Optimization of Viable Glioblastoma Cryopreservation for Establishment of Primary Tumor Cell Cultures

eaaci2015

Development of new media formulations for cell culture operations based on regression models

The paper discusses modelling and optimization of multi-component cell culture medium. The specific productivity (Qp) was considered a function of the medium components and possible interactions described by linear factors, two-way interactions and squared terms that results in a high dimensional problem where the number of variables p (represented by the medium components and their interactions) is much larger than the number of observations n.
Principal Components Regression (PCR), Partial Least Squares (PLS), Lasso and Elastic Net regressions were compared as modelling tools to deal with a high dimensional [Formula: see text] problem. PCR and PLS regression models resulted in better prediction results and were used for robust optimization of the medium composition by a nonlinear optimization. The case studies show that it is possible to formulate new media that result in higher Qp than the ones provided by the initial media experiments available. Also, the multivariate statistical approach permitted us to select media that is most informative about the optimum thus permitting modelling and optimization with a reduced set of initial experiments.

Heat Stress Triggers Differential Protein Accumulation in the Extracellular Matrix of Sorghum Cell Suspension Cultures

Plants reprogram gene expression as an adaptive response to survive high temperatures. While the identity and functions of intracellular heat stress-responsive proteins have been extensively studied, the heat response of proteins secreted to the extracellular matrix is unknown. Here, we used Sorghum bicolor, a species adapted for growth in hot climates, to investigate the extracellular heat-induced responses. When exposed to 40 C for 72 h, heat-sensitive Arabidopsis cell suspension cultures died, while ICSB338 sorghum cell cultures survived by activation of a transcriptional response characterized by the induction of HSP70 and HSP90 genes.
Quantitative proteomic analysis of proteins recovered from cell culture medium revealed specific heat stress-induced protein accumulation within the sorghum secretome. Of the 265 secreted proteins identified, 31 responded to heat (2-fold change), with 84% possessing a predicted signal peptide for targeting to the classical secretory pathway. The differentially accumulated proteins have putative functions in metabolism, detoxification, and protein modifications. A germin (SORBI_3003G427700) was highly heat-inducible at both protein and gene level. Overall, our study reveals new insights into sorghum responses to heat and provides a useful resource of extracellular proteins that could serve as targets for developing thermotolerant crops. Data are available via ProteomeXchange with identifier PXD021536.

Xenograft and cell culture models of Sézary syndrome reveal cell of origin diversity and subclonal heterogeneity

Sézary Syndrome (SS) is a rare aggressive epidermotropic cutaneous T-cell lymphoma (CTCL) defined by erythroderma, pruritis, and a circulating atypical CD4 + T-cell clonal population. The diversity of Sézary cell (SC) phenotype and genotype may reflect either plasticity or heterogeneity, which was difficult to evaluate dynamically until the achievement of long-term SC expansion. Therefore, we developed six defined culture conditions allowing for the expansion of SC defined by their phenotype and monoclonality in four of seven SS cases. Engraftment of SC through the intrafemoral route into immunodeficient NOD.Cg-Prkdc(scid)Il2rg(tm1Wjll)/SzJ (NSG) mice was achieved in 2 of 14 SS cases. Secondary xenograft by percutaneous injection mimicked most of the features of SS with dermal infiltration, epidermotropism, and blood spreading.
These models also allowed assessing the intra-individual heterogeneity of patient SC. Subclones sharing the same TCR gene rearrangement evolved independently according to culture conditions and/or after xenografting. This clonal selection was associated with some immunophenotypic plasticity and limited genomic evolution both in vitro and in vivo. The long-term amplification of SC allowed us to develop eight new SC lines derived from four different patients. These lines represent the cell of origin diversity of SC and provide new tools to evaluate their functional hallmarks and response to therapy.
eaaci2015

eaaci2015

Dog Red Blood Cells
88R-D004 10 ml
EUR 273.00
Description: Glutaraldehyde-stabilized freshly prepared Dog Red Blood Cells
Goat Red Blood Cells
88R-G003 20 ml
EUR 273.00
Description: Glutaraldehyde-stabilized freshly prepared Goat Red Blood Cells
Hamster Red Blood Cells
88R-H001 5 ml
EUR 273.00
Description: Glutaraldehyde-stabilized freshly prepared Hamster Red Blood Cells
Horse Red Blood Cells
88R-H002 20 ml
EUR 273.00
Description: Glutaraldehyde-stabilized freshly prepared Horse Red Blood Cells
Hamster Red Blood Cells
88R-H003 10 ml
EUR 489.00
Description: Washed and freshly prepared 10% suspension of Hamster Red Blood Cells
Mouse Red Blood Cells
88R-M001 10 ml
EUR 295.00
Description: Washed and freshly prepared 10% suspension of Mouse Red Blood Cells
Monkey Red Blood Cells
88R-M003 5 x 2 ml
EUR 273.00
Description: Glutaraldehyde-stabilized freshly prepared Monkey Red Blood Cells
Mouse Red Blood Cells
88R-M004 5 x 2 ml
EUR 273.00
Description: Glutaraldehyde-stabilized freshly prepared Mouse Red Blood Cells
Pig Red Blood Cells
88R-P002 20 ml
EUR 273.00
Description: Glutaraldehyde-stabilized freshly prepared Pig Red Blood Cells
Rabbit Red Blood Cells
88R-R001 20 ml
EUR 273.00
Description: Glutaraldehyde-stabilized freshly prepared Rabbit Red Blood Cells
Rat Red Blood Cells
88R-R002 5 x 2.0ml
EUR 273.00
Description: Glutaraldehyde-stabilized freshly prepared Rat Red Blood Cells
Rat Red Blood Cells
88R-R005 25 ml
EUR 327.00
Description: Washed and freshly prepared 10% suspension of Rat Red Blood Cells
Sheep Red Blood Cells
88R-S001 100 ml
EUR 512.00
Description: Washed and freshly prepared 10% suspension of Sheep Red Blood Cells
Sheep Red Blood Cells
88R-S003 20 ml
EUR 273.00
Description: Glutaraldehyde-stabilized freshly prepared Sheep Red Blood Cells
Sheep Red Blood Cells
88R-S004 10 ml
EUR 521.00
Description: Glutaraldehyde-stabilized freshly prepared albumin sensitised Sheep Red Blood Cells
Sheep Red Blood Cells
88R-S005 10 ml
EUR 446.00
Description: Glutaraldehyde-stabilized freshly prepared tanned Sheep Red Blood Cells
Turkey Red Blood Cells
88R-T001 20 ml
EUR 273.00
Description: Glutaraldehyde-stabilized freshly prepared Turkey Red Blood Cells
Guinea Pig Red Blood Cells
88R-1047 15 ml
EUR 273.00
Description: Glutaraldehyde-stabilized freshly prepared Guinea Pig Red Blood Cells
Guinea Pig Red Blood Cells
88R-G002 25 ml
EUR 365.00
Description: Washed and freshly prepared 10% suspension of Guinea Pig Red Blood Cells
Guinea Pig Red Blood Cells
88R-P001 15 ml
EUR 273.00
Description: Glutaraldehyde-stabilized freshly prepared Guinea Pig Red Blood Cells
Rabbit Red Blood Cells (100%)
20-abx800015
  • EUR 648.00
  • EUR 509.00
  • 0
  • 1
  • Shipped within 1-5 working days.
Sheep Red Blood Cells (100%)
20-abx800032
  • EUR 356.00
  • EUR 314.00
  • 0
  • 1
  • Shipped within 1-5 working days.
Goat Red Blood Cells (100%)
20-abx800047
  • EUR 384.00
  • EUR 328.00
  • 0
  • 1
  • Shipped within 1-5 working days.
Horse Red Blood Cells (100%)
20-abx800062
  • EUR 453.00
  • EUR 551.00
  • EUR 384.00
  • 0
  • 1
  • 2
  • Shipped within 1-5 working days.
Horse Red Blood Cells (100%)
abx800062-1L 1 L
EUR 746.00
  • Shipped within 1-5 working days.
Donkey Red Blood Cells (100%)
20-abx800077
  • EUR 676.00
  • EUR 843.00
  • EUR 481.00
  • 0
  • 1
  • 2
  • Shipped within 1-5 working days.
Donkey Red Blood Cells (100%)
abx800077-1L 1 L
EUR 1191.00
  • Shipped within 1-5 working days.
Camel Red Blood Cells (100%)
20-abx800092
  • EUR 746.00
  • EUR 1636.00
  • EUR 578.00
  • 0
  • 1
  • 2
  • Shipped within 1-5 working days.
Camel Red Blood Cells (100%)
abx800092-1L 1 L
EUR 3042.00
  • Shipped within 1-5 working days.
Dog Red Blood Cells (100%)
20-abx800122
  • EUR 592.00
  • EUR 481.00
  • 0
  • 1
  • Shipped within 1-5 working days.
Chicken Red Blood Cells (100%)
20-abx800137
  • EUR 384.00
  • EUR 328.00
  • 0
  • 1
  • Shipped within 1-5 working days.
Neural Stem Cells (iPSC from Blood Cells; Male)
ASE-9234 1 vial (1 x 10^6)
EUR 414.50
Description: 12 month
Neural Stem Cells (iPSC from Blood Cells; Female)
ASE-9234F 1 vial (1 x 10^6)
EUR 414.50
Description: 12 month
Human Cord Blood CD34+ Cells Derived Endothelial Cells
HEC23 500,000+ Cells
EUR 1080.00
Neurons (iPSC from Blood Cells; Male)
ASE-9321 1 vial (1 x 10^6)
EUR 313.25
Description: 12 month
Neurons (iPSC from Blood Cells; Female)
ASE-9321F 1 vial (1 x 10^6)
EUR 313.25
Description: 12 month
Beagle dog Red Blood Cells (100%)
20-abx800107
  • EUR 829.00
  • EUR 662.00
  • 0
  • 1
  • Shipped within 1-5 working days.
Astrocytes Mature (iPSC from Blood Cells; Male)
ASE-9322M 1 vial (1 x 10^6)
EUR 455.00
Description: 12 month
Astrocytes Mature (iPSC from Blood Cells; Female)
ASE-9322MF 1 vial (1 x 10^6)
EUR 455.00
Description: 12 month
Astrocytes Precursors (iPSC from Blood Cells; Male)
ASE-9322P 1 vial (1 x 10^6)
EUR 374.00
Description: 12 month
Astrocytes Precursors (iPSC from Blood Cells; Female)
ASE-9322PF 1 vial (1 x 10^6)
EUR 374.00
Description: 12 month
Dopaminergic Neurons (iPSC from Blood Cells; Male)
ASE-9323 1 vial
EUR 374.00
Description: 12 month
Dopaminergic Neurons (iPSC from Blood Cells; Female)
ASE-9323F 1 vial
EUR 374.00
Description: 12 month
Lysis-EZ L, White Blood Cell Lysis Buffer, 1X Strength Solution
PL015 10mL
EUR 58.70
  • Product category: Molecular Biology Kits/Lysis/White Blood Cells
Neurons Starter Kit (iPSC from Blood Cells; Male)
ASE-9321K 1 Kit
EUR 394.25
Description: 12 month
Neurons Starter Kit (iPSC from Blood Cells; Female)
ASE-9321KF 1 Kit
EUR 394.25
Description: 12 month
Human Cord Blood CD34+ Cells, Cryopreserved, single donor
CBCD34-C0.5M NULL
EUR NULL
Human Cord Blood CD34+ Cells, Cryopreserved, single donor 
CBCD34-C1M NULL
EUR NULL
Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor 
CBCD34mix-C0.5M NULL
EUR NULL
Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor 
CBCD34mix-C1M NULL
EUR NULL
Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor 
CBCD34mix-C5M NULL
EUR NULL
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh
BMC-F500M NULL
EUR NULL
Human Peripheral Blood CD3+ T Cells, Cryopreserved
PBCD3-C10M NULL
EUR NULL
Human Peripheral Blood CD3+ T Cells, Cryopreserved
PBCD3-C20M NULL
EUR NULL
Human Peripheral Blood CD3+ T Cells, Fresh
PBCD3-F20M NULL
EUR NULL
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved
PBMC-C100M NULL
EUR NULL
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved
PBMC-C10M NULL
EUR NULL
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved
PBMC-C20M NULL
EUR NULL
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved
PBMC-C50M NULL
EUR NULL
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh
PBMC-F100M NULL
EUR NULL
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh
PBMC-F1B NULL
EUR NULL
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh
PBMC-F2B NULL
EUR NULL
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh
PBMC-F300M NULL
EUR NULL
white plates
1270-20
EUR 98.00
Mouse Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells
2-82072 1 Kit Ask for price
Rat Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells
2-82571 1 Kit Ask for price
Human Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells
2-96088 1 Kit Ask for price
Astrocytes Precursors Starter Kit (iPSC from Blood Cells; Male)
ASE-9322PK 1 Kit
EUR 414.50
Description: 12 month
Astrocytes Precursors Starter Kit (iPSC from Blood Cells; Female)
ASE-9322PKF 1 Kit
EUR 414.50
Description: 12 month
Dopaminergic Neurons Starter Kit (iPSC from Blood Cells; Male)
ASE-9323K 1 vial
EUR 455.00
Description: 12 month
Dopaminergic Neurons Starter Kit (iPSC from Blood Cells; Female)
ASE-9323KF 1 vial
EUR 455.00
Description: 12 month
Positively Selected Normal Peripheral Blood CD19+ B Cells, Cryopreserved
PBCD19P-C10M NULL
EUR NULL
Positively Selected Normal Peripheral Blood CD19+ B Cells, Fresh
PBCD19P-F10M NULL
EUR NULL
Lysozyme (Egg White)
abx082183-5g 5 g
EUR 189.00
  • Shipped within 5-10 working days.
Lysozyme (Egg White)
abx082564-5g 5 g
EUR 203.00
  • Shipped within 5-10 working days.
Safe-White™
G108-W 1.0 ml
EUR 71.00
Lysozyme, Egg White
L2100-005 5g
EUR 174.00
Lysozyme, Egg White
L2100-010 10g
EUR 219.00
Lysozyme, Egg White
L2100-100 100g
EUR 583.00
Labeling Tape, White
TP003W 1ROLLS, 1 UNIT
EUR 53.68
  • Product category: Labware/Lab Supplies/Labels

Optimization of Viable Glioblastoma Cryopreservation for Establishment of Primary Tumor Cell Cultures

 

Background: Technologies related to the establishment of primary tumor cell cultures from solid tumors, including glioblastoma, are increasingly important to oncology research and practice. However, processing of fresh tumor specimens for establishment of primary cultures on the day of surgical collection is logistically difficult. The feasibility of viable cryopreservation of glioblastoma specimens, allowing for primary culture establishment weeks to months after surgical tumor collection and freezing, was demonstrated by Mullins et al. in 2013, with a success rate of 59% that was not significantly lower than that achieved with fresh tumor tissue. However, research targeting optimization of viable glioblastoma cryopreservation protocols for establishment of primary tumor cultures has been limited.
Objectives: The objective of this study was to optimize glioblastoma cryopreservation methods for viable cryobanking and to determine if two-dimensional (2D) or three-dimensional (3D) culture conditions were more supportive of glioblastoma growth after thawing of frozen tumor specimens.
Methods: Portions of eight human glioblastoma specimens were cryopreserved by four different protocols differing in the time of enzymatic digestion (before or after cryopreservation), and in the type of cryopreservation media (CryoStor CS10 or 10% dimethyl sulfoxide and 90% fetal calf serum). After 1 month, frozen tissues were thawed, enzymatically digested, if not digested before, and used for initiation of 2D or 3D primary tumor cultures to determine viability.
Results: Among the tested cryopreservation and culturing protocols, the most efficient combinations of cryopreservation and culture were those associated with the use of CryoStor CS10 cryopreservation medium, enzymatic digestion before freezing, and 2D culturing after thawing with a successful culture rate of 8 out of 8 cases (100%). Two-dimensional cultures were in general more efficient for the support of tumor cell growth after thawing than 3D cultures.

Metformin partially reverses the inhibitory effect of co-culture with ER-/PR-/HER2+ breast cancer cells on biomarkers of monocyte antitumor activity

Background: Immune activities of monocytes (MOs) can be altered within the microenvironment of solid malignancies, including breast cancer. Metformin (1,1-dimethylbiguanide hydrochloride, MET), has been shown to decrease tumor cell proliferation, but its effects have yet to be explored with respect to MOs (monocytes) activity during their crosstalk with breast cancer cells. Here, we investigated the effects of MET on overall phenotypic functional activities, including cellular immunometabolism and protective redox signaling based-biomarkers, intracellular free calcium ions (ifCa2+), phagocytosis and co-operative cytokines (IFN-γ and IL-10) of autologous MOs before and during their interplay with primary ER-/PR-/HER2+ breast cancer cells.
Methods: Human primary breast cancer cells were either cultured alone or co-cultured with autologous MOs before treatment with MET.
Results: MET downregulated breast cancer cell proliferation and phagocytosis, while having no significant effect on the ratio of phosphorylated Akt (p-Akt) to total Akt. Additionally, we observed that, in the absence of MET treatment, the levels of lactate dehydrogenase (LDH)-based cytotoxicity, catalase, ifCa2+, IL-10 and arginase activity were significantly reduced in co-cultures compared to levels in MOs cultured alone whereas levels of inducible nitric oxide synthase (iNOS) activity were significantly increased. In contrast, MET treatment reduced the effects measured in co-culture on the levels of LDH-based cytotoxicity, arginase activity, catalase, ifCa2+, and IFN-γ. MET also induced upregulation of both iNOS and arginase in MO cells, although the increase did not reach significant difference for iNOS activity.
Moreover, MET induced a robust increase of superoxide dismutase (SOD) activity in MOs, but not in MOs co-cultured with breast cancer cells. Furthermore, MET markedly upregulated the levels of IFN-γ production and downregulated those of IL-10 in isolated MOs, while inducing a slight opposing up-regulation of IL-10 production in co-cultures.
anti-CD4 T helper cells
502-A-01mg 0,1 mg
EUR 267.50
  • Category: Antibody, Signal Transduction Antibodies, mAb
Description: anti-CD4 T helper cells
anti-CD4 T helper cells
502-A-1000ug 1000 ug
EUR 1282.50
  • Category: Antibody, Signal Transduction Antibodies, mAb
Description: anti-CD4 T helper cells
CD45RA/CD45RO/CD3/CD4
45RAF245ROPE3PP14A1-50T 50 test
EUR 609.00
MagIso CD8 Memory T Cell Isolation Kit, Human
WHK-B026 20 tests, 200 tests Ask for price
Human Normal Peripheral Blood CD4+/CD25+ Regulatory T Cells (T reg), Cryopreserved
PBCD4-25-C2M NULL
EUR NULL
Human Normal Peripheral Blood CD4+/CD25+ Regulatory T Cells (T reg), Fresh
PBCD4-25-F2M NULL
EUR NULL
Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-10
EUR 566.00
Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-20
EUR 805.00
Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-30
EUR 1327.00
Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-40
EUR 1953.00
Human T-cell surface glycoprotein CD4 (CD4)
1-CSB-YP004935HU1
  • EUR 430.00
  • EUR 234.00
  • EUR 1508.00
  • EUR 642.00
  • EUR 1009.00
  • EUR 291.00
  • 0
  • 1
  • 2
  • 3
  • 4
  • 5
  • MW: 43.3 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human T-cell surface glycoprotein CD4(CD4),partial expressed in Yeast
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx009476
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 0
  • 1
  • 2
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx019049-100ug 100 ug
EUR 342.00
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx021410-02mg 0.2 mg
EUR 739.00
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx015800-100ul 100 ul
EUR 411.00
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx200030-100ug 100 ug
EUR 328.00
  • Shipped within 2-3 weeks.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx200854
  • EUR 272.00
  • EUR 411.00
  • 0
  • 1
  • Shipped within 2-3 weeks.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx111482
  • EUR 732.00
  • EUR 398.00
  • 0
  • 1
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx125313
  • EUR 495.00
  • EUR 704.00
  • EUR 356.00
  • 0
  • 1
  • 2
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx134635
  • EUR 356.00
  • EUR 565.00
  • EUR 300.00
  • 0
  • 1
  • 2
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx134645
  • EUR 356.00
  • EUR 565.00
  • EUR 300.00
  • 0
  • 1
  • 2
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx134646
  • EUR 356.00
  • EUR 565.00
  • EUR 300.00
  • 0
  • 1
  • 2
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx137099
  • EUR 356.00
  • EUR 523.00
  • 0
  • 1
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx146722-100ug 100 ug
EUR 439.00
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx146728-100ug 100 ug
EUR 439.00
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx159398
  • EUR 272.00
  • EUR 230.00
  • 0
  • 1
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159399-100ul 100 ul
EUR 356.00
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159400-100ul 100 ul
EUR 356.00
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159401-100ul 100 ul
EUR 356.00
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159402-100ul 100 ul
EUR 356.00
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159403-100ul 100 ul
EUR 439.00
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159404-100ul 100 ul
EUR 356.00
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159405-100ul 100 ul
EUR 356.00
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx159406-100ul 100 ul
EUR 356.00
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx139124-01mg 0.1 mg
EUR 356.00
  • Shipped within 5-12 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx139125-01mg 0.1 mg
EUR 356.00
  • Shipped within 5-12 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx139126-01mg 0.1 mg
EUR 356.00
  • Shipped within 5-12 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx139127-01mg 0.1 mg
EUR 356.00
  • Shipped within 5-12 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx140007-01mg 0.1 mg
EUR 286.00
  • Shipped within 5-12 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx140008-01mg 0.1 mg
EUR 286.00
  • Shipped within 5-12 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx140009-01mg 0.1 mg
EUR 286.00
  • Shipped within 5-12 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx140102-01mg 0.1 mg
EUR 314.00
  • Shipped within 5-12 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx140341-01mg 0.1 mg
EUR 356.00
  • Shipped within 5-12 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx140432-01mg 0.1 mg
EUR 356.00
  • Shipped within 5-12 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx012383-100ul 100 ul
EUR 411.00
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx028455-400ul 400 ul
EUR 523.00
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx028455-80l 80 µl
EUR 286.00
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx028456-400ul 400 ul
EUR 551.00
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx028456-80l 80 µl
EUR 321.00
  • Shipped within 5-10 working days.
T-Cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx242941
  • EUR 467.00
  • EUR 328.00
  • 0
  • 1
  • Shipped within 1-2 weeks.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx412648-025mg 0.25 mg
EUR 565.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx412680-01mg 0.1 mg
EUR 509.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx412727-01mg 0.1 mg
EUR 453.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx412755-05mg 0.5 mg
EUR 829.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx412884-02mg 0.2 mg
EUR 565.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx412885-05mg 0.5 mg
EUR 829.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx412888-01mg 0.1 mg
EUR 439.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx412891-25ug 25 ug
EUR 272.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413138-01mg 0.1 mg
EUR 439.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413139-025mg 0.25 mg
EUR 565.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413254-01mg 0.1 mg
EUR 453.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413340-025mg 0.25 mg
EUR 565.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413341-05mg 0.5 mg
EUR 829.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413342-01mg 0.1 mg
EUR 439.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413343-25ug 25 ug
EUR 272.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413612-2ml 2 ml
EUR 606.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413766-01mg 0.1 mg
EUR 509.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx413842-01mg 0.1 mg
EUR 453.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414255-025mg 0.25 mg
EUR 592.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414488-025mg 0.25 mg
EUR 565.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414492-25ug 25 ug
EUR 272.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx320336
  • EUR 300.00
  • EUR 244.00
  • 0
  • 1
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414713-025mg 0.25 mg
EUR 565.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414714-1mg 1 mg
EUR 829.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414765-025mg 0.25 mg
EUR 565.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414766-05mg 0.5 mg
EUR 829.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
20-abx326208
  • EUR 314.00
  • EUR 244.00
  • 0
  • 1
  • Shipped within 5-10 working days.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414768-01mg 0.1 mg
EUR 439.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414771-25ug 25 ug
EUR 272.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414950-02mg 0.2 mg
EUR 565.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx414951-25ug 25 ug
EUR 272.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx415067-1mg 1 mg
EUR 829.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx415068-01mg 0.1 mg
EUR 439.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx415070-025mg 0.25 mg
EUR 565.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx415071-1mg 1 mg
EUR 829.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx415352-01mg 0.1 mg
EUR 453.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx415359-01mg 0.1 mg
EUR 453.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx415473-025ml 0.25 ml
EUR 606.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx415540-01mg 0.1 mg
EUR 453.00
  • Shipped within 1 week.
T-cell Surface Glycoprotein CD4 (CD4) Antibody
abx415565-01mg 0.1 mg
EUR 453.00
  • Shipped within 1 week.