Transcriptomes of Major Proximal Tubule Cell Culture Models

Background: Cultured cell lines are widely used for research in the physiology, pathophysiology, toxicology, and pharmacology of the renal proximal tubule. The lines that are most appropriate for a given use depend upon the genes expressed. New tools for transcriptomic profiling using RNA sequencing (RNA-Seq) make it possible to catalog expressed genes in each cell line.

Methods: Fourteen different proximal tubule cell lines, representing six species, were grown on permeable supports under conditions specific for the respective lines. RNA-Seq followed standard procedures.

Results: Transcripts expressed in cell lines variably matched transcripts selectively expressed in native proximal tubule. Opossum kidney (OK) cells displayed the highest percentage match (45% of proximal marker genes [TPM threshold =15]), with pig kidney cells (LLC-PK1) close behind (39%). Lower-percentage matches were seen for various human lines, including HK-2 (26%), and lines from rodent kidneys, such as NRK-52E (23%). Nominally, identical OK cells from different sources differed substantially in expression of proximal tubule markers. Mapping cell line transcriptomes to gene sets for various proximal tubule functions (sodium and water transport, protein transport, metabolic functions, endocrine functions) showed that different lines may be optimal for experimentally modeling each function.

An online resource (https://esbl.nhlbi.nih.gov/JBrowse/KCT/) has been created to interrogate cell line transcriptome data. Proteomic analysis of NRK-52E cells confirmed low expression of many proximal tubule marker proteins.

Conclusions: No cell line fully matched the transcriptome of native proximal tubule cells. However, some of the lines tested are suitable for the study of particular metabolic and transport processes seen in the proximal tubule.

Introduction: Periprosthetic fibroblast-like cells (PPFs) play an important role in aseptic loosening of arthroplasties. Various studies have examined PPF behavior in monolayer culture systems. However, the periprosthetic tissue is a three-dimensional (3D) mesh, which allows the cells to interact in a multidirectional way. The expression of bone remodeling markers of fibroblast-like cells in a multilayer environment changes significantly versus monolayer cultures without the addition of particles or cytokine stimulation. Gene expression of bone remodeling markers was therefore compared in fibroblast-like cells from different origins and dermal fibroblasts under transwell culture conditions versus monolayer cultures.

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti chicken secondary antibody
K3171250-III	Biochain	2500 cm2	EUR 310
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Mounting Medium
4300	Virostat	3 ml	EUR 180.5
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein.

VALI Medium
450	AthenaES	500 ml	EUR 265

BEGM Medium
451	AthenaES	500 ml	EUR 152

DC MEDIUM
D04-117-10kg	Alphabiosciences	10 kg	EUR 1579

DC MEDIUM
D04-117-2kg	Alphabiosciences	2kg	EUR 382

DC MEDIUM
D04-117-500g	Alphabiosciences	500 g	EUR 140

EC MEDIUM
E05-100-10kg	Alphabiosciences	10 kg	EUR 814

EC MEDIUM
E05-100-2Kg	Alphabiosciences	2 Kg	EUR 216

EC MEDIUM
E05-100-500g	Alphabiosciences	500 g	EUR 95

Advanced Medium
C0003-04	Addexbio	RT 500 mL Bottle	EUR 103

Medium 199
C0012-01	Addexbio	RT 500 mL Bottle	EUR 105

COLIFORM MEDIUM
C03-127-10kg	Alphabiosciences	10 kg	EUR 1324

COLIFORM MEDIUM
C03-127-2kg	Alphabiosciences	2kg	EUR 327

COLIFORM MEDIUM
C03-127-500g	Alphabiosciences	500 g	EUR 125

Heller's Medium
CP014-010	GenDepot	10X1L	EUR 99

Heller's Medium
CP014-500	GenDepot	50L	EUR 126

Hoagland's Medium
CP015-010	GenDepot	10X1L	EUR 99

Hoagland's Medium
CP015-500	GenDepot	50L	EUR 126

HLP MEDIUM
H08-107-10kg	Alphabiosciences	10 kg	EUR 2278

HLP MEDIUM
H08-107-2Kg	Alphabiosciences	2 Kg	EUR 534

HLP MEDIUM
H08-107-500g	Alphabiosciences	500 g	EUR 182

NeuroProgenitor Medium
NM42400	Neuromics	125 ml	EUR 304

SIM MEDIUM
S19-110-10kg	Alphabiosciences	10 kg	EUR 1021

SIM MEDIUM
S19-110-2kg	Alphabiosciences	2kg	EUR 261

SIM MEDIUM
S19-110-500g	Alphabiosciences	500 g	EUR 107

SOB MEDIUM
S19-124-10kg	Alphabiosciences	10 kg	EUR 965

SOB MEDIUM
S19-124-2kg	Alphabiosciences	2kg	EUR 249

SOB MEDIUM
S19-124-500g	Alphabiosciences	500 g	EUR 104

A Medium
DJ1018	Bio Basic	100g	EUR 84.8
Product category: Culture Media/Medium

M9 Medium
SD7024	Bio Basic	250g	EUR 71.75
Product category: Culture Media/Medium

M9CA Medium
SD7025	Bio Basic	250g	EUR 71.75
Product category: Culture Media/Medium

YM Medium
SD7031	Bio Basic	250g	EUR 70.45
Product category: Culture Media/Medium

TYGPN Medium
SD7032	Bio Basic	250g	EUR 70.01
Product category: Culture Media/Medium

M63 Medium
SD7033	Bio Basic	250g	EUR 71.75
Product category: Culture Media/Medium

50ml TC Tubes, Conical, 440 units/box
04-5540150	Gentaur Genprice	440 units/box	EUR 71

GMP IL4, 50µg
04-GMP-HU-IL4-50UG	Gentaur Genprice	50µg	EUR 483
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.

rHu IL 2 , 3MIU
04-RHIL2-02F01	Gentaur Genprice	1 vial	EUR 249
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

rHu IL 2 , 3MIU , Lot 200908F02
04-RHIL2-08F02	Gentaur Genprice	1 vial	EUR 249
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

PRE-GMP rHu GM-CSF, Molgramostim-Leukoma
04-RHUGM-CSF-7A10	Gentaur Genprice	300 µg	EUR 385
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD.

SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE
04-GSB	Gentaur Genprice	EUR 120.00 EUR 80.00	1L 500mL
Working procedure: 1) Invert the bottle a few times to ensure the solution is properly suspended. 2) Take the gel out of the gel tank and submerse the gel in enough SDS-Blue to cover the gel. 3) Bands will be visi Show more
Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You!

Insect Cell Medium: TNM-FH Insect Culture Medium
ABP-MED-10001	Allele Biotech	1 liter	Ask for price
Product line: Cell Culture Reagents Brand:

Insect Cell Medium: Serum-Free Insect Culture Medium
ABP-MED-10002	Allele Biotech	1 liter	Ask for price
Product line: Cell Culture Reagents Brand:

Insect Cell Medium: Grace?s Insect Medium (Unsupplemented)
ABP-MED-10004	Allele Biotech	1 liter	Ask for price
Product line: Cell Culture Reagents Brand:

EverBrite Mounting Medium
23001	Biotium	10mL	EUR 178
Description: Minimum order quantity: 1 unit of 10mL

Recovery Medium - 6x12mL
1711	Intact Genomics	4/PK	EUR 128

SuperMOUNT? Mounting Medium
1211-20	Biovision		EUR 201

Aqueous Mounting Medium
AR1018	BosterBio	10mL X5 (Enough for 800-1200 assays)	EUR 80

Antifade Mounting Medium
AR1109	BosterBio	10mL (for 1000 assays)	EUR 65

EC MEDIUM, MODIFIED
E05-108-10kg	Alphabiosciences	10 kg	EUR 858

EC MEDIUM, MODIFIED
E05-108-2Kg	Alphabiosciences	2 Kg	EUR 226

EC MEDIUM, MODIFIED
E05-108-500g	Alphabiosciences	500 g	EUR 98

A-1 MEDIUM
A01-116-10kg	Alphabiosciences	10 kg	EUR 1034

A-1 MEDIUM
A01-116-2Kg	Alphabiosciences	2 Kg	EUR 264

A-1 MEDIUM
A01-116-500g	Alphabiosciences	500 g	EUR 108

RPMI 1640 Medium
abx098879-500ml	Abbexa	500 ml	EUR 175
Shipped within 5-10 working days.

MCDB 131 Medium
CM034-050	GenDepot	500ml	EUR 85

MCDB 131 Medium
CM034-300	GenDepot	6x500ml	EUR 165

MCDB 131 Medium
CM034-310	GenDepot	10x500ml	EUR 219

MCDB 131 Medium
CM034-320	GenDepot	20x500ml	EUR 340

MCDB 131 Medium
CM034-350	GenDepot	50x500ml	EUR 585

Williams' Medium E
CM063-050	GenDepot	500ml	EUR 91

Williams' Medium E
CM063-300	GenDepot	6x500ml	EUR 266

Williams' Medium E
CM063-310	GenDepot	10x500ml	EUR 313

Williams' Medium E
CM063-320	GenDepot	20x500ml	EUR 604

Williams' Medium E
CM063-350	GenDepot	50x500ml	EUR 975

AddexBio Cryopreservation Medium
C0002-20mL	Addexbio	RT vial	EUR 104

CARY & BLAIR MEDIUM
C03-131-10kg	Alphabiosciences	10 kg	EUR 2185

CARY & BLAIR MEDIUM
C03-131-2Kg	Alphabiosciences	2 Kg	EUR 514

CARY & BLAIR MEDIUM
C03-131-500g	Alphabiosciences	500 g	EUR 176

Cereal Crops Medium
CP003-005	GenDepot	5L	EUR 113

Cereal Crops Medium
CP003-010	GenDepot	10X1L	EUR 169

Kao Michayluk Medium
CP017-010	GenDepot	10X1L	EUR 99

Kao Michayluk Medium
CP017-500	GenDepot	50L	EUR 126

Orchid Maintenance Medium
CP037-010	GenDepot	10X1L	EUR 109

Orchid Maintenance Medium
CP037-500	GenDepot	50L	EUR 138

Woody Plants Medium
CP040-010	GenDepot	10X1L	EUR 101

Woody Plants Medium
CP040-500	GenDepot	50L	EUR 126

Hepatocyte Maintenance Medium
HM42600	Neuromics	100 ml	EUR 409

Neuron Growth Medium
HNM001	Neuromics	500 ml	EUR 331

FLUID THIOGLYCOLLATE MEDIUM
F06-101-10kg	Alphabiosciences	10 kg	EUR 792

FLUID THIOGLYCOLLATE MEDIUM
F06-101-2kg	Alphabiosciences	2kg	EUR 211

FLUID THIOGLYCOLLATE MEDIUM
F06-101-500g	Alphabiosciences	500 g	EUR 94

Agarose, medium EEO
GE4750-100G	Glentham Life Sciences	100 g	EUR 166

Agarose, medium EEO
GE4750-500G	Glentham Life Sciences	500 g	EUR 429

FluoreGuard Mounting Medium
FMM030	ScyTek Laboratories	30 ml	EUR 96

FluoreGuard Mounting Medium
FMM060	ScyTek Laboratories	60 ml	EUR 116

FluoreGuard Mounting Medium
FMM500	ScyTek Laboratories	500 ml	EUR 327

FluoreGuard Mounting Medium
FMM999	ScyTek Laboratories	1000 ml	EUR 530

OF BASAL MEDIUM
O15-100-10kg	Alphabiosciences	10 kg	Ask for price

OF BASAL MEDIUM
O15-100-2kg	Alphabiosciences	2kg	Ask for price

OF BASAL MEDIUM
O15-100-500g	Alphabiosciences	500 g	Ask for price

MOTILITY TEST MEDIUM
M13-121-10kg	Alphabiosciences	10 kg	EUR 1528

MOTILITY TEST MEDIUM
M13-121-2Kg	Alphabiosciences	2 Kg	EUR 371

MOTILITY TEST MEDIUM
M13-121-500g	Alphabiosciences	500 g	EUR 137

Methods: PPFs from periprosthetic tissues (n = 12), osteoarthritic (OA) synovial fibroblast-like cells (SFs) (n = 6), and dermal fibroblasts (DFs) were cultured in monolayer (density 5.5 × 10³/cm²) or multilayer cultures (density 8.5 × 10⁵/cm²) for 10 or 21 days. Cultures were examined via histology, TRAP staining, immunohistochemistry (anti-S100a4), and quantitative real-time PCR.

Results: Fibroblast-like cells (PPFs/SFs) and dermal fibroblasts significantly increased the expression of RANKL and significantly decreased the expression of ALP, COL1A1, and OPG in multilayer cultures. PPFs and SFs in multilayer cultures further showed a higher expression of cathepsin K, MMP-13, and TNF-α. In multilayer PPF cultures, the mRNA level of TRAP was also found to be significantly increased.

Conclusion: The multilayer cultures are able to induce significant expression changes in fibroblast-like cells depending on the nature of cellular origin without the addition of any further stimulus. This system might be a useful tool to get more in vivo like results regarding fibroblast-like cell cultures.

This study determines whether the viability of mesenchymal stem cell (MSC) in vitro is most sensitive to oxygen supply, energetic substrate supply, or accumulation of lactate. Mouse unmodified (wild type (WT)) and erythropoietin (EPO) gene-modified MSC is cultured for 7 days in normoxic (21%) and anoxic conditions. WT-MSC is cultured in anoxia for 45 days in high and regular glucose media and both have similar viability when compared to their normoxic controls at 7 days. Protein production of EPO-MSC is unaffected by the absence of oxygen.

MSC doubling time and post-anoxic exposure is increased (WT: 32.3-73.3 h; EPO: 27.2-115 h). High glucose leads to a 37% increase in cell viability at 13 days and 17% at 30 days, indicating that MSC anoxic survival is affected by supply of metabolic substrate. However, after 30 days, little difference in viability is found, and at 45 days, complete cell death occurs in both the conditions. This death cannot be attributed to lack of glucose or lactate levels. MSC stemness is retained for both osteogenic and adipogenic differentiations. The absence of oxygen increases the doubling time of MSC but does not affect their viability, protein production, or differentiation capacity.

PSMB8 Antibody
43134-100ul	SAB	100ul	EUR 252

PSMB8 Antibody
1-CSB-PA116429	Cusabio	EUR 317.00 EUR 244.00	100ul 50ul
Form: Liquid Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100

PSMB8 Antibody
1-CSB-PA775839	Cusabio	EUR 317.00 EUR 244.00	100ul 50ul
Form: Liquid Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100

PSMB8 Antibody
BF0434	Affbiotech	200ul	EUR 376
Description: PSMB8 antibody detects endogenous levels of total PSMB8.

PSMB8 Antibody
1-CSB-PA018886GA01HU	Cusabio	EUR 597.00 EUR 333.00	150ul 50ul
Form: Liquid Buffer: PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20℃, Avoid freeze / thaw cycles. Antigen Affinity purified
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF

PSMB8 Antibody
1-CSB-PA018886LA01HU	Cusabio	EUR 317.00 EUR 335.00	100ul 50ul
Form: Liquid Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

PSMB8 Conjugated Antibody
C43134	SAB	100ul	EUR 397

Anti-PSMB8 antibody
STJ11100809	St John's Laboratory	100 µl	EUR 413
Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.

Anti-PSMB8 antibody
STJ29479	St John's Laboratory	100 µl	EUR 277
Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.

Psmb8/ Rat Psmb8 ELISA Kit
ELI-15652r	Lifescience Market	96 Tests	EUR 886

PSMB8 siRNA
20-abx904317	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol
Shipped within 5-10 working days.

PSMB8 siRNA
20-abx930156	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol
Shipped within 5-10 working days.

PSMB8 siRNA
20-abx930157	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol
Shipped within 5-10 working days.

pOTB7-PSMB8
PVT18497	Lifescience Market	2 ug	EUR 231

PSMB8 Antibody, HRP conjugated
1-CSB-PA018886LB01HU	Cusabio	EUR 317.00 EUR 335.00	100ul 50ul
Form: Liquid Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

PSMB8 Antibody, FITC conjugated
1-CSB-PA018886LC01HU	Cusabio	EUR 317.00 EUR 335.00	100ul 50ul
Form: Liquid Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

PSMB8 Antibody, Biotin conjugated
1-CSB-PA018886LD01HU	Cusabio	EUR 317.00 EUR 335.00	100ul 50ul
Form: Liquid Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

PSMB8 Blocking Peptide
BF0434-BP	Affbiotech	1mg	EUR 195

PSMB8 Rabbit pAb
A7340-100ul	Abclonal	100 ul	EUR 308

PSMB8 Rabbit pAb
A7340-200ul	Abclonal	200 ul	EUR 459

PSMB8 Rabbit pAb
A7340-20ul	Abclonal	20 ul	EUR 183

PSMB8 Rabbit pAb
A7340-50ul	Abclonal	50 ul	EUR 223

anti-PSMB8 (1A5)
LF-MA30618	Abfrontier	100 ul	EUR 527
Description: Mouse Monoclonal to PSMB8

Monoclonal PSMB8 Antibody, Clone: 1A5
AMM02864G	Leading Biology	0.1ml	EUR 528
Description: A Monoclonal antibody against Human PSMB8. The antibodies are raised in Mouse and are from clone 1A5. This antibody is applicable in WB and IHC, ICC, E

Anti-PSMB8 / LMP7 Monoclonal Antibody
M02188-2	BosterBio	100ug	EUR 397
Description: Rabbit Monoclonal PSMB8 / LMP7 Antibody. Validated in IP, IF, WB and tested in Human, Mouse, Rat.

PSMB8 protein (His tag)
80R-2608	Fitzgerald	100 ug	EUR 322
Description: Purified recombinant PSMB8 protein

Canine PSMB8 ELISA KIT
ELI-15651d	Lifescience Market	96 Tests	EUR 928

Rat PSMB8 shRNA Plasmid
20-abx984734	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg
Shipped within 15-20 working days.

Human PSMB8 shRNA Plasmid
20-abx953843	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg
Shipped within 15-20 working days.

Mouse PSMB8 shRNA Plasmid
20-abx971337	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg
Shipped within 15-20 working days.

PSMB8 Recombinant Protein (Human)
RP085356	ABM	100 ug	Ask for price

PSMB8 Recombinant Protein (Mouse)
RP165287	ABM	100 ug	Ask for price

PSMB8 Recombinant Protein (Rat)
RP222629	ABM	100 ug	Ask for price

Proteasome Subunit Beta Type-8 (PSMB8) Antibody
20-abx006453	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul
Shipped within 5-10 working days.

Proteasome Subunit Beta Type-8 (PSMB8) Antibody
abx028154-400ul	Abbexa	400 ul	EUR 523
Shipped within 5-10 working days.

Proteasome Subunit Beta Type-8 (PSMB8) Antibody
abx028154-80l	Abbexa	80 µl	EUR 286
Shipped within 5-10 working days.

Proteasome subunit beta type-8 (PSMB8) Antibody
20-abx211178	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul
Shipped within 5-10 working days.

Proteasome subunit beta type-8 (PSMB8) Antibody
20-abx211851	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul
Shipped within 5-10 working days.

Proteasome Subunit Beta Type-8 (PSMB8) Antibody
20-abx114776	Abbexa	EUR 732.00 EUR 398.00	150 ul 50 ul
Shipped within 5-10 working days.

Proteasome Subunit Beta Type-8 (PSMB8) Antibody
20-abx141396	Abbexa	EUR 370.00 EUR 606.00 EUR 300.00	100 ul 200 ul 50 ul
Shipped within 5-10 working days.

Proteasome Subunit Beta Type-8 (PSMB8) Antibody
abx011689-100ul	Abbexa	100 ul	EUR 411
Shipped within 5-10 working days.

Monoclonal PSMB8 Antibody (monoclonal) (M01), Clone: 1B3
AMM03954G	Leading Biology	0.1mg	EUR 484
Description: A Monoclonal antibody against Human PSMB8 (monoclonal) (M01). The antibodies are raised in Mouse and are from clone 1B3. This antibody is applicable in WB, IHC and IF, IP, E

[KO Validated] PSMB8 Rabbit pAb
A19933-100ul	Abclonal	100 ul	EUR 410

[KO Validated] PSMB8 Rabbit pAb
A19933-200ul	Abclonal	200 ul	EUR 571

[KO Validated] PSMB8 Rabbit pAb
A19933-20ul	Abclonal	20 ul	EUR 221

[KO Validated] PSMB8 Rabbit pAb
A19933-50ul	Abclonal	50 ul	EUR 287

Psmb8 ORF Vector (Rat) (pORF)
ORF074211	ABM	1.0 ug DNA	EUR 506

PSMB8 ORF Vector (Human) (pORF)
ORF028453	ABM	1.0 ug DNA	EUR 405

Psmb8 ORF Vector (Mouse) (pORF)
ORF055097	ABM	1.0 ug DNA	EUR 506

PSMB8 ELISA Kit (Human) (OKCD02695)
OKCD02695	Aviva Systems Biology	96 Wells	EUR 792
Description: Description of target: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.117 ng/mL

PSMB8 ELISA Kit (Human) (OKEH07770)
OKEH07770	Aviva Systems Biology	96 Wells	EUR 1092
Description: Description of target: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.06ng/mL

PSMB8 ELISA Kit (Dog) (OKEH03975)
OKEH03975	Aviva Systems Biology	96 Wells	EUR 844
Description: Description of target: ;Species reactivity: Dog;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.064 ng/mL

Psmb8 sgRNA CRISPR Lentivector set (Rat)
K6921501	ABM	3 x 1.0 ug	EUR 339

Psmb8 sgRNA CRISPR Lentivector set (Mouse)
K4425601	ABM	3 x 1.0 ug	EUR 339

PSMB8 sgRNA CRISPR Lentivector set (Human)
K1740301	ABM	3 x 1.0 ug	EUR 339

Human Proteasome subunit beta type-8 (PSMB8)
1-CSB-EP018886HU	Cusabio	EUR 380.00 EUR 214.00 EUR 1309.00 EUR 560.00 EUR 873.00 EUR 262.00	100ug 10ug 1MG 200ug 500ug 50ug
MW: 42.7 kDa Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Proteasome subunit beta type-8(PSMB8) expressed in E.coli

Psmb8 sgRNA CRISPR Lentivector (Rat) (Target 1)
K6921502	ABM	1.0 ug DNA	EUR 154

Psmb8 sgRNA CRISPR Lentivector (Rat) (Target 2)
K6921503	ABM	1.0 ug DNA	EUR 154

Psmb8 sgRNA CRISPR Lentivector (Rat) (Target 3)
K6921504	ABM	1.0 ug DNA	EUR 154

Psmb8 sgRNA CRISPR Lentivector (Mouse) (Target 1)
K4425602	ABM	1.0 ug DNA	EUR 154

Psmb8 sgRNA CRISPR Lentivector (Mouse) (Target 2)
K4425603	ABM	1.0 ug DNA	EUR 154

Psmb8 sgRNA CRISPR Lentivector (Mouse) (Target 3)
K4425604	ABM	1.0 ug DNA	EUR 154

PSMB8 sgRNA CRISPR Lentivector (Human) (Target 1)
K1740302	ABM	1.0 ug DNA	EUR 154

PSMB8 sgRNA CRISPR Lentivector (Human) (Target 2)
K1740303	ABM	1.0 ug DNA	EUR 154

PSMB8 sgRNA CRISPR Lentivector (Human) (Target 3)
K1740304	ABM	1.0 ug DNA	EUR 154

PSMB8 Protein Vector (Human) (pPB-C-His)
PV113810	ABM	500 ng	EUR 552

PSMB8 Protein Vector (Human) (pPB-N-His)
PV113811	ABM	500 ng	EUR 552

PSMB8 Protein Vector (Human) (pPM-C-HA)
PV113812	ABM	500 ng	EUR 552

PSMB8 Protein Vector (Human) (pPM-C-His)
PV113813	ABM	500 ng	EUR 552

PSMB8 Protein Vector (Rat) (pPB-C-His)
PV296842	ABM	500 ng	EUR 603

PSMB8 Protein Vector (Rat) (pPB-N-His)
PV296843	ABM	500 ng	EUR 603

PSMB8 Protein Vector (Rat) (pPM-C-HA)
PV296844	ABM	500 ng	EUR 603

PSMB8 Protein Vector (Rat) (pPM-C-His)
PV296845	ABM	500 ng	EUR 603

PSMB8 Protein Vector (Mouse) (pPB-C-His)
PV220386	ABM	500 ng	EUR 603

PSMB8 Protein Vector (Mouse) (pPB-N-His)
PV220387	ABM	500 ng	EUR 603

PSMB8 Protein Vector (Mouse) (pPM-C-HA)
PV220388	ABM	500 ng	EUR 603

PSMB8 Protein Vector (Mouse) (pPM-C-His)
PV220389	ABM	500 ng	EUR 603

Recombinant Human PSMB8 Protein, His, E.coli-1mg
QP13190-1mg	EnQuireBio	1mg	EUR 2757

Recombinant Human PSMB8 Protein, His, E.coli-20ug
QP13190-20ug	EnQuireBio	20ug	EUR 201

Recombinant Human PSMB8 Protein, His, E.coli-5ug
QP13190-5ug	EnQuireBio	5ug	EUR 155

Psmb8 3'UTR Luciferase Stable Cell Line
TU117173	ABM	1.0 ml	Ask for price

Psmb8 3'UTR GFP Stable Cell Line
TU167173	ABM	1.0 ml	Ask for price

Psmb8 3'UTR Luciferase Stable Cell Line
TU216964	ABM	1.0 ml	Ask for price

Psmb8 3'UTR GFP Stable Cell Line
TU266964	ABM	1.0 ml	Ask for price

PSMB8 3'UTR GFP Stable Cell Line
TU069046	ABM	1.0 ml	EUR 1394

PSMB8 3'UTR Luciferase Stable Cell Line
TU019046	ABM	1.0 ml	EUR 1394

PSMB8 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)
LV700471	ABM	1.0 ug DNA	EUR 514

PSMB8 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)
LV700475	ABM	1.0 ug DNA	EUR 514

PSMB8 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)
LV700476	ABM	1.0 ug DNA	EUR 514

Dog PSMB8/ Proteasome subunit beta type-8 ELISA Kit
E0091Do	Sunlong	1 Kit	EUR 717

Rabbit Proteasome subunit β type 8(PSMB8) ELISA kit
E04P0873-192T	B-Gene	192 tests	EUR 1270
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Proteasome subunit β type 8(PSMB8) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Proteasome subunit β type 8(PSMB8) ELISA kit
E04P0873-48	B-Gene	1 plate of 48 wells	EUR 520
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Proteasome subunit β type 8(PSMB8) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Proteasome subunit β type 8(PSMB8) ELISA kit
E04P0873-96	B-Gene	1 plate of 96 wells	EUR 685
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Proteasome subunit β type 8(PSMB8) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Proteasome subunit β type 8(PSMB8) ELISA kit
E02P0873-192T	B-Gene	192 tests	EUR 1270
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Rat Proteasome subunit β type 8(PSMB8) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Proteasome subunit β type 8(PSMB8) ELISA kit
E02P0873-48	B-Gene	1 plate of 48 wells	EUR 520
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Rat Proteasome subunit β type 8(PSMB8) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Proteasome subunit β type 8(PSMB8) ELISA kit
E02P0873-96	B-Gene	1 plate of 96 wells	EUR 685
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Rat Proteasome subunit β type 8(PSMB8) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Proteasome subunit β type 8(PSMB8) ELISA kit
E03P0873-192T	B-Gene	192 tests	EUR 1270
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Mouse Proteasome subunit β type 8(PSMB8) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.