Transcriptomes of Major Proximal Tubule Cell Culture Models
Background: Cultured cell lines are widely used for research in the physiology, pathophysiology, toxicology, and pharmacology of the renal proximal tubule. The lines that are most appropriate for a given use depend upon the genes expressed. New tools for transcriptomic profiling using RNA sequencing (RNA-Seq) make it possible to catalog expressed genes in each cell line.
Methods: Fourteen different proximal tubule cell lines, representing six species, were grown on permeable supports under conditions specific for the respective lines. RNA-Seq followed standard procedures.
Results: Transcripts expressed in cell lines variably matched transcripts selectively expressed in native proximal tubule. Opossum kidney (OK) cells displayed the highest percentage match (45% of proximal marker genes [TPM threshold =15]), with pig kidney cells (LLC-PK1) close behind (39%). Lower-percentage matches were seen for various human lines, including HK-2 (26%), and lines from rodent kidneys, such as NRK-52E (23%). Nominally, identical OK cells from different sources differed substantially in expression of proximal tubule markers. Mapping cell line transcriptomes to gene sets for various proximal tubule functions (sodium and water transport, protein transport, metabolic functions, endocrine functions) showed that different lines may be optimal for experimentally modeling each function.
An online resource (https://esbl.nhlbi.nih.gov/JBrowse/KCT/) has been created to interrogate cell line transcriptome data. Proteomic analysis of NRK-52E cells confirmed low expression of many proximal tubule marker proteins.
Conclusions: No cell line fully matched the transcriptome of native proximal tubule cells. However, some of the lines tested are suitable for the study of particular metabolic and transport processes seen in the proximal tubule.
Fibroblast-like cells change gene expression of bone remodelling markers in transwell cultures
Introduction: Periprosthetic fibroblast-like cells (PPFs) play an important role in aseptic loosening of arthroplasties. Various studies have examined PPF behavior in monolayer culture systems. However, the periprosthetic tissue is a three-dimensional (3D) mesh, which allows the cells to interact in a multidirectional way. The expression of bone remodeling markers of fibroblast-like cells in a multilayer environment changes significantly versus monolayer cultures without the addition of particles or cytokine stimulation. Gene expression of bone remodeling markers was therefore compared in fibroblast-like cells from different origins and dermal fibroblasts under transwell culture conditions versus monolayer cultures.
 NeuroProgenitor Medium |
|
NM42400 |
Neuromics |
125 ml |
EUR 304 |
 Heller's Medium |
|
CP014-010 |
GenDepot |
10X1L |
EUR 99 |
Heller's Medium |
|
CP014-500 |
GenDepot |
50L |
EUR 126 |
 Hoagland's Medium |
|
CP015-010 |
GenDepot |
10X1L |
EUR 99 |
Hoagland's Medium |
|
CP015-500 |
GenDepot |
50L |
EUR 126 |
 Advanced Medium |
|
C0003-04 |
Addexbio |
RT 500 mL Bottle |
EUR 103 |
 Medium 199 |
|
C0012-01 |
Addexbio |
RT 500 mL Bottle |
EUR 105 |
 50ml TC Tubes, Conical, 440 units/box |
|
04-5540150 |
Gentaur Genprice |
440 units/box |
EUR 71 |
 GMP IL4, 50µg |
|
04-GMP-HU-IL4-50UG |
Gentaur Genprice |
50µg |
EUR 483 |
|
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques. |
 SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE |
|
04-GSB |
Gentaur Genprice |
|
|
|
|
|
Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You! |
 rHu IL 2 , 3MIU |
|
04-RHIL2-02F01 |
Gentaur Genprice |
1 vial |
EUR 249 |
|
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated. |
 rHu IL 2 , 3MIU , Lot 200908F02 |
|
04-RHIL2-08F02 |
Gentaur Genprice |
1 vial |
EUR 249 |
|
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated. |
 PRE-GMP rHu GM-CSF, Molgramostim-Leukoma |
|
04-RHUGM-CSF-7A10 |
Gentaur Genprice |
300 µg |
EUR 385 |
|
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD. |
 Monkeypox Virus Real Time PCR Kit |
|
ZD-0076-01 |
Gentaur Genprice |
25 tests/kit |
Ask for price |
|
|
|
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load. |
Monkeypox Virus Real Time PCR Kit |
|
ZD-0076-02 |
Gentaur Genprice |
25 tests/kit |
Ask for price |
|
|
|
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load. |
 Insect Cell Medium: TNM-FH Insect Culture Medium |
|
ABP-MED-10001 |
Allele Biotech |
1 liter |
Ask for price |
|
|
 Insect Cell Medium: Serum-Free Insect Culture Medium |
|
ABP-MED-10002 |
Allele Biotech |
1 liter |
Ask for price |
|
|
) Insect Cell Medium: Grace?s Insect Medium (Unsupplemented) |
|
ABP-MED-10004 |
Allele Biotech |
1 liter |
Ask for price |
|
|
 H Medium Broth |
|
DJ1019 |
Bio Basic |
100g |
EUR 60.88 |
|
|
 Schwann Growth Medium |
|
SGM001 |
Neuromics |
500 ml |
EUR 244 |
 Hepatocyte Maintenance Medium |
|
HM42600 |
Neuromics |
100 ml |
EUR 409 |
 Neuron Growth Medium |
|
HNM001 |
Neuromics |
500 ml |
EUR 331 |
 MCDB 131 Medium |
|
CM034-050 |
GenDepot |
500ml |
EUR 85 |
MCDB 131 Medium |
|
CM034-300 |
GenDepot |
6x500ml |
EUR 165 |
MCDB 131 Medium |
|
CM034-310 |
GenDepot |
10x500ml |
EUR 219 |
MCDB 131 Medium |
|
CM034-320 |
GenDepot |
20x500ml |
EUR 340 |
MCDB 131 Medium |
|
CM034-350 |
GenDepot |
50x500ml |
EUR 585 |
 Williams' Medium
E |
|
CM063-050 |
GenDepot |
500ml |
EUR 91 |
Williams' Medium
E |
|
CM063-300 |
GenDepot |
6x500ml |
EUR 266 |
Williams' Medium
E |
|
CM063-310 |
GenDepot |
10x500ml |
EUR 313 |
Williams' Medium
E |
|
CM063-320 |
GenDepot |
20x500ml |
EUR 604 |
Williams' Medium
E |
|
CM063-350 |
GenDepot |
50x500ml |
EUR 975 |
 Cereal Crops Medium |
|
CP003-005 |
GenDepot |
5L |
EUR 113 |
Cereal Crops Medium |
|
CP003-010 |
GenDepot |
10X1L |
EUR 169 |
 Kao Michayluk Medium |
|
CP017-010 |
GenDepot |
10X1L |
EUR 99 |
Kao Michayluk Medium |
|
CP017-500 |
GenDepot |
50L |
EUR 126 |
 Orchid Maintenance Medium |
|
CP037-010 |
GenDepot |
10X1L |
EUR 109 |
Orchid Maintenance Medium |
|
CP037-500 |
GenDepot |
50L |
EUR 138 |
 Woody Plants Medium |
|
CP040-010 |
GenDepot |
10X1L |
EUR 101 |
Woody Plants Medium |
|
CP040-500 |
GenDepot |
50L |
EUR 126 |
 EverBrite Mounting Medium |
|
23001 |
Biotium |
10mL |
EUR 178 |
|
Description: Minimum order quantity: 1 unit of 10mL |
 AddexBio Cryopreservation Medium |
|
C0002-20mL |
Addexbio |
RT vial |
EUR 104 |
 Aqueous Mounting Medium |
|
AR1018 |
BosterBio |
10mL X5 (Enough for 800-1200 assays) |
EUR 80 |
 Antifade Mounting Medium |
|
AR1109 |
BosterBio |
10mL (for 1000 assays) |
EUR 65 |
 RPMI 1640 Medium |
|
abx098879-500ml |
Abbexa |
500 ml |
EUR 175 |
|
|
 Sepadextran-25 Medium |
|
SD25M-100 |
ABTBeads |
100 g |
EUR 271 |
Sepadextran-25 Medium |
|
SD25M-500 |
ABTBeads |
500 g |
EUR 1036 |
 Sepadextran-50 Medium |
|
SD50M-100 |
ABTBeads |
100 g |
EUR 344 |
Sepadextran-50 Medium |
|
SD50M-500 |
ABTBeads |
500 g |
EUR 1285 |
 SuperMOUNT? Mounting Medium |
|
1211-20 |
Biovision |
|
EUR 201 |
 Antibody) Angiotensin III (Ang III) Antibody |
|
20-abx101595 |
Abbexa |
-
EUR 425.00
-
EUR 133.00
-
EUR 1205.00
-
EUR 578.00
-
EUR 328.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
|
 Antibody) Bovine Antithrombin III (AT III) Antibody |
|
22111-05011 |
AssayPro |
150 ug |
EUR 217 |
 Protein (OVA)) Angiotensin III (Ang III) Protein (OVA) |
|
20-abx651227 |
Abbexa |
-
EUR 258.00
-
EUR 189.00
-
EUR 578.00
-
EUR 286.00
-
EUR 230.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
|
 III Antibody |
|
1-CSB-PA303116LA01ECY |
Cusabio |
|
|
|
|
|
Description: A polyclonal antibody against III. Recognizes III from Enterobacteria phage M13. This antibody is Unconjugated. Tested in the following application: ELISA |
 Sporidesmolide III |
|
S098-0.5MG |
TOKU-E |
0.5 mg |
EUR 318 |
Sporidesmolide III |
|
S098-2.5MG |
TOKU-E |
2.5 mg |
EUR 1040 |
 Filipin III |
|
F021-1MG |
TOKU-E |
1 mg |
EUR 261 |
Filipin III |
|
F021-5MG |
TOKU-E |
5 mg |
EUR 839 |
 Corynecin III |
|
C186-1MG |
TOKU-E |
1 mg |
EUR 154 |
Corynecin III |
|
C186-5MG |
TOKU-E |
5 mg |
EUR 465 |
Filipin III |
|
20-abx076644 |
Abbexa |
|
|
|
|
 Jingzhaotoxin III |
|
B5763-.1 |
ApexBio |
100 ug |
EUR 601 |
Filipin III |
|
B6034-.5 |
ApexBio |
500 µg |
EUR 173 |
Methods: PPFs from periprosthetic tissues (n = 12), osteoarthritic (OA) synovial fibroblast-like cells (SFs) (n = 6), and dermal fibroblasts (DFs) were cultured in monolayer (density 5.5 × 103/cm2) or multilayer cultures (density 8.5 × 105/cm2) for 10 or 21 days. Cultures were examined via histology, TRAP staining, immunohistochemistry (anti-S100a4), and quantitative real-time PCR.
Results: Fibroblast-like cells (PPFs/SFs) and dermal fibroblasts significantly increased the expression of RANKL and significantly decreased the expression of ALP, COL1A1, and OPG in multilayer cultures. PPFs and SFs in multilayer cultures further showed a higher expression of cathepsin K, MMP-13, and TNF-α. In multilayer PPF cultures, the mRNA level of TRAP was also found to be significantly increased.
Conclusion: The multilayer cultures are able to induce significant expression changes in fibroblast-like cells depending on the nature of cellular origin without the addition of any further stimulus. This system might be a useful tool to get more in vivo like results regarding fibroblast-like cell cultures.
A novel method of cell culture based on the microfluidic chip for regulation of cell density
The regulation of cell density is an important segment in microfluidic cell culture, particularly in the repeated assays. Traditionally, the consistent cell density is difficult to achieve owing to the inaccurate regulation of cell density with manual feedback. A novel cell culture method with automatic feedback is proposed for real-time regulation of cell density based on microfluidic chip in this paper. Here, an integrated microfluidic system combining cell culture, density detection and control of proliferation rate was developed.
Interdigital electrode structures (IDES) were sputtered on the microchannel for automatically to provide the real-time feedback information of impedance. The most sensitive frequency was studied to improve the detection resolution of the sensing chip. Cells were cultured on the chip surface and cell density was detected by monitoring the alternation of the impedance. The feedback controller was established by least squares support vector machines (LS-SVM). Then, the cell proliferation rate was automatically controlled using the feedback controller to achieve the desired cell density in the repeated assays.
The results show that the standard error of this method is 2.8% indicating that the method can keep consistency of cell density in the repeated assays. This study provides a basis for improving the accuracy and repeatability in the further assays of finding the optimal drug concentration. This article is protected by copyright. All rights reserved.
Effects of Oxygen and Glucose on Bone Marrow Mesenchymal Stem Cell Culture
This study determines whether the viability of mesenchymal stem cell (MSC) in vitro is most sensitive to oxygen supply, energetic substrate supply, or accumulation of lactate. Mouse unmodified (wild type (WT)) and erythropoietin (EPO) gene-modified MSC is cultured for 7 days in normoxic (21%) and anoxic conditions. WT-MSC is cultured in anoxia for 45 days in high and regular glucose media and both have similar viability when compared to their normoxic controls at 7 days. Protein production of EPO-MSC is unaffected by the absence of oxygen.
MSC doubling time and post-anoxic exposure is increased (WT: 32.3-73.3 h; EPO: 27.2-115 h). High glucose leads to a 37% increase in cell viability at 13 days and 17% at 30 days, indicating that MSC anoxic survival is affected by supply of metabolic substrate. However, after 30 days, little difference in viability is found, and at 45 days, complete cell death occurs in both the conditions. This death cannot be attributed to lack of glucose or lactate levels. MSC stemness is retained for both osteogenic and adipogenic differentiations. The absence of oxygen increases the doubling time of MSC but does not affect their viability, protein production, or differentiation capacity.
Monolayer/spheroid co-culture of cells on a PDMS well plate mediated by selective polydopamine coating
Here, we have selectively coated polydopamine (PDA) onto a polydimethylsiloxane (PDMS) well plate to enable the cell co-culture of a monolayer and spheroids in a semi-segregated manner. During the coating process, the contact between the PDA solution and PDMS well plate was limited to the outer flat surface because the strong hydrophobicity of PDMS prevented the access of the PDA solution into the concave structures. This resulted in a spatially-defined coating of PDA. The success of PDA coating was evidenced by measuring the water contact angle, observing the liquid-air interface, and via PDA-specific metallization.
This platform provides a simultaneous cell culture in both a monolayer and spheroids employing either monotypic or heterotypic cells. For the monotypic culture, mesenchymal stem cells (MSCs) were seeded over the well plate to concurrently generate the monolayer and spheroids. In the heterotypic culture, MSCs were first seeded into the wells to form spheroids. Then, human umbilical vein endothelial cells (HUVECs) were added over the flat surface of the well plate and allowed to form a monolayer. The microscopic observation and fluorescence-based cell staining confirmed the clear segregation between the monolayer and spheroids in both monotypic and heterotypic cultures. This new model could pave the way for the construction of a platform closely mimicking the physiological environment used to investigate cell-cell interactions and communications applicable for drug screening.
 PSMB8 Antibody |
|
BF0434 |
Affbiotech |
200ul |
EUR 376 |
|
Description: PSMB8 antibody detects endogenous levels of total PSMB8. |
 PSMB8 antibody |
|
70R-19589 |
Fitzgerald |
50 ul |
EUR 435 |
|
Description: Rabbit polyclonal PSMB8 antibody |
PSMB8 antibody |
|
70R-13550 |
Fitzgerald |
100 ul |
EUR 457 |
|
Description: Affinity purified Rabbit polyclonal PSMB8 antibody |
 Anti-PSMB8 antibody |
|
STJ29479 |
St John's Laboratory |
100 µl |
EUR 277 |
|
Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. |
Anti-PSMB8 antibody |
|
STJ11100809 |
St John's Laboratory |
100 µl |
EUR 413 |
|
Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. |
 PSMB8 Conjugated Antibody |
|
C43134 |
SAB |
100ul |
EUR 397 |
 PSMB8 siRNA |
|
20-abx930156 |
Abbexa |
|
|
|
|
PSMB8 siRNA |
|
20-abx930157 |
Abbexa |
|
|
|
|
PSMB8 siRNA |
|
20-abx904317 |
Abbexa |
|
|
|
|
 PSMB8 Antibody, HRP conjugated |
|
1-CSB-PA018886LB01HU |
Cusabio |
|
|
|
|
|
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
 PSMB8 Antibody, FITC conjugated |
|
1-CSB-PA018886LC01HU |
Cusabio |
|
|
|
|
|
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
 PSMB8 Antibody, Biotin conjugated |
|
1-CSB-PA018886LD01HU |
Cusabio |
|
|
|
|
|
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
 PSMB8 Rabbit pAb |
|
A7340-100ul |
Abclonal |
100 ul |
EUR 308 |
PSMB8 Rabbit pAb |
|
A7340-200ul |
Abclonal |
200 ul |
EUR 459 |
PSMB8 Rabbit pAb |
|
A7340-20ul |
Abclonal |
20 ul |
EUR 183 |
PSMB8 Rabbit pAb |
|
A7340-50ul |
Abclonal |
50 ul |
EUR 223 |
 PSMB8 Blocking Peptide |
|
BF0434-BP |
Affbiotech |
1mg |
EUR 195 |
) anti-PSMB8 (1A5) |
|
LF-MA30618 |
Abfrontier |
100 ul |
EUR 527 |
|
Description: Mouse Monoclonal to PSMB8 |
 Anti-PSMB8 / LMP7 Monoclonal Antibody |
|
M02188-2 |
BosterBio |
100ug |
EUR 397 |
|
Description: Rabbit Monoclonal PSMB8 / LMP7 Antibody. Validated in IP, IF, WB and tested in Human, Mouse, Rat. |
 Monoclonal PSMB8 Antibody, Clone: 1A5 |
|
AMM02864G |
Leading Biology |
0.1ml |
EUR 528 |
|
Description: A Monoclonal antibody against Human PSMB8. The antibodies are raised in Mouse and are from clone 1A5. This antibody is applicable in WB and IHC, ICC, E |
 Mouse PSMB8 shRNA Plasmid |
|
20-abx971337 |
Abbexa |
|
|
|
|
 Rat PSMB8 shRNA Plasmid |
|
20-abx984734 |
Abbexa |
|
|
|
|
 Human PSMB8 shRNA Plasmid |
|
20-abx953843 |
Abbexa |
|
|
|
|
) PSMB8 protein (His tag) |
|
80R-2608 |
Fitzgerald |
100 ug |
EUR 322 |
|
Description: Purified recombinant PSMB8 protein |
) PSMB8 Recombinant Protein (Mouse) |
|
RP165287 |
ABM |
100 ug |
Ask for price |
) PSMB8 Recombinant Protein (Human) |
|
RP085356 |
ABM |
100 ug |
Ask for price |
) PSMB8 Recombinant Protein (Rat) |
|
RP222629 |
ABM |
100 ug |
Ask for price |
 (M01), Clone: 1B3) Monoclonal PSMB8 Antibody (monoclonal) (M01), Clone: 1B3 |
|
AMM03954G |
Leading Biology |
0.1mg |
EUR 484 |
|
Description: A Monoclonal antibody against Human PSMB8 (monoclonal) (M01). The antibodies are raised in Mouse and are from clone 1B3. This antibody is applicable in WB, IHC and IF, IP, E |
 Antibody) Proteasome Subunit Beta Type-8 (PSMB8) Antibody |
|
20-abx114776 |
Abbexa |
|
|
|
|
Proteasome Subunit Beta Type-8 (PSMB8) Antibody |
|
20-abx141396 |
Abbexa |
-
EUR 370.00
-
EUR 606.00
-
EUR 300.00
|
|
|
|
Proteasome Subunit Beta Type-8 (PSMB8) Antibody |
|
20-abx006453 |
Abbexa |
-
EUR 411.00
-
EUR 592.00
-
EUR 182.00
-
EUR 314.00
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
|
Proteasome Subunit Beta Type-8 (PSMB8) Antibody |
|
abx011689-100ul |
Abbexa |
100 ul |
EUR 411 |
|
|
Proteasome Subunit Beta Type-8 (PSMB8) Antibody |
|
abx028154-400ul |
Abbexa |
400 ul |
EUR 523 |
|
|
Proteasome Subunit Beta Type-8 (PSMB8) Antibody |
|
abx028154-80l |
Abbexa |
80 µl |
EUR 286 |
|
|
 Antibody) Proteasome subunit beta type-8 (PSMB8) Antibody |
|
20-abx211178 |
Abbexa |
|
|
|
|
Proteasome subunit beta type-8 (PSMB8) Antibody |
|
20-abx211851 |
Abbexa |
|
|
|
|
 [KO Validated] PSMB8 Rabbit pAb |
|
A19933-100ul |
Abclonal |
100 ul |
EUR 410 |
[KO Validated] PSMB8 Rabbit pAb |
|
A19933-200ul |
Abclonal |
200 ul |
EUR 571 |
[KO Validated] PSMB8 Rabbit pAb |
|
A19933-20ul |
Abclonal |
20 ul |
EUR 221 |
[KO Validated] PSMB8 Rabbit pAb |
|
A19933-50ul |
Abclonal |
50 ul |
EUR 287 |
 (OKEH03975)) PSMB8 ELISA Kit (Dog) (OKEH03975) |
|
OKEH03975 |
Aviva Systems Biology |
96 Wells |
EUR 844 |
|
Description: Description of target: ;Species reactivity: Dog;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.064 ng/mL |
 (OKCD02695)) PSMB8 ELISA Kit (Human) (OKCD02695) |
|
OKCD02695 |
Aviva Systems Biology |
96 Wells |
EUR 792 |
|
Description: Description of target: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.117 ng/mL |
 (OKEH07770)) PSMB8 ELISA Kit (Human) (OKEH07770) |
|
OKEH07770 |
Aviva Systems Biology |
96 Wells |
EUR 1092 |
|
Description: Description of target: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.06ng/mL |
 (pORF)) Psmb8 ORF Vector (Rat) (pORF) |
|
ORF074211 |
ABM |
1.0 ug DNA |
EUR 506 |
 (pORF)) PSMB8 ORF Vector (Human) (pORF) |
|
ORF028453 |
ABM |
1.0 ug DNA |
EUR 405 |
 (pORF)) Psmb8 ORF Vector (Mouse) (pORF) |
|
ORF055097 |
ABM |
1.0 ug DNA |
EUR 506 |
) PSMB8 sgRNA CRISPR Lentivector set (Human) |
|
K1740301 |
ABM |
3 x 1.0 ug |
EUR 339 |
) Psmb8 sgRNA CRISPR Lentivector set (Rat) |
|
K6921501 |
ABM |
3 x 1.0 ug |
EUR 339 |
) Psmb8 sgRNA CRISPR Lentivector set (Mouse) |
|
K4425601 |
ABM |
3 x 1.0 ug |
EUR 339 |
) Human Proteasome subunit beta type-8 (PSMB8) |
|
1-CSB-EP018886HU |
Cusabio |
-
EUR 380.00
-
EUR 214.00
-
EUR 1309.00
-
EUR 560.00
-
EUR 873.00
-
EUR 262.00
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
|
|
Description: Recombinant Human Proteasome subunit beta type-8(PSMB8) expressed in E.coli |
 (Target 1)) PSMB8 sgRNA CRISPR Lentivector (Human) (Target 1) |
|
K1740302 |
ABM |
1.0 ug DNA |
EUR 154 |
 (Target 2)) PSMB8 sgRNA CRISPR Lentivector (Human) (Target 2) |
|
K1740303 |
ABM |
1.0 ug DNA |
EUR 154 |
 (Target 3)) PSMB8 sgRNA CRISPR Lentivector (Human) (Target 3) |
|
K1740304 |
ABM |
1.0 ug DNA |
EUR 154 |
 (Target 1)) Psmb8 sgRNA CRISPR Lentivector (Rat) (Target 1) |
|
K6921502 |
ABM |
1.0 ug DNA |
EUR 154 |
 (Target 2)) Psmb8 sgRNA CRISPR Lentivector (Rat) (Target 2) |
|
K6921503 |
ABM |
1.0 ug DNA |
EUR 154 |
 (Target 3)) Psmb8 sgRNA CRISPR Lentivector (Rat) (Target 3) |
|
K6921504 |
ABM |
1.0 ug DNA |
EUR 154 |
 (Target 1)) Psmb8 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
|
K4425602 |
ABM |
1.0 ug DNA |
EUR 154 |
 PSMB8 3'UTR GFP Stable Cell Line |
|
TU069046 |
ABM |
1.0 ml |
EUR 1394 |
 (Target 2)) Psmb8 sgRNA CRISPR Lentivector (Mouse) (Target 2) |
|
K4425603 |
ABM |
1.0 ug DNA |
EUR 154 |
 (Target 3)) Psmb8 sgRNA CRISPR Lentivector (Mouse) (Target 3) |
|
K4425604 |
ABM |
1.0 ug DNA |
EUR 154 |
 (pPB-C-His)) PSMB8 Protein Vector (Rat) (pPB-C-His) |
|
PV296842 |
ABM |
500 ng |
EUR 603 |
 (pPB-N-His)) PSMB8 Protein Vector (Rat) (pPB-N-His) |
|
PV296843 |
ABM |
500 ng |
EUR 603 |
 (pPM-C-HA)) PSMB8 Protein Vector (Rat) (pPM-C-HA) |
|
PV296844 |
ABM |
500 ng |
EUR 603 |
 (pPM-C-His)) PSMB8 Protein Vector (Rat) (pPM-C-His) |
|
PV296845 |
ABM |
500 ng |
EUR 603 |
 (pPB-C-His)) PSMB8 Protein Vector (Human) (pPB-C-His) |
|
PV113810 |
ABM |
500 ng |
EUR 552 |
 (pPB-N-His)) PSMB8 Protein Vector (Human) (pPB-N-His) |
|
PV113811 |
ABM |
500 ng |
EUR 552 |
 (pPM-C-HA)) PSMB8 Protein Vector (Human) (pPM-C-HA) |
|
PV113812 |
ABM |
500 ng |
EUR 552 |
 (pPM-C-His)) PSMB8 Protein Vector (Human) (pPM-C-His) |
|
PV113813 |
ABM |
500 ng |
EUR 552 |
 Recombinant Human PSMB8 Protein, His, E.coli-1mg |
|
QP13190-1mg |
EnQuireBio |
1mg |
EUR 2757 |
 Recombinant Human PSMB8 Protein, His, E.coli-20ug |
|
QP13190-20ug |
EnQuireBio |
20ug |
EUR 201 |
 Recombinant Human PSMB8 Protein, His, E.coli-5ug |
|
QP13190-5ug |
EnQuireBio |
5ug |
EUR 155 |
 (pPB-C-His)) PSMB8 Protein Vector (Mouse) (pPB-C-His) |
|
PV220386 |
ABM |
500 ng |
EUR 603 |
 (pPB-N-His)) PSMB8 Protein Vector (Mouse) (pPB-N-His) |
|
PV220387 |
ABM |
500 ng |
EUR 603 |
 (pPM-C-HA)) PSMB8 Protein Vector (Mouse) (pPM-C-HA) |
|
PV220388 |
ABM |
500 ng |
EUR 603 |
 (pPM-C-His)) PSMB8 Protein Vector (Mouse) (pPM-C-His) |
|
PV220389 |
ABM |
500 ng |
EUR 603 |
 Psmb8 3'UTR Luciferase Stable Cell Line |
|
TU216964 |
ABM |
1.0 ml |
Ask for price |
 PSMB8 3'UTR Luciferase Stable Cell Line |
|
TU019046 |
ABM |
1.0 ml |
EUR 1394 |
 Psmb8 3'UTR GFP Stable Cell Line |
|
TU266964 |
ABM |
1.0 ml |
Ask for price |
Psmb8 3'UTR Luciferase Stable Cell Line |
|
TU117173 |
ABM |
1.0 ml |
Ask for price |
Psmb8 3'UTR GFP Stable Cell Line |
|
TU167173 |
ABM |
1.0 ml |
Ask for price |
 (CMV) (pLenti-GIII-CMV)) PSMB8 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV) |
|
LV700471 |
ABM |
1.0 ug DNA |
EUR 514 |
 (UbC) (pLenti-GIII-UbC)) PSMB8 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC) |
|
LV700475 |
ABM |
1.0 ug DNA |
EUR 514 |
 (EF1a) (pLenti-GIII-EF1a)) PSMB8 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a) |
|
LV700476 |
ABM |
1.0 ug DNA |
EUR 514 |
 PSMB8 Proteasome Subunit Beta Type 8 Human Recombinant Protein |
|
PROTP28062 |
BosterBio |
Regular: 20ug |
EUR 317 |
|
Description: PSMB8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 229 amino acids (73-276 a.a.) and having a molecular mass of 25.4kDa. PSMB8 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
 Dog PSMB8/ Proteasome subunit beta type-8 ELISA Kit |
|
E0091Do |
Sunlong |
1 Kit |
EUR 717 |
 Human PSMB8/ Proteasome subunit beta type-8 ELISA Kit |
|
E2074Hu |
Sunlong |
1 Kit |
EUR 605 |
 Rat Psmb8/ Proteasome subunit beta type-8 ELISA Kit |
|
E0811Ra |
Sunlong |
1 Kit |
EUR 646 |
 Mouse Psmb8/ Proteasome subunit beta type-8 ELISA Kit |
|
E1218Mo |
Sunlong |
1 Kit |
EUR 632 |
 ELISA Kit) Monkey Low Molecular Mass Protein 7 (PSMB8) ELISA Kit |
|
abx360252-96tests |
Abbexa |
96 tests |
EUR 825 |
|
|
 ELISA Kit) Pig Low Molecular Mass Protein 7 (PSMB8) ELISA Kit |
|
abx361997-96tests |
Abbexa |
96 tests |
EUR 825 |
|
|
 ELISA Kit) Rabbit Low Molecular Mass Protein 7 (PSMB8) ELISA Kit |
|
abx362317-96tests |
Abbexa |
96 tests |
EUR 825 |
|
|
 ELISA Kit) Human Low Molecular Mass Protein 7 (PSMB8) ELISA Kit |
|
abx351643-96tests |
Abbexa |
96 tests |
EUR 707 |
|
|
 ELISA Kit) Chicken Low Molecular Mass Protein 7 (PSMB8) ELISA Kit |
|
abx356091-96tests |
Abbexa |
96 tests |
EUR 825 |
|
|
 ELISA Kit) Cow Low Molecular Mass Protein 7 (PSMB8) ELISA Kit |
|
abx515621-96tests |
Abbexa |
96 tests |
EUR 911 |
|
|
Tags:
culture cells definition,
culture cells in pure serum,
culture cells meaning,
pcr,
pcr covid,
pcr covid 19 test,
pcr cycle,
pcr definition,
pcr inhibitor,
pcr process,
pcr rapid test,
pcr steps,
pcr test near me,
pcr testing accuracy,
pcr tests,
pcr vs antigen testing,
pcram,
pcrb,
pcre,
pcre real estate,
pcrfy,
pcrfy stock price,
pcrichard&sons,
pcrichard.com,
pcrisk,
pcrn,
pcrsauto
