Optimization of Viable Glioblastoma Cryopreservation for Establishment of Primary Tumor Cell Cultures

eaaci2015

Development of new media formulations for cell culture operations based on regression models

The paper discusses modelling and optimization of multi-component cell culture medium. The specific productivity (Qp) was considered a function of the medium components and possible interactions described by linear factors, two-way interactions and squared terms that results in a high dimensional problem where the number of variables p (represented by the medium components and their interactions) is much larger than the number of observations n.
Principal Components Regression (PCR), Partial Least Squares (PLS), Lasso and Elastic Net regressions were compared as modelling tools to deal with a high dimensional [Formula: see text] problem. PCR and PLS regression models resulted in better prediction results and were used for robust optimization of the medium composition by a nonlinear optimization. The case studies show that it is possible to formulate new media that result in higher Qp than the ones provided by the initial media experiments available. Also, the multivariate statistical approach permitted us to select media that is most informative about the optimum thus permitting modelling and optimization with a reduced set of initial experiments.

Heat Stress Triggers Differential Protein Accumulation in the Extracellular Matrix of Sorghum Cell Suspension Cultures

Plants reprogram gene expression as an adaptive response to survive high temperatures. While the identity and functions of intracellular heat stress-responsive proteins have been extensively studied, the heat response of proteins secreted to the extracellular matrix is unknown. Here, we used Sorghum bicolor, a species adapted for growth in hot climates, to investigate the extracellular heat-induced responses. When exposed to 40 C for 72 h, heat-sensitive Arabidopsis cell suspension cultures died, while ICSB338 sorghum cell cultures survived by activation of a transcriptional response characterized by the induction of HSP70 and HSP90 genes.
Quantitative proteomic analysis of proteins recovered from cell culture medium revealed specific heat stress-induced protein accumulation within the sorghum secretome. Of the 265 secreted proteins identified, 31 responded to heat (2-fold change), with 84% possessing a predicted signal peptide for targeting to the classical secretory pathway. The differentially accumulated proteins have putative functions in metabolism, detoxification, and protein modifications. A germin (SORBI_3003G427700) was highly heat-inducible at both protein and gene level. Overall, our study reveals new insights into sorghum responses to heat and provides a useful resource of extracellular proteins that could serve as targets for developing thermotolerant crops. Data are available via ProteomeXchange with identifier PXD021536.

Xenograft and cell culture models of Sézary syndrome reveal cell of origin diversity and subclonal heterogeneity

Sézary Syndrome (SS) is a rare aggressive epidermotropic cutaneous T-cell lymphoma (CTCL) defined by erythroderma, pruritis, and a circulating atypical CD4 + T-cell clonal population. The diversity of Sézary cell (SC) phenotype and genotype may reflect either plasticity or heterogeneity, which was difficult to evaluate dynamically until the achievement of long-term SC expansion. Therefore, we developed six defined culture conditions allowing for the expansion of SC defined by their phenotype and monoclonality in four of seven SS cases. Engraftment of SC through the intrafemoral route into immunodeficient NOD.Cg-Prkdc(scid)Il2rg(tm1Wjll)/SzJ (NSG) mice was achieved in 2 of 14 SS cases. Secondary xenograft by percutaneous injection mimicked most of the features of SS with dermal infiltration, epidermotropism, and blood spreading.
These models also allowed assessing the intra-individual heterogeneity of patient SC. Subclones sharing the same TCR gene rearrangement evolved independently according to culture conditions and/or after xenografting. This clonal selection was associated with some immunophenotypic plasticity and limited genomic evolution both in vitro and in vivo. The long-term amplification of SC allowed us to develop eight new SC lines derived from four different patients. These lines represent the cell of origin diversity of SC and provide new tools to evaluate their functional hallmarks and response to therapy.
eaaci2015

eaaci2015

Hamster Red Blood Cells

88R-H003 10 ml
EUR 489
Description: Washed and freshly prepared 10% suspension of Hamster Red Blood Cells

Mouse Red Blood Cells

88R-M001 10 ml
EUR 295
Description: Washed and freshly prepared 10% suspension of Mouse Red Blood Cells

Monkey Red Blood Cells

88R-M003 5 x 2 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Monkey Red Blood Cells

Mouse Red Blood Cells

88R-M004 5 x 2 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Mouse Red Blood Cells

Pig Red Blood Cells

88R-P002 20 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Pig Red Blood Cells

Rabbit Red Blood Cells

88R-R001 20 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Rabbit Red Blood Cells

Rat Red Blood Cells

88R-R002 5 x 2.0ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Rat Red Blood Cells

Rat Red Blood Cells

88R-R005 25 ml
EUR 327
Description: Washed and freshly prepared 10% suspension of Rat Red Blood Cells

Sheep Red Blood Cells

88R-S001 100 ml
EUR 512
Description: Washed and freshly prepared 10% suspension of Sheep Red Blood Cells

Sheep Red Blood Cells

88R-S003 20 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Sheep Red Blood Cells

Sheep Red Blood Cells

88R-S004 10 ml
EUR 521
Description: Glutaraldehyde-stabilized freshly prepared albumin sensitised Sheep Red Blood Cells

Sheep Red Blood Cells

88R-S005 10 ml
EUR 446
Description: Glutaraldehyde-stabilized freshly prepared tanned Sheep Red Blood Cells

Turkey Red Blood Cells

88R-T001 20 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Turkey Red Blood Cells

Sheep Red Blood Cells

20R-RS001 10 ml
EUR 521
Description: Human IgG Senstitized Gluteraldehyde Stabilized Sheep Red Bllood Cells

Rabbit Red Blood Cells (100%)

20-abx800015
  • EUR 648.00
  • EUR 509.00
  • 100 ml
  • 50 ml

Sheep Red Blood Cells (100%)

20-abx800032
  • EUR 356.00
  • EUR 314.00
  • 100 ml
  • 50 ml

Goat Red Blood Cells (100%)

20-abx800047
  • EUR 384.00
  • EUR 328.00
  • 100 ml
  • 50 ml

Horse Red Blood Cells (100%)

20-abx800062
  • EUR 453.00
  • EUR 551.00
  • EUR 384.00
  • 100 ml
  • 500 ml
  • 50 ml

Horse Red Blood Cells (100%)

abx800062-1L 1 L
EUR 746

Donkey Red Blood Cells (100%)

20-abx800077
  • EUR 676.00
  • EUR 843.00
  • EUR 481.00
  • 100 ml
  • 500 ml
  • 50 ml

Donkey Red Blood Cells (100%)

abx800077-1L 1 L
EUR 1191

Camel Red Blood Cells (100%)

20-abx800092
  • EUR 746.00
  • EUR 1636.00
  • EUR 578.00
  • 100 ml
  • 500 ml
  • 50 ml

Camel Red Blood Cells (100%)

abx800092-1L 1 L
EUR 3042

Dog Red Blood Cells (100%)

20-abx800122
  • EUR 592.00
  • EUR 481.00
  • 100 ml
  • 50 ml

Chicken Red Blood Cells (100%)

20-abx800137
  • EUR 384.00
  • EUR 328.00
  • 100 ml
  • 50 ml

Guinea Pig Red Blood Cells

88R-1047 15 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Guinea Pig Red Blood Cells

Guinea Pig Red Blood Cells

88R-G002 25 ml
EUR 365
Description: Washed and freshly prepared 10% suspension of Guinea Pig Red Blood Cells

Guinea Pig Red Blood Cells

88R-P001 15 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Guinea Pig Red Blood Cells

Human Cord Blood CD34+ Cells Derived Endothelial Cells

HEC23 500,000+ Cells
EUR 1080

Neural Stem Cells (iPSC from Blood Cells; Male)

ASE-9234 1 vial (1 x 10^6)
EUR 414.5
Description: 12 month

Neural Stem Cells (iPSC from Blood Cells; Female)

ASE-9234F 1 vial (1 x 10^6)
EUR 414.5
Description: 12 month

Rabbit RED BLOOD CELLS HEPARIN 500mL*

31159-5 500mL
EUR 821.9

Beagle dog Red Blood Cells (100%)

20-abx800107
  • EUR 829.00
  • EUR 662.00
  • 100 ml
  • 50 ml

Neurons (iPSC from Blood Cells; Male)

ASE-9321 1 vial (1 x 10^6)
EUR 313.25
Description: 12 month

Neurons (iPSC from Blood Cells; Female)

ASE-9321F 1 vial (1 x 10^6)
EUR 313.25
Description: 12 month

Astrocytes Mature (iPSC from Blood Cells; Male)

ASE-9322M 1 vial (1 x 10^6)
EUR 455
Description: 12 month

Astrocytes Mature (iPSC from Blood Cells; Female)

ASE-9322MF 1 vial (1 x 10^6)
EUR 455
Description: 12 month

Astrocytes Precursors (iPSC from Blood Cells; Male)

ASE-9322P 1 vial (1 x 10^6)
EUR 374
Description: 12 month

Astrocytes Precursors (iPSC from Blood Cells; Female)

ASE-9322PF 1 vial (1 x 10^6)
EUR 374
Description: 12 month

Dopaminergic Neurons (iPSC from Blood Cells; Male)

ASE-9323 1 vial
EUR 374
Description: 12 month

Dopaminergic Neurons (iPSC from Blood Cells; Female)

ASE-9323F 1 vial
EUR 374
Description: 12 month

Lysis-EZ L, White Blood Cell Lysis Buffer, 1X Strength Solution

PL015 10mL
EUR 58.7

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh

BMC-F500M - Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, single donor

CBCD34-C0.5M - Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, single donor 

CBCD34-C1M - Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor 

CBCD34mix-C0.5M - Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor 

CBCD34mix-C1M - Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor 

CBCD34mix-C5M - Ask for price

Human Peripheral Blood CD3+ T Cells, Cryopreserved

PBCD3-C10M - Ask for price

Human Peripheral Blood CD3+ T Cells, Cryopreserved

PBCD3-C20M - Ask for price

Human Peripheral Blood CD3+ T Cells, Fresh

PBCD3-F20M - Ask for price

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved

PBMC-C100M - Ask for price

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved

PBMC-C10M - Ask for price

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved

PBMC-C20M - Ask for price

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved

PBMC-C50M - Ask for price

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh

PBMC-F100M - Ask for price

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh

PBMC-F1B - Ask for price

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh

PBMC-F2B - Ask for price

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh

PBMC-F300M - Ask for price

Neurons Starter Kit (iPSC from Blood Cells; Male)

ASE-9321K 1 Kit
EUR 394.25
Description: 12 month

Neurons Starter Kit (iPSC from Blood Cells; Female)

ASE-9321KF 1 Kit
EUR 394.25
Description: 12 month

white plates

1270-20
EUR 98

Mouse Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells

2-82072 1 Kit Ask for price

Rat Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells

2-82571 1 Kit Ask for price

Human Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells

2-96088 1 Kit Ask for price

Positively Selected Normal Peripheral Blood CD19+ B Cells, Cryopreserved

PBCD19P-C10M - Ask for price

Astrocytes Precursors Starter Kit (iPSC from Blood Cells; Male)

ASE-9322PK 1 Kit
EUR 414.5
Description: 12 month

Astrocytes Precursors Starter Kit (iPSC from Blood Cells; Female)

ASE-9322PKF 1 Kit
EUR 414.5
Description: 12 month

Dopaminergic Neurons Starter Kit (iPSC from Blood Cells; Male)

ASE-9323K 1 vial
EUR 455
Description: 12 month

Dopaminergic Neurons Starter Kit (iPSC from Blood Cells; Female)

ASE-9323KF 1 vial
EUR 455
Description: 12 month

Positively Selected Normal Peripheral Blood CD19+ B Cells, Fresh

PBCD19P-F10M - Ask for price

Lysozyme (Egg White)

abx082183-5g 5 g
EUR 189

Lysozyme (Egg White)

abx082564-5g 5 g
EUR 203

Labeling Tape, White

TP003W 1ROLLS, 1 UNIT
EUR 53.68

Lysozyme, Egg White

L2100-005 5g
EUR 174

Lysozyme, Egg White

L2100-010 10g
EUR 219

Lysozyme, Egg White

L2100-100 100g
EUR 583

Safe-White™

G108-W 1.0 ml
EUR 71

Rat Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells Growth Medium

9-25071 5 x 100 ml Ask for price

Mouse Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells Growth Medium

9-32072 5 x 100 ml Ask for price

Optimization of Viable Glioblastoma Cryopreservation for Establishment of Primary Tumor Cell Cultures

 

Background: Technologies related to the establishment of primary tumor cell cultures from solid tumors, including glioblastoma, are increasingly important to oncology research and practice. However, processing of fresh tumor specimens for establishment of primary cultures on the day of surgical collection is logistically difficult. The feasibility of viable cryopreservation of glioblastoma specimens, allowing for primary culture establishment weeks to months after surgical tumor collection and freezing, was demonstrated by Mullins et al. in 2013, with a success rate of 59% that was not significantly lower than that achieved with fresh tumor tissue. However, research targeting optimization of viable glioblastoma cryopreservation protocols for establishment of primary tumor cultures has been limited.
Objectives: The objective of this study was to optimize glioblastoma cryopreservation methods for viable cryobanking and to determine if two-dimensional (2D) or three-dimensional (3D) culture conditions were more supportive of glioblastoma growth after thawing of frozen tumor specimens.
Methods: Portions of eight human glioblastoma specimens were cryopreserved by four different protocols differing in the time of enzymatic digestion (before or after cryopreservation), and in the type of cryopreservation media (CryoStor CS10 or 10% dimethyl sulfoxide and 90% fetal calf serum). After 1 month, frozen tissues were thawed, enzymatically digested, if not digested before, and used for initiation of 2D or 3D primary tumor cultures to determine viability.
Results: Among the tested cryopreservation and culturing protocols, the most efficient combinations of cryopreservation and culture were those associated with the use of CryoStor CS10 cryopreservation medium, enzymatic digestion before freezing, and 2D culturing after thawing with a successful culture rate of 8 out of 8 cases (100%). Two-dimensional cultures were in general more efficient for the support of tumor cell growth after thawing than 3D cultures.

Metformin partially reverses the inhibitory effect of co-culture with ER-/PR-/HER2+ breast cancer cells on biomarkers of monocyte antitumor activity

Background: Immune activities of monocytes (MOs) can be altered within the microenvironment of solid malignancies, including breast cancer. Metformin (1,1-dimethylbiguanide hydrochloride, MET), has been shown to decrease tumor cell proliferation, but its effects have yet to be explored with respect to MOs (monocytes) activity during their crosstalk with breast cancer cells. Here, we investigated the effects of MET on overall phenotypic functional activities, including cellular immunometabolism and protective redox signaling based-biomarkers, intracellular free calcium ions (ifCa2+), phagocytosis and co-operative cytokines (IFN-γ and IL-10) of autologous MOs before and during their interplay with primary ER-/PR-/HER2+ breast cancer cells.
Methods: Human primary breast cancer cells were either cultured alone or co-cultured with autologous MOs before treatment with MET.
Results: MET downregulated breast cancer cell proliferation and phagocytosis, while having no significant effect on the ratio of phosphorylated Akt (p-Akt) to total Akt. Additionally, we observed that, in the absence of MET treatment, the levels of lactate dehydrogenase (LDH)-based cytotoxicity, catalase, ifCa2+, IL-10 and arginase activity were significantly reduced in co-cultures compared to levels in MOs cultured alone whereas levels of inducible nitric oxide synthase (iNOS) activity were significantly increased. In contrast, MET treatment reduced the effects measured in co-culture on the levels of LDH-based cytotoxicity, arginase activity, catalase, ifCa2+, and IFN-γ. MET also induced upregulation of both iNOS and arginase in MO cells, although the increase did not reach significant difference for iNOS activity.
Moreover, MET induced a robust increase of superoxide dismutase (SOD) activity in MOs, but not in MOs co-cultured with breast cancer cells. Furthermore, MET markedly upregulated the levels of IFN-γ production and downregulated those of IL-10 in isolated MOs, while inducing a slight opposing up-regulation of IL-10 production in co-cultures.
MagIso CD8 Memory T Cell Isolation Kit, Human
WHK-B026 20 tests, 200 tests Ask for price
Human Normal Peripheral Blood CD4+/CD25+ Regulatory T Cells (T reg), Cryopreserved
PBCD4-25-C2M - Ask for price
Human Normal Peripheral Blood CD4+/CD25+ Regulatory T Cells (T reg), Fresh
PBCD4-25-F2M - Ask for price
Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-10 Ask for price
Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-20 Ask for price
Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-30 Ask for price
Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-40 Ask for price
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx125313
  • EUR 495.00
  • EUR 704.00
  • EUR 356.00
  • 100 ul
  • 200 ul
  • 50 ul
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx111482
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx134635
  • EUR 356.00
  • EUR 565.00
  • EUR 300.00
  • 100 ul
  • 200 ul
  • 50 ul
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx134645
  • EUR 356.00
  • EUR 565.00
  • EUR 300.00
  • 100 ul
  • 200 ul
  • 50 ul
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx134646
  • EUR 356.00
  • EUR 565.00
  • EUR 300.00
  • 100 ul
  • 200 ul
  • 50 ul
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx137099
  • EUR 356.00
  • EUR 523.00
  • 0.5 mg
  • 1 mg
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx012383-100ul 100 ul
EUR 411
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx009476
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx000694
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx001304
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx015800-100ul 100 ul
EUR 411
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx019049-100ug 100 ug
EUR 342
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx021410-02mg 0.2 mg
EUR 739
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx028455-400ul 400 ul
EUR 523
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx028455-80l 80 µl
EUR 286
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx028456-400ul 400 ul
EUR 551
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx028456-80l 80 µl
EUR 321
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx412648-025mg 0.25 mg
EUR 565
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx412680-01mg 0.1 mg
EUR 509
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx432473-200ul 200 ul
EUR 384
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx412727-01mg 0.1 mg
EUR 453
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx412755-05mg 0.5 mg
EUR 829
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx412884-02mg 0.2 mg
EUR 565
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx412885-05mg 0.5 mg
EUR 829
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx412888-01mg 0.1 mg
EUR 439
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx412891-25ug 25 ug
EUR 272
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413138-01mg 0.1 mg
EUR 439
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413139-025mg 0.25 mg
EUR 565
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413254-01mg 0.1 mg
EUR 453
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413340-025mg 0.25 mg
EUR 565
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413341-05mg 0.5 mg
EUR 829
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413342-01mg 0.1 mg
EUR 439
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413343-25ug 25 ug
EUR 272
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413612-2ml 2 ml
EUR 606
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413766-01mg 0.1 mg
EUR 509
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413842-01mg 0.1 mg
EUR 453
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414255-025mg 0.25 mg
EUR 592
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414488-025mg 0.25 mg
EUR 565
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414492-25ug 25 ug
EUR 272
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414713-025mg 0.25 mg
EUR 565
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414714-1mg 1 mg
EUR 829
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414765-025mg 0.25 mg
EUR 565
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414766-05mg 0.5 mg
EUR 829
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414768-01mg 0.1 mg
EUR 439
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414771-25ug 25 ug
EUR 272
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414950-02mg 0.2 mg
EUR 565
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414951-25ug 25 ug
EUR 272
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx415067-1mg 1 mg
EUR 829
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx415068-01mg 0.1 mg
EUR 439
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx415070-025mg 0.25 mg
EUR 565
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx415071-1mg 1 mg
EUR 829
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx415352-01mg 0.1 mg
EUR 453
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx415359-01mg 0.1 mg
EUR 453
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx415473-025ml 0.25 ml
EUR 606
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx415540-01mg 0.1 mg
EUR 453
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx415565-01mg 0.1 mg
EUR 453
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx320336
  • EUR 300.00
  • EUR 244.00
  • 100 ul
  • 50 ul
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx231475-100ug 100 ug
EUR 509
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx231476-100ug 100 ug
EUR 481
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx159398
  • EUR 272.00
  • EUR 230.00
  • 100 ug
  • 50 ug
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159399-100ul 100 ul
EUR 356
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159400-100ul 100 ul
EUR 356
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159401-100ul 100 ul
EUR 356
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159402-100ul 100 ul
EUR 356
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159403-100ul 100 ul
EUR 439
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159404-100ul 100 ul
EUR 356
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159405-100ul 100 ul
EUR 356
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159406-100ul 100 ul
EUR 356
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx146722-100ug 100 ug
EUR 439
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx146728-100ug 100 ug
EUR 439
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx200030-100ug 100 ug
EUR 328
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx200854
  • EUR 272.00
  • EUR 411.00
  • 100 ug
  • 500 ug
T-Cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx242941
  • EUR 467.00
  • EUR 328.00
  • 100 ul
  • 50 ul
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx139125-01mg 0.1 mg
EUR 356
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx139126-01mg 0.1 mg
EUR 356
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx139127-01mg 0.1 mg
EUR 356
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx140007-01mg 0.1 mg
EUR 286
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx140008-01mg 0.1 mg
EUR 286
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx140009-01mg 0.1 mg
EUR 286
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx140102-01mg 0.1 mg
EUR 314
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx140341-01mg 0.1 mg
EUR 356
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx140432-01mg 0.1 mg
EUR 356
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx139124-01mg 0.1 mg
EUR 356