Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts

eaaci2015

Culture of Intestinal Epithelial Cell Monolayers and Their Use in Multiplex Macromolecular Permeability Assays for In Vitro Analysis of Tight Junction Size Selectivity

 

Tight junctions form a selectively permeable barrier that limits paracellular flux across epithelial-lined surfaces. Small molecules (less than ∼8 Å diameter) can traverse the junction via the size- and charge-selective, high-conductance pore pathway. In contrast, the low-conductance leak pathway accommodates larger macromolecules (up to ∼100 Å diameter) and is not charge-selective. Flux across the tight junction-independent, high-conductance, non-selective, unrestricted pathway occurs at sites of epithelial damage.

 

Cytokines can regulate each of these pathways, but commonly used measures of barrier function cannot discriminate between tight junction regulation and epithelial damage. This article describes methods for culturing intestinal epithelial cell monolayers and assessing the impact of cytokine treatment on leak and unrestricted pathway permeabilities. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Generation and culture of cell monolayers in Transwells Basic Protocol 2: Assessment of cytokine (IFNγ and TNF) treatment effects on barrier function Support Protocol: Immunofluorescent staining of monolayers Basic Protocol 3: Multiplex flux assay.

 

 

Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts

Introduction: Biomphalaria snails may display varying levels of susceptibility to Schistosoma mansoni infection. We have been developing an in vitro model to study the interaction between the snail and the parasite, using tissue-derived cell cultures from Biomphalaria.
Methods: The digestive gland- and kidney-derived cells from primary cultures of resistant (B. tenagophila Taim) and susceptible (B. tenagophila HM and B. glabrata BH) strains of Biomphalaria were exposed to S. mansoni sporocysts.
Results: S. mansoni sporocysts were surrounded and encapsulated exclusively by cells derived from the digestive gland (DG) of B. tenagophila Taim. The process was followed by a marked decrease in the number of free sporocysts in the culture medium. The morphological characteristics of DG-derived cells in culture have been described.
Conclusions: Cells derived from DG (but not SK) primary cultures of B. tenagophila Taim may participate in S. mansoni sporocyst control.
eaaci2015

eaaci2015

myo-Inositol

GC4902-1KG 1 kg
EUR 150

myo-Inositol

GC4902-250G 250 g
EUR 70

myo-Inositol

GC4902-25G 25 g
EUR 40

myo-Inositol

GC4902-500G 500 g
EUR 98

myo-Inositol

B7903-100 100 mg
EUR 108

myo-Inositol

B7903-500 500 mg
EUR 166

Myo-inositol

TBZ2449 20mg Ask for price

Screen Quest™ Rhod-4 No Wash Calcium Assay Kit *Medium Removal*

36330 1 Plate
EUR 132

Screen Quest™ Rhod-4 No Wash Calcium Assay Kit *Medium Removal*

36331 10 Plates
EUR 393

Screen Quest™ Rhod-4 No Wash Calcium Assay Kit *Medium Removal*

36332 100 Plates
EUR 2616

A Medium

DJ1018 100g
EUR 84.8

Advanced Medium

C0003-04 RT 500 mL Bottle
EUR 103

Medium 199

C0012-01 RT 500 mL Bottle
EUR 105

Heller's Medium

CP014-010 10X1L
EUR 99

Heller's Medium

CP014-500 50L
EUR 126

Hoagland's Medium

CP015-010 10X1L
EUR 99

Hoagland's Medium

CP015-500 50L
EUR 126

M9 Medium

SD7024 250g
EUR 71.75

M9CA Medium

SD7025 250g
EUR 71.75

YM Medium

SD7031 250g
EUR 70.45

TYGPN Medium

SD7032 250g
EUR 70.01

M63 Medium

SD7033 250g
EUR 71.75

NeuroProgenitor Medium

NM42400 125 ml
EUR 304

COLIFORM MEDIUM

C03-127-10kg 10 kg
EUR 1324

COLIFORM MEDIUM

C03-127-2kg 2kg
EUR 327

COLIFORM MEDIUM

C03-127-500g 500 g
EUR 125

HLP MEDIUM

H08-107-10kg 10 kg
EUR 2278

HLP MEDIUM

H08-107-2Kg 2 Kg
EUR 534

HLP MEDIUM

H08-107-500g 500 g
EUR 182

EC MEDIUM

E05-100-10kg 10 kg
EUR 814

EC MEDIUM

E05-100-2Kg 2 Kg
EUR 216

EC MEDIUM

E05-100-500g 500 g
EUR 95

DC MEDIUM

D04-117-10kg 10 kg
EUR 1579

DC MEDIUM

D04-117-2kg 2kg
EUR 382

DC MEDIUM

D04-117-500g 500 g
EUR 140

SIM MEDIUM

S19-110-10kg 10 kg
EUR 1021

SIM MEDIUM

S19-110-2kg 2kg
EUR 261

SIM MEDIUM

S19-110-500g 500 g
EUR 107

SOB MEDIUM

S19-124-10kg 10 kg
EUR 965

SOB MEDIUM

S19-124-2kg 2kg
EUR 249

SOB MEDIUM

S19-124-500g 500 g
EUR 104

D-chiro-Inositol

HY-N3021 10mg
EUR 108

D-myo-Inositol

IB0536 100g
EUR 67.4

1D-chiro-Inositol

GC3561-100MG 100 mg
EUR 102

1D-chiro-Inositol

GC3561-1G 1 g
EUR 269

1L-chiro-Inositol

GC7626-100MG 100 mg
EUR 102

1L-chiro-Inositol

GC7626-1G 1 g
EUR 269

1D-chiro-Inositol

TPC3089 20mg Ask for price

Insect Cell Medium: TNM-FH Insect Culture Medium

ABP-MED-10001 1 liter Ask for price

Insect Cell Medium: Serum-Free Insect Culture Medium

ABP-MED-10002 1 liter Ask for price

Insect Cell Medium: Grace?s Insect Medium (Unsupplemented)

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Inositol 2-Dehydrogenase/D-chiro-Inositol 3-Dehydrogenase (IOLG) Antibody

20-abx110510
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase Polyclonal Antibody

42631-100ul 100ul
EUR 333

Inositol Monophosphatase 1 (Recombinant)

20-abx073158
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Inositol Monophosphatase 2 (Recombinant)

20-abx073694
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg

Myo-Inositol Oxygenase (Recombinant)

20-abx073852
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
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  • 2 µg

L-alpha-Phosphatidyl inositol

GE1414-500MG 500 mg
EUR 126

H Medium Broth

DJ1019 100g
EUR 60.88

Aqueous Mounting Medium

AR1018 10mL X5 (Enough for 800-1200 assays)
EUR 80

Antifade Mounting Medium

AR1109 10mL (for 1000 assays)
EUR 65

AddexBio Cryopreservation Medium

C0002-20mL RT vial
EUR 104

RPMI 1640 Medium

abx098879-500ml 500 ml
EUR 175

Recovery Medium - 6x12mL

1711 4/PK
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Agarose, medium EEO

GE4750-100G 100 g
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Agarose, medium EEO

GE4750-500G 500 g
EUR 429

Schwann Growth Medium

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EUR 244

MCDB 131 Medium

CM034-050 500ml
EUR 85

MCDB 131 Medium

CM034-300 6x500ml
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MCDB 131 Medium

CM034-310 10x500ml
EUR 219

MCDB 131 Medium

CM034-320 20x500ml
EUR 340

MCDB 131 Medium

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Williams' Medium E

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Williams' Medium E

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Williams' Medium E

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Williams' Medium E

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Williams' Medium E

CM063-350 50x500ml
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Cereal Crops Medium

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Orchid Maintenance Medium

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Woody Plants Medium

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Woody Plants Medium

CP040-500 50L
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EverBrite Mounting Medium

23001 10mL
EUR 178
Description: Minimum order quantity: 1 unit of 10mL

Hepatocyte Maintenance Medium

HM42600 100 ml
EUR 409

Neuron Growth Medium

HNM001 500 ml
EUR 331

FluoreGuard Mounting Medium

FMM030 30 ml
EUR 96

FluoreGuard Mounting Medium

FMM060 60 ml
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FluoreGuard Mounting Medium

FMM500 500 ml
EUR 327

FluoreGuard Mounting Medium

FMM999 1000 ml
EUR 530

CARY & BLAIR MEDIUM

C03-131-10kg 10 kg
EUR 2185

CARY & BLAIR MEDIUM

C03-131-2Kg 2 Kg
EUR 514

CARY & BLAIR MEDIUM

C03-131-500g 500 g
EUR 176

 

 

Comparison of VEGF-A secretion from tumor cells under cellular stresses in conventional monolayer culture and microfluidic three-dimensional spheroid models

 

Vascular endothelial growth factor (VEGF) is a major cytokine in tumor biology affecting tumor survival, aggressiveness and pro-angiogenetic activities. In addition, cellular stresses often result in aggressive pro-angiogenetic behavior in tumors. For in vitro study, conventional monolayer cell culture has been broadly exploited; however, it often provides limited information due to its different microenvironment from that in vivo. Recently, three-dimensional (3D) cell spheroid culture provides in vivo-like microenvironments to study tumor biology and their survival mechanisms with better predictive power.
In this work, vascular endothelial growth factor of type A (VEGF-A) secretion from osteosarcoma (MG-63) cells cultured using monolayer and 3D spheroid models under two stress conditions: nutrient deficiency (reduced serum culture) and hypoxia-inducible factor (HIF) inhibition (HIF inhibitor, YC-1) are characterized and systematically compared.
In order to obtain ample sample size for consistent characterization of cellular responses from cancer spheroids under the stresses and compare the responses to those from the conventional monolayer model, a microfluidic spheroid formation and culture device is utilized in the experiments.
In the analysis, cell viability is estimated from captured images, and quantification of VEGF-A secreted from the cells is achieved using enzyme-linked immunosorbent assay (ELISA). The experimental results show that the viabilities decrease when the cells face higher stress levels in both monolayer and 3D spheroid culture models; however, the VEGF-A secretion profiles between the cell culture models are different.
The VEGF-A secretion decreases when the cells face higher stress conditions in the monolayer cell culture. In contrast, for the 3D spheroid culture, the VEGF-A concentration decreases for low stress levels but increases while the stress level is high. The VEGF-A regulation in the 3D models mimics in vivo cases of tumor survival and can provide insightful information to investigate tumor angiogenesis in vitro. The approach developed in this paper provides an efficient method to quantitatively and statistically study tumor growth kinetics and stress responses from highly uniform samples and it can also be applied to compare the underlying biomolecular mechanisms in monolayer and 3D spheroid culture models to elucidate the effects of microenvironments on cellular response in cancer research.

Protection of human induced pluripotent stem cells against shear stress in suspension culture by Bingham plastic fluid

 

Suspension culture is an important method used in the industrial preparation of pluripotent stem cells (PSCs), for regenerative therapy and drug screening. Generally, a suspension culture requires agitation to keep PSC aggregates suspended and to promote mass transfer, but agitation also causes cell damage. In this study, we investigated the use of a Bingham plastic fluid, supplemented with a polysaccharide-based polymer, to preserve PSCs from cell damage in suspension culture. Rheometric analysis showed that the culture medium gained yield stress and became a Bingham plastic fluid, after supplementation with the polymer FP003.

 

A growth/death analysis revealed that 2 days of aggregate formation and 2 days of suspension in the Bingham plastic medium improved cell growth and prevented cell death. After the initial aggregation step, whereas strong agitation (120 rpm) of a conventional culture medium resulted in massive cell death, in the Bingham plastic fluid we obtained the same growth as the normal culture with optimal agitation (90 rpm). This indicates that Bingham plastic fluid protected cells from shear stress in suspension culture and could be used to enhance their robustness when developing a large-scale. This article is protected by copyright. All rights reserved.

 

Metabolic profiling of CHO cell cultures at different working volumes and agitation speeds using spin tube reactors

 

Culture systems based on spin tube reactors have been consolidated in the development of manufacturing processes based on Chinese hamster ovary (CHO) cells. Despite their widespread use, there is little information about the consequences of varying operational setting parameters on the culture performance of recombinant CHO cell lines. Here we investigated the effect of varying working volumes and agitation speeds on cell growth, protein production and cell metabolism of two clonally-derived CHO cell lines (expressing an IgG1 and a ‘difficult-to-express’ fusion protein). Interestingly, low culture volumes increased recombinant protein production and decreased cell growth, while high culture volumes had the opposite effect. Altering agitation speeds exacerbated or moderated the differences observed due to culture volume changes.

 

Combining low agitation rates with high culture volumes suppressed growth and recombinant protein production in CHO cells. Meanwhile, high agitation rates narrowed the differences in culture performance between low and high working volumes. These differences were also reflected in cell metabolism, where low culture volumes enhanced oxidative metabolism (linked to a productive phenotype) and high culture volume generated a metabolic profile that was predominately glycolytic (linked to a proliferative phenotype). Our findings indicate that the culture volume influence on metabolism modulates the balance between cell growth and protein production, a key feature that may be useful to adjust CHO cells towards a more productive phenotype.

 

Resveratrol, synthetic

GP5884-250MG 250 mg
EUR 87

Lactacystin (Synthetic)

1709-200
EUR 316

5-Amino-4-oxopentanoic acid

HY-W000450 100mg
EUR 119

3-Amino-4-hydroxybenzoic acid

HY-W010224 100mg
EUR 108

3-Amino-4-methylpentanoic acid

HY-W012708 100mg
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3-Amino-2-methylpropanoic acid

HY-W012974 100mg
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β-Amino Acid Imagabalin Hydrochloride

HY-U00250 20mg
EUR 13439

D-Amino Acid Oxidase (Recombinant)

20-abx073765
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
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YNB W / O Amino acid

abx082010-100g 100 g
EUR 230

1-(Z-amino)Cyclohexanecarboxylic acid

5-02277 5g Ask for price

1-(Z-amino)Cyclohexanecarboxylic acid

5-02278 25g Ask for price

2-Amino-5-pyrazinecarboxylic acid

20-abx180281
  • EUR 996.00
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4-(Boc-Amino)Benzeneboronic Acid

20-abx180465
  • EUR 1135.00
  • EUR 300.00
  • EUR 467.00
  • 100 g
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  • 25 g

2-Amino-3,5-dichlorobenzoic acid

20-abx181010
  • EUR 467.00
  • EUR 300.00
  • EUR 217.00
  • 100 g
  • 25 g
  • 5 g

(PEO)4-amino-propionic acid

20-abx181441
  • EUR 606.00
  • EUR 356.00
  • EUR 1344.00
  • 1 g
  • 250 mg
  • 5 g

2-Amino-5-bromonicotinic acid

20-abx181694
  • EUR 857.00
  • EUR 300.00
  • 100 g
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2-Amino-5-Pyrimidineboronic Acid

20-abx181700
  • EUR 1316.00
  • EUR 509.00
  • EUR 244.00
  • 100 g
  • 25 g
  • 5 g

3-Amino-5-bromopicolinic acid

20-abx181890
  • EUR 940.00
  • EUR 634.00
  • EUR 2513.00
  • 1 g
  • 500 mg
  • 5 g

4-Amino-3-hydroxybenzoic acid

20-abx182035
  • EUR 258.00
  • EUR 189.00
  • 25 g
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2-Amino-2-phenylacetic acid

20-abx183045
  • EUR 217.00
  • EUR 314.00
  • 100 g
  • 500 g

2-Amino-4,5-dimethoxybenzoic acid

20-abx183054
  • EUR 258.00
  • EUR 203.00
  • 100 g
  • 25 g

2-Amino-4-methoxybenzoic acid

abx183059-100g 100 g
EUR 954

2-Amino-6-methoxybenzoic acid

20-abx183075
  • EUR 690.00
  • EUR 300.00
  • 100 g
  • 25 g

2-Amino-6-nitrobenzoic acid

20-abx183077
  • EUR 286.00
  • EUR 1233.00
  • EUR 523.00
  • 1 g
  • 25 g
  • 5 g

4-Amino-3-methoxybenzoic acid

20-abx185187
  • EUR 565.00
  • EUR 300.00
  • EUR 217.00
  • 100 g
  • 25 g
  • 5 g

2-Amino-4-Hydroxybenzoic Acid

20-abx188200
  • EUR 1748.00
  • EUR 509.00
  • EUR 1191.00
  • 10 g
  • 1 g
  • 5 g

2-Amino-5-Fluorobenzoic Acid

abx188201-100g 100 g
EUR 342

3-Amino-2-Methylbenzoic Acid

abx188349-1000g 1000 g
EUR 773

6-Amino-2-picolinic acid

20-abx183769
  • EUR 425.00
  • EUR 244.00
  • 100 g
  • 25 g

cis-3-Amino-cyclobutanecarboxylic acid

abx183945-1g 1 g
EUR 1219

3-Amino-4-methylbenzoic acid

20-abx184574
  • EUR 258.00
  • EUR 495.00
  • 100 g
  • 500 g

2-Amino-4-pyridinecarboxylic acid

20-abx185019
  • EUR 370.00
  • EUR 203.00
  • EUR 230.00
  • 100 g
  • 10 g
  • 25 g

3-Amino-5-bromobenzoic acid

GM0393-25G 25 g
EUR 190

11-(Boc-amino)-undecanoic acid

A-3310.0005 5.0g
EUR 151
Description: Sum Formula: C16H31NO4; CAS# [10436-25-6]

11-(Boc-amino)-undecanoic acid

A-3310.0025 25.0g
EUR 515
Description: Sum Formula: C16H31NO4; CAS# [10436-25-6]

2-Amino-5-chlorobenzoic acid

GM8338-10G 10 g
EUR 78

2-Amino-5-chlorobenzoic acid

GM8338-50G 50 g
EUR 134

1-(Fmoc-amino)-cyclopentanecarboxylic acid

B-3570.0001 1.0g
EUR 273
Description: Sum Formula: C21H21NO4; CAS# [117322-30-2]

1-(Fmoc-amino)-cyclopentanecarboxylic acid

B-3570.0005 5.0g
EUR 998
Description: Sum Formula: C21H21NO4; CAS# [117322-30-2]

Toolbox Fmoc-Amino Acid Derivatives

B-3840.0001 100.0g
EUR 624

anti-D Amino Acid Oxidase

YF-PA23561 50 ul
EUR 334
Description: Mouse polyclonal to D Amino Acid Oxidase

L Amino Acid Quantitation Kit

55R-1479 100 assays
EUR 654
Description: L Amino Acid Quantitation Kit for use in the research laboratory

Synthetic pyrethroid insecticides

AT123 1mg
EUR 1368

Synthetic pyrethroid insecticide

AG123 1 mg
EUR 523

Synthetic Triacylated Lipopeptide

VAdv-Ly0036 1 mg
EUR 1628
Description: Synthetic triacylated lipopeptide, a synthetic lipopeptide and a TLR2/1 agonist vaccine adjuvant.

Synthetic Neurotensin (NT)

4-SPB203Hu01
  • EUR 368.80
  • EUR 202.00
  • EUR 1108.00
  • EUR 436.00
  • EUR 772.00
  • EUR 310.00
  • EUR 2620.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Neurotensin expressed in: E.coli

Synthetic Phytohemagglutinin (PHA)

4-SPB359Ge01
  • EUR 368.80
  • EUR 202.00
  • EUR 1108.00
  • EUR 436.00
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  • EUR 310.00
  • EUR 2620.00
  • 100 ug
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  • 1 mg
  • 200 ug
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Description: Recombinant Pan-species Phytohemagglutinin expressed in: E.coli

Atpenin A5 (Synthetic)

2210-1000
EUR 457

Atpenin A5 (Synthetic)

2210-250
EUR 191

Fructosyl-amino acid oxidase from Microorganism

DIA-274 1ku
EUR 270

L-Amino Acid Assay Kit (Colorimetric)

MET-5054 100 assays
EUR 409

L-Amino Acid Assay Kit (Fluorometric)

MET-5055 100 assays
EUR 409

Branched Chain Amino Acid Assay Kit

MET-5056 192 assays
EUR 450

Native Porcine D-Amino Acid Oxidase

NATE-0180 400u
EUR 270

Fmoc-2-amino-5-Methoxybenzoic acid

5-04549 1g Ask for price

Fmoc-2-amino-5-Methoxybenzoic acid

5-04550 5g Ask for price

Recombinant Human D-Amino Acid Oxidase

7-02629 2µg Ask for price

Recombinant Human D-Amino Acid Oxidase

7-02630 10µg Ask for price

Recombinant Human D-Amino Acid Oxidase

7-02631 1mg Ask for price

D-Amino-Acid Oxidase (DAO) Antibody

abx431198-200ul 200 ul
EUR 286

D-Amino-Acid Oxidase (DAO) Antibody

abx432587-200ul 200 ul
EUR 384

D-Amino-Acid Oxidase (DAO) Antibody

abx432588-200ul 200 ul
EUR 384

5-Amino-2-chloro-isonicotinic acid

20-abx182161
  • EUR 1066.00
  • EUR 843.00
  • 25 g
  • 5 g

Boc-9-AMino-4,7-Dioxanonanoic acid

20-abx186634
  • EUR 370.00
  • EUR 495.00
  • 0.5 g
  • 1 g

Boc-15-Amino-4,7,10,13-Tetraoxapentadecanoic Acid

20-abx186635
  • EUR 439.00
  • EUR 578.00
  • 0.5 g
  • 1 g

Fmoc-12-Amino-4,7,10-Trioxadodecanoic Acid

abx186636-1g 1 g
EUR 370

2-((2-Aminoethyl)Amino)Acetic Acid

20-abx188143
  • EUR 1261.00
  • EUR 634.00
  • 25 g
  • 5 g

2-Amino-Isonicotinic Acid Ethyl Ester

20-abx188205
  • EUR 718.00
  • EUR 356.00
  • 100 g
  • 25 g

2-Hydroxy-4-Amino Butanoic Acid

abx188257-1kg 1 kg
EUR 746

Fmoc-21-Amino-4,7,10,13,16,19-Hexaoxaheneicosanoic Acid

20-abx184074
  • EUR 871.00
  • EUR 606.00
  • 1 g
  • 500 mg

Benzoic acid, o-amino-, ethyl ester

20-abx184649
  • EUR 286.00
  • EUR 217.00
  • EUR 578.00
  • 100 g
  • 25 g
  • 500 g

4-Amino-3-phenylbutyric acid HCl

20-abx183511
  • EUR 230.00
  • EUR 732.00
  • EUR 356.00
  • 1 g
  • 25 g
  • 5 g

(S)-(-)-2-Amino-4-pentenoic acid

GM0237-1G 1 g
EUR 90

(S)-(-)-2-Amino-4-pentenoic acid

GM0237-5G 5 g
EUR 237

Anti-D-amino-acid oxidase antibody

STJ71455 100 µg
EUR 359

Human D Amino Acid Oxidase Antibody

32963-05111 150 ug
EUR 261

Human D-amino-acid oxidase (DAO)

1-CSB-YP006494HU
  • EUR 430.00
  • EUR 234.00
  • EUR 1508.00
  • EUR 642.00
  • EUR 1009.00
  • EUR 291.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human D-amino-acid oxidase(DAO) expressed in Yeast

Human D-amino-acid oxidase (DAO)

1-CSB-EP006494HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human D-amino-acid oxidase(DAO) expressed in E.coli

Rat D-amino-acid oxidase (Dao)

1-CSB-EP006494RA
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Rat D-amino-acid oxidase(Dao) expressed in E.coli

Recombinant rat D-amino-acid oxidase

P1766 100ug Ask for price
Description: Recombinant protein for rat D-amino-acid oxidase

4-Amino-piperidine-4-carboxylic acid

F-3605.0001 1.0g
EUR 345
Description: Sum Formula: C6H12N2O2; CAS# [40951-39-1] net

4-Amino-piperidine-4-carboxylic acid

F-3605.0005 5.0g
EUR 1301
Description: Sum Formula: C6H12N2O2; CAS# [40951-39-1] net

4-Amino-piperidine-4-carboxylic acid

F-3605.0025 25.0g
EUR 5108
Description: Sum Formula: C6H12N2O2; CAS# [40951-39-1] net

Human Amino Acid Metabolism Primer Library

HAMM-I 1 set
EUR 645

D-amino acid oxidase, human recombinant

P1052-10
EUR 164

D-amino acid oxidase, human recombinant

P1052-50
EUR 588

Anti-D Amino Acid Oxidase (2F5)

YF-MA12633 100 ug
EUR 363
Description: Mouse monoclonal to D Amino Acid Oxidase

Branched Chain Amino Acid Assay Kit

55R-1440 100 assays
EUR 740
Description: Assay Kit for detection of Branched Chain Amino Acids in the research laboratory

D-Amino-Acid Oxidase Recombinant Protein

92-027 0.05 mg
EUR 710.75
Description: D-Amino-Acid Oxidase (DAO) belongs to the DAMOX/DASOX family. DAO is a peroxisomal enzyme which founctions as a homodimer to oxidizes D-amino acids to the corresponding imino acids, producing ammonia and hydrogen peroxide. D-amino-acid oxidase regulates the level of the neuromodulator D-serine in the brain, has a high activity towards D-DOPA and contributes to dopamine synthesis. D-amino-acid oxidase could act as a detoxifying agent which removes D-amino acids accumulated during aging. It also acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups.

D-Amino Acid Oxidase/ DAMOX (DAO) Antibody

20-abx301170
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Aromatic-L-Amino-Acid Decarboxylase (DDC) Antibody

20-abx302246
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Native Crotalus adamanteus L-Amino Acid Oxidase

NATE-0366 2 mg
EUR 270