Culture of Intestinal Epithelial Cell Monolayers and Their Use in Multiplex Macromolecular Permeability Assays for In Vitro Analysis of Tight Junction Size Selectivity
Tight junctions form a selectively permeable barrier that limits paracellular flux across epithelial-lined surfaces. Small molecules (less than ∼8 Å diameter) can traverse the junction via the size- and charge-selective, high-conductance pore pathway. In contrast, the low-conductance leak pathway accommodates larger macromolecules (up to ∼100 Å diameter) and is not charge-selective. Flux across the tight junction-independent, high-conductance, non-selective, unrestricted pathway occurs at sites of epithelial damage.
Cytokines can regulate each of these pathways, but commonly used measures of barrier function cannot discriminate between tight junction regulation and epithelial damage. This article describes methods for culturing intestinal epithelial cell monolayers and assessing the impact of cytokine treatment on leak and unrestricted pathway permeabilities. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Generation and culture of cell monolayers in Transwells Basic Protocol 2: Assessment of cytokine (IFNγ and TNF) treatment effects on barrier function Support Protocol: Immunofluorescent staining of monolayers Basic Protocol 3: Multiplex flux assay.
Paraffin Wax Dispenser |
Paraffin wax, granular (56 - 60) |
Paraffin wax, granular (56 - 60) |
MagSi-WAX |
MagSi-WAX |
MagSi-WAX |
Wax Paper For Se235 Pkg100 |
neo WAX 30mg Plate SPE Columns |
neo WAX 100mg/3mL SPE Columns |
neo WAX 200mg/6mL SPE Columns |
Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts

eaaci2015
myo-Inositol |
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GC4902-100G | Glentham Life Sciences | 100 g | EUR 62.4 |
myo-Inositol |
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GC4902-1KG | Glentham Life Sciences | 1 kg | EUR 180 |
myo-Inositol |
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GC4902-250G | Glentham Life Sciences | 250 g | EUR 84 |
myo-Inositol |
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GC4902-25G | Glentham Life Sciences | 25 g | EUR 48 |
myo-Inositol |
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GC4902-500G | Glentham Life Sciences | 500 g | EUR 117.6 |
i-Inositol |
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HY-B1411 | MedChemExpress | 5g | EUR 142.8 |
Myo-inositol |
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TBZ2449 | ChemNorm | 20mg | Ask for price |
Screen Quest™ Rhod-4 No Wash Calcium Assay Kit *Medium Removal* |
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36330 | AAT Bioquest | 1 Plate | EUR 158.4 |
Screen Quest™ Rhod-4 No Wash Calcium Assay Kit *Medium Removal* |
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36331 | AAT Bioquest | 10 Plates | EUR 471.6 |
Screen Quest™ Rhod-4 No Wash Calcium Assay Kit *Medium Removal* |
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36332 | AAT Bioquest | 100 Plates | EUR 3139.2 |
D-myo-Inositol |
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IB0536 | Bio Basic | 100g | EUR 80.88 |
1D-chiro-Inositol |
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GC3561-100MG | Glentham Life Sciences | 100 mg | EUR 122.4 |
1D-chiro-Inositol |
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GC3561-1G | Glentham Life Sciences | 1 g | EUR 322.8 |
1L-chiro-Inositol |
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GC7626-100MG | Glentham Life Sciences | 100 mg | EUR 122.4 |
1L-chiro-Inositol |
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GC7626-1G | Glentham Life Sciences | 1 g | EUR 322.8 |
D-chiro-Inositol |
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HY-N3021 | MedChemExpress | 10mg | EUR 129.6 |
1D-chiro-Inositol |
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TPC3089 | ChemNorm | 20mg | Ask for price |
A Medium |
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DJ1018 | Bio Basic | 100g | EUR 101.76 |
DC MEDIUM |
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D04-117-10kg | Alphabiosciences | 10 kg | EUR 1894.8 |
DC MEDIUM |
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D04-117-2kg | Alphabiosciences | 2kg | EUR 458.4 |
DC MEDIUM |
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D04-117-500g | Alphabiosciences | 500 g | EUR 168 |
Advanced Medium |
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C0003-04 | Addexbio | RT 500 mL Bottle | EUR 123.6 |
Medium 199 |
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C0012-01 | Addexbio | RT 500 mL Bottle | EUR 126 |
COLIFORM MEDIUM |
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C03-127-10kg | Alphabiosciences | 10 kg | EUR 1588.8 |
COLIFORM MEDIUM |
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C03-127-2kg | Alphabiosciences | 2kg | EUR 392.4 |
COLIFORM MEDIUM |
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C03-127-500g | Alphabiosciences | 500 g | EUR 150 |
Heller's Medium |
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CP014-010 | GenDepot | 10X1L | EUR 118.8 |
Heller's Medium |
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CP014-500 | GenDepot | 50L | EUR 151.2 |
Hoagland's Medium |
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CP015-010 | GenDepot | 10X1L | EUR 118.8 |
Hoagland's Medium |
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CP015-500 | GenDepot | 50L | EUR 151.2 |
EC MEDIUM |
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E05-100-10kg | Alphabiosciences | 10 kg | EUR 976.8 |
EC MEDIUM |
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E05-100-2Kg | Alphabiosciences | 2 Kg | EUR 259.2 |
EC MEDIUM |
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E05-100-500g | Alphabiosciences | 500 g | EUR 114 |
Mounting Medium |
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4300 | Virostat | 3 ml | EUR 216.6 |
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein. |
NeuroProgenitor Medium |
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NM42400 | Neuromics | 125 ml | EUR 364.8 |
HLP MEDIUM |
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H08-107-10kg | Alphabiosciences | 10 kg | EUR 2733.6 |
HLP MEDIUM |
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H08-107-2Kg | Alphabiosciences | 2 Kg | EUR 640.8 |
HLP MEDIUM |
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H08-107-500g | Alphabiosciences | 500 g | EUR 218.4 |
SIM MEDIUM |
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S19-110-10kg | Alphabiosciences | 10 kg | EUR 1225.2 |
SIM MEDIUM |
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S19-110-2kg | Alphabiosciences | 2kg | EUR 313.2 |
SIM MEDIUM |
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S19-110-500g | Alphabiosciences | 500 g | EUR 128.4 |
SOB MEDIUM |
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S19-124-10kg | Alphabiosciences | 10 kg | EUR 1158 |
SOB MEDIUM |
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S19-124-2kg | Alphabiosciences | 2kg | EUR 298.8 |
SOB MEDIUM |
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S19-124-500g | Alphabiosciences | 500 g | EUR 124.8 |
M9 Medium |
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SD7024 | Bio Basic | 250g | EUR 86.1 |
M9CA Medium |
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SD7025 | Bio Basic | 250g | EUR 86.1 |
YM Medium |
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SD7031 | Bio Basic | 250g | EUR 84.54 |
TYGPN Medium |
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SD7032 | Bio Basic | 250g | EUR 84.01 |
M63 Medium |
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SD7033 | Bio Basic | 250g | EUR 86.1 |
TCBS Medium |
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MED1188 | Scientific Laboratory Supplies | PK10 | EUR 7.52 |
DNase Medium |
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MED1196 | Scientific Laboratory Supplies | PK10 | EUR 7.98 |
OF Medium |
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MED1664 | Scientific Laboratory Supplies | 500G | EUR 68.4 |
Inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase Polyclonal Antibody |
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42631-100ul | SAB | 100ul | EUR 399.6 |
Inositol 2-Dehydrogenase/D-chiro-Inositol 3-Dehydrogenase (IOLG) Antibody |
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20-abx110510 | Abbexa |
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Inositol Monophosphatase 1 (Recombinant) |
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20-abx073158 | Abbexa |
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Inositol Monophosphatase 2 (Recombinant) |
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20-abx073694 | Abbexa |
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Myo-Inositol Oxygenase (Recombinant) |
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20-abx073852 | Abbexa |
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L-alpha-Phosphatidyl inositol |
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GE1414-500MG | Glentham Life Sciences | 500 mg | EUR 151.2 |
Insect Cell Medium: TNM-FH Insect Culture Medium |
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ABP-MED-10001 | Allele Biotech | 1 liter | Ask for price |
Insect Cell Medium: Serum-Free Insect Culture Medium |
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ABP-MED-10002 | Allele Biotech | 1 liter | Ask for price |
Insect Cell Medium: Grace?s Insect Medium (Unsupplemented) |
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ABP-MED-10004 | Allele Biotech | 1 liter | Ask for price |
MCDB 131 Medium |
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CM034-050 | GenDepot | 500ml | EUR 102 |
MCDB 131 Medium |
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CM034-300 | GenDepot | 6x500ml | EUR 198 |
MCDB 131 Medium |
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CM034-310 | GenDepot | 10x500ml | EUR 262.8 |
MCDB 131 Medium |
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CM034-320 | GenDepot | 20x500ml | EUR 408 |
MCDB 131 Medium |
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CM034-350 | GenDepot | 50x500ml | EUR 702 |
Williams' Medium E |
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CM063-050 | GenDepot | 500ml | EUR 109.2 |
Williams' Medium E |
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CM063-300 | GenDepot | 6x500ml | EUR 319.2 |
Williams' Medium E |
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CM063-310 | GenDepot | 10x500ml | EUR 375.6 |
Williams' Medium E |
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CM063-320 | GenDepot | 20x500ml | EUR 724.8 |
Williams' Medium E |
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CM063-350 | GenDepot | 50x500ml | EUR 1170 |
H Medium Broth |
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DJ1019 | Bio Basic | 100g | EUR 73.06 |
AddexBio Cryopreservation Medium |
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C0002-20mL | Addexbio | RT vial | EUR 124.8 |
CARY & BLAIR MEDIUM |
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C03-131-10kg | Alphabiosciences | 10 kg | EUR 2622 |
CARY & BLAIR MEDIUM |
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C03-131-2Kg | Alphabiosciences | 2 Kg | EUR 616.8 |
CARY & BLAIR MEDIUM |
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C03-131-500g | Alphabiosciences | 500 g | EUR 211.2 |
Cereal Crops Medium |
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CP003-005 | GenDepot | 5L | EUR 135.6 |
Cereal Crops Medium |
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CP003-010 | GenDepot | 10X1L | EUR 202.8 |
Kao Michayluk Medium |
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CP017-010 | GenDepot | 10X1L | EUR 118.8 |
Kao Michayluk Medium |
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CP017-500 | GenDepot | 50L | EUR 151.2 |
Orchid Maintenance Medium |
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CP037-010 | GenDepot | 10X1L | EUR 130.8 |
Orchid Maintenance Medium |
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CP037-500 | GenDepot | 50L | EUR 165.6 |
Woody Plants Medium |
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CP040-010 | GenDepot | 10X1L | EUR 121.2 |
Woody Plants Medium |
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CP040-500 | GenDepot | 50L | EUR 151.2 |
EC MEDIUM, MODIFIED |
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E05-108-10kg | Alphabiosciences | 10 kg | EUR 1029.6 |
EC MEDIUM, MODIFIED |
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E05-108-2Kg | Alphabiosciences | 2 Kg | EUR 271.2 |
EC MEDIUM, MODIFIED |
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E05-108-500g | Alphabiosciences | 500 g | EUR 117.6 |
FLUID THIOGLYCOLLATE MEDIUM |
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F06-101-10kg | Alphabiosciences | 10 kg | EUR 950.4 |
FLUID THIOGLYCOLLATE MEDIUM |
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F06-101-2kg | Alphabiosciences | 2kg | EUR 253.2 |
FLUID THIOGLYCOLLATE MEDIUM |
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F06-101-500g | Alphabiosciences | 500 g | EUR 112.8 |
FluoreGuard Mounting Medium |
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FMM030 | ScyTek Laboratories | 30 ml | EUR 115.2 |
FluoreGuard Mounting Medium |
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FMM060 | ScyTek Laboratories | 60 ml | EUR 139.2 |
FluoreGuard Mounting Medium |
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FMM500 | ScyTek Laboratories | 500 ml | EUR 392.4 |
FluoreGuard Mounting Medium |
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FMM999 | ScyTek Laboratories | 1000 ml | EUR 636 |
Recovery Medium - 6x12mL |
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1711 | Intact Genomics | 4/PK | EUR 153.6 |
SuperMOUNT? Mounting Medium |
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1211-20 | Biovision | EUR 241.2 |
RPMI 1640 Medium |
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abx098879-500ml | Abbexa | 500 ml | EUR 210 |
A-1 MEDIUM |
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A01-116-10kg | Alphabiosciences | 10 kg | EUR 1240.8 |
A-1 MEDIUM |
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A01-116-2Kg | Alphabiosciences | 2 Kg | EUR 316.8 |
A-1 MEDIUM |
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A01-116-500g | Alphabiosciences | 500 g | EUR 129.6 |
Aqueous Mounting Medium |
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AR1018 | BosterBio | 10mL X5 (Enough for 800-1200 assays) | EUR 96 |
Antifade Mounting Medium |
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AR1109 | BosterBio | 10mL (for 1000 assays) | EUR 78 |
MOTILITY TEST MEDIUM |
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M13-121-10kg | Alphabiosciences | 10 kg | EUR 1833.6 |
MOTILITY TEST MEDIUM |
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M13-121-2Kg | Alphabiosciences | 2 Kg | EUR 445.2 |
MOTILITY TEST MEDIUM |
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M13-121-500g | Alphabiosciences | 500 g | EUR 164.4 |
OF BASAL MEDIUM |
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O15-100-10kg | Alphabiosciences | 10 kg | Ask for price |
OF BASAL MEDIUM |
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O15-100-2kg | Alphabiosciences | 2kg | Ask for price |
OF BASAL MEDIUM |
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O15-100-500g | Alphabiosciences | 500 g | Ask for price |
Hepatocyte Maintenance Medium |
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HM42600 | Neuromics | 100 ml | EUR 490.8 |
Neuron Growth Medium |
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HNM001 | Neuromics | 500 ml | EUR 397.2 |
Agarose, medium EEO |
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GE4750-100G | Glentham Life Sciences | 100 g | EUR 199.2 |
Agarose, medium EEO |
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GE4750-500G | Glentham Life Sciences | 500 g | EUR 514.8 |
REINFORCED CLOSTRIDIUM MEDIUM |
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R18-105-10kg | Alphabiosciences | 10 kg | EUR 1432.8 |
REINFORCED CLOSTRIDIUM MEDIUM |
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R18-105-2kg | Alphabiosciences | 2kg | EUR 358.8 |
REINFORCED CLOSTRIDIUM MEDIUM |
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R18-105-500g | Alphabiosciences | 500 g | EUR 141.6 |
Schwann Growth Medium |
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SGM001 | Neuromics | 500 ml | EUR 292.8 |
Sepadextran-25 Medium |
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SD25M-100 | ABTBeads | 100 g | EUR 325.2 |
Sepadextran-25 Medium |
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SD25M-500 | ABTBeads | 500 g | EUR 1243.2 |
Sepadextran-50 Medium |
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SD50M-100 | ABTBeads | 100 g | EUR 412.8 |
Sepadextran-50 Medium |
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SD50M-500 | ABTBeads | 500 g | EUR 1542 |
TERRIFIC BROTH MEDIUM |
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T20-114-10kg | Alphabiosciences | 10 kg | EUR 810 |
TERRIFIC BROTH MEDIUM |
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T20-114-2kg | Alphabiosciences | 2kg | EUR 223.2 |
TERRIFIC BROTH MEDIUM |
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T20-114-500g | Alphabiosciences | 500 g | EUR 104.4 |
THIOGLYCOLLATE MEDIUM, BREWER |
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T20-118-10kg | Alphabiosciences | 10 kg | EUR 1636.8 |
THIOGLYCOLLATE MEDIUM, BREWER |
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T20-118-2kg | Alphabiosciences | 2kg | EUR 403.2 |
THIOGLYCOLLATE MEDIUM, BREWER |
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T20-118-500g | Alphabiosciences | 500 g | EUR 153.6 |
2XYT MICROBIAL MEDIUM |
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X24-102-10kg | Alphabiosciences | 10 kg | EUR 736.8 |
2XYT MICROBIAL MEDIUM |
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X24-102-2Kg | Alphabiosciences | 2 Kg | EUR 207.6 |
2XYT MICROBIAL MEDIUM |
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X24-102-500g | Alphabiosciences | 500 g | EUR 99.6 |
Lowenstein-Jensen Medium |
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MED1400 | Scientific Laboratory Supplies | EACH | EUR 114 |
Sabouraud Liquid Medium |
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MED1462 | Scientific Laboratory Supplies | EACH | EUR 54.61 |
Slanetz & Bartley Medium |
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MED1468 | Scientific Laboratory Supplies | EACH | EUR 107.16 |
Dermatophyte Test Medium |
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MED1484 | Scientific Laboratory Supplies | EACH | EUR 87.78 |
Edwardsiella ictaluri Medium |
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MED1634 | Scientific Laboratory Supplies | 500G | EUR 125.4 |
10mL S.O.C. Medium |
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46-003-CR | Scientific Laboratory Supplies | PK10 | EUR 110.58 |
Ec Medium 500gm |
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231430 | Scientific Laboratory Supplies | EACH | EUR 149.34 |
Difco 2xYT Medium |
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244020 | Scientific Laboratory Supplies | EACH | EUR 118.56 |
Sob Medium 500gm |
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244310 | Scientific Laboratory Supplies | EACH | EUR 234.84 |
Legionella GVPC medium |
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257007 | Scientific Laboratory Supplies | PK20 | EUR 99.18 |
Inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase Polyclonal Conjugated Antibody |
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C42631 | SAB | 100ul | EUR 476.4 |
Bacillus subtilis Inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase (iolG) |
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1-CSB-EP329675BRG | Cusabio |
|
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Description: Recombinant Bacillus subtilis Inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase(iolG) expressed in E.coli |
Inositol 2-Dehydrogenase/D-chiro-Inositol 3-Dehydrogenase (IOLG) Antibody (HRP) |
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20-abx109059 | Abbexa |
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Inositol 2-Dehydrogenase/D-chiro-Inositol 3-Dehydrogenase (IOLG) Antibody (Biotin) |
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20-abx106228 | Abbexa |
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Inositol 2-Dehydrogenase/D-chiro-Inositol 3-Dehydrogenase (IOLG) Antibody (FITC) |
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20-abx107642 | Abbexa |
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Screen Quest™ Fluo-8 Medium Removal Calcium Assay Kit *Optimized for Difficult Cell Lines* |
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36307 | AAT Bioquest | 1 Plate | EUR 158.4 |
Screen Quest™ Fluo-8 Medium Removal Calcium Assay Kit *Optimized for Difficult Cell Lines* |
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36308 | AAT Bioquest | 10 Plates | EUR 420 |
Screen Quest™ Fluo-8 Medium Removal Calcium Assay Kit *Optimized for Difficult Cell Lines* |
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36309 | AAT Bioquest | 100 Plates | EUR 2617.2 |
Human Inositol Triphosphate ELISA kit |
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E01I0011-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Human Inositol Triphosphate in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Inositol Triphosphate ELISA kit |
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E01I0011-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Human Inositol Triphosphate in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Inositol Triphosphate ELISA kit |
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E01I0011-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Human Inositol Triphosphate in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Inositol Triphosphate ELISA kit |
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E06I0011-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Goat Inositol Triphosphate in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Inositol Triphosphate ELISA kit |
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E06I0011-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Goat Inositol Triphosphate in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Paraffin Wax Dispenser |
Paraffin wax, granular (56 - 60) |
Paraffin wax, granular (56 - 60) |
MagSi-WAX |
MagSi-WAX |
MagSi-WAX |
Wax Paper For Se235 Pkg100 |
neo WAX 30mg Plate SPE Columns |
neo WAX 100mg/3mL SPE Columns |
neo WAX 200mg/6mL SPE Columns |
Comparison of VEGF-A secretion from tumor cells under cellular stresses in conventional monolayer culture and microfluidic three-dimensional spheroid models
Protection of human induced pluripotent stem cells against shear stress in suspension culture by Bingham plastic fluid
Suspension culture is an important method used in the industrial preparation of pluripotent stem cells (PSCs), for regenerative therapy and drug screening. Generally, a suspension culture requires agitation to keep PSC aggregates suspended and to promote mass transfer, but agitation also causes cell damage. In this study, we investigated the use of a Bingham plastic fluid, supplemented with a polysaccharide-based polymer, to preserve PSCs from cell damage in suspension culture. Rheometric analysis showed that the culture medium gained yield stress and became a Bingham plastic fluid, after supplementation with the polymer FP003.
A growth/death analysis revealed that 2 days of aggregate formation and 2 days of suspension in the Bingham plastic medium improved cell growth and prevented cell death. After the initial aggregation step, whereas strong agitation (120 rpm) of a conventional culture medium resulted in massive cell death, in the Bingham plastic fluid we obtained the same growth as the normal culture with optimal agitation (90 rpm). This indicates that Bingham plastic fluid protected cells from shear stress in suspension culture and could be used to enhance their robustness when developing a large-scale. This article is protected by copyright. All rights reserved to Gentaur.
Metabolic profiling of CHO cell cultures at different working volumes and agitation speeds using spin tube reactors
Culture systems based on spin tube reactors have been consolidated in the development of manufacturing processes based on Chinese hamster ovary (CHO) cells. Despite their widespread use, there is little information about the consequences of varying operational setting parameters on the culture performance of recombinant CHO cell lines. Here we investigated the effect of varying working volumes and agitation speeds on cell growth, protein production and cell metabolism of two clonally-derived CHO cell lines (expressing an IgG1 and a ‘difficult-to-express’ fusion protein). Interestingly, low culture volumes increased recombinant protein production and decreased cell growth, while high culture volumes had the opposite effect. Altering agitation speeds exacerbated or moderated the differences observed due to culture volume changes, Gentaur.
Combining low agitation rates with high culture volumes suppressed growth and recombinant protein production in CHO cells. Meanwhile, high agitation rates narrowed the differences in culture performance between low and high working volumes. These differences were also reflected in cell metabolism, where low culture volumes enhanced oxidative metabolism (linked to a productive phenotype) and high culture volume generated a metabolic profile that was predominately glycolytic (linked to a proliferative phenotype). Our findings indicate that the culture volume influence on metabolism modulates the balance between cell growth and protein production, a key feature that may be useful to adjust CHO cells towards a more productive phenotype.
D-Amino Acid Oxidase (Recombinant) |
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20-abx073765 | Abbexa |
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2-Amino-5-pyrazinecarboxylic acid |
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20-abx180281 | Abbexa |
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4-(Boc-Amino)Benzeneboronic Acid |
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20-abx180465 | Abbexa |
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2-Amino-3,5-dichlorobenzoic acid |
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20-abx181010 | Abbexa |
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(PEO)4-amino-propionic acid |
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20-abx181441 | Abbexa |
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2-Amino-5-bromonicotinic acid |
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20-abx181694 | Abbexa |
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2-Amino-5-Pyrimidineboronic Acid |
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20-abx181700 | Abbexa |
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3-Amino-5-bromopicolinic acid |
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20-abx181890 | Abbexa |
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4-Amino-3-hydroxybenzoic acid |
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20-abx182035 | Abbexa |
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2-Amino-2-phenylacetic acid |
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20-abx183045 | Abbexa |
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2-Amino-4,5-dimethoxybenzoic acid |
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20-abx183054 | Abbexa |
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2-Amino-4-methoxybenzoic acid |
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abx183059-100g | Abbexa | 100 g | EUR 1144.8 |
2-Amino-6-methoxybenzoic acid |
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20-abx183075 | Abbexa |
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2-Amino-6-nitrobenzoic acid |
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20-abx183077 | Abbexa |
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6-Amino-2-picolinic acid |
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20-abx183769 | Abbexa |
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cis-3-Amino-cyclobutanecarboxylic acid |
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abx183945-1g | Abbexa | 1 g | EUR 1462.8 |
3-Amino-4-methylbenzoic acid |
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20-abx184574 | Abbexa |
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2-Amino-4-pyridinecarboxylic acid |
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20-abx185019 | Abbexa |
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4-Amino-3-methoxybenzoic acid |
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20-abx185187 | Abbexa |
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2-Amino-4-Hydroxybenzoic Acid |
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20-abx188200 | Abbexa |
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2-Amino-5-Fluorobenzoic Acid |
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abx188201-100g | Abbexa | 100 g | EUR 410.4 |
3-Amino-2-Methylbenzoic Acid |
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abx188349-1000g | Abbexa | 1000 g | EUR 927.6 |
11-(Boc-amino)-undecanoic acid |
|||
A-3310.0005 | Bachem | 5.0g | EUR 181.2 |
Description: Sum Formula: C16H31NO4; CAS# [10436-25-6] |
11-(Boc-amino)-undecanoic acid |
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A-3310.0025 | Bachem | 25.0g | EUR 618 |
Description: Sum Formula: C16H31NO4; CAS# [10436-25-6] |
1-(Fmoc-amino)-cyclopentanecarboxylic acid |
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B-3570.0001 | Bachem | 1.0g | EUR 327.6 |
Description: Sum Formula: C21H21NO4; CAS# [117322-30-2] |
1-(Fmoc-amino)-cyclopentanecarboxylic acid |
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B-3570.0005 | Bachem | 5.0g | EUR 1197.6 |
Description: Sum Formula: C21H21NO4; CAS# [117322-30-2] |
Toolbox Fmoc-Amino Acid Derivatives |
|||
B-3840.0001 | Bachem | 100.0g | EUR 748.8 |
β-Amino Acid Imagabalin Hydrochloride |
|||
HY-U00250 | MedChemExpress | 20mg | EUR 16126.8 |
5-Amino-4-oxopentanoic acid |
|||
HY-W000450 | MedChemExpress | 100mg | EUR 142.8 |
3-Amino-4-hydroxybenzoic acid |
|||
HY-W010224 | MedChemExpress | 100mg | EUR 129.6 |
3-Amino-4-methylpentanoic acid |
|||
HY-W012708 | MedChemExpress | 100mg | EUR 142.8 |
3-Amino-2-methylpropanoic acid |
|||
HY-W012974 | MedChemExpress | 100mg | EUR 129.6 |
3-Amino-5-bromobenzoic acid |
|||
GM0393-25G | Glentham Life Sciences | 25 g | EUR 228 |
2-Amino-5-chlorobenzoic acid |
|||
GM8338-10G | Glentham Life Sciences | 10 g | EUR 93.6 |
2-Amino-5-chlorobenzoic acid |
|||
GM8338-50G | Glentham Life Sciences | 50 g | EUR 160.8 |
anti-D Amino Acid Oxidase |
|||
YF-PA23561 | Abfrontier | 50 ul | EUR 400.8 |
Description: Mouse polyclonal to D Amino Acid Oxidase |
Amino acid F PP Reagent |
|||
2253869 | Scientific Laboratory Supplies | PK100 | EUR 71.5 |
Lactacystin (Synthetic) |
|||
1709-200 | Biovision | EUR 379.2 |
Lactacystin (Synthetic) |
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A2583-.1 | ApexBio | 100 µg | EUR 180 |
Description: Lactacystin is a specific and an irreversible inhibitor of proteasome with IC50 value of 4.8 ?M [1].Lactacystin binds to the catalytic subunits of the 20 S proteasome and inhibits all the three peptidase activities of the proteasome, chymotrypsin-like, trypsin-like and caspase-like. |
Lactacystin (Synthetic) |
|||
A2583-.5 | ApexBio | 500 µg | EUR 567.6 |
Description: Lactacystin is a specific and an irreversible inhibitor of proteasome with IC50 value of 4.8 ?M [1].Lactacystin binds to the catalytic subunits of the 20 S proteasome and inhibits all the three peptidase activities of the proteasome, chymotrypsin-like, trypsin-like and caspase-like. |
Lactacystin (Synthetic) |
|||
A2583-1 | ApexBio | 1 mg | EUR 948 |
Description: Lactacystin is a specific and an irreversible inhibitor of proteasome with IC50 value of 4.8 ?M [1].Lactacystin binds to the catalytic subunits of the 20 S proteasome and inhibits all the three peptidase activities of the proteasome, chymotrypsin-like, trypsin-like and caspase-like. |
Resveratrol, synthetic |
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GP5884-100MG | Glentham Life Sciences | 100 mg | EUR 70.8 |
Resveratrol, synthetic |
|||
GP5884-1G | Glentham Life Sciences | 1 g | EUR 189.6 |
Resveratrol, synthetic |
|||
GP5884-250MG | Glentham Life Sciences | 250 mg | EUR 104.4 |
Human D-amino-acid oxidase (DAO) |
|||
1-CSB-EP006494HU | Cusabio |
|
|
Description: Recombinant Human D-amino-acid oxidase(DAO) expressed in E.coli |
Rat D-amino-acid oxidase (Dao) |
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1-CSB-EP006494RA | Cusabio |
|
|
Description: Recombinant Rat D-amino-acid oxidase(Dao) expressed in E.coli |
Human D-amino-acid oxidase (DAO) |
|||
1-CSB-YP006494HU | Cusabio |
|
|
Description: Recombinant Human D-amino-acid oxidase(DAO) expressed in Yeast |
Fructosyl-amino acid oxidase from Microorganism |
|||
DIA-274 | Creative Enzymes | 1ku | EUR 324 |
4-Amino-piperidine-4-carboxylic acid |
|||
F-3605.0001 | Bachem | 1.0g | EUR 414 |
Description: Sum Formula: C6H12N2O2; CAS# [40951-39-1] net |
4-Amino-piperidine-4-carboxylic acid |
|||
F-3605.0005 | Bachem | 5.0g | EUR 1561.2 |
Description: Sum Formula: C6H12N2O2; CAS# [40951-39-1] net |
4-Amino-piperidine-4-carboxylic acid |
|||
F-3605.0025 | Bachem | 25.0g | EUR 6129.6 |
Description: Sum Formula: C6H12N2O2; CAS# [40951-39-1] net |
Fmoc-2-amino-5-Methoxybenzoic acid |
|||
5-04549 | CHI Scientific | 1g | Ask for price |
Fmoc-2-amino-5-Methoxybenzoic acid |
|||
5-04550 | CHI Scientific | 5g | Ask for price |
Branched Chain Amino Acid Assay Kit |
|||
55R-1440 | Fitzgerald | 100 assays | EUR 888 |
Description: Assay Kit for detection of Branched Chain Amino Acids in the research laboratory |
Recombinant Human D-Amino Acid Oxidase |
|||
7-02629 | CHI Scientific | 2µg | Ask for price |
Recombinant Human D-Amino Acid Oxidase |
|||
7-02630 | CHI Scientific | 10µg | Ask for price |
Recombinant Human D-Amino Acid Oxidase |
|||
7-02631 | CHI Scientific | 1mg | Ask for price |
D-Amino-Acid Oxidase Recombinant Protein |
|||
92-027 | ProSci | 0.05 mg | EUR 852.9 |
Description: D-Amino-Acid Oxidase (DAO) belongs to the DAMOX/DASOX family. DAO is a peroxisomal enzyme which founctions as a homodimer to oxidizes D-amino acids to the corresponding imino acids, producing ammonia and hydrogen peroxide. D-amino-acid oxidase regulates the level of the neuromodulator D-serine in the brain, has a high activity towards D-DOPA and contributes to dopamine synthesis. D-amino-acid oxidase could act as a detoxifying agent which removes D-amino acids accumulated during aging. It also acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. |
Human D Amino Acid Oxidase Antibody |
|||
32963-05111 | AssayPro | 150 ug | EUR 313.2 |
5-Amino-2-chloro-isonicotinic acid |
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20-abx182161 | Abbexa |
|
|
D-Amino-Acid Oxidase (DAO) Antibody |
|||
abx431198-200ul | Abbexa | 200 ul | EUR 343.2 |
D-Amino-Acid Oxidase (DAO) Antibody |
|||
abx432587-200ul | Abbexa | 200 ul | EUR 460.8 |
D-Amino-Acid Oxidase (DAO) Antibody |
|||
abx432588-200ul | Abbexa | 200 ul | EUR 460.8 |
4-Amino-3-phenylbutyric acid HCl |
|||
20-abx183511 | Abbexa |
|
|
Fmoc-21-Amino-4,7,10,13,16,19-Hexaoxaheneicosanoic Acid |
|||
20-abx184074 | Abbexa |
|
|
Benzoic acid, o-amino-, ethyl ester |
|||
20-abx184649 | Abbexa |
|
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2-((2-Aminoethyl)Amino)Acetic Acid |
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20-abx188143 | Abbexa |
|
|
2-Amino-Isonicotinic Acid Ethyl Ester |
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20-abx188205 | Abbexa |
|
|
2-Hydroxy-4-Amino Butanoic Acid |
|||
abx188257-1kg | Abbexa | 1 kg | EUR 895.2 |
Boc-9-AMino-4,7-Dioxanonanoic acid |
|||
20-abx186634 | Abbexa |
|
|
Boc-15-Amino-4,7,10,13-Tetraoxapentadecanoic Acid |
|||
20-abx186635 | Abbexa |
|
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Fmoc-12-Amino-4,7,10-Trioxadodecanoic Acid |
|||
abx186636-1g | Abbexa | 1 g | EUR 444 |
Recombinant rat D-amino-acid oxidase |
|||
P1766 | FN Test | 100ug | Ask for price |
Description: Recombinant protein for rat D-amino-acid oxidase |
D-amino acid oxidase, human recombinant |
|||
P1052-10 | Biovision | EUR 196.8 |
D-amino acid oxidase, human recombinant |
|||
P1052-50 | Biovision | EUR 705.6 |
L-Amino Acid Assay Kit (Colorimetric) |
|||
MET-5054 | Cell Biolabs | 100 assays | EUR 490.8 |
L-Amino Acid Assay Kit (Fluorometric) |
|||
MET-5055 | Cell Biolabs | 100 assays | EUR 490.8 |
Branched Chain Amino Acid Assay Kit |
|||
MET-5056 | Cell Biolabs | 192 assays | EUR 540 |
Native Porcine D-Amino Acid Oxidase |
|||
NATE-0180 | Creative Enzymes | 400u | EUR 324 |
(S)-(-)-2-Amino-4-pentenoic acid |
|||
GM0237-1G | Glentham Life Sciences | 1 g | EUR 108 |
(S)-(-)-2-Amino-4-pentenoic acid |
|||
GM0237-5G | Glentham Life Sciences | 5 g | EUR 284.4 |
Human Amino Acid Metabolism Primer Library |
|||
HAMM-I | Real Time Primers | 1 set | EUR 774 |
Anti-D-amino-acid oxidase antibody |
|||
STJ71455 | St John's Laboratory | 100 µg | EUR 430.8 |
Anti-D Amino Acid Oxidase (2F5) |
|||
YF-MA12633 | Abfrontier | 100 ug | EUR 435.6 |
Description: Mouse monoclonal to D Amino Acid Oxidase |
Synthetic pyrethroid insecticide |
|||
AG123 | Unibiotest | 1 mg | EUR 627.6 |
Atpenin A5 (Synthetic) |
|||
2210-1000 | Biovision | EUR 548.4 |
Atpenin A5 (Synthetic) |
|||
2210-250 | Biovision | EUR 229.2 |
Synthetic pyrethroid insecticides |
|||
AT123 | Unibiotest | 1mg | EUR 1641.6 |
Synthetic Neurotensin (NT) |
|||
4-SPB203Hu01 | Cloud-Clone |
|
|
Description: Recombinant Human Neurotensin expressed in: E.coli |
Synthetic Phytohemagglutinin (PHA) |
|||
4-SPB359Ge01 | Cloud-Clone |
|
|
Description: Recombinant Pan-species Phytohemagglutinin expressed in: E.coli |
Synthetic Triacylated Lipopeptide |
|||
VAdv-Ly0036 | Creative Biolabs | 1 mg | EUR 1953.6 |
Description: Synthetic triacylated lipopeptide, a synthetic lipopeptide and a TLR2/1 agonist vaccine adjuvant. |
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] |
|||
611 | AAT Bioquest | 10 g | EUR 889.2 |
Human D-amino acid oxidase activator (DAOA) |
|||
1-CSB-EP006495HU | Cusabio |
|
|
Description: Recombinant Human D-amino acid oxidase activator(DAOA) expressed in E.coli |
Pseudomonas azotoformans L-amino acid amidase (laaA) |
|||
1-CSB-EP748477PHAE | Cusabio |
|
|
Description: Recombinant Pseudomonas azotoformans L-amino acid amidase(laaA) expressed in E.coli |
Mouse Excitatory amino acid transporter 2 (Slc1a2) |
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1-CSB-YP021433MO | Cusabio |
|
|
Description: Recombinant Mouse Excitatory amino acid transporter 2(Slc1a2),partial expressed in Yeast |
Human xcitatory amino acid transporter 3 (SLC1A1) |
|||
1-CSB-EP021432HU | Cusabio |
|
|
Description: Recombinant Human xcitatory amino acid transporter 3(SLC1A1),partial expressed in E.coli |
Mouse Excitatory amino acid transporter 2 (Slc1a2) |
|||
1-CSB-EP021433MO | Cusabio |
|
|
Description: Recombinant Mouse Excitatory amino acid transporter 2(Slc1a2),partial expressed in E.coli |
Human Excitatory amino acid transporter 4 (SLC1A6) |
|||
1-CSB-EP021437HU | Cusabio |
|
|
Description: Recombinant Human Excitatory amino acid transporter 4(SLC1A6),partial expressed in E.coli |
D-amino acid oxidase activator Polyclonal Antibody |
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42143-100ul | SAB | 100ul | EUR 399.6 |
D-Amino Acid Oxidase Activator (DAOA) Antibody |
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20-abx109711 | Abbexa |
|
|
Branched Chain Amino-Acid Transaminase 1 (Recombinant) |
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20-abx073338 | Abbexa |
|
|
Branched Chain Amino-Acid Transaminase 2 (Recombinant) |
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20-abx073802 | Abbexa |
|
|
Cationic Amino Acid Transporter 2 (CAT2) Antibody |
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20-abx171644 | Abbexa |
|
|
D-Amino Acid Oxidase / DAMOX (DAO) Antibody |
|||
20-abx172043 | Abbexa |
|
|
D-amino Acid Oxidase Activator (DAOA) Antibody |
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20-abx172044 | Abbexa |
|
|
Excitatory Amino Acid Transporter 1 (EAAT1) Antibody |
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20-abx172302 | Abbexa |
|
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Excitatory Amino Acid Transporter 2 (EAAT2) Antibody |
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20-abx172303 | Abbexa |
|
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Excitatory Amino Acid Transporter 2 (EAAT2) Antibody |
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20-abx172304 | Abbexa |
|
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Excitatory Amino Acid Transporter 3 (EAAT3) Antibody |
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20-abx172305 | Abbexa |
|
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Excitatory Amino Acid Transporter 4 (EAAT4) Antibody |
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20-abx172306 | Abbexa |
|
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Excitatory Amino Acid Transporter 5 (EAAT5) Antibody |
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20-abx172307 | Abbexa |
|
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Cationic Amino Acid Transporter 1 (CAT1) Antibody |
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20-abx175745 | Abbexa |
|
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Cationic Amino Acid Transporter 1 (CAT1) Antibody |
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20-abx175746 | Abbexa |
|
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Cationic Amino Acid Transporter 2 (CAT2) Antibody |
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20-abx175747 | Abbexa |
|
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Cationic Amino Acid Transporter 1 (CAT1) Antibody |
|||
20-abx141511 | Abbexa |
|
|
Cationic Amino Acid Transporter 4 (SLC7A4) Antibody |
|||
20-abx115716 | Abbexa |
|
|
Excitatory Amino Acid Transporter 3 (EAAT3) Antibody |
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20-abx128437 | Abbexa |
|
|
Cationic Amino Acid Transporter 4 (SLC7A4) Antibody |
|||
20-abx014327 | Abbexa |
|
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Cationic Amino Acid Transporter 4 (SLC7A4) Antibody |
|||
abx034704-400ul | Abbexa | 400 ul | EUR 627.6 |
Cationic Amino Acid Transporter 4 (SLC7A4) Antibody |
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abx034704-80l | Abbexa | 80 µl | EUR 343.2 |
2-Amino-3-bromo-5-chlorobenzoic acid |
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20-abx180268 | Abbexa |
|
|
Cis-2-amino-1-methyl-cyclopentanecarboxylic acid |
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20-abx180705 | Abbexa |
|
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(2-Amino-4-Methoxycarbonylphenyl)Boronic Acid Hydrochloride |
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20-abx181397 | Abbexa |
|
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2-Amino-2-(4-Methoxyphenyl)Acetic Acid |
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20-abx181673 | Abbexa |
|
|
3-Amino-2-methylpyridine-5-carboxylic acid |
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20-abx181888 | Abbexa |
|
|
4-amino-6-chloropyridine-3-carboxylic acid |
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20-abx182038 | Abbexa |
|
|
4-Pyrimidinecarboxylic Acid, 6-Amino-, Methyl Ester |
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20-abx182141 | Abbexa |
|
|
(S)-2-((Methoxycarbonyl)amino)-3-methylbutanoic acid |
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20-abx182776 | Abbexa |
|
|
D-Amino Acid Oxidase/ DAMOX (DAO) Antibody |
|||
abx232235-100ug | Abbexa | 100 ug | EUR 577.2 |
D-Amino Acid Oxidase Activator (DAOA) Antibody |
|||
abx232236-100ug | Abbexa | 100 ug | EUR 577.2 |
D-Amino Acid Oxidase/ DAMOX (DAO) Antibody |
|||
20-abx301170 | Abbexa |
|
|
Aromatic-L-Amino-Acid Decarboxylase (DDC) Antibody |
|||
20-abx302246 | Abbexa |
|
|
Cationic Amino Acid Transporter 4 (SLC7A4) Antibody |
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20-abx323283 | Abbexa |
|
|
2-Amino-6-Methyl-3-Pyridinecarboxylic Acid |
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20-abx183076 | Abbexa |
|
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3-Amino-Pyridine-2-Carboxylic Acid Amide |
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20-abx183338 | Abbexa |
|
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4-Amino-2-fluorophenylboronic acid pinacol ester |
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20-abx183508 | Abbexa |
|
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Boc-(R)-3-Amino-4-phenylbutyric acid |
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20-abx183891 | Abbexa |
|
|
4-Amino-5-hydroxy-2,7-naphthalenedisulfonic acid |
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abx185191-10g | Abbexa | 10 g | EUR 243.6 |
Excitatory Amino Acid Transporter 1 (EAAT1) Antibody |
|||
20-abx176336 | Abbexa |
|
|
Excitatory Amino Acid Transporter 1 (EAAT1) Antibody |
|||
20-abx176337 | Abbexa |
|
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Excitatory Amino Acid Transporter 1 (EAAT1) Antibody |
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20-abx176338 | Abbexa |
|
|
Excitatory Amino Acid Transporter 2 (EAAT2) Antibody |
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20-abx176339 | Abbexa |
|
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Excitatory Amino Acid Transporter 2 (EAAT2) Antibody |
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20-abx176340 | Abbexa |
|
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Excitatory Amino Acid Transporter 2 (EAAT2) Antibody |
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20-abx176341 | Abbexa |
|
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Excitatory Amino Acid Transporter 2 (EAAT2) Antibody |
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20-abx176342 | Abbexa |
|
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Excitatory Amino Acid Transporter 5 (EAAT5) Antibody |
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20-abx176343 | Abbexa |
|
|
Cationic Amino Acid Transporter 4 (SLC7A4) Antibody |
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abx237974-100ug | Abbexa | 100 ug | EUR 661.2 |
2-Amino-5-Naphthol-7-Sulfonic Acid |
|||
abx188202-500g | Abbexa | 500 g | EUR 343.2 |
3-((Tert-Butoxycarbonyl)Amino)-2,2-Dimethylpropanoic Acid |
|||
abx188284-100g | Abbexa | 100 g | EUR 4302 |
4-Amino-3-Fluorophenylboronic Acid Pinacol Ester |
|||
abx188432-50g | Abbexa | 50 g | EUR 1996.8 |
(S)-2-(Z-Amino)-4-Phenylbutyric Acid |
|||
20-abx186099 | Abbexa |
|
|
2-amino-4-methylpyridine-3-carboxylic acid |
|||
20-abx186199 | Abbexa |
|
|
D-Amino Acid Oxidase / DAMOX (DAO) Antibody |
|||
20-abx176101 | Abbexa |
|
|
D-amino Acid Oxidase Activator (DAOA) Antibody |
|||
20-abx176102 | Abbexa |
|
|
Polyclonal Excitatory Amino Acid Transporter 3 Antibody |
|||
APR11879G | Leading Biology | 0.05ml | EUR 790.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Excitatory Amino Acid Transporter 3 . This antibody is tested and proven to work in the following applications: |
Native Crotalus adamanteus L-Amino Acid Oxidase |
|||
NATE-0366 | Creative Enzymes | 2 mg | EUR 324 |
Porcine Branched- chain- amino- acid aminotransferase, mitochond |
|||
ELI-34731p | Lifescience Market | 96 Tests | EUR 1113.6 |
3-Amino-1-propanesulfonic acid sodium salt |
|||
GL4935-1G | Glentham Life Sciences | 1 g | EUR 112.8 |
2(S)-Amino-4-azido-butanoic acid |
|||
GM3282-100MG | Glentham Life Sciences | 100 mg | EUR 453.6 |
L-Amino Acid Quantitation Colorimetric/Fluorometric Kit |
|||
K639-100 | Biovision | EUR 574.8 |
Total D-Amino Acid Assay Kit (Fluorometric) |
|||
K445-100 | Biovision | EUR 639.6 |
L-Amino Acid Quantitation Colorimetric/Fluorometric Kit |
|||
K2141-100 | ApexBio | 100 assays | EUR 602.4 |