Optimization of Viable Glioblastoma Cryopreservation for Establishment of Primary Tumor Cell Cultures

November 12, 2020
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eaaci2015

Development of new media formulations for cell culture operations based on regression models

The paper discusses modelling and optimization of multi-component cell culture medium. The specific productivity (Qp) was considered a function of the medium components and possible interactions described by linear factors, two-way interactions and squared terms that results in a high dimensional problem where the number of variables p (represented by the medium components and their interactions) is much larger than the number of observations n.
Principal Components Regression (PCR), Partial Least Squares (PLS), Lasso and Elastic Net regressions were compared as modelling tools to deal with a high dimensional [Formula: see text] problem. PCR and PLS regression models resulted in better prediction results and were used for robust optimization of the medium composition by a nonlinear optimization. The case studies show that it is possible to formulate new media that result in higher Qp than the ones provided by the initial media experiments available. Also, the multivariate statistical approach permitted us to select media that is most informative about the optimum thus permitting modelling and optimization with a reduced set of initial experiments.

Heat Stress Triggers Differential Protein Accumulation in the Extracellular Matrix of Sorghum Cell Suspension Cultures

Plants reprogram gene expression as an adaptive response to survive high temperatures. While the identity and functions of intracellular heat stress-responsive proteins have been extensively studied, the heat response of proteins secreted to the extracellular matrix is unknown. Here, we used Sorghum bicolor, a species adapted for growth in hot climates, to investigate the extracellular heat-induced responses. When exposed to 40 C for 72 h, heat-sensitive Arabidopsis cell suspension cultures died, while ICSB338 sorghum cell cultures survived by activation of a transcriptional response characterized by the induction of Gentaur.
Quantitative proteomic analysis of proteins recovered from cell culture medium revealed specific heat stress-induced protein accumulation within the sorghum secretome. Of the 265 secreted proteins identified, 31 responded to heat (2-fold change), with 84% possessing a predicted signal peptide for targeting to the classical secretory pathway. The differentially accumulated proteins have putative functions in metabolism, detoxification, and protein modifications. A germin (SORBI_3003G427700) was highly heat-inducible at both protein and gene level. Overall, our study reveals new insights into sorghum responses to heat and provides a useful resource of extracellular proteins that could serve as targets for developing thermotolerant crops. Data are available via ProteomeXchange with identifier PXD021536.

Xenograft and cell culture models of Sézary syndrome reveal cell of origin diversity and subclonal heterogeneity

Sézary Syndrome (SS) is a rare aggressive epidermotropic cutaneous T-cell lymphoma (CTCL) defined by erythroderma, pruritis, and a circulating atypical CD4 + T-cell clonal population. The diversity of Sézary cell (SC) phenotype and genotype may reflect either plasticity or heterogeneity, which was difficult to evaluate dynamically until the achievement of long-term SC expansion. Therefore, we developed six defined culture conditions allowing for the expansion of SC defined by their phenotype and monoclonality in four of seven SS cases. Engraftment of SC through the intrafemoral route into immunodeficient NOD.Cg-Prkdc(scid)Il2rg(tm1Wjll)/SzJ (NSG) mice was achieved in 2 of 14 SS cases. Secondary xenograft by percutaneous injection mimicked most of the features of SS with dermal infiltration, epidermotropism, and blood spreading.
These models also allowed assessing the intra-individual heterogeneity of patient SC. Subclones sharing the same TCR gene rearrangement evolved independently according to culture conditions and/or after xenografting. This clonal selection was associated with some immunophenotypic plasticity and limited genomic evolution both in vitro and in vivo. The long-term amplification of SC allowed us to develop eight new SC lines derived from four different patients. These lines represent the cell of origin diversity of SC and provide new tools to evaluate their functional hallmarks and response to therapy.
eaaci2015

eaaci2015

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Optimization of Viable Glioblastoma Cryopreservation for Establishment of Primary Tumor Cell Cultures

 

Background: Technologies related to the establishment of primary tumor cell cultures from solid tumors, including glioblastoma, are increasingly important to oncology research and practice. However, processing of fresh tumor specimens for establishment of primary cultures on the day of surgical collection is logistically difficult. The feasibility of viable cryopreservation of glioblastoma specimens, allowing for primary culture establishment weeks to months after surgical tumor collection and freezing, was demonstrated by Mullins et al. in 2013, with a success rate of 59% that was not significantly lower than that achieved with fresh tumor tissue. However, research targeting optimization of viable glioblastoma cryopreservation protocols for establishment of primary tumor cultures has been limited.
Objectives: The objective of this study was to optimize glioblastoma cryopreservation methods for viable cryobanking and to determine if two-dimensional (2D) or three-dimensional (3D) culture conditions were more supportive of glioblastoma growth after thawing of frozen tumor specimens.
Methods: Portions of eight human glioblastoma specimens were cryopreserved by four different protocols differing in the time of enzymatic digestion (before or after cryopreservation), and in the type of cryopreservation media (CryoStor CS10 or 10% dimethyl sulfoxide and 90% fetal calf serum). After 1 month, frozen tissues were thawed, enzymatically digested, if not digested before, and used for initiation of 2D or 3D primary tumor cultures to determine viability.
Results: Among the tested cryopreservation and culturing protocols, the most efficient combinations of cryopreservation and culture were those associated with the use of CryoStor CS10 cryopreservation medium, enzymatic digestion before freezing, and 2D culturing after thawing with a successful culture rate of 8 out of 8 cases (100%). Two-dimensional cultures were in general more efficient for the support of tumor cell growth after thawing than 3D cultures.

Metformin partially reverses the inhibitory effect of co-culture with ER-/PR-/HER2+ breast cancer cells on biomarkers of monocyte antitumor activity

Background: Immune activities of monocytes (MOs) can be altered within the microenvironment of solid malignancies, including breast cancer. Metformin (1,1-dimethylbiguanide hydrochloride, MET), has been shown to decrease tumor cell proliferation, but its effects have yet to be explored with respect to MOs (monocytes) activity during their crosstalk with breast cancer cells. Here, we investigated the effects of MET on overall phenotypic functional activities, including cellular immunometabolism and protective redox signaling based-biomarkers, intracellular free calcium ions (ifCa2+), phagocytosis and co-operative cytokines (IFN-γ and IL-10) of autologous MOs before and during their interplay with primary ER-/PR-/HER2+ breast cancer cells.
Methods: Human primary breast cancer cells were either cultured alone or co-cultured with autologous MOs before treatment with MET.
Results: MET downregulated breast cancer cell proliferation and phagocytosis, while having no significant effect on the ratio of phosphorylated Akt (p-Akt) to total Akt. Additionally, we observed that, in the absence of MET treatment, the levels of lactate dehydrogenase (LDH)-based cytotoxicity, catalase, ifCa2+, IL-10 and arginase activity were significantly reduced in co-cultures compared to levels in MOs cultured alone whereas levels of inducible nitric oxide synthase (iNOS) activity were significantly increased. In contrast, MET treatment reduced the effects measured in co-culture on the levels of LDH-based cytotoxicity, arginase activity, catalase, ifCa2+, and IFN-γ. MET also induced upregulation of both iNOS and arginase in MO cells, although the increase did not reach significant difference for iNOS activity.
Moreover, MET induced a robust increase of superoxide dismutase (SOD) activity in MOs, but not in MOs co-cultured with breast cancer cells. Furthermore, MET markedly upregulated the levels of IFN-γ production and downregulated those of IL-10 in isolated MOs, while inducing a slight opposing up-regulation of IL-10 production in co-cultures.
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Human  Peripheral  Blood  CD14+ Monocytes, Fresh / Positive selection
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Human  Peripheral  Blood  CD14+ Monocytes, Cryopreserved / Positive selection
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CD19 Positive Cell Isolation Kit
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CD19 Positive Cell Isolation Kit
78564-2 1 x 10^9 Cells
EUR 630
Description: The CD19 Positive Cell Isolation Kit is designed to magnetically separate CD19-expressing-cells from a complex immune cell population. This kit is optimized for the isolation of CD19 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, CD19-positive cells will be immobilized along the side of the tube while undesired CD19-negative cells will remain in suspension for easy removal.
4GB USB memory stick
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ICP-MS Memory Test 1
CL-MEM-1 125ML
EUR 483.6
ICP-MS Memory Test 2
CL-MEM-2 125ML
EUR 273.6
CD45RA/CD45RO/CD3/CD4
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Human Cord Blood CD4+/ CD45RA+ Naive T Cells
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ICP-MS Set of 2 Memory Test Solutions
CL-MEM-SET EACH
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Kinesis Rheodyne TitanHP 6 Position 7 Port Selection Valve;Ti;PCB
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VERO Host Cell Proteins (HCPs) positive control (10 ug/ml)
770-165-PC1 1 ml
EUR 270
Human Normal Peripheral Blood CD4+/CD45RA+/CD25- Naive T Cells
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EUR 1090.8
Human Normal Peripheral Blood CD4+/CD45RA+/CD25- Naive T Cells
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Single Donor Human SARS-CoV-2 Positive With Vaccine Positive Serum
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Single Donor Human SARS-CoV-2 Positive With Vaccine Positive Plasma
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EUR 209
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CD45RO Antibody (T-cell marker)
V7044-100UG 100 ug
EUR 349.3
Description: CD45RO is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. CD45RO contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. It has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. It also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. [RefSeq]
CD45RO Antibody (T-cell marker)
V7044-20UG 20 ug
EUR 153.3
Description: CD45RO is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. CD45RO contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. It has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. It also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. [RefSeq]
CD45RO Antibody (T-cell marker)
V7044IHC-7ML 7 ml
EUR 349.3
Description: CD45RO is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. CD45RO contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. It has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. It also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. [RefSeq]
CD45RO Antibody (T-cell marker)
V7044SAF-100UG 100 ug
EUR 349.3
Description: CD45RO is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. CD45RO contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. It has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. It also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. [RefSeq]
CD45RO Antibody (T-cell marker)
V2416-100UG 100 ug
EUR 349.3
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2416-20UG 20 ug
EUR 153.3
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2416SAF-100UG 100 ug
EUR 349.3
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2419-100UG 100 ug
EUR 349.3
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2419-20UG 20 ug
EUR 153.3
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2419IHC-7ML 7 ml
EUR 349.3
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2419SAF-100UG 100 ug
EUR 349.3
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2420-100UG 100 ug
EUR 349.3
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2420-20UG 20 ug
EUR 153.3
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2420IHC-7ML 7 ml
EUR 349.3
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2420SAF-100UG 100 ug
EUR 349.3
Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2237-100UG 100 ug
EUR 349.3
Description: This antibody recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2237-20UG 20 ug
EUR 153.3
Description: This antibody recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2237IHC-7ML 7 ml
EUR 349.3
Description: This antibody recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CD45RO Antibody (T-cell marker)
V2237SAF-100UG 100 ug
EUR 349.3
Description: This antibody recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Human Complement C3d AssayMax ELISA Kit (Positive Control included)
EC3331-7 96 Well Plate
EUR 572.4
RSV Positive Sample
DAG-H10378 1 ml dry ice
EUR 1326
HEK293 Host Cell Proteins (HEK293-HCP) positive control (10 ug/ml)
740-150-PC1 1 ml
EUR 270
ROS1 positive non-small cell lung NSCLC cancer tissue array
LC261 each
EUR 360
Description: ROS1 positive non-small cell lung NSCLC cancer tissue array, including pathology grade, TNM, clinical stage, IHC ROS1+ results (FISH on request), 21 cases/26 cores
Human Serum (AB positive)
90-1011 100 ml
EUR 580.8
Description: Pooled Normal Human Serum (AB positive)
Pichia Pastoris Host Cell Proteins (PP-HCP) positive control (10 ug/ml)
750-155-PC1 1 ml
EUR 270
Human Cell-30 protein lysate for WB positive control for PR12-M r protein (PR) IgG #2
PR12-C 100 ul
EUR 343.2
QPCR Positive Control Kit
M1126-100 each
EUR 483.6
Chinese Hamster Ovary Cell (CHO) host cell Proteins (HCPs) positive control (10 ug/ml)
800-140-PC1 1 ml
EUR 270
Human Cell-18 protein lysate for WB positive control for ERA12-A protein (PR) IgG #2
ERA12-C 100 ul
EUR 343.2
NATtrol EV Positive Control (6 X 0.2 mL)
NATEVPOS-6MC 6 X 0.2 mL
EUR 208
NATtrol SA Positive Control (6 X 0.5 mL)
NATMSSA-6MC 6 X 0.5 mL
EUR 311
NATtrol SA Positive Control  (6 X 0.5 mL)
NATMSSA-6MC-IVD 6 X 0.5 mL
EUR 331
NATtrol EV Positive Control (6 X 0.5 mL)
MDZ055 6 X 0.2 mL
EUR 208
NATtrol SA Positive Control (6 X 0.5 mL)
MDZ061 6 X 0.5 mL
EUR 378.24
Description: Please contact Gentaur in order to receive the datasheet of the product.
Rat Positive Cofactor 4 (PC4) CLIA Kit
abx197523-96tests 96 tests
EUR 990
Rotavirus Positive Sample
DAG-H10377 1 ml dry ice
EUR 1326
Dog Positive Cofactor 4 ELISA kit
E08P0063-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine Positive Cofactor 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Positive Cofactor 4 ELISA kit
E08P0063-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine Positive Cofactor 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Positive Cofactor 4 ELISA kit
E08P0063-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine Positive Cofactor 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
NATtrol HSV 1 & 2 Positive Control (6 x 0.25 mL)
NATHSV-6L 6 x 0.25 mL
EUR 272
NATtrol RSV Positive Control (6 X 0.5 mL)
NATRSV-6C-IVD 6 X 0.5 mL
EUR 242
NATtrol GBS Positive Control (6 x 0.25 mL)
NATSAG-6L 6 x 0.25 mL
EUR 272
NATtrol GBS Positive Control (6 X 0.5 mL)
NATSAG-6MC 6 X 0.5 mL
EUR 313
NATtrol GBS Positive Control (6 x 0.5 mL)
NATSAG-6MC-IVD 6 x 0.5 mL
EUR 333
NATtrol CT/NG/TV Positive Control (6 x 1.0mL)
NATCTNGTV-POS 6 x 1.0mL
EUR 368
Rat Positive Cofactor 4 ELISA kit
E02P0063-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat Positive Cofactor 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Positive Cofactor 4 ELISA kit
E02P0063-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat Positive Cofactor 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Positive Cofactor 4 ELISA kit
E02P0063-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat Positive Cofactor 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
NATtrol RSV Positive Control (6 X 0.5 mL)
MDZ047 6 X 0.5 mL
EUR 278.4
Description: Please contact Gentaur in order to receive the datasheet of the product.
NATtrol GBS Positive Control (6 x 0.5 mL)
MDZ053 6 x 0.5 mL
EUR 378.24
Description: Please contact Gentaur in order to receive the datasheet of the product.
Pig Positive Cofactor 4 ELISA kit
E07P0063-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine Positive Cofactor 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Positive Cofactor 4 ELISA kit
E07P0063-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Porcine Positive Cofactor 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Positive Cofactor 4 ELISA kit
E07P0063-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine Positive Cofactor 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Positive Cofactor 4 (PC4) ELISA Kit
abx255897-96tests 96 tests
EUR 848.4
Pig Positive Cofactor 4 (PC4) ELISA Kit
abx361959-96tests 96 tests
EUR 990
CLIA kit for Rat PC4 (Positive Cofactor 4)
E-CL-R0542 1 plate of 96 wells
EUR 700.8
Description: A sandwich CLIA kit for quantitative measurement of Rat PC4 (Positive Cofactor 4) in samples from Serum, Plasma, Cell supernatant
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