Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts

November 12, 2020
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eaaci2015

Culture of Intestinal Epithelial Cell Monolayers and Their Use in Multiplex Macromolecular Permeability Assays for In Vitro Analysis of Tight Junction Size Selectivity

 

Tight junctions form a selectively permeable barrier that limits paracellular flux across epithelial-lined surfaces. Small molecules (less than ∼8 Å diameter) can traverse the junction via the size- and charge-selective, high-conductance pore pathway. In contrast, the low-conductance leak pathway accommodates larger macromolecules (up to ∼100 Å diameter) and is not charge-selective. Flux across the tight junction-independent, high-conductance, non-selective, unrestricted pathway occurs at sites of epithelial damage.

 

Cytokines can regulate each of these pathways, but commonly used measures of barrier function cannot discriminate between tight junction regulation and epithelial damage. This article describes methods for culturing intestinal epithelial cell monolayers and assessing the impact of cytokine treatment on leak and unrestricted pathway permeabilities. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Generation and culture of cell monolayers in Transwells Basic Protocol 2: Assessment of cytokine (IFNγ and TNF) treatment effects on barrier function Support Protocol: Immunofluorescent staining of monolayers Basic Protocol 3: Multiplex flux assay.

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Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts

Introduction: Biomphalaria snails may display varying levels of susceptibility to Schistosoma mansoni infection. We have been developing an in vitro model to study the interaction between the snail and the parasite, using tissue-derived cell cultures from Biomphalaria.
Methods: The digestive gland- and kidney-derived cells from primary cultures of resistant (B. tenagophila Taim) and susceptible (B. tenagophila HM and B. glabrata BH) strains of Biomphalaria were exposed to S. mansoni sporocysts.
Results: S. mansoni sporocysts were surrounded and encapsulated exclusively by cells derived from the digestive gland (DG) of B. tenagophila Taim. The process was followed by a marked decrease in the number of free sporocysts in the culture medium. The morphological characteristics of DG-derived cells in culture have been described.
Conclusions: Cells derived from DG (but not SK) primary cultures of B. tenagophila Taim may participate in S. mansoni sporocyst control.
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eaaci2015

RPMI 1640 Medium w/o L-Glutamine, Inositol (Powder)
MBS652997-25L 25L
EUR 540

RPMI 1640 Medium w/o L-Glutamine, Inositol (Powder)
MBS652997-50L 50L
EUR 860

RPMI 1640 Medium w/o L-Glutamine, Inositol (Powder)
MBS652997-5x100L 5x100L
EUR 6570

Inositol Triphosphate (IP3) Magnetic Luminex Assay Kit
LKU603314-96T 96T
EUR 1125.9

Myo Inositol Oxygenase (MIOX) Magnetic Luminex Assay Kit
LKU605077-96T 96T
EUR 966

Assay Medium 2C
78544-1 100 ml
EUR 120
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Assay Medium 2C
78544-2 500 ml
EUR 600
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Assay Medium 7A
78673-1 100 ml
EUR 120
Description: This liquid Assay Medium is optimized for use with BPS Bioscience cell-based assay  

Assay Medium 7A
78673-2 500 ml
EUR 600
Description: This liquid Assay Medium is optimized for use with BPS Bioscience cell-based assay  

Assay Medium 1C
78674-1 100 ml
EUR 120
Description: This liquid Assay Medium is optimized for use with BPS Bioscience cell-based assay.

Assay Medium 1C
78674-2 500 ml
EUR 600
Description: This liquid Assay Medium is optimized for use with BPS Bioscience cell-based assay.

Assay Medium 1B
79617-1 100 ml
EUR 135
Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• TGF/SMAD Signaling Pathway SBE Reporter - HEK293 Cell Line #60653 _x000D_• AP-1 Reporter - HEK293 Recombinant Cell Line (JNK signaling pathway) #60405

Assay Medium 1B
79617-2 500 ml
EUR 675
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• TGF/SMAD Signaling Pathway SBE Reporter - HEK293 Cell Line #60653 _x000D_• AP-1 Reporter - HEK293 Recombinant Cell Line (JNK signaling pathway) #60405

Assay Medium 2B
79619-1 100 ml
EUR 120
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• Foxp3 Reporter (Luc) - Jurkat Recombinant Cell Line -#60628

Assay Medium 2B
79619-2 500 ml
EUR 600
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• Foxp3 Reporter (Luc) - Jurkat Recombinant Cell Line -#60628

Assay Medium 5A
79620-1 100 ml
EUR 120
Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience cell lines.

Assay Medium 5A
79620-2 500 ml
EUR 600
Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience cell lines.

Assay Medium 2A
79621-1 100 ml
EUR 120
Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• ADCC Bioassay Effector Cell F variant (Low Affinity) - Jurkat Recombinant Cell Line #60540 _x000D_• ADCC Bioassay Effector Cell V variant (High Affinity) - Jurkat Recombinant Cell Line #60541

Assay Medium 2A
79621-2 500 ml
EUR 600
Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• ADCC Bioassay Effector Cell F variant (Low Affinity) - Jurkat Recombinant Cell Line #60540 _x000D_• ADCC Bioassay Effector Cell V variant (High Affinity) - Jurkat Recombinant Cell Line #60541

Assay Medium 2C
79623-1 100 ml
EUR 120
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• TIGIT / NFAT Reporter - Jurkat Recombinant Cell Line - #60538_x000D_• CD155/TCR Activator - CHO Recombinant Cell Line -#60548

Assay Medium 2C
79623-2 500 ml
EUR 600
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• TIGIT / NFAT Reporter - Jurkat Recombinant Cell Line, #60538 • CD155/TCR Activator - CHO Recombinant Cell Line, #60548

Assay Medium 7B
79718-1 100 ml
EUR 120
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience cell lines._x000D_Optimized for use with Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway), #60520

Assay Medium 7B
79718-2 500 ml
EUR 600
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience cell lines._x000D_Optimized for use with Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway), #60520

Assay Medium 1A
79805-1 100 ml
EUR 120
Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including TrkA / SRE Reporter - HEK293 Recombinant Cell Line (#79798)

Assay Medium 1A
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EUR 600
Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including TrkA / SRE Reporter - HEK293 Recombinant Cell Line (#79798)

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EUR 117.94
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EUR 36.55
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10666 100 Gms
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Antibiotic Assay Medium No.3 (Assay
MU042-500G 1 unit
EUR 41.3
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79816-1 100 ml
EUR 120
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Assay Medium 3A -500 ml
79816-2 500 ml
EUR 600
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*Folic Acid Assay Medium
M038-100G 1 unit
EUR 31.84
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*Folic Acid Assay Medium
M038-500G 1 unit
EUR 129.77
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EUR 1062.6

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Antibiotic Assay Medium G
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EUR 46.46
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Antibiotic Assay Medium G
M553B-500G 1 unit
EUR 48.25
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Antibiotic Assay Medium C
M555-500G 1 unit
EUR 44.54
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EUR 48.84
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M556-500G 1 unit
EUR 44.54
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Antibiotic Assay Medium D
M556B-500G 1 unit
EUR 48.17
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Antibiotic Assay Medium I
M1847B-500G 1 unit
EUR 47.77
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Antibiotic Assay Medium H
M1863B-500G 1 unit
EUR 44.21
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Antibiotic Assay Medium F
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EUR 46.46
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Antibiotic Assay Medium F
M923B-500G 1 unit
EUR 48.25
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Antibiotic Assay Medium H
M1665-500G 1 unit
EUR 46.46
Description: Antibiotic Assay Medium H

Antibiotic Assay Medium A
M003B-100G 1 unit
EUR 17.21
Description: Antibiotic Assay Medium A

Antibiotic Assay Medium A
M003B-500G 1 unit
EUR 43.27
Description: Antibiotic Assay Medium A

Antibiotic Assay Medium A
M004B-100G 1 unit
EUR 16.77
Description: Antibiotic Assay Medium A

Antibiotic Assay Medium A
M004B-500G 1 unit
EUR 43.27
Description: Antibiotic Assay Medium A

Antibiotic Assay Medium B
M1346-500G 1 unit
EUR 46.8
Description: Antibiotic Assay Medium B

Antibiotic Assay Medium B
M1346B-500G 1 unit
EUR 49.05
Description: Antibiotic Assay Medium B

Antibiotic Assay Medium E
M1347-500G 1 unit
EUR 44.78
Description: Antibiotic Assay Medium E

Antibiotic Assay Medium E
M1347B-500G 1 unit
EUR 46.46
Description: Antibiotic Assay Medium E

Antibiotic Assay Medium A
MM003-100G 1 unit
EUR 17.21
Description: Antibiotic Assay Medium A

Antibiotic Assay Medium A
MM003-500G 1 unit
EUR 43.27
Description: Antibiotic Assay Medium A

Antibiotic Assay Medium D
MM004-100G 1 unit
EUR 15.93
Description: Antibiotic Assay Medium D

Antibiotic Assay Medium D
MM004-500G 1 unit
EUR 41.77
Description: Antibiotic Assay Medium D

Antibiotic Assay Medium B
MM005-500G 1 unit
EUR 49.24
Description: Antibiotic Assay Medium B

Antibiotic Assay Medium E
MM006-500G 1 unit
EUR 49.15
Description: Antibiotic Assay Medium E

Antibiotic Assay Medium F
MM041-500G 1 unit
EUR 46.9
Description: Antibiotic Assay Medium F

Antibiotic Assay Medium C
MM042-100G 1 unit
EUR 14.77
Description: Antibiotic Assay Medium C

Antibiotic Assay Medium C
MM042-500G 1 unit
EUR 41.3
Description: Antibiotic Assay Medium C

Antibiotic Assay Medium G
MM101-500G 1 unit
EUR 46.46
Description: Antibiotic Assay Medium G

Antibiotic Assay Medium J
MM1666-100G 1 unit
EUR 15.93
Description: Antibiotic Assay Medium J

Antibiotic Assay Medium J
MM1666-500G 1 unit
EUR 37.94
Description: Antibiotic Assay Medium J

Antibiotic Assay Medium H
MM225-500G 1 unit
EUR 49.15
Description: Antibiotic Assay Medium H

Antibiotic Assay Medium I
MM798-500G 1 unit
EUR 46.46
Description: Antibiotic Assay Medium I

Antibiotic Assay Medium A
ME003-100G 1 unit
EUR 17.21
Description: Antibiotic Assay Medium A

Antibiotic Assay Medium A
ME003-500G 1 unit
EUR 43.27
Description: Antibiotic Assay Medium A

Antibiotic Assay Medium A
ME004-100G 1 unit
EUR 14.54
Description: Antibiotic Assay Medium A

Antibiotic Assay Medium A
ME004-500G 1 unit
EUR 43.27
Description: Antibiotic Assay Medium A

Antibiotic Assay Medium B
ME1346-500G 1 unit
EUR 49.05
Description: Antibiotic Assay Medium B

Antibiotic Assay Medium E
ME1347-500G 1 unit
EUR 46.46
Description: Antibiotic Assay Medium E

Antibiotic Assay Medium H
ME1665-500G 1 unit
EUR 46.46
Description: Antibiotic Assay Medium H

Antibiotic Assay Medium G
ME553-500G 1 unit
EUR 48.25
Description: Antibiotic Assay Medium G

Antibiotic Assay Medium C
ME555-500G 1 unit
EUR 48.17
Description: Antibiotic Assay Medium C

Antibiotic Assay Medium D
ME556-500G 1 unit
EUR 48.17
Description: Antibiotic Assay Medium D

Antibiotic Assay Medium F
ME923-500G 1 unit
EUR 48.25
Description: Antibiotic Assay Medium F

*Pantothenate Assay Medium, AOAC
M1281-100G 1 unit
EUR 33.1
Description: *Pantothenate Assay Medium, AOAC

Inositol Polyphosphate-4-Phosphatase Type II 105kDa (INPP4B) Magnetic Luminex Assay Kit
LKU600468-96T 96T
EUR 1062.6

Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) Magnetic Luminex Assay Kit
LKU600474-96T 96T
EUR 1062.6

Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) Magnetic Luminex Assay Kit
LKU600478-96T 96T
EUR 1092.5

Antibiotic Assay Medium No.6
M223-500G 1 unit
EUR 42.44
Description: Antibiotic Assay Medium No.6

Antibiotic Assay Medium No.34
M797-500G 1 unit
EUR 42.44
Description: Antibiotic Assay Medium No.34

Antibiotic Assay Medium No.35
M798-500G 1 unit
EUR 44.78
Description: Antibiotic Assay Medium No.35

Antibiotic Assay Medium No.38
M799-500G 1 unit
EUR 44.78
Description: Antibiotic Assay Medium No.38

Antibiotic Assay Medium No.36
M1666-100G 1 unit
EUR 14.77
Description: Antibiotic Assay Medium No.36

Antibiotic Assay Medium No.36
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EUR 34.73
Description: Antibiotic Assay Medium No.36

Antibiotic Assay Medium No.37
M1667-100G 1 unit
EUR 9.83
Description: Antibiotic Assay Medium No.37

Antibiotic Assay Medium No.37
M1667-500G 1 unit
EUR 16.87
Description: Antibiotic Assay Medium No.37

Antibiotic Assay Medium No. 20
M167-500G 1 unit
EUR 45.2
Description: Antibiotic Assay Medium No. 20

Antibiotic Assay Medium No.3
M042-100G 1 unit
EUR 14.77
Description: Antibiotic Assay Medium No.3

Antibiotic Assay Medium No.3
M042-500G 1 unit
EUR 38.13
Description: Antibiotic Assay Medium No.3

Antibiotic Assay Medium No.19
M101-500G 1 unit
EUR 44.78
Description: Antibiotic Assay Medium No.19

Antibiotic Assay Medium No.32
M1141-500G 1 unit
EUR 44.78
Description: Antibiotic Assay Medium No.32

Antibiotic Assay Medium No.39
M1142-500G 1 unit
EUR 42.44
Description: Antibiotic Assay Medium No.39

Antibiotic Assay Medium No.40
M1143-500G 1 unit
EUR 44.78
Description: Antibiotic Assay Medium No.40

Antibiotic Assay Medium No.41
M1144-500G 1 unit
EUR 42.44
Description: Antibiotic Assay Medium No.41

Antibiotic Assay Medium No. 4
M140-500G 1 unit
EUR 47.77
Description: Antibiotic Assay Medium No. 4

Antibiotic Assay Medium No.11
MU004-100G 1 unit
EUR 15.93
Description: Antibiotic Assay Medium No.11

Antibiotic Assay Medium No.11
MU004-500G 1 unit
EUR 41.77
Description: Antibiotic Assay Medium No.11

Antibiotic Assay Medium No.5
MU006-500G 1 unit
EUR 49.24
Description: Antibiotic Assay Medium No.5

Antibiotic Assay Medium No. 8
MU041-500G 1 unit
EUR 46.9
Description: Antibiotic Assay Medium No. 8

Antibiotic Assay Medium No.19
MU101-500G 1 unit
EUR 46.46
Description: Antibiotic Assay Medium No.19

Antibiotic Assay Medium No.32
MU1141-500G 1 unit
EUR 46.46
Description: Antibiotic Assay Medium No.32

Antibiotic Assay Medium No.39
MU1142-500G 1 unit
EUR 46.46
Description: Antibiotic Assay Medium No.39

Antibiotic Assay Medium No.40
MU1143-500G 1 unit
EUR 46.46
Description: Antibiotic Assay Medium No.40

Antibiotic Assay Medium No. 41
MU1144-500G 1 unit
EUR 46.46
Description: Antibiotic Assay Medium No. 41

Antibiotic Assay Medium No. 36
MU1666-100G 1 unit
EUR 15.93
Description: Antibiotic Assay Medium No. 36

Antibiotic Assay Medium No. 36
MU1666-500G 1 unit
EUR 37.94
Description: Antibiotic Assay Medium No. 36

Antibiotic Assay Medium No.37
MU1667-100G 1 unit
EUR 9.83
Description: Antibiotic Assay Medium No.37

Antibiotic Assay Medium No.37
MU1667-500G 1 unit
EUR 18.6
Description: Antibiotic Assay Medium No.37

Antibiotic Assay Medium No.10
MU225-500G 1 unit
EUR 49.15
Description: Antibiotic Assay Medium No.10

Antibiotic Assay Medium No.13
MU254-500G 1 unit
EUR 46.46
Description: Antibiotic Assay Medium No.13

Antibiotic Assay Medium No.34
MU797-500G 1 unit
EUR 46.46
Description: Antibiotic Assay Medium No.34

Antibiotic Assay Medium No.35
MU798-500G 1 unit
EUR 46.46
Description: Antibiotic Assay Medium No.35

Antibiotic Assay Medium No.38
MU799-500G 1 unit
EUR 46.46
Description: Antibiotic Assay Medium No.38

Antibiotic Assay Medium L- AOAC
M991-500G 1 unit
EUR 46.46
Description: Antibiotic Assay Medium L- AOAC

Antibiotic Assay Medium M- AOAC
M992-500G 1 unit
EUR 46.46
Description: Antibiotic Assay Medium M- AOAC

PD-1 Cell-Based Assay Medium
60189 100 ml
EUR 120
Description: This liquid Assay Medium is optimized for use with the PD-1 cell-based assay using the BPS Bioscience's PD-1/NFAT Reporter-Jurkat cell line (Cat #60535). _x000D_

Antibiotic Assay Medium No.4 (Yeast
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EUR 49.58
Description: Antibiotic Assay Medium No.4 (Yeast

Antibiotic HiVeg Assay Medium No.1
MV003-100G 1 unit
EUR 15.93
Description: Antibiotic HiVeg Assay Medium No.1

Antibiotic HiVeg Assay Medium No.1
MV003-500G 1 unit
EUR 39.66
Description: Antibiotic HiVeg Assay Medium No.1

Antibiotic HiVeg Assay Medium No.11
MV004-100G 1 unit
EUR 15.93
Description: Antibiotic HiVeg Assay Medium No.11

Antibiotic HiVeg Assay Medium No.11
MV004-500G 1 unit
EUR 39.66
Description: Antibiotic HiVeg Assay Medium No.11

Antibiotic HiVeg Assay Medium No.2
MV005-500G 1 unit
EUR 45.01
Description: Antibiotic HiVeg Assay Medium No.2

Antibiotic HiVeg Assay Medium No.5
MV006-500G 1 unit
EUR 47.01
Description: Antibiotic HiVeg Assay Medium No.5

Antibiotic HiVeg Assay Medium No. 8
MV041-500G 1 unit
EUR 42.94
Description: Antibiotic HiVeg Assay Medium No. 8

Antibiotic HiVeg Assay Medium No.3
MV042-100G 1 unit
EUR 14.77
Description: Antibiotic HiVeg Assay Medium No.3

Antibiotic HiVeg Assay Medium No.3
MV042-500G 1 unit
EUR 38.13
Description: Antibiotic HiVeg Assay Medium No.3

Antibiotic HiVeg Assay Medium No.19
MV101-500G 1 unit
EUR 44.78
Description: Antibiotic HiVeg Assay Medium No.19

Antibiotic HiVeg Assay Medium No.32
MV1141-500G 1 unit
EUR 44.78
Description: Antibiotic HiVeg Assay Medium No.32

Antibiotic HiVeg Assay Medium No.39
MV1142-500G 1 unit
EUR 42.44
Description: Antibiotic HiVeg Assay Medium No.39

Antibiotic HiVeg Assay Medium No.40
MV1143-500G 1 unit
EUR 44.78
Description: Antibiotic HiVeg Assay Medium No.40

Antibiotic HiVeg Assay Medium No.41
MV1144-500G 1 unit
EUR 42.44
Description: Antibiotic HiVeg Assay Medium No.41

Antibiotic HiVeg Assay Medium No. 4
MV140-500G 1 unit
EUR 47.77
Description: Antibiotic HiVeg Assay Medium No. 4

Antibiotic HiVeg Assay Medium No. 9
MV147-500G 1 unit
EUR 44.78
Description: Antibiotic HiVeg Assay Medium No. 9

Antibiotic HiVeg Assay Medium No.36
MV1666-100G 1 unit
EUR 14.77
Description: Antibiotic HiVeg Assay Medium No.36

Antibiotic HiVeg Assay Medium No.36
MV1666-500G 1 unit
EUR 34.73
Description: Antibiotic HiVeg Assay Medium No.36

Antibiotic HiVeg Assay Medium No.37
MV1667-100G 1 unit
EUR 9.83
Description: Antibiotic HiVeg Assay Medium No.37

Antibiotic HiVeg Assay Medium No.37
MV1667-500G 1 unit
EUR 16.87
Description: Antibiotic HiVeg Assay Medium No.37

Antibiotic HiVeg Assay Medium No.6
MV223-500G 1 unit
EUR 42.44
Description: Antibiotic HiVeg Assay Medium No.6

Antibiotic HiVeg Assay Medium No.10
MV225-500G 1 unit
EUR 44.78
Description: Antibiotic HiVeg Assay Medium No.10

Antibiotic HiVeg Assay Medium No.13
MV254-500G 1 unit
EUR 44.21
Description: Antibiotic HiVeg Assay Medium No.13

Antibiotic HiVeg Assay Medium No.12
MV280-500G 1 unit
EUR 46.9
Description: Antibiotic HiVeg Assay Medium No.12

 

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Comparison of VEGF-A secretion from tumor cells under cellular stresses in conventional monolayer culture and microfluidic three-dimensional spheroid models

 

Vascular endothelial growth factor (VEGF) is a major cytokine in tumor biology affecting tumor survival, aggressiveness and pro-angiogenetic activities. In addition, cellular stresses often result in aggressive pro-angiogenetic behavior in tumors. For in vitro study, conventional monolayer cell culture has been broadly exploited; however, it often provides limited information due to its different microenvironment from that in vivo. Recently, three-dimensional (3D) cell spheroid culture provides in vivo-like microenvironments to study tumor biology and their survival mechanisms with better predictive power.
In this work, vascular endothelial growth factor of type A (VEGF-A) secretion from osteosarcoma (MG-63) cells cultured using monolayer and 3D spheroid models under two stress conditions: nutrient deficiency (reduced serum culture) and hypoxia-inducible factor (HIF) inhibition (HIF inhibitor, YC-1) are characterized and systematically compared.
In order to obtain ample sample size for consistent characterization of cellular responses from cancer spheroids under the stresses and compare the responses to those from the conventional monolayer model, a microfluidic spheroid formation and culture device is utilized in the experiments.
In the analysis, cell viability is estimated from captured images, and quantification of VEGF-A secreted from the cells is achieved using enzyme-linked immunosorbent assay (ELISA). The experimental results show that the viabilities decrease when the cells face higher stress levels in both monolayer and 3D spheroid culture models; however, the VEGF-A secretion profiles between the cell culture models are different.
The VEGF-A secretion decreases when the cells face higher stress conditions in the monolayer cell culture. In contrast, for the 3D spheroid culture, the VEGF-A concentration decreases for low stress levels but increases while the stress level is high. The VEGF-A regulation in the 3D models mimics in vivo cases of tumor survival and can provide insightful information to investigate tumor angiogenesis in vitro. The approach developed in this paper provides an efficient method to quantitatively and statistically study tumor growth kinetics and stress responses from highly uniform samples and it can also be applied to compare the underlying biomolecular mechanisms in monolayer and 3D spheroid culture models to elucidate the effects of microenvironments on cellular response in cancer research.

Protection of human induced pluripotent stem cells against shear stress in suspension culture by Bingham plastic fluid

 

Suspension culture is an important method used in the industrial preparation of pluripotent stem cells (PSCs), for regenerative therapy and drug screening. Generally, a suspension culture requires agitation to keep PSC aggregates suspended and to promote mass transfer, but agitation also causes cell damage. In this study, we investigated the use of a Bingham plastic fluid, supplemented with a polysaccharide-based polymer, to preserve PSCs from cell damage in suspension culture. Rheometric analysis showed that the culture medium gained yield stress and became a Bingham plastic fluid, after supplementation with the polymer FP003.

 

A growth/death analysis revealed that 2 days of aggregate formation and 2 days of suspension in the Bingham plastic medium improved cell growth and prevented cell death. After the initial aggregation step, whereas strong agitation (120 rpm) of a conventional culture medium resulted in massive cell death, in the Bingham plastic fluid we obtained the same growth as the normal culture with optimal agitation (90 rpm). This indicates that Bingham plastic fluid protected cells from shear stress in suspension culture and could be used to enhance their robustness when developing a large-scale. This article is protected by copyright. All rights reserved to Gentaur.

 

Metabolic profiling of CHO cell cultures at different working volumes and agitation speeds using spin tube reactors

 

Culture systems based on spin tube reactors have been consolidated in the development of manufacturing processes based on Chinese hamster ovary (CHO) cells. Despite their widespread use, there is little information about the consequences of varying operational setting parameters on the culture performance of recombinant CHO cell lines. Here we investigated the effect of varying working volumes and agitation speeds on cell growth, protein production and cell metabolism of two clonally-derived CHO cell lines (expressing an IgG1 and a ‘difficult-to-express’ fusion protein). Interestingly, low culture volumes increased recombinant protein production and decreased cell growth, while high culture volumes had the opposite effect. Altering agitation speeds exacerbated or moderated the differences observed due to culture volume changes, Gentaur.

 

Combining low agitation rates with high culture volumes suppressed growth and recombinant protein production in CHO cells. Meanwhile, high agitation rates narrowed the differences in culture performance between low and high working volumes. These differences were also reflected in cell metabolism, where low culture volumes enhanced oxidative metabolism (linked to a productive phenotype) and high culture volume generated a metabolic profile that was predominately glycolytic (linked to a proliferative phenotype). Our findings indicate that the culture volume influence on metabolism modulates the balance between cell growth and protein production, a key feature that may be useful to adjust CHO cells towards a more productive phenotype.

 

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red (Powder)
MBS653165-10L 10L
EUR 365

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red (Powder)
MBS653165-25L 25L
EUR 665

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red (Powder)
MBS653165-50L 50L
EUR 1285

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red (Powder)
MBS653165-5x50L 5x50L
EUR 5145

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red w/o Riboflavin (Powder)
MBS653037-10L 10L
EUR 365

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red w/o Riboflavin (Powder)
MBS653037-25L 25L
EUR 665

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red w/o Riboflavin (Powder)
MBS653037-50L 50L
EUR 1285

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red w/o Riboflavin (Powder)
MBS653037-5x50L 5x50L
EUR 5145

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red w/o Manganese (Powder)
MBS653209-10L 10L
EUR 365

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red w/o Manganese (Powder)
MBS653209-25L 25L
EUR 665

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red w/o Manganese (Powder)
MBS653209-50L 50L
EUR 1285

Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red w/o Manganese (Powder)
MBS653209-5x50L 5x50L
EUR 5145

Synthetic Sewage Medium
M5475-500G 1 unit
EUR 44.97
Description: Synthetic Sewage Medium

(±)-cistrans-Abscisic acid (synthetic)
A068-100MG 100 mg
EUR 108

(±)-cistrans-Abscisic acid (synthetic)
A068-500MG 500 mg
EUR 291.6

Polyclonal KRT5 / CK5 / Cytokeratin 5 Antibody (Synthetic peptide, corresponding to amino acids 550-600 of
APR17126G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human KRT5 / CK5 / Cytokeratin 5 (Synthetic peptide, corresponding to amino acids 550-600 of. This antibody is tested and proven to work in the following applications:

Yeast Nitrogen Base Medium without Amino Acids
HY-157347A Get quote Ask for price
Description: Yeast Nitrogen Base Medium without Amino Acids can be used in the cultivation of bacteria in genetic engineering[1].

(+/-)-Abscisic Acid Synthetic (ABA) for tissue culture, 98%
31240 25 Mg
EUR 12.6
Description: Part B

RPMI 1640 Medium w/o Amino Acids, Sodium Phosphate (Powder)
MBS653023-100L 100L
EUR 1405

RPMI 1640 Medium w/o Amino Acids, Sodium Phosphate (Powder)
MBS653023-10L 10L
EUR 310

RPMI 1640 Medium w/o Amino Acids, Sodium Phosphate (Powder)
MBS653023-25L 25L
EUR 570

RPMI 1640 Medium w/o Amino Acids, Sodium Phosphate (Powder)
MBS653023-50L 50L
EUR 930

RPMI 1640 Medium w/o Amino Acids, Sodium Phosphate (Powder)
MBS653023-5x100L 5x100L
EUR 5280

Basal Medium Eagle (BME) 100X Amino Acids, w/o Glutamine (Powder)
MBS653398-100L 100L
EUR 615

Basal Medium Eagle (BME) 100X Amino Acids, w/o Glutamine (Powder)
MBS653398-50L 50L
EUR 430

Basal Medium Eagle (BME) 100X Amino Acids, w/o Glutamine (Powder)
MBS653398-5x100L 5x100L
EUR 1710

White Modified S-3 Medium w/ Vitamins, Amino acids & Sucrose; w
PT015-10X1L 1 unit
EUR 14.48
Description: White Modified S-3 Medium w/ Vitamins, Amino acids & Sucrose; w/ Vitamins, Amino acids & Sucrose; w/ Vitamins, Amino acids & Sucrose; w/ Vitamins, Amino acids & Sucrose

White Modified S-3 Medium w/ Vitamins, Amino acids & Sucrose; w
PT015-25L 1 unit
EUR 23.69
Description: White Modified S-3 Medium w/ Vitamins, Amino acids & Sucrose; w/ Vitamins, Amino acids & Sucrose; w/ Vitamins, Amino acids & Sucrose; w/ Vitamins, Amino acids & Sucrose

White Modified S-3 Medium w/ Vitamins, Amino acids & Sucrose; w
PT015-5L 1 unit
EUR 6.3
Description: White Modified S-3 Medium w/ Vitamins, Amino acids & Sucrose; w/ Vitamins, Amino acids & Sucrose; w/ Vitamins, Amino acids & Sucrose; w/ Vitamins, Amino acids & Sucrose

PLGA poly(lactic-co-glycolic) acid); synthetic; (50% lactic and 50% glycolic acid, ~30-60 Kda)
AV-2010-1 0.1 g
EUR 226.8

PLGA poly(lactic-co-glycolic) acid); synthetic; (65% lactic and 35% glycolic acid, ~ 40-75 Kda)
AV-2020-1 1 g
EUR 226.8

PLGA poly(lactic-co-glycolic) acid); synthetic; (75% lactic and 25% glycolic acid, ~ 65-105 Kda)
AV-2030-1 1 g
EUR 270

Williams' Medium E w/o L-Glutamine, w/o Amino Acids (Powder)
MBS652999-100L 100L
EUR 810

Williams' Medium E w/o L-Glutamine, w/o Amino Acids (Powder)
MBS652999-50L 50L
EUR 625

Williams' Medium E w/o L-Glutamine, w/o Amino Acids (Powder)
MBS652999-5x100L 5x100L
EUR 2600

Mammary Epithelial Basal Medium MCDB 170 w/o Glucose, Amino Acids
PG106 4X 500ml bottle
EUR 0.1

Orcein, Synthetic
O03048 1G
EUR 141.71
Description: CAS N° 1400-62-0

Antipain (Synthetic)
MBS407511-100mg 100mg
EUR 435

Antipain (Synthetic)
MBS407511-25mg 25mg
EUR 225

Antipain (Synthetic)
MBS407511-5x100mg 5x100mg
EUR 1910

Antipain (Synthetic)
MBS407511-5x25mg 5x25mg
EUR 960

Cerulenin (Synthetic)
C278590 100mg
EUR 19000
Description: 17397-89-6

Leupeptin (synthetic)
MBS566141-100mg 100mg
EUR 650

Leupeptin (synthetic)
MBS566141-25mg 25mg
EUR 250

Leupeptin (synthetic)
MBS566141-5mg 5mg
EUR 150

Leupeptin (synthetic)
MBS566141-5x100mg 5x100mg
EUR 2885

Leupeptin (Synthetic)
MBS407318-100mg 100mg
EUR 730

Leupeptin (Synthetic)
MBS407318-25mg 25mg
EUR 285

Leupeptin (Synthetic)
MBS407318-5x100mg 5x100mg
EUR 3250

Leupeptin (Synthetic)
MBS407318-5x25mg 5x25mg
EUR 1230

Leupeptin (Synthetic)
MBS407513-100mg 100mg
EUR 280

Leupeptin (Synthetic)
MBS407513-1g 1g
EUR 1490

Leupeptin (Synthetic)
MBS407513-25mg 25mg
EUR 175

Leupeptin (Synthetic)
MBS407513-5x100mg 5x100mg
EUR 1195

Leupeptin (Synthetic)
MBS407513-5x25mg 5x25mg
EUR 745

Psychosine (Synthetic)
MBS393535-5mg 5mg
EUR 555

Psychosine (Synthetic)
MBS393535-5x5mg 5x5mg
EUR 2095

Lactacystin (Synthetic)
1709-200 each
EUR 379.2

Lactacystin (Synthetic)
A2583-.1 100 µg
EUR 106.4
Description: Ubiquitination/ Proteasome|Proteasome

Lactacystin (Synthetic)
A2583-.5 500 µg
EUR 450.4
Description: Ubiquitination/ Proteasome|Proteasome

Lactacystin (Synthetic)
A2583-1 1 mg
EUR 786.4
Description: Ubiquitination/ Proteasome|Proteasome

Resveratrol, synthetic
GP5884 100mg
EUR 115.8

Resveratrol, synthetic
GP5884-1 1
EUR 126.5

Resveratrol, synthetic
GP5884-100 100
EUR 28.4

Resveratrol, synthetic
GP5884-100MG 100 mg
EUR 70.8

Resveratrol, synthetic
GP5884-1G 1 g
EUR 189.6

Resveratrol, synthetic
GP5884-250 250
EUR 57

Resveratrol, synthetic
GP5884-250MG 250 mg
EUR 104.4

Swainsonine (Synthetic)
S885000 1mg
EUR 312
Description: 72741-87-8

Hydrotalcite, synthetic
H714835 100g
EUR 98
Description: 11097-59-9

BME 100X Amino Acids for Basal Medium Eagle (Modified). W/O L-glutamine.
BML02-500ML 500 ml
EUR 124.8
Description: 100X Amino Acids for Basal Medium Eagle (Modified). Without L-glutamine.

Williams' Medium E, w/o L-Glutamine, w/o Amino Acids, Glucose (Powder)
MBS6506786-100L 100L
EUR 810

Williams' Medium E, w/o L-Glutamine, w/o Amino Acids, Glucose (Powder)
MBS6506786-50L 50L
EUR 625

Williams' Medium E, w/o L-Glutamine, w/o Amino Acids, Glucose (Powder)
MBS6506786-5x100L 5x100L
EUR 2600

Synthetic Sea Salt
M1344-500G 1 unit
EUR 45.2
Description: Synthetic Sea Salt

Geranylgeraniol (Synthetic)
G367501 500mg
EUR 207
Description: 24034-73-9

Atpenin A5 (Synthetic)
2210-1000 each
EUR 548.4

Atpenin A5 (Synthetic)
2210-250 each
EUR 229.2

Synthetic Gastrin (GT)
RPU56912-100ug 100ug
EUR 470.4

Synthetic Gastrin (GT)
RPU56912-1mg 1mg
EUR 2184

Synthetic Gastrin (GT)
RPU56912-50ug 50ug
EUR 385

RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose (Powder)
MBS652918-100L 100L
EUR 1405

RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose (Powder)
MBS652918-10L 10L
EUR 310

RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose (Powder)
MBS652918-25L 25L
EUR 570

RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose (Powder)
MBS652918-50L 50L
EUR 930

RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose (Powder)
MBS652918-5x100L 5x100L
EUR 5280

Synthetic BNP protein
MBS5308540-1mg 1mg
EUR 690

Synthetic BNP protein
MBS5308540-5x1mg 5x1mg
EUR 3070

Synthetic Apelin (APLN)
RPU57189-100ug 100ug
EUR 470.4

Synthetic Apelin (APLN)
RPU57189-1mg 1mg
EUR 2184

Synthetic Apelin (APLN)
RPU57189-50ug 50ug
EUR 385

Menthol Synthetic pure
64370 100 Gms
EUR 6.5
Description: Part A

Richard's Synthetic Agar
M693-500G 1 unit
EUR 44.15
Description: Richard's Synthetic Agar

Pepstatin A (synthetic)
MBS566175-100mg 100mg
EUR 530

Pepstatin A (synthetic)
MBS566175-25mg 25mg
EUR 235

Pepstatin A (synthetic)
MBS566175-5mg 5mg
EUR 155

Pepstatin A (synthetic)
MBS566175-5x100mg 5x100mg
EUR 2315

Pepstatin A (Synthetic)
MBS407516-100mg 100mg
EUR 350

Pepstatin A (Synthetic)
MBS407516-1g 1g
EUR 2135

Pepstatin A (Synthetic)
MBS407516-25mg 25mg
EUR 200

Pepstatin A (Synthetic)
MBS407516-5x100mg 5x100mg
EUR 1525

Pepstatin A (Synthetic)
MBS407516-5x25mg 5x25mg
EUR 845

Epothilone B (synthetic)
E588990 10mg
EUR 117
Description: 152044-54-7

Epothilone D (synthetic)
E589000 10mg
EUR 552
Description: 189453-10-9

Balsam canada, Synthetic
GRM322-500G 1 unit
EUR 36.15
Description: Balsam canada, Synthetic

Synthetic NEDD8 protein
MBS5308839-0025mg 0.025mg
EUR 460

Synthetic NEDD8 protein
MBS5308839-5x0025mg 5x0.025mg
EUR 2035

Synthetic Calcitonin (CT)
MBS2123983-001mg 0.01mg
EUR 155

Synthetic Calcitonin (CT)
MBS2123983-005mg 0.05mg
EUR 260

Synthetic Calcitonin (CT)
MBS2123983-01mg 0.1mg
EUR 375

Synthetic Calcitonin (CT)
MBS2123983-02mg 0.2mg
EUR 460

Synthetic Calcitonin (CT)
MBS2123983-05mg 0.5mg
EUR 860

Synthetic Calcitonin (CT)
RPU56173-100ug 100ug
EUR 554.4

Synthetic Calcitonin (CT)
RPU56173-1mg 1mg
EUR 2184

Synthetic Calcitonin (CT)
RPU56173-50ug 50ug
EUR 336

Synthetic Antide protein
MBS5308514-1mg 1mg
EUR 480

Synthetic Antide protein
MBS5308514-5x1mg 5x1mg
EUR 2115

Synthetic Neurotensin (NT)
MBS2097730-001mg 0.01mg
EUR 140

Synthetic Neurotensin (NT)
MBS2097730-005mg 0.05mg
EUR 235

Synthetic Neurotensin (NT)
MBS2097730-01mg 0.1mg
EUR 325

Synthetic Neurotensin (NT)
MBS2097730-02mg 0.2mg
EUR 400

Synthetic Neurotensin (NT)
MBS2097730-05mg 0.5mg
EUR 745

Synthetic Neurotensin (NT)
RPU55424-100ug 100ug
EUR 369.6

Synthetic Neurotensin (NT)
RPU55424-1mg 1mg
EUR 1638

Synthetic Neurotensin (NT)
RPU55424-50ug 50ug
EUR 297

b-Sitosterol (Synthetic)
S497050 10mg
EUR 236
Description: 83-46-5

Synthetic Neurotensin (NT)
SPB203Hu01 10ug
EUR 120

Synthetic Neurotensin (NT)
4-SPB203Hu01
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  • 5 mg
  • 10ug
  • 50ug
  • 100 ug
  • 200 ug
  • 500 ug
  • 1 mg
Description: Recombinant Human Neurotensin expressed in: E.coli

SYNTHETIC HUMAN CEREBELLIN
GWB-7C45AE 0.1 mg Ask for price

Sphistin Synthetic Peptide
MBS5763362-1mg 1mg
EUR 235

Sphistin Synthetic Peptide
MBS5763362-5mg 5mg
EUR 520

Sphistin Synthetic Peptide
MBS5763362-5x5mg 5x5mg
EUR 2195

Synthetic Secretin protein
MBS5308933-1mg 1mg
EUR 455

Synthetic Secretin protein
MBS5308933-5x1mg 5x1mg
EUR 2005

Sphistin Synthetic Peptide
TP1036-10mg 10mg Ask for price
Description: Sphistin Synthetic Peptide

Sphistin Synthetic Peptide
TP1036-1g 1g Ask for price
Description: Sphistin Synthetic Peptide

Sphistin Synthetic Peptide
TP1036-1mg 1mg Ask for price
Description: Sphistin Synthetic Peptide

Sphistin Synthetic Peptide
TP1036-50mg 50mg Ask for price
Description: Sphistin Synthetic Peptide

Sphistin Synthetic Peptide
TP1036-5mg 5mg Ask for price
Description: Sphistin Synthetic Peptide

SYNTHETIC HUMAN OSTEOPONTIN
GWB-9D4DD0 0.05 mg Ask for price

Synthetic Ubiquitin protein
MBS5309003-0025mg 0.025mg
EUR 460

Synthetic Ubiquitin protein
MBS5309003-5x0025mg 5x0.025mg
EUR 2035

Synthetic Elcatonin protein
MBS5308624-02mg 0.2mg
EUR 455

Synthetic Elcatonin protein
MBS5308624-5x02mg 5x0.2mg
EUR 2005

Synthetic HIV-2 gp36
MBS142593-01mg 0.1mg
EUR 250

Synthetic HIV-2 gp36
MBS142593-05mg 0.5mg
EUR 455

Synthetic HIV-2 gp36
MBS142593-1mg 1mg
EUR 660

Synthetic HIV-2 gp36
MBS142593-5x1mg 5x1mg
EUR 2640

Synthetic HIV-2 gp36
rAP-5340 Inquiry Ask for price

Synthetic Leuprolide protein
MBS5308799-5mg 5mg
EUR 510

Synthetic Leuprolide protein
MBS5308799-5x5mg 5x5mg
EUR 2245

Synthetic Mucin 1 (MUC1)
MBS2086946-001mg 0.01mg
EUR 140

Synthetic Mucin 1 (MUC1)
MBS2086946-005mg 0.05mg
EUR 235

Synthetic Mucin 1 (MUC1)
MBS2086946-01mg 0.1mg
EUR 325

Synthetic Mucin 1 (MUC1)
MBS2086946-02mg 0.2mg
EUR 400

Synthetic Mucin 1 (MUC1)
MBS2086946-05mg 0.5mg
EUR 745

Synthetic Mucin 1 (MUC1)
RPU50301-100ug 100ug
EUR 369.6

Synthetic Mucin 1 (MUC1)
RPU50301-1mg 1mg
EUR 1638

Synthetic Mucin 1 (MUC1)
RPU50301-50ug 50ug
EUR 297

Synthetic Mucin 1 (MUC1)
SPA413Hu02 10ug
EUR 120

Synthetic Mucin 1 (MUC1)
4-SPA413Hu02
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  • 10ug
  • 50ug
  • 100 ug
  • 200 ug
  • 500 ug
  • 1 mg
  • 5 mg
Description: Recombinant Human Mucin 1 expressed in: E.coli

PLGA poly(lactic-co-glycolic) acid); synthetic (75% lactic and 25% glycolic acid; Ester capped, , MW 75-115 Kda)
AV-2040-1 1 g
EUR 196.8
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