Optimization of Viable Glioblastoma Cryopreservation for Establishment of Primary Tumor Cell Cultures

November 12, 2020
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eaaci2015

Development of new media formulations for cell culture operations based on regression models

The paper discusses modelling and optimization of multi-component cell culture medium. The specific productivity (Qp) was considered a function of the medium components and possible interactions described by linear factors, two-way interactions and squared terms that results in a high dimensional problem where the number of variables p (represented by the medium components and their interactions) is much larger than the number of observations n.
Principal Components Regression (PCR), Partial Least Squares (PLS), Lasso and Elastic Net regressions were compared as modelling tools to deal with a high dimensional [Formula: see text] problem. PCR and PLS regression models resulted in better prediction results and were used for robust optimization of the medium composition by a nonlinear optimization. The case studies show that it is possible to formulate new media that result in higher Qp than the ones provided by the initial media experiments available. Also, the multivariate statistical approach permitted us to select media that is most informative about the optimum thus permitting modelling and optimization with a reduced set of initial experiments.

Heat Stress Triggers Differential Protein Accumulation in the Extracellular Matrix of Sorghum Cell Suspension Cultures

Plants reprogram gene expression as an adaptive response to survive high temperatures. While the identity and functions of intracellular heat stress-responsive proteins have been extensively studied, the heat response of proteins secreted to the extracellular matrix is unknown. Here, we used Sorghum bicolor, a species adapted for growth in hot climates, to investigate the extracellular heat-induced responses. When exposed to 40 C for 72 h, heat-sensitive Arabidopsis cell suspension cultures died, while ICSB338 sorghum cell cultures survived by activation of a transcriptional response characterized by the induction of Gentaur.
Quantitative proteomic analysis of proteins recovered from cell culture medium revealed specific heat stress-induced protein accumulation within the sorghum secretome. Of the 265 secreted proteins identified, 31 responded to heat (2-fold change), with 84% possessing a predicted signal peptide for targeting to the classical secretory pathway. The differentially accumulated proteins have putative functions in metabolism, detoxification, and protein modifications. A germin (SORBI_3003G427700) was highly heat-inducible at both protein and gene level. Overall, our study reveals new insights into sorghum responses to heat and provides a useful resource of extracellular proteins that could serve as targets for developing thermotolerant crops. Data are available via ProteomeXchange with identifier PXD021536.

Xenograft and cell culture models of Sézary syndrome reveal cell of origin diversity and subclonal heterogeneity

Sézary Syndrome (SS) is a rare aggressive epidermotropic cutaneous T-cell lymphoma (CTCL) defined by erythroderma, pruritis, and a circulating atypical CD4 + T-cell clonal population. The diversity of Sézary cell (SC) phenotype and genotype may reflect either plasticity or heterogeneity, which was difficult to evaluate dynamically until the achievement of long-term SC expansion. Therefore, we developed six defined culture conditions allowing for the expansion of SC defined by their phenotype and monoclonality in four of seven SS cases. Engraftment of SC through the intrafemoral route into immunodeficient NOD.Cg-Prkdc(scid)Il2rg(tm1Wjll)/SzJ (NSG) mice was achieved in 2 of 14 SS cases. Secondary xenograft by percutaneous injection mimicked most of the features of SS with dermal infiltration, epidermotropism, and blood spreading.
These models also allowed assessing the intra-individual heterogeneity of patient SC. Subclones sharing the same TCR gene rearrangement evolved independently according to culture conditions and/or after xenografting. This clonal selection was associated with some immunophenotypic plasticity and limited genomic evolution both in vitro and in vivo. The long-term amplification of SC allowed us to develop eight new SC lines derived from four different patients. These lines represent the cell of origin diversity of SC and provide new tools to evaluate their functional hallmarks and response to therapy.
eaaci2015

eaaci2015

Horse Red Blood Cells
88R-H002 20 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Horse Red Blood Cells

Hamster Red Blood Cells
88R-H003 10 ml
EUR 586.8
Description: Washed and freshly prepared 10% suspension of Hamster Red Blood Cells

Mouse Red Blood Cells
88R-M001 10 ml
EUR 354
Description: Washed and freshly prepared 10% suspension of Mouse Red Blood Cells

Monkey Red Blood Cells
88R-M003 5 x 2 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Monkey Red Blood Cells

Mouse Red Blood Cells
88R-M004 5 x 2 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Mouse Red Blood Cells

Pig Red Blood Cells
88R-P002 20 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Pig Red Blood Cells

Rabbit Red Blood Cells
88R-R001 20 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Rabbit Red Blood Cells

Rat Red Blood Cells
88R-R002 5 x 2.0ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Rat Red Blood Cells

Rat Red Blood Cells
88R-R005 25 ml
EUR 392.4
Description: Washed and freshly prepared 10% suspension of Rat Red Blood Cells

Sheep Red Blood Cells
88R-S001 100 ml
EUR 614.4
Description: Washed and freshly prepared 10% suspension of Sheep Red Blood Cells

Sheep Red Blood Cells
88R-S003 20 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Sheep Red Blood Cells

Sheep Red Blood Cells
88R-S004 10 ml
EUR 625.2
Description: Glutaraldehyde-stabilized freshly prepared albumin sensitised Sheep Red Blood Cells

Sheep Red Blood Cells
88R-S005 10 ml
EUR 535.2
Description: Glutaraldehyde-stabilized freshly prepared tanned Sheep Red Blood Cells

Turkey Red Blood Cells
88R-T001 20 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Turkey Red Blood Cells

Rabbit Red Blood Cells (100%)
20-abx800015
  • EUR 777.60
  • EUR 610.80
  • 100 ml
  • 50 ml

Sheep Red Blood Cells (100%)
20-abx800032
  • EUR 427.20
  • EUR 376.80
  • 100 ml
  • 50 ml

Goat Red Blood Cells (100%)
20-abx800047
  • EUR 460.80
  • EUR 393.60
  • 100 ml
  • 50 ml

Horse Red Blood Cells (100%)
20-abx800062
  • EUR 543.60
  • EUR 661.20
  • EUR 460.80
  • 100 ml
  • 500 ml
  • 50 ml

Horse Red Blood Cells (100%)
abx800062-1L 1 L
EUR 895.2

Donkey Red Blood Cells (100%)
20-abx800077
  • EUR 811.20
  • EUR 1011.60
  • EUR 577.20
  • 100 ml
  • 500 ml
  • 50 ml

Donkey Red Blood Cells (100%)
abx800077-1L 1 L
EUR 1429.2

Camel Red Blood Cells (100%)
20-abx800092
  • EUR 895.20
  • EUR 1963.20
  • EUR 693.60
  • 100 ml
  • 500 ml
  • 50 ml

Camel Red Blood Cells (100%)
abx800092-1L 1 L
EUR 3650.4

Dog Red Blood Cells (100%)
20-abx800122
  • EUR 710.40
  • EUR 577.20
  • 100 ml
  • 50 ml

Chicken Red Blood Cells (100%)
20-abx800137
  • EUR 460.80
  • EUR 393.60
  • 100 ml
  • 50 ml

Guinea Pig Red Blood Cells
88R-1047 15 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Guinea Pig Red Blood Cells

Guinea Pig Red Blood Cells
88R-G002 25 ml
EUR 438
Description: Washed and freshly prepared 10% suspension of Guinea Pig Red Blood Cells

Guinea Pig Red Blood Cells
88R-P001 15 ml
EUR 327.6
Description: Glutaraldehyde-stabilized freshly prepared Guinea Pig Red Blood Cells

Neural Stem Cells (iPSC from Blood Cells; Male)
ASE-9234 1 vial (1 x 10^6)
EUR 497.4
Description: 12 month

Neural Stem Cells (iPSC from Blood Cells; Female)
ASE-9234F 1 vial (1 x 10^6)
EUR 497.4
Description: 12 month

Human Cord Blood CD34+ Cells Derived Endothelial Cells
HEC23 500,000+ Cells
EUR 1296

Rabbit RED BLOOD CELLS HEPARIN 500mL*
31159-5 500mL
EUR 986.28

Beagle dog Red Blood Cells (100%)
20-abx800107
  • EUR 994.80
  • EUR 794.40
  • 100 ml
  • 50 ml

Neurons (iPSC from Blood Cells; Male)
ASE-9321 1 vial (1 x 10^6)
EUR 375.9
Description: 12 month

Neurons (iPSC from Blood Cells; Female)
ASE-9321F 1 vial (1 x 10^6)
EUR 375.9
Description: 12 month

Normal Human Peripheral Blood Mononuclear Cells, Frozen -100 million cells
79059-2 100 million cells
EUR 580
Description: Cryopreserved vial (30 x 10^6 cells) of freshly isolated primary human peripheral blood mononuclear cells (PBMCs) from a healthy donor, isolated from leukapheresis / apheresis samples using Ficoll gradient. After isolation, the PBMCs were stained to identify sub populations and evaluated for viability by flow cytometry. Cells were cryopreserved in serum-free Cryostor CS10 at a controlled rate.

Astrocytes Mature (iPSC from Blood Cells; Male)
ASE-9322M 1 vial (1 x 10^6)
EUR 546
Description: 12 month

Astrocytes Mature (iPSC from Blood Cells; Female)
ASE-9322MF 1 vial (1 x 10^6)
EUR 546
Description: 12 month

Astrocytes Precursors (iPSC from Blood Cells; Male)
ASE-9322P 1 vial (1 x 10^6)
EUR 448.8
Description: 12 month

Astrocytes Precursors (iPSC from Blood Cells; Female)
ASE-9322PF 1 vial (1 x 10^6)
EUR 448.8
Description: 12 month

Dopaminergic Neurons (iPSC from Blood Cells; Male)
ASE-9323 1 vial
EUR 448.8
Description: 12 month

Dopaminergic Neurons (iPSC from Blood Cells; Female)
ASE-9323F 1 vial
EUR 448.8
Description: 12 month

Normal Human Peripheral Blood Mononuclear Cells, Frozen
79059-1 30 million cells
EUR 220
Description: Cryopreserved vial (30 x 10^6 cells) of freshly isolated primary human peripheral blood mononuclear cells (PBMCs) from a healthy donor, isolated from leukapheresis / apheresis samples using Ficoll gradient. After isolation, the PBMCs were stained to identify sub populations and evaluated for viability by flow cytometry. Cells were cryopreserved in serum-free Cryostor CS10 at a controlled rate.

Neurons Starter Kit (iPSC from Blood Cells; Male)
ASE-9321K 1 Kit
EUR 473.1
Description: 12 month

Neurons Starter Kit (iPSC from Blood Cells; Female)
ASE-9321KF 1 Kit
EUR 473.1
Description: 12 month

Mouse Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells
2-82072 1 Kit Ask for price

Rat Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells
2-82571 1 Kit Ask for price

Human Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells
2-96088 1 Kit Ask for price

Astrocytes Precursors Starter Kit (iPSC from Blood Cells; Male)
ASE-9322PK 1 Kit
EUR 497.4
Description: 12 month

Astrocytes Precursors Starter Kit (iPSC from Blood Cells; Female)
ASE-9322PKF 1 Kit
EUR 497.4
Description: 12 month

Dopaminergic Neurons Starter Kit (iPSC from Blood Cells; Male)
ASE-9323K 1 vial
EUR 546
Description: 12 month

Dopaminergic Neurons Starter Kit (iPSC from Blood Cells; Female)
ASE-9323KF 1 vial
EUR 546
Description: 12 month

Lysis-EZ L, White Blood Cell Lysis Buffer, 1X Strength Solution
PL015 10mL
EUR 70.44

1mL Serological Pipette Bulk Packed
170371N PK1000
EUR 151.62

2mL Serological Pipette Bulk Packed
170372N PK500
EUR 75.24

5mL Serological Pipette Bulk Packed
170373N PK200
EUR 127.68

10mL Serological Pipette Bulk Packed
170374N PK200
EUR 152.76

25mL Serological Pipette Bulk Packed
170375N PK200
EUR 116.28

50mL Serological Pipette Bulk Packed
170376N PK100
EUR 166.44

Sterilpack D200ST Sterile Individually Packed
F161330 PK400
EUR 75.24

Sterilpack D1000ST Sterile Individually Packed
F161340 PK400
EUR 83.22

Rat Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells Growth Medium
9-25071 5 x 100 ml Ask for price

Mouse Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells Growth Medium
9-32072 5 x 100 ml Ask for price

Human Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells Growth Medium
9-46088 5 x 100 ml Ask for price

white plates
1270-20 each
EUR 117.6

Human Lymph Tissue Preparation Buffer 2: Normal Lymphatic Blood Vessel Endothelial Cells
9-80088 1 x 100 ml Ask for price

Mouse Lymph Tissue Preparation Buffer 2: Normal Lymphatic Blood Vessel Endothelial Cells
9-80186 1 x 100 ml Ask for price

Rat Lymph Tissue Preparation Buffer 2: Normal Lymphatic Blood Vessel Endothelial Cells
9-80278 1 x 100 ml Ask for price

iPSC (iPSC from Blood Cells; Male, Master Lines for Neural Differentiation) *Academic Price
ASE-9109 1 vial (1 x 10^6)
EUR 1882.5
Description: 12 month

iPSC (iPSC from Blood Cells; Feale, Master Lines for Neural Differentiation) *Academic Price
ASE-9110 1 vial (1 x 10^6)
EUR 1882.5
Description: 12 month

Lysozyme (Egg White)
abx082183-5g 5 g
EUR 226.8

Lysozyme (Egg White)
abx082564-5g 5 g
EUR 243.6

Lysozyme, Egg White
L2100-005 5g
EUR 208.8

Lysozyme, Egg White
L2100-010 10g
EUR 262.8

Lysozyme, Egg White
L2100-100 100g
EUR 699.6

Safe-White™
G108-W 1.0 ml
EUR 85.2

Labeling Tape, White
TP003W 1ROLLS, 1 UNIT
EUR 64.42

Nunc White Polysorp
463200 PK60
EUR 693.12

Nunc White Maxisorp
463201 PK60
EUR 730.74

Simport Capinsert White
MIC2709 PK500
EUR 23.71
Paraffin Wax Dispenser
Paraffin wax, granular (56 - 60)
Paraffin wax, granular (56 - 60)
MagSi-WAX
MagSi-WAX
MagSi-WAX
Wax Paper For Se235 Pkg100
neo WAX 30mg Plate SPE Columns
neo WAX 100mg/3mL SPE Columns
neo WAX 200mg/6mL SPE Columns

Optimization of Viable Glioblastoma Cryopreservation for Establishment of Primary Tumor Cell Cultures

 

Background: Technologies related to the establishment of primary tumor cell cultures from solid tumors, including glioblastoma, are increasingly important to oncology research and practice. However, processing of fresh tumor specimens for establishment of primary cultures on the day of surgical collection is logistically difficult. The feasibility of viable cryopreservation of glioblastoma specimens, allowing for primary culture establishment weeks to months after surgical tumor collection and freezing, was demonstrated by Mullins et al. in 2013, with a success rate of 59% that was not significantly lower than that achieved with fresh tumor tissue. However, research targeting optimization of viable glioblastoma cryopreservation protocols for establishment of primary tumor cultures has been limited.
Objectives: The objective of this study was to optimize glioblastoma cryopreservation methods for viable cryobanking and to determine if two-dimensional (2D) or three-dimensional (3D) culture conditions were more supportive of glioblastoma growth after thawing of frozen tumor specimens.
Methods: Portions of eight human glioblastoma specimens were cryopreserved by four different protocols differing in the time of enzymatic digestion (before or after cryopreservation), and in the type of cryopreservation media (CryoStor CS10 or 10% dimethyl sulfoxide and 90% fetal calf serum). After 1 month, frozen tissues were thawed, enzymatically digested, if not digested before, and used for initiation of 2D or 3D primary tumor cultures to determine viability.
Results: Among the tested cryopreservation and culturing protocols, the most efficient combinations of cryopreservation and culture were those associated with the use of CryoStor CS10 cryopreservation medium, enzymatic digestion before freezing, and 2D culturing after thawing with a successful culture rate of 8 out of 8 cases (100%). Two-dimensional cultures were in general more efficient for the support of tumor cell growth after thawing than 3D cultures.

Metformin partially reverses the inhibitory effect of co-culture with ER-/PR-/HER2+ breast cancer cells on biomarkers of monocyte antitumor activity

Background: Immune activities of monocytes (MOs) can be altered within the microenvironment of solid malignancies, including breast cancer. Metformin (1,1-dimethylbiguanide hydrochloride, MET), has been shown to decrease tumor cell proliferation, but its effects have yet to be explored with respect to MOs (monocytes) activity during their crosstalk with breast cancer cells. Here, we investigated the effects of MET on overall phenotypic functional activities, including cellular immunometabolism and protective redox signaling based-biomarkers, intracellular free calcium ions (ifCa2+), phagocytosis and co-operative cytokines (IFN-γ and IL-10) of autologous MOs before and during their interplay with primary ER-/PR-/HER2+ breast cancer cells.
Methods: Human primary breast cancer cells were either cultured alone or co-cultured with autologous MOs before treatment with MET.
Results: MET downregulated breast cancer cell proliferation and phagocytosis, while having no significant effect on the ratio of phosphorylated Akt (p-Akt) to total Akt. Additionally, we observed that, in the absence of MET treatment, the levels of lactate dehydrogenase (LDH)-based cytotoxicity, catalase, ifCa2+, IL-10 and arginase activity were significantly reduced in co-cultures compared to levels in MOs cultured alone whereas levels of inducible nitric oxide synthase (iNOS) activity were significantly increased. In contrast, MET treatment reduced the effects measured in co-culture on the levels of LDH-based cytotoxicity, arginase activity, catalase, ifCa2+, and IFN-γ. MET also induced upregulation of both iNOS and arginase in MO cells, although the increase did not reach significant difference for iNOS activity.
Moreover, MET induced a robust increase of superoxide dismutase (SOD) activity in MOs, but not in MOs co-cultured with breast cancer cells. Furthermore, MET markedly upregulated the levels of IFN-γ production and downregulated those of IL-10 in isolated MOs, while inducing a slight opposing up-regulation of IL-10 production in co-cultures.
MagIso CD8 Memory T Cell Isolation Kit, Human
WHK-B026 20 tests, 200 tests Ask for price
CD45RO Antibody (T-cell marker)
V2419IHC-7ML 7 ml
EUR 499
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2419SAF-100UG 100 ug
EUR 499
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2420-100UG 100 ug
EUR 499
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2420-20UG 20 ug
EUR 219
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2420IHC-7ML 7 ml
EUR 499
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2420SAF-100UG 100 ug
EUR 499
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V7044-100UG 100 ug
EUR 499
Description: CD45RO is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. CD45RO contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. It has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. It also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. [RefSeq]
CD45RO Antibody (T-cell marker)
V7044-20UG 20 ug
EUR 219
Description: CD45RO is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. CD45RO contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. It has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. It also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. [RefSeq]
CD45RO Antibody (T-cell marker)
V7044IHC-7ML 7 ml
EUR 499
Description: CD45RO is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. CD45RO contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. It has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. It also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. [RefSeq]
CD45RO Antibody (T-cell marker)
V7044SAF-100UG 100 ug
EUR 499
Description: CD45RO is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. CD45RO contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. It has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. It also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. [RefSeq]
CD45RO Antibody (T-cell marker)
V2237-100UG 100 ug
EUR 499
Description: This antibody recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2237-20UG 20 ug
EUR 219
Description: This antibody recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2237IHC-7ML 7 ml
EUR 499
Description: This antibody recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2237SAF-100UG 100 ug
EUR 499
Description: This antibody recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2416-100UG 100 ug
EUR 499
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2416-20UG 20 ug
EUR 219
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2416SAF-100UG 100 ug
EUR 499
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2419-100UG 100 ug
EUR 499
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2419-20UG 20 ug
EUR 219
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-10 each Ask for price
Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-20 each Ask for price
Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-30 each Ask for price
Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-40 each Ask for price
Untransduced T Cells
78170-1 1 vial
EUR 1250
Description: The untransduced T cells are produced by mock lentiviral transduction of human primary CD4+CD8+ T cells. These cells are subjected to comparable manipulations as CAR-T cells: activation, spinoculation (without lentivirus), and expansion. These T cells are meant to be negative controls in experiments using lentivirus-transduced primary CAR-T cells.
Untransduced T Cells
78170-2 5 vials
EUR 3325
Description: The untransduced T cells are produced by mock lentiviral transduction of human primary CD4+CD8+ T cells. These cells are subjected to comparable manipulations as CAR-T cells: activation, spinoculation (without lentivirus), and expansion. These T cells are meant to be negative controls in experiments using lentivirus-transduced primary CAR-T cells.
CD45RO, T-Cell; Clone UCHL1 Concentrate
A00024-C 1 ml
EUR 600
CD45RO Antibody Cocktail (T-cell marker)
V2421-100UG 100 ug
EUR 499
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody cocktail reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody Cocktail (T-cell marker)
V2421-20UG 20 ug
EUR 219
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody cocktail reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody Cocktail (T-cell marker)
V2421IHC-7ML 7 ml
EUR 499
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody cocktail reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody Cocktail (T-cell marker)
V2421SAF-100UG 100 ug
EUR 499
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody cocktail reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx159398
  • EUR 326.40
  • EUR 276.00
  • 100 ug
  • 50 ug
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159399-100ul 100 ul
EUR 427.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159400-100ul 100 ul
EUR 427.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159401-100ul 100 ul
EUR 427.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159402-100ul 100 ul
EUR 427.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159403-100ul 100 ul
EUR 526.8
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159404-100ul 100 ul
EUR 427.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159405-100ul 100 ul
EUR 427.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159406-100ul 100 ul
EUR 427.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx137099
  • EUR 427.20
  • EUR 627.60
  • 0.5 mg
  • 1 mg
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx139124-01mg 0.1 mg
EUR 427.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx139125-01mg 0.1 mg
EUR 427.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx139126-01mg 0.1 mg
EUR 427.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx139127-01mg 0.1 mg
EUR 427.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx140007-01mg 0.1 mg
EUR 343.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx140008-01mg 0.1 mg
EUR 343.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx140009-01mg 0.1 mg
EUR 343.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx140102-01mg 0.1 mg
EUR 376.8
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx140341-01mg 0.1 mg
EUR 427.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx140432-01mg 0.1 mg
EUR 427.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx134635
  • EUR 427.20
  • EUR 678.00
  • EUR 360.00
  • 100 ul
  • 200 ul
  • 50 ul
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx134645
  • EUR 427.20
  • EUR 678.00
  • EUR 360.00
  • 100 ul
  • 200 ul
  • 50 ul
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx134646
  • EUR 427.20
  • EUR 678.00
  • EUR 360.00
  • 100 ul
  • 200 ul
  • 50 ul
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx146722-100ug 100 ug
EUR 526.8
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx146728-100ug 100 ug
EUR 526.8
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx000694
  • EUR 493.20
  • EUR 710.40
  • 100 ul
  • 200 ul
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx001304
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx009476
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx125313
  • EUR 594.00
  • EUR 844.80
  • EUR 427.20
  • 100 ul
  • 200 ul
  • 50 ul
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx111482
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx012383-100ul 100 ul
EUR 493.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx015800-100ul 100 ul
EUR 493.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx019049-100ug 100 ug
EUR 410.4
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx021410-02mg 0.2 mg
EUR 886.8
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx028455-400ul 400 ul
EUR 627.6
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx028455-80l 80 µl
EUR 343.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx028456-400ul 400 ul
EUR 661.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx028456-80l 80 µl
EUR 385.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx200030-100ug 100 ug
EUR 393.6
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx200854
  • EUR 326.40
  • EUR 493.20
  • 100 ug
  • 500 ug
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx320336
  • EUR 360.00
  • EUR 292.80
  • 100 ul
  • 50 ul
T-Cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx242941
  • EUR 560.40
  • EUR 393.60
  • 100 ul
  • 50 ul
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx326208
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413138-01mg 0.1 mg
EUR 526.8
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413139-025mg 0.25 mg
EUR 678
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413254-01mg 0.1 mg
EUR 543.6
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413340-025mg 0.25 mg
EUR 678
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413341-05mg 0.5 mg
EUR 994.8
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413342-01mg 0.1 mg
EUR 526.8
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413343-25ug 25 ug
EUR 326.4
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413612-2ml 2 ml
EUR 727.2
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413766-01mg 0.1 mg
EUR 610.8
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413842-01mg 0.1 mg
EUR 543.6
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414255-025mg 0.25 mg
EUR 710.4
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414488-025mg 0.25 mg
EUR 678
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414492-25ug 25 ug
EUR 326.4
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414713-025mg 0.25 mg
EUR 678
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414714-1mg 1 mg
EUR 994.8
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414765-025mg 0.25 mg
EUR 678
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