198-200ul 200 ul
EUR 286
  • Shipped within 1-3 working days.

D-Amino-Acid Oxidase (DAO) Antibody

abx432587-200ul 200 ul
EUR 384
  • Shipped within 1-3 working days.

D-Amino-Acid Oxidase (DAO) Antibody

abx432588-200ul 200 ul
EUR 384
  • Shipped within 1-3 working days.

5-Amino-2-chloro-isonicotinic acid

20-abx182161
  • EUR 1066.00
  • EUR 843.00
  • 25 g
  • 5 g
  • Shipped within 1-2 weeks.

Recombinant Human D-Amino Acid Oxidase

7-02629 2µg Ask for price

Recombinant Human D-Amino Acid Oxidase

7-02630 10µg Ask for price

Recombinant Human D-Amino Acid Oxidase

7-02631 1mg Ask for price

Fmoc-2-amino-5-Methoxybenzoic acid

5-04549 1g Ask for price

Fmoc-2-amino-5-Methoxybenzoic acid

5-04550 5g Ask for price

Human D Amino Acid Oxidase Antibody

32963-05111 150 ug
EUR 261

Branched Chain Amino Acid Assay Kit

55R-1440 100 assays
EUR 740
Description: Assay Kit for detection of Branched Chain Amino Acids in the research laboratory

Fructosyl-amino acid oxidase from Microorganism

DIA-274 1ku
EUR 270

Human D-amino-acid oxidase (DAO)

1-CSB-YP006494HU
  • EUR 430.00
  • EUR 234.00
  • EUR 1508.00
  • EUR 642.00
  • EUR 1009.00
  • EUR 291.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 41.5 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human D-amino-acid oxidase(DAO) expressed in Yeast

Human D-amino-acid oxidase (DAO)

1-CSB-EP006494HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 55.5 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human D-amino-acid oxidase(DAO) expressed in E.coli

Rat D-amino-acid oxidase (Dao)

1-CSB-EP006494RA
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 45.8 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Rat D-amino-acid oxidase(Dao) expressed in E.coli

Native Porcine D-Amino Acid Oxidase

NATE-0180 400u
EUR 270

D-amino acid oxidase, human recombinant

P1052-10
EUR 164

D-amino acid oxidase, human recombinant

P1052-50
EUR 588

Recombinant rat D-amino-acid oxidase

P1766 100ug Ask for price
  • Uniprot ID: O35078
  • Reconstitution: Metal affinity chromatography on Fn Super Capacity Column (Nickel)
Description: Recombinant protein for rat D-amino-acid oxidase

L-Amino Acid Assay Kit (Colorimetric)

MET-5054 100 assays
EUR 409

L-Amino Acid Assay Kit (Fluorometric)

MET-5055 100 assays
EUR 409

Branched Chain Amino Acid Assay Kit

MET-5056 192 assays
EUR 450

Anti-D-amino-acid oxidase antibody

STJ71455 100 µg
EUR 359

Anti-D Amino Acid Oxidase (2F5)

YF-MA12633 100 ug
EUR 363
Description: Mouse monoclonal to D Amino Acid Oxidase

Synthetic pyrethroid insecticide

AG123 1 mg
EUR 523

Synthetic pyrethroid insecticides

AT123 1mg
EUR 1368

Atpenin A5 (Synthetic)

2210-1000
EUR 457

Atpenin A5 (Synthetic)

2210-250
EUR 191

Synthetic Triacylated Lipopeptide

VAdv-Ly0036 1 mg
EUR 1628
Description: Synthetic triacylated lipopeptide, a synthetic lipopeptide and a TLR2/1 agonist vaccine adjuvant.

Synthetic Neurotensin (NT)

4-SPB203Hu01
  • EUR 368.80
  • EUR 202.00
  • EUR 1108.00
  • EUR 436.00
  • EUR 772.00
  • EUR 310.00
  • EUR 2620.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P30990
  • Buffer composition: PBS, pH 7.4.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 1561.9Da
  • Isoelectric Point: 9.7
Description: Recombinant Human Neurotensin expressed in: E.coli

Synthetic Phytohemagglutinin (PHA)

4-SPB359Ge01
  • EUR 368.80
  • EUR 202.00
  • EUR 1108.00
  • EUR 436.00
  • EUR 772.00
  • EUR 310.00
  • EUR 2620.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Inquire
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): Inquire
  • Isoelectric Point: Inquire
Description: Recombinant Pan-species Phytohemagglutinin expressed in: E.coli

Polyclonal Excitatory Amino Acid Transporter 3 Antibody

APR11879G 0.05ml
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Excitatory Amino Acid Transporter 3 . This antibody is tested and proven to work in the following applications:

4-Amino-3-hydroxy-1-naphthalenesulfonic acid

GW5931-100G 100 g
EUR 93

4-Amino-3-hydroxy-1-naphthalenesulfonic acid

GW5931-25G 25 g
EUR 51

3-Amino-1-propanesulfonic acid sodium salt

GL4935-1G 1 g
EUR 94

2(S)-Amino-4-azido-butanoic acid

GM3282-100MG 100 mg
EUR 378

Porcine Branched- chain- amino- acid aminotransferase, mitochond

ELI-34731p 96 Tests
EUR 928

Branched Chain Amino-Acid Transaminase 1 (Recombinant)

20-abx073338
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Branched Chain Amino-Acid Transaminase 2 (Recombinant)

20-abx073802
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

Cationic Amino Acid Transporter 4 (SLC7A4) Antibody

20-abx115716
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

Excitatory Amino Acid Transporter 3 (EAAT3) Antibody

20-abx128437
  • EUR 425.00
  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

D-Amino Acid Oxidase Activator (DAOA) Antibody

20-abx109711
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Cationic Amino Acid Transporter 1 (CAT1) Antibody

20-abx141511
  • EUR 370.00
  • EUR 606.00
  • EUR 300.00
  • 100 ul
  • 200 ul
  • 50 ul
  • Shipped within 5-10 working days.

Cationic Amino Acid Transporter 4 (SLC7A4) Antibody

abx034704-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Cationic Amino Acid Transporter 4 (SLC7A4) Antibody

abx034704-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

Cationic Amino Acid Transporter 4 (SLC7A4) Antibody

20-abx014327
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.

4-Amino-2-fluorophenylboronic acid pinacol ester

20-abx183508
  • EUR 1038.00
  • EUR 328.00
  • EUR 634.00
  • 10 g
  • 1 g
  • 5 g
  • Shipped within 1-2 weeks.

Boc-(R)-3-Amino-4-phenylbutyric acid

20-abx183891
  • EUR 662.00
  • EUR 1636.00
  • 1 g
  • 5 g
  • Shipped within 1-2 weeks.

4-Amino-5-hydroxy-2,7-naphthalenedisulfonic acid

abx185191-10g 10 g
EUR 203
  • Shipped within 1-2 weeks.

(S)-2-(Z-Amino)-4-Phenylbutyric Acid

20-abx186099
  • EUR 230.00
  • EUR 565.00
  • EUR 300.00
  • 1 g
  • 25 g
  • 5 g
  • Shipped within 1-2 weeks.

2-amino-4-methylpyridine-3-carboxylic acid

20-abx186199
  • EUR 871.00
  • EUR 2291.00
  • 1 g
  • 5 g
  • Shipped within 1-2 weeks.

2-Amino-5-Naphthol-7-Sulfonic Acid

abx188202-500g 500 g
EUR 286
  • Shipped within 1-2 weeks.

3-((Tert-Butoxycarbonyl)Amino)-2,2-Dimethylpropanoic Acid

abx188284-100g 100 g
EUR 3585
  • Shipped within 1-2 weeks.

4-Amino-3-Fluorophenylboronic Acid Pinacol Ester

abx188432-50g 50 g
EUR 1664
  • Shipped within 1-2 weeks.

D-Amino Acid Oxidase / DAMOX (DAO) Antibody

20-abx172043
  • EUR 857.00
  • EUR 439.00
  • 1 mg
  • 200 ug
  • Please enquire.

D-amino Acid Oxidase Activator (DAOA) Antibody

20-abx172044
  • EUR 857.00
  • EUR 439.00
  • 1 mg
  • 200 ug
  • Please enquire.

Excitatory Amino Acid Transporter 1 (EAAT1) Antibody

20-abx172302
  • EUR 857.00
  • EUR 439.00
  • 1 mg
  • 200 ug
  • Please enquire.

Excitatory Amino Acid Transporter 2 (EAAT2) Antibody

20-abx172303
  • EUR 857.00
  • EUR 439.00
  • 1 mg
  • 200 ug
  • Please enquire.

Excitatory Amino Acid Transporter 2 (EAAT2) Antibody

20-abx172304
  • EUR 913.00
  • EUR 467.00
  • 1 mg
  • 200 ug
  • Please enquire.

Excitatory Amino Acid Transporter 3 (EAAT3) Antibody

20-abx172305
  • EUR 857.00
  • EUR 439.00
  • 1 mg
  • 200 ug
  • Please enquire.

Excitatory Amino Acid Transporter 4 (EAAT4) Antibody

20-abx172306
  • EUR 857.00
  • EUR 439.00
  • 1 mg
  • 200 ug
  • Please enquire.

Excitatory Amino Acid Transporter 5 (EAAT5) Antibody

20-abx172307
  • EUR 857.00
  • EUR 439.00
  • 1 mg
  • 200 ug
  • Please enquire.

Cationic Amino Acid Transporter 1 (CAT1) Antibody

20-abx175745
  • EUR 1233.00
  • EUR 592.00
  • 1 mg
  • 200 ug
  • Please enquire.

Cationic Amino Acid Transporter 1 (CAT1) Antibody

20-abx175746
  • EUR 1233.00
  • EUR 592.00
  • 1 mg
  • 200 ug
  • Please enquire.

Cationic Amino Acid Transporter 2 (CAT2) Antibody

20-abx175747
  • EUR 1302.00
  • EUR 620.00
  • 1 mg
  • 200 ug
  • Please enquire.

D-Amino Acid Oxidase / DAMOX (DAO) Antibody

20-abx176101
  • EUR 425.00
  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-12 working days.

D-amino Acid Oxidase Activator (DAOA) Antibody

20-abx176102
  • EUR 1205.00
  • EUR 578.00
  • 1 mg
  • 200 ug
  • Please enquire.

Excitatory Amino Acid Transporter 1 (EAAT1) Antibody

20-abx176336
  • EUR 1205.00
  • EUR 578.00
  • 1 mg
  • 200 ug
  • Please enquire.

Excitatory Amino Acid Transporter 1 (EAAT1) Antibody

20-abx176337
  • EUR 1233.00
  • EUR 592.00
  • 1 mg
  • 200 ug
  • Please enquire.

Excitatory Amino Acid Transporter 1 (EAAT1) Antibody

20-abx176338
  • EUR 1233.00
  • EUR 592.00
  • 1 mg
  • 200 ug
  • Please enquire.

Excitatory Amino Acid Transporter 2 (EAAT2) Antibody

20-abx176339
  • EUR 425.00
  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-12 working days.

Excitatory Amino Acid Transporter 2 (EAAT2) Antibody

20-abx176340
  • EUR 439.00
  • EUR 133.00
  • EUR 1233.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-12 working days.

Excitatory Amino Acid Transporter 2 (EAAT2) Antibody

20-abx176341
  • EUR 1233.00
  • EUR 592.00
  • 1 mg
  • 200 ug
  • Please enquire.

Excitatory Amino Acid Transporter 2 (EAAT2) Antibody

20-abx176342
  • EUR 1302.00
  • EUR 620.00
  • 1 mg
  • 200 ug
  • Please enquire.

Excitatory Amino Acid Transporter 5 (EAAT5) Antibody

20-abx176343
  • EUR 1205.00
  • EUR 578.00
  • 1 mg
  • 200 ug
  • Please enquire.

Cationic Amino Acid Transporter 4 (SLC7A4) Antibody

20-abx323283
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

Cationic Amino Acid Transporter 4 (SLC7A4) Antibody

abx237974-100ug 100 ug
EUR 551
  • Shipped within 5-12 working days.

D-Amino Acid Oxidase/ DAMOX (DAO) Antibody

20-abx301170
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Aromatic-L-Amino-Acid Decarboxylase (DDC) Antibody

20-abx302246
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

2-Amino-3-bromo-5-chlorobenzoic acid

20-abx180268
  • EUR 217.00
  • EUR 328.00
  • 1 g
  • 5 g
  • Shipped within 1-2 weeks.

Cis-2-amino-1-methyl-cyclopentanecarboxylic acid

20-abx180705
  • EUR 2541.00
  • EUR 1024.00
  • 1 g
  • 250 mg
  • Shipped within 1-2 weeks.

(2-Amino-4-Methoxycarbonylphenyl)Boronic Acid Hydrochloride

20-abx181397
  • EUR 1219.00
  • EUR 300.00
  • EUR 787.00
  • 10 g
  • 1 g
  • 5 g
  • Shipped within 1-2 weeks.

2-Amino-2-(4-Methoxyphenyl)Acetic Acid

20-abx181673
  • EUR 384.00
  • EUR 815.00
  • 1 g
  • 5 g
  • Shipped within 1-2 weeks.

D-Amino Acid Oxidase/ DAMOX (DAO) Antibody

abx232235-100ug 100 ug
EUR 481
  • Shipped within 5-12 working days.

D-Amino Acid Oxidase Activator (DAOA) Antibody

abx232236-100ug 100 ug
EUR 481
  • Shipped within 5-12 working days.

(S)-2-((Methoxycarbonyl)amino)-3-methylbutanoic acid

20-abx182776
  • EUR 411.00
  • EUR 189.00
  • EUR 286.00
  • EUR 217.00
  • 100 g
  • 1 g
  • 25 g
  • 5 g
  • Shipped within 1-2 weeks.

2-Amino-6-Methyl-3-Pyridinecarboxylic Acid

20-abx183076
  • EUR 1817.00
  • EUR 523.00
  • EUR 1107.00
  • 10 g
  • 1 g
  • 5 g
  • Shipped within 1-2 weeks.

3-Amino-Pyridine-2-Carboxylic Acid Amide

20-abx183338
  • EUR 746.00
  • EUR 272.00
  • EUR 509.00
  • 10 g
  • 1 g
  • 5 g
  • Shipped within 1-2 weeks.

Cationic Amino Acid Transporter 2 (CAT2) Antibody

20-abx171644
  • EUR 913.00
  • EUR 467.00
  • 1 mg
  • 200 ug
  • Please enquire.

3-Amino-2-methylpyridine-5-carboxylic acid

20-abx181888
  • EUR 2360.00
  • EUR 1330.00
  • 1 g
  • 250 mg
  • Shipped within 1-2 weeks.

4-amino-6-chloropyridine-3-carboxylic acid

20-abx182038
  • EUR 732.00
  • EUR 398.00
  • EUR 1873.00
  • 1 g
  • 250 mg
  • 5 g
  • Shipped within 1-2 weeks.

4-Pyrimidinecarboxylic Acid, 6-Amino-, Methyl Ester

20-abx182141
  • EUR 3376.00
  • EUR 843.00
  • EUR 2193.00
  • 10 g
  • 1 g
  • 5 g
  • Shipped within 1-2 weeks.

D-amino acid oxidase activator Polyclonal Antibody

42143-100ul 100ul
EUR 333

L-Amino Acid Quantitation Colorimetric/Fluorometric Kit

K2141-100 100 assays
EUR 502

Total D-Amino Acid Assay Kit (Fluorometric)

K445-100
EUR 533

L-Amino Acid Quantitation Colorimetric/Fluorometric Kit

K639-100
EUR 479

Mouse Excitatory amino acid transporter 2 (Slc1a2)

1-CSB-YP021433MO
  • EUR 504.00
  • EUR 265.00
  • EUR 1832.00
  • EUR 763.00
  • EUR 1216.00
  • EUR 334.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 37.6 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Mouse Excitatory amino acid transporter 2(Slc1a2),partial expressed in Yeast

Human D-amino acid oxidase activator (DAOA)

1-CSB-EP006495HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 45.1 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human D-amino acid oxidase activator(DAOA) expressed in E.coli

Human xcitatory amino acid transporter 3 (SLC1A1)

1-CSB-EP021432HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 37.5 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human xcitatory amino acid transporter 3(SLC1A1),partial expressed in E.coli

Mouse Excitatory amino acid transporter 2 (Slc1a2)

1-CSB-EP021433MO
  • EUR 505.00
  • EUR 265.00
  • EUR 1827.00
  • EUR 766.00
  • EUR 1218.00
  • EUR 335.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 37.6 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Mouse Excitatory amino acid transporter 2(Slc1a2),partial expressed in E.coli

Human Excitatory amino acid transporter 4 (SLC1A6)

1-CSB-EP021437HU
  • EUR 437.00
  • EUR 238.00
  • EUR 1544.00
  • EUR 653.00
  • EUR 1029.00
  • EUR 296.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 40.4 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Excitatory amino acid transporter 4(SLC1A6),partial expressed in E.coli