Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts

November 12, 2020
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Culture of Intestinal Epithelial Cell Monolayers and Their Use in Multiplex Macromolecular Permeability Assays for In Vitro Analysis of Tight Junction Size Selectivity

 

Tight junctions form a selectively permeable barrier that limits paracellular flux across epithelial-lined surfaces. Small molecules (less than ∼8 Å diameter) can traverse the junction via the size- and charge-selective, high-conductance pore pathway. In contrast, the low-conductance leak pathway accommodates larger macromolecules (up to ∼100 Å diameter) and is not charge-selective. Flux across the tight junction-independent, high-conductance, non-selective, unrestricted pathway occurs at sites of epithelial damage.

 

Cytokines can regulate each of these pathways, but commonly used measures of barrier function cannot discriminate between tight junction regulation and epithelial damage. This article describes methods for culturing intestinal epithelial cell monolayers and assessing the impact of cytokine treatment on leak and unrestricted pathway permeabilities. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Generation and culture of cell monolayers in Transwells Basic Protocol 2: Assessment of cytokine (IFNγ and TNF) treatment effects on barrier function Support Protocol: Immunofluorescent staining of monolayers Basic Protocol 3: Multiplex flux assay.

Paraffin Wax
Paraffin Wax Trimmer
Paraffin Wax Dispenser - EACH
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
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Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts

Introduction: Biomphalaria snails may display varying levels of susceptibility to Schistosoma mansoni infection. We have been developing an in vitro model to study the interaction between the snail and the parasite, using tissue-derived cell cultures from Biomphalaria.
Methods: The digestive gland- and kidney-derived cells from primary cultures of resistant (B. tenagophila Taim) and susceptible (B. tenagophila HM and B. glabrata BH) strains of Biomphalaria were exposed to S. mansoni sporocysts.
Results: S. mansoni sporocysts were surrounded and encapsulated exclusively by cells derived from the digestive gland (DG) of B. tenagophila Taim. The process was followed by a marked decrease in the number of free sporocysts in the culture medium. The morphological characteristics of DG-derived cells in culture have been described.
Conclusions: Cells derived from DG (but not SK) primary cultures of B. tenagophila Taim may participate in S. mansoni sporocyst control.
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eaaci2015

RPMI 1640 Medium w/o L-Glutamine, Inositol (Powder)
MBS652997-50L 50L
EUR 860

RPMI 1640 Medium w/o L-Glutamine, Inositol (Powder)
MBS652997-5x100L 5x100L
EUR 6570

Inositol Triphosphate (IP3) Magnetic Luminex Assay Kit
LKU603314-96T 96T
EUR 1125.9

Myo Inositol Oxygenase (MIOX) Magnetic Luminex Assay Kit
LKU605077-96T 96T
EUR 966

Assay Medium 2C
78544-1 100 ml
EUR 120
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Assay Medium 2C
78544-2 500 ml
EUR 600
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Assay Medium 7A
78673-1 100 ml
EUR 120
Description: This liquid Assay Medium is optimized for use with BPS Bioscience cell-based assay  

Assay Medium 7A
78673-2 500 ml
EUR 600
Description: This liquid Assay Medium is optimized for use with BPS Bioscience cell-based assay  

Assay Medium 1C
78674-1 100 ml
EUR 120
Description: This liquid Assay Medium is optimized for use with BPS Bioscience cell-based assay.

Assay Medium 1C
78674-2 500 ml
EUR 600
Description: This liquid Assay Medium is optimized for use with BPS Bioscience cell-based assay.

Assay Medium 1B
79617-1 100 ml
EUR 135
Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• TGF/SMAD Signaling Pathway SBE Reporter - HEK293 Cell Line #60653 _x000D_• AP-1 Reporter - HEK293 Recombinant Cell Line (JNK signaling pathway) #60405

Assay Medium 1B
79617-2 500 ml
EUR 675
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• TGF/SMAD Signaling Pathway SBE Reporter - HEK293 Cell Line #60653 _x000D_• AP-1 Reporter - HEK293 Recombinant Cell Line (JNK signaling pathway) #60405

Assay Medium 2B
79619-1 100 ml
EUR 120
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• Foxp3 Reporter (Luc) - Jurkat Recombinant Cell Line -#60628

Assay Medium 2B
79619-2 500 ml
EUR 600
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• Foxp3 Reporter (Luc) - Jurkat Recombinant Cell Line -#60628

Assay Medium 5A
79620-1 100 ml
EUR 120
Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience cell lines.

Assay Medium 5A
79620-2 500 ml
EUR 600
Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience cell lines.

Assay Medium 2A
79621-1 100 ml
EUR 120
Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• ADCC Bioassay Effector Cell F variant (Low Affinity) - Jurkat Recombinant Cell Line #60540 _x000D_• ADCC Bioassay Effector Cell V variant (High Affinity) - Jurkat Recombinant Cell Line #60541

Assay Medium 2A
79621-2 500 ml
EUR 600
Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• ADCC Bioassay Effector Cell F variant (Low Affinity) - Jurkat Recombinant Cell Line #60540 _x000D_• ADCC Bioassay Effector Cell V variant (High Affinity) - Jurkat Recombinant Cell Line #60541

Assay Medium 2C
79623-1 100 ml
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Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• TIGIT / NFAT Reporter - Jurkat Recombinant Cell Line - #60538_x000D_• CD155/TCR Activator - CHO Recombinant Cell Line -#60548

Assay Medium 2C
79623-2 500 ml
EUR 600
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• TIGIT / NFAT Reporter - Jurkat Recombinant Cell Line, #60538 • CD155/TCR Activator - CHO Recombinant Cell Line, #60548

Assay Medium 7B
79718-1 100 ml
EUR 120
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Assay Medium 7B
79718-2 500 ml
EUR 600
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Assay Medium 1A
79805-1 100 ml
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Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including TrkA / SRE Reporter - HEK293 Recombinant Cell Line (#79798)

Assay Medium 1A
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Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including TrkA / SRE Reporter - HEK293 Recombinant Cell Line (#79798)

Pyridoxine Assay Medium
10666 100 Gms
EUR 29.08
Description: Part D

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79816-1 100 ml
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79816-2 500 ml
EUR 600
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LKU600468-96T 96T
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LKU600474-96T 96T
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Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) Magnetic Luminex Assay Kit
LKU600478-96T 96T
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PD-1 Cell-Based Assay Medium
60189 100 ml
EUR 120
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Antibiotic Assay Medium No. 1 (Seed Agar)
87878 100 Gms
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Antibiotic Assay Medium No. 1 (Seed Agar)
87878-1 500 Gms
EUR 39.34
Description: Part D

Antibiotic Assay Medium No. 5 (Streptomycin Assay Agar w/ Yeast Extract)
70197 100 Gms
EUR 9.41
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Antibiotic Assay Medium No. 5 (Streptomycin Assay Agar w/ Yeast Extract)
70197-1 500 Gms
EUR 41.05
Description: Part D

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540221 100.0g
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MBS576494-100mg 100mg
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Inositol
MBS576494-200mg 200mg
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MBS576494-500mg 500mg
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MBS641039-1kg 1kg
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MBS641039-500g 500g
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Antibiotic Assay Medium No. 9 (Polymyxin Base Agar)
52812 100 Gms
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Antibiotic Assay Medium No. 9 (Polymyxin Base Agar)
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EUR 33.36
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Antibiotic Assay Medium No. 10 (Polymyxin Seed Agar)
26751 100 Gms
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Antibiotic Assay Medium No. 8 (Base Agar w/Low pH)
24301 100 Gms
EUR 9.41
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Antibiotic Assay Medium No. 8 (Base Agar w/Low pH)
24301-1 500 Gms
EUR 41.05
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Inositol 98%
I02160 100G
EUR 193.52
Description: CAS N° 87-89-8

Antibiotic Assay Medium No. 2 (Base Agar, Penassay Base Agar))
50741 100 Gms
EUR 9.41
Description: Part D

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50741-1 500 Gms
EUR 41.05
Description: Part D

Inositol oxygenase
AP85281 1mg
EUR 2640

Inositol oxygenase
AP85294 1mg
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Inositol oxygenase
AP85305 1mg
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Inositol oxygenase
AP85464 1mg
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Inositol oxygenase
AP85482 1mg
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Inositol nicotinate
HY-122365 100 mg
EUR 86.58
Description: Inositol nicotinate, with vasodilatory effect, is used in the study of Peripheral arterial disease (PAD)[1].

Inositol nicotinate
MBS3607726-10mg 10mg
EUR 200

Inositol nicotinate
MBS3607726-25mg 25mg
EUR 220

Inositol nicotinate
MBS3607726-5x25mg 5x25mg
EUR 665

Inositol nicotinate
MBS5750609-100mg 100mg
EUR 235

Inositol nicotinate
MBS5750609-200mg 200mg
EUR 285

Inositol nicotinate
MBS5750609-25mg 25mg
EUR 155

Inositol nicotinate
MBS5750609-500mg 500mg
EUR 465

Inositol nicotinate
MBS5750609-50mg 50mg
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98211 25 Gms
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Description: 643-12-9

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Inositol monophosphatase 1
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AP84290 1mg
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Inositol monophosphatase 1
AP84706 1mg
EUR 2640

Inositol monophosphatase 1
AP84712 1mg
EUR 2640

Inositol monophosphatase 1
AP84735 1mg
EUR 2640

Inositol monophosphatase 2
AP84768 1mg
EUR 2640

Inositol monophosphatase 1
AP84894 1mg
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Inositol monophosphatase 3
MBS7042936-002mg 0.02mg
EUR 1675

Inositol monophosphatase 3
MBS7042936-01mg 0.1mg
EUR 2635

Inositol monophosphatase 3
MBS7042936-5x01mg 5x0.1mg
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Inositol monophosphatase 3
MBS7042937-002mg 0.02mg
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Inositol monophosphatase 3
MBS7042937-01mg 0.1mg
EUR 2635

Inositol monophosphatase 3
MBS7042937-5x01mg 5x0.1mg
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Inositol monophosphatase 3
MBS7042938-002mg 0.02mg
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Inositol monophosphatase 3
MBS7042938-01mg 0.1mg
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Inositol monophosphatase 3
MBS7042938-5x01mg 5x0.1mg
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IPMK (Inositol 1,3,4,6-tetrakisphosphate 5-kinase, Inositol Polyphosphate Multikinase, IMPK)
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EUR 695

IPMK (Inositol 1,3,4,6-tetrakisphosphate 5-kinase, Inositol Polyphosphate Multikinase, IMPK)
MBS630540-5x02mL 5x0.2mL
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IPMK (Inositol Polyphosphate Multikinase, Inositol 1,3,4,6-tetrakisphosphate 5-kinase, IMPK)
MBS628695-01mg 0.1mg
EUR 665

IPMK (Inositol Polyphosphate Multikinase, Inositol 1,3,4,6-tetrakisphosphate 5-kinase, IMPK)
MBS628695-5x01mg 5x0.1mg
EUR 2850

GeneAssays Custom Gene Expression Genotyping Assay 20X Mix, Medium
41-1141-02 3200x 20 µL Reaction Ask for price

α-Glycerophosphoryl Inositol
G598770 100mg
EUR 17000
Description: 2816-11-7

IPMK (Inositol Polyphosphate Multikinase, Inositol 1,3,4,6-tetrakisphosphate 5-kinase, IMPK) (AP)
MBS6483435-01mL 0.1mL
EUR 920

IPMK (Inositol Polyphosphate Multikinase, Inositol 1,3,4,6-tetrakisphosphate 5-kinase, IMPK) (AP)
MBS6483435-5x01mL 5x0.1mL
EUR 3990

IPMK (Inositol Polyphosphate Multikinase, Inositol 1,3,4,6-tetrakisphosphate 5-kinase, IMPK) APC
MBS6483436-01mL 0.1mL
EUR 920

IPMK (Inositol Polyphosphate Multikinase, Inositol 1,3,4,6-tetrakisphosphate 5-kinase, IMPK) APC
MBS6483436-5x01mL 5x0.1mL
EUR 3990

IPMK (Inositol Polyphosphate Multikinase, Inositol 1,3,4,6-tetrakisphosphate 5-kinase, IMPK) (FITC)
MBS6483438-01mL 0.1mL
EUR 920

IPMK (Inositol Polyphosphate Multikinase, Inositol 1,3,4,6-tetrakisphosphate 5-kinase, IMPK) (FITC)
MBS6483438-5x01mL 5x0.1mL
EUR 3990

IPMK (Inositol Polyphosphate Multikinase, Inositol 1,3,4,6-tetrakisphosphate 5-kinase, IMPK) (HRP)
MBS6483439-01mL 0.1mL
EUR 920

IPMK (Inositol Polyphosphate Multikinase, Inositol 1,3,4,6-tetrakisphosphate 5-kinase, IMPK) (HRP)
MBS6483439-5x01mL 5x0.1mL
EUR 3990

IPMK (Inositol Polyphosphate Multikinase, Inositol 1,3,4,6-tetrakisphosphate 5-kinase, IMPK) (PE)
MBS6483445-01mL 0.1mL
EUR 920

IPMK (Inositol Polyphosphate Multikinase, Inositol 1,3,4,6-tetrakisphosphate 5-kinase, IMPK) (PE)
MBS6483445-5x01mL 5x0.1mL
EUR 3990

IPMK (Inositol 1,3,4,6-tetrakisphosphate 5-kinase, Inositol Polyphosphate Multikinase, IMPK) (AP)
MBS6483446-02mL 0.2mL
EUR 980

IPMK (Inositol 1,3,4,6-tetrakisphosphate 5-kinase, Inositol Polyphosphate Multikinase, IMPK) (AP)
MBS6483446-5x02mL 5x0.2mL
EUR 4250

IPMK (Inositol 1,3,4,6-tetrakisphosphate 5-kinase, Inositol Polyphosphate Multikinase, IMPK) (APC)
MBS6483447-02mL 0.2mL
EUR 980

IPMK (Inositol 1,3,4,6-tetrakisphosphate 5-kinase, Inositol Polyphosphate Multikinase, IMPK) (APC)
MBS6483447-5x02mL 5x0.2mL
EUR 4250

IPMK (Inositol 1,3,4,6-tetrakisphosphate 5-kinase, Inositol Polyphosphate Multikinase, IMPK) (FITC)
MBS6483449-02mL 0.2mL
EUR 980

IPMK (Inositol 1,3,4,6-tetrakisphosphate 5-kinase, Inositol Polyphosphate Multikinase, IMPK) (FITC)
MBS6483449-5x02mL 5x0.2mL
EUR 4250

IPMK (Inositol 1,3,4,6-tetrakisphosphate 5-kinase, Inositol Polyphosphate Multikinase, IMPK) (PE)
MBS6483456-02mL 0.2mL
EUR 980

IPMK (Inositol 1,3,4,6-tetrakisphosphate 5-kinase, Inositol Polyphosphate Multikinase, IMPK) (PE)
MBS6483456-5x02mL 5x0.2mL
EUR 4250

IP6K3, NT (IP6K3, IHPK3, Inositol hexakisphosphate kinase 3, Inositol hexaphosphate kinase 3)
MBS6010389-02mL 0.2(mL
EUR 695

IP6K3, NT (IP6K3, IHPK3, Inositol hexakisphosphate kinase 3, Inositol hexaphosphate kinase 3)
MBS6010389-5x02mL 5x0.2mL
EUR 2975

IPMK (Inositol Polyphosphate Multikinase, Inositol 1,3,4,6-tetrakisphosphate 5-kinase, IMPK) (Biotin)
MBS6483437-01mL 0.1mL
EUR 920

IPMK (Inositol Polyphosphate Multikinase, Inositol 1,3,4,6-tetrakisphosphate 5-kinase, IMPK) (Biotin)
MBS6483437-5x01mL 5x0.1mL
EUR 3990

IPMK (Inositol 1,3,4,6-tetrakisphosphate 5-kinase, Inositol Polyphosphate Multikinase, IMPK) (Biotin)
MBS6483448-02mL 0.2mL
EUR 980

IPMK (Inositol 1,3,4,6-tetrakisphosphate 5-kinase, Inositol Polyphosphate Multikinase, IMPK) (Biotin)
MBS6483448-5x02mL 5x0.2mL
EUR 4250

Screen Questâ„¢ Rhod-4 No Wash Calcium Assay Kit *Medium Removal*
36330-1Plate 1 Plate
EUR 109
Description: Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

Screen Questâ„¢ Rhod-4 No Wash Calcium Assay Kit *Medium Removal*
36331-10Plates 10 Plates
EUR 446
Description: Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

Screen Questâ„¢ Rhod-4 No Wash Calcium Assay Kit *Medium Removal*
36332-100Plates 100 Plates
EUR 3321
Description: Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

Antibiotic Medium No. 3 (U/P) (I/P) (Assay Broth, Penassay Broth)
66729 100 Gms
EUR 7.7
Description: Part D

Antibiotic Medium No. 3 (U/P) (I/P) (Assay Broth, Penassay Broth)
66729-1 500 Gms
EUR 25.66
Description: Part D

alpha-Glycerophosphoryl Inositol
TRC-G598770-100MG 100 mg Ask for price

IP6K3, NT (IP6K3, IHPK3, Inositol hexakisphosphate kinase 3, Inositol hexaphosphate kinase 3) (AP)
MBS6310696-02mL 0.2mL
EUR 980

IP6K3, NT (IP6K3, IHPK3, Inositol hexakisphosphate kinase 3, Inositol hexaphosphate kinase 3) (AP)
MBS6310696-5x02mL 5x0.2mL
EUR 4250

IP6K3, NT (IP6K3, IHPK3, Inositol hexakisphosphate kinase 3, Inositol hexaphosphate kinase 3) (APC)
MBS6310697-02mL 0.2mL
EUR 980

IP6K3, NT (IP6K3, IHPK3, Inositol hexakisphosphate kinase 3, Inositol hexaphosphate kinase 3) (APC)
MBS6310697-5x02mL 5x0.2mL
EUR 4250

IP6K3, NT (IP6K3, IHPK3, Inositol hexakisphosphate kinase 3, Inositol hexaphosphate kinase 3) (PE)
MBS6310706-02mL 0.2mL
EUR 980

IP6K3, NT (IP6K3, IHPK3, Inositol hexakisphosphate kinase 3, Inositol hexaphosphate kinase 3) (PE)
MBS6310706-5x02mL 5x0.2mL
EUR 4250

Inositol Triphosphate ELISA Kit
ECP7883 96 Tests
EUR 646

Inositol Triphosphate ELISA Kit
IMLIP3KT each
EUR 714
Description: Inositol Triphosphate ELISA Kit

IPMK (Inositol Polyphosphate Multikinase, Inositol 1,3,4,6-tetrakisphosphate 5-kinase, IMPK) (MaxLight 405)
MBS6483440-01mL 0.1mL
EUR 920

IPMK (Inositol Polyphosphate Multikinase, Inositol 1,3,4,6-tetrakisphosphate 5-kinase, IMPK) (MaxLight 405)
MBS6483440-5x01mL 5x0.1mL
EUR 3990

IPMK (Inositol Polyphosphate Multikinase, Inositol 1,3,4,6-tetrakisphosphate 5-kinase, IMPK) (MaxLight 490)
MBS6483441-01mL 0.1mL
EUR 920

 

Paraffin Wax
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Paraffin Wax Dispenser - EACH
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
Paraffin Wax White Soft pure
XH-90D Paraffin Wax Trimmer

Comparison of VEGF-A secretion from tumor cells under cellular stresses in conventional monolayer culture and microfluidic three-dimensional spheroid models

 

Vascular endothelial growth factor (VEGF) is a major cytokine in tumor biology affecting tumor survival, aggressiveness and pro-angiogenetic activities. In addition, cellular stresses often result in aggressive pro-angiogenetic behavior in tumors. For in vitro study, conventional monolayer cell culture has been broadly exploited; however, it often provides limited information due to its different microenvironment from that in vivo. Recently, three-dimensional (3D) cell spheroid culture provides in vivo-like microenvironments to study tumor biology and their survival mechanisms with better predictive power.
In this work, vascular endothelial growth factor of type A (VEGF-A) secretion from osteosarcoma (MG-63) cells cultured using monolayer and 3D spheroid models under two stress conditions: nutrient deficiency (reduced serum culture) and hypoxia-inducible factor (HIF) inhibition (HIF inhibitor, YC-1) are characterized and systematically compared.
In order to obtain ample sample size for consistent characterization of cellular responses from cancer spheroids under the stresses and compare the responses to those from the conventional monolayer model, a microfluidic spheroid formation and culture device is utilized in the experiments.
In the analysis, cell viability is estimated from captured images, and quantification of VEGF-A secreted from the cells is achieved using enzyme-linked immunosorbent assay (ELISA). The experimental results show that the viabilities decrease when the cells face higher stress levels in both monolayer and 3D spheroid culture models; however, the VEGF-A secretion profiles between the cell culture models are different.
The VEGF-A secretion decreases when the cells face higher stress conditions in the monolayer cell culture. In contrast, for the 3D spheroid culture, the VEGF-A concentration decreases for low stress levels but increases while the stress level is high. The VEGF-A regulation in the 3D models mimics in vivo cases of tumor survival and can provide insightful information to investigate tumor angiogenesis in vitro. The approach developed in this paper provides an efficient method to quantitatively and statistically study tumor growth kinetics and stress responses from highly uniform samples and it can also be applied to compare the underlying biomolecular mechanisms in monolayer and 3D spheroid culture models to elucidate the effects of microenvironments on cellular response in cancer research.

Protection of human induced pluripotent stem cells against shear stress in suspension culture by Bingham plastic fluid

 

Suspension culture is an important method used in the industrial preparation of pluripotent stem cells (PSCs), for regenerative therapy and drug screening. Generally, a suspension culture requires agitation to keep PSC aggregates suspended and to promote mass transfer, but agitation also causes cell damage. In this study, we investigated the use of a Bingham plastic fluid, supplemented with a polysaccharide-based polymer, to preserve PSCs from cell damage in suspension culture. Rheometric analysis showed that the culture medium gained yield stress and became a Bingham plastic fluid, after supplementation with the polymer FP003.

 

A growth/death analysis revealed that 2 days of aggregate formation and 2 days of suspension in the Bingham plastic medium improved cell growth and prevented cell death. After the initial aggregation step, whereas strong agitation (120 rpm) of a conventional culture medium resulted in massive cell death, in the Bingham plastic fluid we obtained the same growth as the normal culture with optimal agitation (90 rpm). This indicates that Bingham plastic fluid protected cells from shear stress in suspension culture and could be used to enhance their robustness when developing a large-scale. This article is protected by copyright. All rights reserved to Gentaur.

 

Metabolic profiling of CHO cell cultures at different working volumes and agitation speeds using spin tube reactors

 

Culture systems based on spin tube reactors have been consolidated in the development of manufacturing processes based on Chinese hamster ovary (CHO) cells. Despite their widespread use, there is little information about the consequences of varying operational setting parameters on the culture performance of recombinant CHO cell lines. Here we investigated the effect of varying working volumes and agitation speeds on cell growth, protein production and cell metabolism of two clonally-derived CHO cell lines (expressing an IgG1 and a ‘difficult-to-express’ fusion protein). Interestingly, low culture volumes increased recombinant protein production and decreased cell growth, while high culture volumes had the opposite effect. Altering agitation speeds exacerbated or moderated the differences observed due to culture volume changes, Gentaur.

 

Combining low agitation rates with high culture volumes suppressed growth and recombinant protein production in CHO cells. Meanwhile, high agitation rates narrowed the differences in culture performance between low and high working volumes. These differences were also reflected in cell metabolism, where low culture volumes enhanced oxidative metabolism (linked to a productive phenotype) and high culture volume generated a metabolic profile that was predominately glycolytic (linked to a proliferative phenotype). Our findings indicate that the culture volume influence on metabolism modulates the balance between cell growth and protein production, a key feature that may be useful to adjust CHO cells towards a more productive phenotype.

 

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red (Powder)
MBS653165-10L 10L
EUR 365

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red (Powder)
MBS653165-25L 25L
EUR 665

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red (Powder)
MBS653165-50L 50L
EUR 1285

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red (Powder)
MBS653165-5x50L 5x50L
EUR 5145

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red w/o Riboflavin (Powder)
MBS653037-10L 10L
EUR 365

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red w/o Riboflavin (Powder)
MBS653037-25L 25L
EUR 665

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red w/o Riboflavin (Powder)
MBS653037-50L 50L
EUR 1285

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red w/o Riboflavin (Powder)
MBS653037-5x50L 5x50L
EUR 5145

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red w/o Manganese (Powder)
MBS653209-10L 10L
EUR 365

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red w/o Manganese (Powder)
MBS653209-25L 25L
EUR 665

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red w/o Manganese (Powder)
MBS653209-50L 50L
EUR 1285

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red w/o Manganese (Powder)
MBS653209-5x50L 5x50L
EUR 5145

(±)-cistrans-Abscisic acid (synthetic)
A068-100MG 100 mg
EUR 108

(±)-cistrans-Abscisic acid (synthetic)
A068-500MG 500 mg
EUR 291.6

Polyclonal KRT5 / CK5 / Cytokeratin 5 Antibody (Synthetic peptide, corresponding to amino acids 550-600 of
APR17126G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human KRT5 / CK5 / Cytokeratin 5 (Synthetic peptide, corresponding to amino acids 550-600 of. This antibody is tested and proven to work in the following applications:

Yeast Nitrogen Base Medium without Amino Acids
HY-157347A Get quote Ask for price
Description: Yeast Nitrogen Base Medium without Amino Acids can be used in the cultivation of bacteria in genetic engineering[1].

(+/-)-Abscisic Acid Synthetic (ABA) for tissue culture, 98%
31240 25 Mg
EUR 12.6
Description: Part B

RPMI 1640 Medium w/o Amino Acids, Sodium Phosphate (Powder)
MBS653023-100L 100L
EUR 1405

RPMI 1640 Medium w/o Amino Acids, Sodium Phosphate (Powder)
MBS653023-10L 10L
EUR 310

RPMI 1640 Medium w/o Amino Acids, Sodium Phosphate (Powder)
MBS653023-25L 25L
EUR 570

RPMI 1640 Medium w/o Amino Acids, Sodium Phosphate (Powder)
MBS653023-50L 50L
EUR 930

RPMI 1640 Medium w/o Amino Acids, Sodium Phosphate (Powder)
MBS653023-5x100L 5x100L
EUR 5280

Basal Medium Eagle (BME) 100X Amino Acids, w/o Glutamine (Powder)
MBS653398-100L 100L
EUR 615

Basal Medium Eagle (BME) 100X Amino Acids, w/o Glutamine (Powder)
MBS653398-50L 50L
EUR 430

Basal Medium Eagle (BME) 100X Amino Acids, w/o Glutamine (Powder)
MBS653398-5x100L 5x100L
EUR 1710

Orcein, Synthetic
O03048 1G
EUR 141.71
Description: CAS N° 1400-62-0

PLGA poly(lactic-co-glycolic) acid); synthetic; (50% lactic and 50% glycolic acid, ~30-60 Kda)
AV-2010-1 0.1 g
EUR 226.8

PLGA poly(lactic-co-glycolic) acid); synthetic; (65% lactic and 35% glycolic acid, ~ 40-75 Kda)
AV-2020-1 1 g
EUR 226.8

PLGA poly(lactic-co-glycolic) acid); synthetic; (75% lactic and 25% glycolic acid, ~ 65-105 Kda)
AV-2030-1 1 g
EUR 270

Antipain (Synthetic)
MBS407511-100mg 100mg
EUR 435

Antipain (Synthetic)
MBS407511-25mg 25mg
EUR 225

Antipain (Synthetic)
MBS407511-5x100mg 5x100mg
EUR 1910

Antipain (Synthetic)
MBS407511-5x25mg 5x25mg
EUR 960

Williams' Medium E w/o L-Glutamine, w/o Amino Acids (Powder)
MBS652999-100L 100L
EUR 810

Williams' Medium E w/o L-Glutamine, w/o Amino Acids (Powder)
MBS652999-50L 50L
EUR 625

Williams' Medium E w/o L-Glutamine, w/o Amino Acids (Powder)
MBS652999-5x100L 5x100L
EUR 2600

Cerulenin (Synthetic)
C278590 100mg
EUR 19000
Description: 17397-89-6

Leupeptin (synthetic)
MBS566141-100mg 100mg
EUR 650

Leupeptin (synthetic)
MBS566141-25mg 25mg
EUR 250

Leupeptin (synthetic)
MBS566141-5mg 5mg
EUR 150

Leupeptin (synthetic)
MBS566141-5x100mg 5x100mg
EUR 2885

Leupeptin (Synthetic)
MBS407318-100mg 100mg
EUR 730

Leupeptin (Synthetic)
MBS407318-25mg 25mg
EUR 285

Leupeptin (Synthetic)
MBS407318-5x100mg 5x100mg
EUR 3250

Leupeptin (Synthetic)
MBS407318-5x25mg 5x25mg
EUR 1230

Leupeptin (Synthetic)
MBS407513-100mg 100mg
EUR 280

Leupeptin (Synthetic)
MBS407513-1g 1g
EUR 1490

Leupeptin (Synthetic)
MBS407513-25mg 25mg
EUR 175

Leupeptin (Synthetic)
MBS407513-5x100mg 5x100mg
EUR 1195

Leupeptin (Synthetic)
MBS407513-5x25mg 5x25mg
EUR 745

Cerulenin (Synthetic)
TRC-C278590-100MG 100 mg Ask for price

Mammary Epithelial Basal Medium MCDB 170 w/o Glucose, Amino Acids
PG106 4X 500ml bottle
EUR 0.1

Psychosine (Synthetic)
MBS393535-5mg 5mg
EUR 555

Psychosine (Synthetic)
MBS393535-5x5mg 5x5mg
EUR 2095

Lactacystin (Synthetic)
A2583-.1 100 µg
EUR 106.4
Description: Ubiquitination/ Proteasome|Proteasome

Lactacystin (Synthetic)
A2583-.5 500 µg
EUR 450.4
Description: Ubiquitination/ Proteasome|Proteasome

Lactacystin (Synthetic)
A2583-1 1 mg
EUR 786.4
Description: Ubiquitination/ Proteasome|Proteasome

Lactacystin (Synthetic)
1709-200 each
EUR 379.2

Resveratrol, synthetic
GP5884 100mg
EUR 115.8

Resveratrol, synthetic
GP5884-1 1
EUR 126.5

Resveratrol, synthetic
GP5884-100 100
EUR 28.4

Resveratrol, synthetic
GP5884-100MG 100 mg
EUR 70.8

Resveratrol, synthetic
GP5884-1G 1 g
EUR 189.6

Resveratrol, synthetic
GP5884-250 250
EUR 57

Resveratrol, synthetic
GP5884-250MG 250 mg
EUR 104.4

Swainsonine (Synthetic)
S885000 1mg
EUR 312
Description: 72741-87-8

Swainsonine (Synthetic)
TRC-S885000-5MG 5 mg Ask for price

Swainsonine (Synthetic)
TRC-S885000-25MG 25 mg Ask for price

Swainsonine (Synthetic)
TRC-S885000-1MG 1 mg Ask for price

Hydrotalcite, synthetic
H714835 100g
EUR 98
Description: 11097-59-9

Hydrotalcite, synthetic
TRC-H714835-100G 100 g Ask for price

Hydrotalcite, synthetic
TRC-H714835-50G 50 g Ask for price

Hydrotalcite, synthetic
TRC-H714835-10G 10 g Ask for price

Geranylgeraniol (Synthetic)
G367501 500mg
EUR 207
Description: 24034-73-9

Geranylgeraniol (Synthetic)
TRC-G367501-50MG 50 mg Ask for price

Geranylgeraniol (Synthetic)
TRC-G367501-500MG 500 mg Ask for price

Atpenin A5 (Synthetic)
2210-1000 each
EUR 548.4

Atpenin A5 (Synthetic)
2210-250 each
EUR 229.2

Synthetic Gastrin (GT)
RPU56912-100ug 100ug
EUR 470.4

Synthetic Gastrin (GT)
RPU56912-1mg 1mg
EUR 2184

Synthetic Gastrin (GT)
RPU56912-50ug 50ug
EUR 385

Synthetic BNP protein
MBS5308540-1mg 1mg
EUR 690

Synthetic BNP protein
MBS5308540-5x1mg 5x1mg
EUR 3070

Synthetic Apelin (APLN)
RPU57189-100ug 100ug
EUR 470.4

Synthetic Apelin (APLN)
RPU57189-1mg 1mg
EUR 2184

Synthetic Apelin (APLN)
RPU57189-50ug 50ug
EUR 385

BME 100X Amino Acids for Basal Medium Eagle (Modified). W/O L-glutamine.
BML02-500ML 500 ml
EUR 124.8
Description: 100X Amino Acids for Basal Medium Eagle (Modified). Without L-glutamine.

Williams' Medium E, w/o L-Glutamine, w/o Amino Acids, Glucose (Powder)
MBS6506786-100L 100L
EUR 810

Williams' Medium E, w/o L-Glutamine, w/o Amino Acids, Glucose (Powder)
MBS6506786-50L 50L
EUR 625

Williams' Medium E, w/o L-Glutamine, w/o Amino Acids, Glucose (Powder)
MBS6506786-5x100L 5x100L
EUR 2600

Menthol Synthetic pure
64370 100 Gms
EUR 6.5
Description: Part A

Pepstatin A (synthetic)
MBS566175-100mg 100mg
EUR 530

Pepstatin A (synthetic)
MBS566175-25mg 25mg
EUR 235

Pepstatin A (synthetic)
MBS566175-5mg 5mg
EUR 155

Pepstatin A (synthetic)
MBS566175-5x100mg 5x100mg
EUR 2315

Pepstatin A (Synthetic)
MBS407516-100mg 100mg
EUR 350

Pepstatin A (Synthetic)
MBS407516-1g 1g
EUR 2135

Pepstatin A (Synthetic)
MBS407516-25mg 25mg
EUR 200

Pepstatin A (Synthetic)
MBS407516-5x100mg 5x100mg
EUR 1525

Pepstatin A (Synthetic)
MBS407516-5x25mg 5x25mg
EUR 845

Epothilone B (synthetic)
E588990 10mg
EUR 117
Description: 152044-54-7

Epothilone D (synthetic)
E589000 10mg
EUR 552
Description: 189453-10-9

Synthetic Calcitonin (CT)
MBS2123983-001mg 0.01mg
EUR 155

Synthetic Calcitonin (CT)
MBS2123983-005mg 0.05mg
EUR 260

Synthetic Calcitonin (CT)
MBS2123983-01mg 0.1mg
EUR 375

Synthetic Calcitonin (CT)
MBS2123983-02mg 0.2mg
EUR 460

Synthetic Calcitonin (CT)
MBS2123983-05mg 0.5mg
EUR 860

Synthetic NEDD8 protein
MBS5308839-0025mg 0.025mg
EUR 460

Synthetic NEDD8 protein
MBS5308839-5x0025mg 5x0.025mg
EUR 2035

Synthetic Calcitonin (CT)
RPU56173-100ug 100ug
EUR 554.4

Synthetic Calcitonin (CT)
RPU56173-1mg 1mg
EUR 2184

Synthetic Calcitonin (CT)
RPU56173-50ug 50ug
EUR 336

Epothilone B (synthetic)
TRC-E588990-10MG 10 mg Ask for price

Epothilone B (synthetic)
TRC-E588990-5MG 5 mg Ask for price

Epothilone D (synthetic)
TRC-E589000-1MG 1 mg Ask for price

Epothilone D (synthetic)
TRC-E589000-25MG 25 mg Ask for price

Epothilone D (synthetic)
TRC-E589000-10MG 10 mg Ask for price

Balsam Canada Synthetic
102130 100 100 g Ask for price

Balsam Canada Synthetic
102130 500 500 g Ask for price

Synthetic Neurotensin (NT)
MBS2097730-001mg 0.01mg
EUR 140

Synthetic Neurotensin (NT)
MBS2097730-005mg 0.05mg
EUR 235

Synthetic Neurotensin (NT)
MBS2097730-01mg 0.1mg
EUR 325

Synthetic Neurotensin (NT)
MBS2097730-02mg 0.2mg
EUR 400

Synthetic Neurotensin (NT)
MBS2097730-05mg 0.5mg
EUR 745

Synthetic Antide protein
MBS5308514-1mg 1mg
EUR 480

Synthetic Antide protein
MBS5308514-5x1mg 5x1mg
EUR 2115

b-Sitosterol (Synthetic)
S497050 10mg
EUR 236
Description: 83-46-5

Synthetic Neurotensin (NT)
SPB203Hu01 10ug
EUR 120

Synthetic Neurotensin (NT)
4-SPB203Hu01
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 5 mg
  • 10ug
  • 50ug
  • 100 ug
  • 200 ug
  • 500 ug
  • 1 mg
Description: Recombinant Human Neurotensin expressed in: E.coli

Synthetic Neurotensin (NT)
RPU55424-100ug 100ug
EUR 369.6

Synthetic Neurotensin (NT)
RPU55424-1mg 1mg
EUR 1638

Synthetic Neurotensin (NT)
RPU55424-50ug 50ug
EUR 297

RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose (Powder)
MBS652918-100L 100L
EUR 1405

RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose (Powder)
MBS652918-10L 10L
EUR 310

RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose (Powder)
MBS652918-25L 25L
EUR 570

RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose (Powder)
MBS652918-50L 50L
EUR 930

RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose (Powder)
MBS652918-5x100L 5x100L
EUR 5280

SYNTHETIC HUMAN CEREBELLIN
GWB-7C45AE 0.1 mg Ask for price

Sphistin Synthetic Peptide
MBS5763362-1mg 1mg
EUR 235

Sphistin Synthetic Peptide
MBS5763362-5mg 5mg
EUR 520

Sphistin Synthetic Peptide
MBS5763362-5x5mg 5x5mg
EUR 2195

Synthetic Secretin protein
MBS5308933-1mg 1mg
EUR 455

Synthetic Secretin protein
MBS5308933-5x1mg 5x1mg
EUR 2005

Sphistin Synthetic Peptide
TP1036-10mg 10mg Ask for price
Description: Sphistin Synthetic Peptide

Sphistin Synthetic Peptide
TP1036-1g 1g Ask for price
Description: Sphistin Synthetic Peptide

Sphistin Synthetic Peptide
TP1036-1mg 1mg Ask for price
Description: Sphistin Synthetic Peptide

Sphistin Synthetic Peptide
TP1036-50mg 50mg Ask for price
Description: Sphistin Synthetic Peptide

Sphistin Synthetic Peptide
TP1036-5mg 5mg Ask for price
Description: Sphistin Synthetic Peptide

SYNTHETIC HUMAN OSTEOPONTIN
GWB-9D4DD0 0.05 mg Ask for price

Synthetic Elcatonin protein
MBS5308624-02mg 0.2mg
EUR 455

Synthetic Elcatonin protein
MBS5308624-5x02mg 5x0.2mg
EUR 2005

Synthetic Ubiquitin protein
MBS5309003-0025mg 0.025mg
EUR 460

Synthetic Ubiquitin protein
MBS5309003-5x0025mg 5x0.025mg
EUR 2035

Beta-Sitosterol (Synthetic)
TRC-S497050-10MG 10 mg Ask for price

Beta-Sitosterol (Synthetic)
TRC-S497050-25MG 25 mg Ask for price

Beta-Sitosterol (Synthetic)
TRC-S497050-1MG 1 mg Ask for price

Synthetic HIV-2 gp36
MBS142593-01mg 0.1mg
EUR 250

Synthetic HIV-2 gp36
MBS142593-05mg 0.5mg
EUR 455

Synthetic HIV-2 gp36
MBS142593-1mg 1mg
EUR 660

Synthetic HIV-2 gp36
MBS142593-5x1mg 5x1mg
EUR 2640

Synthetic HIV-2 gp36
rAP-5340 Inquiry Ask for price

Synthetic Leuprolide protein
MBS5308799-5mg 5mg
EUR 510
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