Transcriptomes of Major Proximal Tubule Cell Culture Models
Background: Cultured cell lines are widely used for research in the physiology, pathophysiology, toxicology, and pharmacology of the renal proximal tubule. The lines that are most appropriate for a given use depend upon the genes expressed. New tools for transcriptomic profiling using RNA sequencing (RNA-Seq) make it possible to catalog expressed genes in each cell line.
Methods: Fourteen different proximal tubule cell lines, representing six species, were grown on permeable supports under conditions specific for the respective lines. RNA-Seq followed standard procedures.
Results: Transcripts expressed in cell lines variably matched transcripts selectively expressed in native proximal tubule. Opossum kidney (OK) cells displayed the highest percentage match (45% of proximal marker genes [TPM threshold =15]), with pig kidney cells (LLC-PK1) close behind (39%). Lower-percentage matches were seen for various human lines, including HK-2 (26%), and lines from rodent kidneys, such as NRK-52E (23%). Nominally, identical OK cells from different sources differed substantially in expression of proximal tubule markers. Mapping cell line transcriptomes to gene sets for various proximal tubule functions (sodium and water transport, protein transport, metabolic functions, endocrine functions) showed that different lines may be optimal for experimentally modeling each function.
An online resource (https://esbl.nhlbi.nih.gov/JBrowse/KCT/) has been created to interrogate cell line transcriptome data. Proteomic analysis of NRK-52E cells confirmed low expression of many proximal tubule marker proteins.
Conclusions: No cell line fully matched the transcriptome of native proximal tubule cells. However, some of the lines tested are suitable for the study of particular metabolic and transport processes seen in the proximal tubule.
Fibroblast-like cells change gene expression of bone remodelling markers in transwell cultures
Introduction: Periprosthetic fibroblast-like cells (PPFs) play an important role in aseptic loosening of arthroplasties. Various studies have examined PPF behavior in monolayer culture systems. However, the periprosthetic tissue is a three-dimensional (3D) mesh, which allows the cells to interact in a multidirectional way. The expression of bone remodeling markers of fibroblast-like cells in a multilayer environment changes significantly versus monolayer cultures without the addition of particles or cytokine stimulation. Gene expression of bone remodeling markers was therefore compared in fibroblast-like cells from different origins and dermal fibroblasts under transwell culture conditions versus monolayer cultures.

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti chicken secondary antibody |
K3171250-III |
Biochain |
2500 cm2 |
EUR 310 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
Mounting Medium |
4300 |
Virostat |
3 ml |
EUR 180.5 |
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein. |
Advanced Medium |
C0003-04 |
Addexbio |
RT 500 mL Bottle |
EUR 103 |
Medium 199 |
C0012-01 |
Addexbio |
RT 500 mL Bottle |
EUR 105 |
Heller's Medium |
CP014-010 |
GenDepot |
10X1L |
EUR 99 |
Heller's Medium |
CP014-500 |
GenDepot |
50L |
EUR 126 |
Hoagland's Medium |
CP015-010 |
GenDepot |
10X1L |
EUR 99 |
Hoagland's Medium |
CP015-500 |
GenDepot |
50L |
EUR 126 |
NeuroProgenitor Medium |
NM42400 |
Neuromics |
125 ml |
EUR 304 |
A Medium |
DJ1018 |
Bio Basic |
100g |
EUR 84.8 |
- Product category: Culture Media/Medium
|
M9 Medium |
SD7024 |
Bio Basic |
250g |
EUR 71.75 |
- Product category: Culture Media/Medium
|
M9CA Medium |
SD7025 |
Bio Basic |
250g |
EUR 71.75 |
- Product category: Culture Media/Medium
|
YM Medium |
SD7031 |
Bio Basic |
250g |
EUR 70.45 |
- Product category: Culture Media/Medium
|
TYGPN Medium |
SD7032 |
Bio Basic |
250g |
EUR 70.01 |
- Product category: Culture Media/Medium
|
M63 Medium |
SD7033 |
Bio Basic |
250g |
EUR 71.75 |
- Product category: Culture Media/Medium
|
50ml TC Tubes, Conical, 440 units/box |
04-5540150 |
Gentaur Genprice |
440 units/box |
EUR 71 |
GMP IL4, 50µg |
04-GMP-HU-IL4-50UG |
Gentaur Genprice |
50µg |
EUR 483 |
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques. |
rHu IL 2 , 3MIU |
04-RHIL2-02F01 |
Gentaur Genprice |
1 vial |
EUR 249 |
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated. |
rHu IL 2 , 3MIU , Lot 200908F02 |
04-RHIL2-08F02 |
Gentaur Genprice |
1 vial |
EUR 249 |
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated. |
PRE-GMP rHu GM-CSF, Molgramostim-Leukoma |
04-RHUGM-CSF-7A10 |
Gentaur Genprice |
300 µg |
EUR 385 |
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD. |
Insect Cell Medium: TNM-FH Insect Culture Medium |
ABP-MED-10001 |
Allele Biotech |
1 liter |
Ask for price |
- Product line: Cell Culture Reagents
- Brand:
|
Insect Cell Medium: Serum-Free Insect Culture Medium |
ABP-MED-10002 |
Allele Biotech |
1 liter |
Ask for price |
- Product line: Cell Culture Reagents
- Brand:
|
Insect Cell Medium: Grace?s Insect Medium (Unsupplemented) |
ABP-MED-10004 |
Allele Biotech |
1 liter |
Ask for price |
- Product line: Cell Culture Reagents
- Brand:
|
EverBrite Mounting Medium |
23001 |
Biotium |
10mL |
EUR 178 |
Description: Minimum order quantity: 1 unit of 10mL |
SuperMOUNT? Mounting Medium |
1211-20 |
Biovision |
|
EUR 201 |
Aqueous Mounting Medium |
AR1018 |
BosterBio |
10mL X5 (Enough for 800-1200 assays) |
EUR 80 |
Antifade Mounting Medium |
AR1109 |
BosterBio |
10mL (for 1000 assays) |
EUR 65 |
RPMI 1640 Medium |
abx098879-500ml |
Abbexa |
500 ml |
EUR 175 |
- Shipped within 5-10 working days.
|
MCDB 131 Medium |
CM034-050 |
GenDepot |
500ml |
EUR 85 |
MCDB 131 Medium |
CM034-300 |
GenDepot |
6x500ml |
EUR 165 |
MCDB 131 Medium |
CM034-310 |
GenDepot |
10x500ml |
EUR 219 |
MCDB 131 Medium |
CM034-320 |
GenDepot |
20x500ml |
EUR 340 |
MCDB 131 Medium |
CM034-350 |
GenDepot |
50x500ml |
EUR 585 |
Williams' Medium
E |
CM063-050 |
GenDepot |
500ml |
EUR 91 |
Williams' Medium
E |
CM063-300 |
GenDepot |
6x500ml |
EUR 266 |
Williams' Medium
E |
CM063-310 |
GenDepot |
10x500ml |
EUR 313 |
Williams' Medium
E |
CM063-320 |
GenDepot |
20x500ml |
EUR 604 |
Williams' Medium
E |
CM063-350 |
GenDepot |
50x500ml |
EUR 975 |
AddexBio Cryopreservation Medium |
C0002-20mL |
Addexbio |
RT vial |
EUR 104 |
Cereal Crops Medium |
CP003-005 |
GenDepot |
5L |
EUR 113 |
Cereal Crops Medium |
CP003-010 |
GenDepot |
10X1L |
EUR 169 |
Kao Michayluk Medium |
CP017-010 |
GenDepot |
10X1L |
EUR 99 |
Kao Michayluk Medium |
CP017-500 |
GenDepot |
50L |
EUR 126 |
Orchid Maintenance Medium |
CP037-010 |
GenDepot |
10X1L |
EUR 109 |
Orchid Maintenance Medium |
CP037-500 |
GenDepot |
50L |
EUR 138 |
Woody Plants Medium |
CP040-010 |
GenDepot |
10X1L |
EUR 101 |
Woody Plants Medium |
CP040-500 |
GenDepot |
50L |
EUR 126 |
Hepatocyte Maintenance Medium |
HM42600 |
Neuromics |
100 ml |
EUR 409 |
Neuron Growth Medium |
HNM001 |
Neuromics |
500 ml |
EUR 331 |
Sepadextran-25 Medium |
SD25M-100 |
ABTBeads |
100 g |
EUR 271 |
Sepadextran-25 Medium |
SD25M-500 |
ABTBeads |
500 g |
EUR 1036 |
Sepadextran-50 Medium |
SD50M-100 |
ABTBeads |
100 g |
EUR 344 |
Sepadextran-50 Medium |
SD50M-500 |
ABTBeads |
500 g |
EUR 1285 |
Schwann Growth Medium |
SGM001 |
Neuromics |
500 ml |
EUR 244 |
H Medium Broth |
DJ1019 |
Bio Basic |
100g |
EUR 60.88 |
- Product category: Culture Media/Broth
|
Angiotensin III (Ang III) Antibody |
20-abx101595 |
Abbexa |
-
EUR 425.00
-
EUR 133.00
-
EUR 1205.00
-
EUR 578.00
-
EUR 328.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
- Shipped within 5-15 working days.
|
Bovine Antithrombin III (AT III) Antibody |
22111-05011 |
AssayPro |
150 ug |
EUR 217 |
Angiotensin III (Ang III) Protein (OVA) |
20-abx651227 |
Abbexa |
-
EUR 258.00
-
EUR 189.00
-
EUR 578.00
-
EUR 286.00
-
EUR 230.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
- Shipped within 5-7 working days.
|
Jingzhaotoxin III |
B5763-.1 |
ApexBio |
100 ug |
EUR 601 |
Filipin III |
B6034-.5 |
ApexBio |
500 µg |
EUR 173 |
Filipin III |
B6034-1 |
ApexBio |
1 mg |
EUR 222 |
Filipin III |
B6034-5 |
ApexBio |
5 mg |
EUR 740 |
PIK-III |
B6160-25 |
ApexBio |
25 mg |
EUR 615 |
Description: IC50: 18 nM for VPS34PIK-III is a VPS34 inhibitor and is able to inhibit autophagy. |
PIK-III |
B6160-5 |
ApexBio |
5 mg |
EUR 209 |
Description: IC50: 18 nM for VPS34PIK-III is a VPS34 inhibitor and is able to inhibit autophagy. |
III Antibody |
1-CSB-PA303116LA01ECY |
Cusabio |
|
|
- Form: Liquid
- Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified |
Description: A polyclonal antibody against III. Recognizes III from Enterobacteria phage M13. This antibody is Unconjugated. Tested in the following application: ELISA |
Microcide III |
99R-103 |
Fitzgerald |
500 ml |
EUR 354 |
Description: Microcide compound for broad-spectrum antimicrobial activity against bacteria and fungi and are compatible with most formulations. |
Filipin III |
20-abx076644 |
Abbexa |
|
|
- Shipped within 5-12 working days.
|
Corynecin III |
C186-1MG |
TOKU-E |
1 mg |
EUR 154 |
Corynecin III |
C186-5MG |
TOKU-E |
5 mg |
EUR 465 |
Methods: PPFs from periprosthetic tissues (n = 12), osteoarthritic (OA) synovial fibroblast-like cells (SFs) (n = 6), and dermal fibroblasts (DFs) were cultured in monolayer (density 5.5 × 103/cm2) or multilayer cultures (density 8.5 × 105/cm2) for 10 or 21 days. Cultures were examined via histology, TRAP staining, immunohistochemistry (anti-S100a4), and quantitative real-time PCR.
Results: Fibroblast-like cells (PPFs/SFs) and dermal fibroblasts significantly increased the expression of RANKL and significantly decreased the expression of ALP, COL1A1, and OPG in multilayer cultures. PPFs and SFs in multilayer cultures further showed a higher expression of cathepsin K, MMP-13, and TNF-α. In multilayer PPF cultures, the mRNA level of TRAP was also found to be significantly increased.
Conclusion: The multilayer cultures are able to induce significant expression changes in fibroblast-like cells depending on the nature of cellular origin without the addition of any further stimulus. This system might be a useful tool to get more in vivo like results regarding fibroblast-like cell cultures.
A novel method of cell culture based on the microfluidic chip for regulation of cell density
The regulation of cell density is an important segment in microfluidic cell culture, particularly in the repeated assays. Traditionally, the consistent cell density is difficult to achieve owing to the inaccurate regulation of cell density with manual feedback. A novel cell culture method with automatic feedback is proposed for real-time regulation of cell density based on microfluidic chip in this paper. Here, an integrated microfluidic system combining cell culture, density detection and control of proliferation rate was developed.
Interdigital electrode structures (IDES) were sputtered on the microchannel for automatically to provide the real-time feedback information of impedance. The most sensitive frequency was studied to improve the detection resolution of the sensing chip. Cells were cultured on the chip surface and cell density was detected by monitoring the alternation of the impedance. The feedback controller was established by least squares support vector machines (LS-SVM). Then, the cell proliferation rate was automatically controlled using the feedback controller to achieve the desired cell density in the repeated assays.
The results show that the standard error of this method is 2.8% indicating that the method can keep consistency of cell density in the repeated assays. This study provides a basis for improving the accuracy and repeatability in the further assays of finding the optimal drug concentration. This article is protected by copyright. All rights reserved.
Effects of Oxygen and Glucose on Bone Marrow Mesenchymal Stem Cell Culture
This study determines whether the viability of mesenchymal stem cell (MSC) in vitro is most sensitive to oxygen supply, energetic substrate supply, or accumulation of lactate. Mouse unmodified (wild type (WT)) and erythropoietin (EPO) gene-modified MSC is cultured for 7 days in normoxic (21%) and anoxic conditions. WT-MSC is cultured in anoxia for 45 days in high and regular glucose media and both have similar viability when compared to their normoxic controls at 7 days. Protein production of EPO-MSC is unaffected by the absence of oxygen.
MSC doubling time and post-anoxic exposure is increased (WT: 32.3-73.3 h; EPO: 27.2-115 h). High glucose leads to a 37% increase in cell viability at 13 days and 17% at 30 days, indicating that MSC anoxic survival is affected by supply of metabolic substrate. However, after 30 days, little difference in viability is found, and at 45 days, complete cell death occurs in both the conditions. This death cannot be attributed to lack of glucose or lactate levels. MSC stemness is retained for both osteogenic and adipogenic differentiations. The absence of oxygen increases the doubling time of MSC but does not affect their viability, protein production, or differentiation capacity.
Monolayer/spheroid co-culture of cells on a PDMS well plate mediated by selective polydopamine coating
Here, we have selectively coated polydopamine (PDA) onto a polydimethylsiloxane (PDMS) well plate to enable the cell co-culture of a monolayer and spheroids in a semi-segregated manner. During the coating process, the contact between the PDA solution and PDMS well plate was limited to the outer flat surface because the strong hydrophobicity of PDMS prevented the access of the PDA solution into the concave structures. This resulted in a spatially-defined coating of PDA. The success of PDA coating was evidenced by measuring the water contact angle, observing the liquid-air interface, and via PDA-specific metallization.
This platform provides a simultaneous cell culture in both a monolayer and spheroids employing either monotypic or heterotypic cells. For the monotypic culture, mesenchymal stem cells (MSCs) were seeded over the well plate to concurrently generate the monolayer and spheroids. In the heterotypic culture, MSCs were first seeded into the wells to form spheroids. Then, human umbilical vein endothelial cells (HUVECs) were added over the flat surface of the well plate and allowed to form a monolayer. The microscopic observation and fluorescence-based cell staining confirmed the clear segregation between the monolayer and spheroids in both monotypic and heterotypic cultures. This new model could pave the way for the construction of a platform closely mimicking the physiological environment used to investigate cell-cell interactions and communications applicable for drug screening.
PSMB8 Antibody |
43134-100ul |
SAB |
100ul |
EUR 252 |
PSMB8 Antibody |
1-CSB-PA116429 |
Cusabio |
|
|
- Form: Liquid
- Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
|
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100 |
PSMB8 Antibody |
1-CSB-PA775839 |
Cusabio |
|
|
- Form: Liquid
- Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
|
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100 |
PSMB8 Antibody |
BF0434 |
Affbiotech |
200ul |
EUR 376 |
Description: PSMB8 antibody detects endogenous levels of total PSMB8. |
PSMB8 Antibody |
1-CSB-PA018886GA01HU |
Cusabio |
|
|
- Form: Liquid
- Buffer: PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20℃, Avoid freeze / thaw cycles. Antigen Affinity purified
|
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF |
PSMB8 Antibody |
1-CSB-PA018886LA01HU |
Cusabio |
|
|
- Form: Liquid
- Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified |
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200 |
PSMB8 Conjugated Antibody |
C43134 |
SAB |
100ul |
EUR 397 |
Anti-PSMB8 antibody |
STJ11100809 |
St John's Laboratory |
100 µl |
EUR 413 |
Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. |
Anti-PSMB8 antibody |
STJ29479 |
St John's Laboratory |
100 µl |
EUR 277 |
Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. |
PSMB8 siRNA |
20-abx904317 |
Abbexa |
|
|
- Shipped within 5-10 working days.
|
PSMB8 siRNA |
20-abx930156 |
Abbexa |
|
|
- Shipped within 5-10 working days.
|
PSMB8 siRNA |
20-abx930157 |
Abbexa |
|
|
- Shipped within 5-10 working days.
|
PSMB8 Antibody, HRP conjugated |
1-CSB-PA018886LB01HU |
Cusabio |
|
|
- Form: Liquid
- Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified |
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
PSMB8 Antibody, FITC conjugated |
1-CSB-PA018886LC01HU |
Cusabio |
|
|
- Form: Liquid
- Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified |
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
PSMB8 Antibody, Biotin conjugated |
1-CSB-PA018886LD01HU |
Cusabio |
|
|
- Form: Liquid
- Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified |
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
PSMB8 Blocking Peptide |
BF0434-BP |
Affbiotech |
1mg |
EUR 195 |
PSMB8 Rabbit pAb |
A7340-100ul |
Abclonal |
100 ul |
EUR 308 |
PSMB8 Rabbit pAb |
A7340-200ul |
Abclonal |
200 ul |
EUR 459 |
PSMB8 Rabbit pAb |
A7340-20ul |
Abclonal |
20 ul |
EUR 183 |
PSMB8 Rabbit pAb |
A7340-50ul |
Abclonal |
50 ul |
EUR 223 |
anti-PSMB8 (1A5) |
LF-MA30618 |
Abfrontier |
100 ul |
EUR 527 |
Description: Mouse Monoclonal to PSMB8 |
Monoclonal PSMB8 Antibody, Clone: 1A5 |
AMM02864G |
Leading Biology |
0.1ml |
EUR 528 |
Description: A Monoclonal antibody against Human PSMB8. The antibodies are raised in Mouse and are from clone 1A5. This antibody is applicable in WB and IHC, ICC, E |
Anti-PSMB8 / LMP7 Monoclonal Antibody |
M02188-2 |
BosterBio |
100ug |
EUR 397 |
Description: Rabbit Monoclonal PSMB8 / LMP7 Antibody. Validated in IP, IF, WB and tested in Human, Mouse, Rat. |
PSMB8 protein (His tag) |
80R-2608 |
Fitzgerald |
100 ug |
EUR 322 |
Description: Purified recombinant PSMB8 protein |
Rat PSMB8 shRNA Plasmid |
20-abx984734 |
Abbexa |
|
|
- Shipped within 15-20 working days.
|
Human PSMB8 shRNA Plasmid |
20-abx953843 |
Abbexa |
|
|
- Shipped within 15-20 working days.
|
Mouse PSMB8 shRNA Plasmid |
20-abx971337 |
Abbexa |
|
|
- Shipped within 15-20 working days.
|
PSMB8 Recombinant Protein (Human) |
RP085356 |
ABM |
100 ug |
Ask for price |
PSMB8 Recombinant Protein (Mouse) |
RP165287 |
ABM |
100 ug |
Ask for price |
PSMB8 Recombinant Protein (Rat) |
RP222629 |
ABM |
100 ug |
Ask for price |
Proteasome Subunit Beta Type-8 (PSMB8) Antibody |
20-abx006453 |
Abbexa |
-
EUR 411.00
-
EUR 592.00
-
EUR 182.00
-
EUR 314.00
|
-
100 ul
-
200 ul
-
20 ul
-
50 ul
|
- Shipped within 5-10 working days.
|
Proteasome Subunit Beta Type-8 (PSMB8) Antibody |
abx028154-400ul |
Abbexa |
400 ul |
EUR 523 |
- Shipped within 5-10 working days.
|
Proteasome Subunit Beta Type-8 (PSMB8) Antibody |
abx028154-80l |
Abbexa |
80 µl |
EUR 286 |
- Shipped within 5-10 working days.
|
Proteasome subunit beta type-8 (PSMB8) Antibody |
20-abx211178 |
Abbexa |
|
|
- Shipped within 5-10 working days.
|
Proteasome subunit beta type-8 (PSMB8) Antibody |
20-abx211851 |
Abbexa |
|
|
- Shipped within 5-10 working days.
|
Proteasome Subunit Beta Type-8 (PSMB8) Antibody |
20-abx114776 |
Abbexa |
|
|
- Shipped within 5-10 working days.
|
Proteasome Subunit Beta Type-8 (PSMB8) Antibody |
20-abx141396 |
Abbexa |
-
EUR 370.00
-
EUR 606.00
-
EUR 300.00
|
|
- Shipped within 5-10 working days.
|
Proteasome Subunit Beta Type-8 (PSMB8) Antibody |
abx011689-100ul |
Abbexa |
100 ul |
EUR 411 |
- Shipped within 5-10 working days.
|
Monoclonal PSMB8 Antibody (monoclonal) (M01), Clone: 1B3 |
AMM03954G |
Leading Biology |
0.1mg |
EUR 484 |
Description: A Monoclonal antibody against Human PSMB8 (monoclonal) (M01). The antibodies are raised in Mouse and are from clone 1B3. This antibody is applicable in WB, IHC and IF, IP, E |
[KO Validated] PSMB8 Rabbit pAb |
A19933-100ul |
Abclonal |
100 ul |
EUR 410 |
[KO Validated] PSMB8 Rabbit pAb |
A19933-200ul |
Abclonal |
200 ul |
EUR 571 |
[KO Validated] PSMB8 Rabbit pAb |
A19933-20ul |
Abclonal |
20 ul |
EUR 221 |
[KO Validated] PSMB8 Rabbit pAb |
A19933-50ul |
Abclonal |
50 ul |
EUR 287 |
Psmb8 ORF Vector (Rat) (pORF) |
ORF074211 |
ABM |
1.0 ug DNA |
EUR 506 |
PSMB8 ORF Vector (Human) (pORF) |
ORF028453 |
ABM |
1.0 ug DNA |
EUR 405 |
Psmb8 ORF Vector (Mouse) (pORF) |
ORF055097 |
ABM |
1.0 ug DNA |
EUR 506 |
PSMB8 ELISA Kit (Human) (OKCD02695) |
OKCD02695 |
Aviva Systems Biology |
96 Wells |
EUR 792 |
Description: Description of target: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.117 ng/mL |
PSMB8 ELISA Kit (Human) (OKEH07770) |
OKEH07770 |
Aviva Systems Biology |
96 Wells |
EUR 1092 |
Description: Description of target: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.06ng/mL |
PSMB8 ELISA Kit (Dog) (OKEH03975) |
OKEH03975 |
Aviva Systems Biology |
96 Wells |
EUR 844 |
Description: Description of target: ;Species reactivity: Dog;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.064 ng/mL |
Psmb8 sgRNA CRISPR Lentivector set (Rat) |
K6921501 |
ABM |
3 x 1.0 ug |
EUR 339 |
Psmb8 sgRNA CRISPR Lentivector set (Mouse) |
K4425601 |
ABM |
3 x 1.0 ug |
EUR 339 |
PSMB8 sgRNA CRISPR Lentivector set (Human) |
K1740301 |
ABM |
3 x 1.0 ug |
EUR 339 |
Human Proteasome subunit beta type-8 (PSMB8) |
1-CSB-EP018886HU |
Cusabio |
-
EUR 380.00
-
EUR 214.00
-
EUR 1309.00
-
EUR 560.00
-
EUR 873.00
-
EUR 262.00
|
-
100ug
-
10ug
-
1MG
-
200ug
-
500ug
-
50ug
|
- MW: 42.7 kDa
- Buffer composition: Tris-based buffer with 50% glycerol.
|
Description: Recombinant Human Proteasome subunit beta type-8(PSMB8) expressed in E.coli |
Psmb8 sgRNA CRISPR Lentivector (Rat) (Target 1) |
K6921502 |
ABM |
1.0 ug DNA |
EUR 154 |
Psmb8 sgRNA CRISPR Lentivector (Rat) (Target 2) |
K6921503 |
ABM |
1.0 ug DNA |
EUR 154 |
Psmb8 sgRNA CRISPR Lentivector (Rat) (Target 3) |
K6921504 |
ABM |
1.0 ug DNA |
EUR 154 |
Psmb8 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
K4425602 |
ABM |
1.0 ug DNA |
EUR 154 |
Psmb8 sgRNA CRISPR Lentivector (Mouse) (Target 2) |
K4425603 |
ABM |
1.0 ug DNA |
EUR 154 |
Psmb8 sgRNA CRISPR Lentivector (Mouse) (Target 3) |
K4425604 |
ABM |
1.0 ug DNA |
EUR 154 |
PSMB8 sgRNA CRISPR Lentivector (Human) (Target 1) |
K1740302 |
ABM |
1.0 ug DNA |
EUR 154 |
PSMB8 sgRNA CRISPR Lentivector (Human) (Target 2) |
K1740303 |
ABM |
1.0 ug DNA |
EUR 154 |
PSMB8 sgRNA CRISPR Lentivector (Human) (Target 3) |
K1740304 |
ABM |
1.0 ug DNA |
EUR 154 |
PSMB8 Protein Vector (Human) (pPB-C-His) |
PV113810 |
ABM |
500 ng |
EUR 552 |
PSMB8 Protein Vector (Human) (pPB-N-His) |
PV113811 |
ABM |
500 ng |
EUR 552 |
PSMB8 Protein Vector (Human) (pPM-C-HA) |
PV113812 |
ABM |
500 ng |
EUR 552 |
PSMB8 Protein Vector (Human) (pPM-C-His) |
PV113813 |
ABM |
500 ng |
EUR 552 |
PSMB8 Protein Vector (Rat) (pPB-C-His) |
PV296842 |
ABM |
500 ng |
EUR 603 |
PSMB8 Protein Vector (Rat) (pPB-N-His) |
PV296843 |
ABM |
500 ng |
EUR 603 |
PSMB8 Protein Vector (Rat) (pPM-C-HA) |
PV296844 |
ABM |
500 ng |
EUR 603 |
PSMB8 Protein Vector (Rat) (pPM-C-His) |
PV296845 |
ABM |
500 ng |
EUR 603 |
PSMB8 Protein Vector (Mouse) (pPB-C-His) |
PV220386 |
ABM |
500 ng |
EUR 603 |
PSMB8 Protein Vector (Mouse) (pPB-N-His) |
PV220387 |
ABM |
500 ng |
EUR 603 |
PSMB8 Protein Vector (Mouse) (pPM-C-HA) |
PV220388 |
ABM |
500 ng |
EUR 603 |
PSMB8 Protein Vector (Mouse) (pPM-C-His) |
PV220389 |
ABM |
500 ng |
EUR 603 |
Recombinant Human PSMB8 Protein, His, E.coli-1mg |
QP13190-1mg |
EnQuireBio |
1mg |
EUR 2757 |
Recombinant Human PSMB8 Protein, His, E.coli-20ug |
QP13190-20ug |
EnQuireBio |
20ug |
EUR 201 |
Recombinant Human PSMB8 Protein, His, E.coli-5ug |
QP13190-5ug |
EnQuireBio |
5ug |
EUR 155 |
Psmb8 3'UTR Luciferase Stable Cell Line |
TU117173 |
ABM |
1.0 ml |
Ask for price |
Psmb8 3'UTR GFP Stable Cell Line |
TU167173 |
ABM |
1.0 ml |
Ask for price |
Psmb8 3'UTR Luciferase Stable Cell Line |
TU216964 |
ABM |
1.0 ml |
Ask for price |
Psmb8 3'UTR GFP Stable Cell Line |
TU266964 |
ABM |
1.0 ml |
Ask for price |
PSMB8 3'UTR GFP Stable Cell Line |
TU069046 |
ABM |
1.0 ml |
EUR 1394 |
PSMB8 3'UTR Luciferase Stable Cell Line |
TU019046 |
ABM |
1.0 ml |
EUR 1394 |
PSMB8 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV) |
LV700471 |
ABM |
1.0 ug DNA |
EUR 514 |
PSMB8 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC) |
LV700475 |
ABM |
1.0 ug DNA |
EUR 514 |
PSMB8 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a) |
LV700476 |
ABM |
1.0 ug DNA |
EUR 514 |
Dog PSMB8/ Proteasome subunit beta type-8 ELISA Kit |
E0091Do |
Sunlong |
1 Kit |
EUR 717 |
Rabbit Proteasome subunit β type 8(PSMB8) ELISA kit |
E04P0873-192T |
B-Gene |
192 tests |
EUR 1270 |
- Kit contents: 1. MICROTITER PLATE * 1
2. ENZYME CONJUGATE*1 vial
3. STANDARD A*1 vial
4. STANDARD B*1 vial
5. STANDARD C*1 vial
6. STANDARD D*1 vial
7. STANDARD E*1 vial
8. STANDARD F*1 vial
9. SUBSTRATE A*1 vial
10. SUBSTRATE B*1 vial
11. STOP SOLUT
- Show more
|
Description: A competitive ELISA for quantitative measurement of Rabbit Proteasome subunit β type 8(PSMB8) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Proteasome subunit β type 8(PSMB8) ELISA kit |
E04P0873-48 |
B-Gene |
1 plate of 48 wells |
EUR 520 |
- Kit contents: 1. MICROTITER PLATE * 1
2. ENZYME CONJUGATE*1 vial
3. STANDARD A*1 vial
4. STANDARD B*1 vial
5. STANDARD C*1 vial
6. STANDARD D*1 vial
7. STANDARD E*1 vial
8. STANDARD F*1 vial
9. SUBSTRATE A*1 vial
10. SUBSTRATE B*1 vial
11. STOP SOLUT
- Show more
|
Description: A competitive ELISA for quantitative measurement of Rabbit Proteasome subunit β type 8(PSMB8) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Proteasome subunit β type 8(PSMB8) ELISA kit |
E04P0873-96 |
B-Gene |
1 plate of 96 wells |
EUR 685 |
- Kit contents: 1. MICROTITER PLATE * 1
2. ENZYME CONJUGATE*1 vial
3. STANDARD A*1 vial
4. STANDARD B*1 vial
5. STANDARD C*1 vial
6. STANDARD D*1 vial
7. STANDARD E*1 vial
8. STANDARD F*1 vial
9. SUBSTRATE A*1 vial
10. SUBSTRATE B*1 vial
11. STOP SOLUT
- Show more
|
Description: A competitive ELISA for quantitative measurement of Rabbit Proteasome subunit β type 8(PSMB8) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Proteasome subunit β type 8(PSMB8) ELISA kit |
E02P0873-192T |
B-Gene |
192 tests |
EUR 1270 |
- Kit contents: 1. MICROTITER PLATE * 1
2. ENZYME CONJUGATE*1 vial
3. STANDARD A*1 vial
4. STANDARD B*1 vial
5. STANDARD C*1 vial
6. STANDARD D*1 vial
7. STANDARD E*1 vial
8. STANDARD F*1 vial
9. SUBSTRATE A*1 vial
10. SUBSTRATE B*1 vial
11. STOP SOLUT
- Show more
|
Description: A competitive ELISA for quantitative measurement of Rat Proteasome subunit β type 8(PSMB8) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Proteasome subunit β type 8(PSMB8) ELISA kit |
E02P0873-48 |
B-Gene |
1 plate of 48 wells |
EUR 520 |
- Kit contents: 1. MICROTITER PLATE * 1
2. ENZYME CONJUGATE*1 vial
3. STANDARD A*1 vial
4. STANDARD B*1 vial
5. STANDARD C*1 vial
6. STANDARD D*1 vial
7. STANDARD E*1 vial
8. STANDARD F*1 vial
9. SUBSTRATE A*1 vial
10. SUBSTRATE B*1 vial
11. STOP SOLUT
- Show more
|
Description: A competitive ELISA for quantitative measurement of Rat Proteasome subunit β type 8(PSMB8) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Proteasome subunit β type 8(PSMB8) ELISA kit |
E02P0873-96 |
B-Gene |
1 plate of 96 wells |
EUR 685 |
- Kit contents: 1. MICROTITER PLATE * 1
2. ENZYME CONJUGATE*1 vial
3. STANDARD A*1 vial
4. STANDARD B*1 vial
5. STANDARD C*1 vial
6. STANDARD D*1 vial
7. STANDARD E*1 vial
8. STANDARD F*1 vial
9. SUBSTRATE A*1 vial
10. SUBSTRATE B*1 vial
11. STOP SOLUT
- Show more
|
Description: A competitive ELISA for quantitative measurement of Rat Proteasome subunit β type 8(PSMB8) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Proteasome subunit β type 8(PSMB8) ELISA kit |
E03P0873-192T |
B-Gene |
192 tests |
EUR 1270 |
- Kit contents: 1. MICROTITER PLATE * 1
2. ENZYME CONJUGATE*1 vial
3. STANDARD A*1 vial
4. STANDARD B*1 vial
5. STANDARD C*1 vial
6. STANDARD D*1 vial
7. STANDARD E*1 vial
8. STANDARD F*1 vial
9. SUBSTRATE A*1 vial
10. SUBSTRATE B*1 vial
11. STOP SOLUT
- Show more
|
Description: A competitive ELISA for quantitative measurement of Mouse Proteasome subunit β type 8(PSMB8) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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