Transcriptomes of Major Proximal Tubule Cell Culture Models

eaaci2015

Transcriptomes of Major Proximal Tubule Cell Culture Models

Background: Cultured cell lines are widely used for research in the physiology, pathophysiology, toxicology, and pharmacology of the renal proximal tubule. The lines that are most appropriate for a given use depend upon the genes expressed. New tools for transcriptomic profiling using RNA sequencing (RNA-Seq) make it possible to catalog expressed genes in each cell line.
Methods: Fourteen different proximal tubule cell lines, representing six species, were grown on permeable supports under conditions specific for the respective lines. RNA-Seq followed standard procedures.
Results: Transcripts expressed in cell lines variably matched transcripts selectively expressed in native proximal tubule. Opossum kidney (OK) cells displayed the highest percentage match (45% of proximal marker genes [TPM threshold =15]), with pig kidney cells (LLC-PK1) close behind (39%). Lower-percentage matches were seen for various human lines, including HK-2 (26%), and lines from rodent kidneys, such as NRK-52E (23%). Nominally, identical OK cells from different sources differed substantially in expression of proximal tubule markers. Mapping cell line transcriptomes to gene sets for various proximal tubule functions (sodium and water transport, protein transport, metabolic functions, endocrine functions) showed that different lines may be optimal for experimentally modeling each function.
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An online resource (https://esbl.nhlbi.nih.gov/JBrowse/KCT/) has been created to interrogate cell line transcriptome data. Proteomic analysis of NRK-52E cells confirmed low expression of many proximal tubule marker proteins.
Conclusions: No cell line fully matched the transcriptome of native proximal tubule cells. However, some of the lines tested are suitable for the study of particular metabolic and transport processes seen in the proximal tubule.

Fibroblast-like cells change gene expression of bone remodelling markers in transwell cultures

Introduction: Periprosthetic fibroblast-like cells (PPFs) play an important role in aseptic loosening of arthroplasties. Various studies have examined PPF behavior in monolayer culture systems. However, the periprosthetic tissue is a three-dimensional (3D) mesh, which allows the cells to interact in a multidirectional way. The expression of bone remodeling markers of fibroblast-like cells in a multilayer environment changes significantly versus monolayer cultures without the addition of particles or cytokine stimulation. Gene expression of bone remodeling markers was therefore compared in fibroblast-like cells from different origins and dermal fibroblasts under transwell culture conditions versus monolayer cultures.
eaaci2015

Prigrow X.1 Medium

TM010 1 Kit
EUR 385

Prigrow XII Medium

TM012 500ml
EUR 125

Prigrow XIV Medium

TM014 500 ml
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Prigrow VII Medium

TM017 500ml
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Prigrow XIII Medium

TM013 500 ml
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Prigrow VIII Medium

TM018 500ml
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Prigrow XVIII Medium

TM039 500 ml
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EXS III Basal Medium

30630164-1 25 L
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EXS III Basal Medium

30630164-2 50 L
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Artificial Seawater Nutrient Medium (III)

PT153-10X1L 1 unit
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Artificial Seawater Nutrient Medium (III)

PT153-25L 1 unit
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Description: Artificial Seawater Nutrient Medium (III)

Artificial Seawater Nutrient Medium (III)

PT153-5L 1 unit
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Description: Artificial Seawater Nutrient Medium (III)

Hosta Rooting Medium Stage III

30630167-3 25 L
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Hosta Rooting Medium Stage III

30630167-4 50 L
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Gentodenz

19-DENZ-500 500 g
EUR 416

EXS III Basal Salt Medium, w/ Adenine

30630163-1 25 L
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EXS III Basal Salt Medium, w/ Adenine

30630163-2 50 L
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KATO III [KATO 3] Cells Complete Medium

CM-0372-125mL4 125 mL×4
EUR 108
Description: Complete Growth Medium

KATO III [KATO 3] Cells Complete Medium

CM-0372 125mL×4
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Description: Cell lines complete growth medium

KATO III [KATO 3] Cells Complete Medium

MBS2568801-4x125mL 4x125mL
EUR 160

Rye Agar A

765-M1854-500G 500 g
EUR 82

Rye Agar B

765-M1855-500G 500 g
EUR 93

Porcine Parvovirus Antibody Elisa Test Kit

767-LSY-30009 192 Wells/kit
EUR 382

QuantiChrom Hemoglobin Assay Kit

65-DIHB-250 250 tests
EUR 473

Malachite Green Phosphate Assay kit

65-POMG-25H 2500 tests
EUR 333

50ml TC Tubes, Conical, 440 units/box

04-5540150 440 units/box
EUR 85.2

GMP IL4, 50µg

04-GMP-HU-IL4-50UG 50µg
EUR 579.6
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.

SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE

04-GSB
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Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You!

rHu IL 2 , 3MIU

04-RHIL2-02F01 1 vial
EUR 298.8
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

rHu IL 2 , 3MIU , Lot 200908F02

04-RHIL2-08F02 1 vial
EUR 298.8
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

PRE-GMP rHu GM-CSF, Molgramostim-Leukoma

04-RHUGM-CSF-7A10 300 µg
EUR 462
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD.

Mouse Anti TNP Immunotoxicity

198-TNPG-1 100 µL
EUR 469.2

Exo-Check Exosome Antibody Array

322-EXO-FBS-50A-1 100 µg
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1-Step Polymorphs, Human Cell Separation

71-AN221725 1
EUR 238.8

Monkeypox Virus Real Time PCR Kit

ZD-0076-01 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit

ZD-0076-02 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

GMP Recombinant Human Interleukin-4 (IL-4)

GMPhuIL-4-50ug 50ug
EUR 528

Pringsheim's Medium

M698-100G 1 unit
EUR 23.3
Description: Pringsheim's Medium

BAT Medium (Alicyclobacillus Medium)

M1561-500G 1 unit
EUR 43.27
Description: BAT Medium (Alicyclobacillus Medium)

Cooked M Medium (R.C. Medium)

M149-100G 1 unit
EUR 27.32
Description: Cooked M Medium (R.C. Medium)

Cooked M Medium (R.C. Medium)

M149-500G 1 unit
EUR 130.49
Description: Cooked M Medium (R.C. Medium)

MSC Prime Medium, 25x

TBS8022-01 1 mL
EUR 76

MSC Prime Medium, 25x

TBS8022-05 5 mL
EUR 268

MSC Prime Medium, 25x

TBS8022-25 25 mL
EUR 968

Lowenstein - Jensen Medium (L.J. Medium)

MM162-100G 1 unit
EUR 12.1
Description: Lowenstein - Jensen Medium (L.J. Medium)

Lowenstein - Jensen Medium (L.J. Medium)

MM162-500G 1 unit
EUR 34.85
Description: Lowenstein - Jensen Medium (L.J. Medium)

ATCC Medium 1754: PETC Medium (Tetra Pac

M2104-500G 1 unit
EUR 63.72
Description: ATCC Medium 1754: PETC Medium (Tetra Pac

General Freeze Medium

PB180436-10mL10 10 mL×10
EUR 158
Description: Supplements & Reagents

General Freeze Medium

PB180436-10mL5 10 mL×5
EUR 98
Description: Supplements & Reagents

General Freeze Medium

MBS2567647-10x10mL 10x10mL
EUR 195

General Freeze Medium

MBS2567647-5x10mL 5x10mL
EUR 155

Human iPS Growth Medium (PSGro Medium)

TBS8023 500 mL
EUR 189

Rat Cartilage Growth Medium PrimaCell: Normal Articular Cartilage Growth Medium

9-25023 5 x 100 ml Ask for price

General Freezing Medium

PB180436 10mL×5
EUR 98
Description: Others

Medium 199

C0012-01 RT 500 mL Bottle
EUR 34

Medium 199

MBS2557648-10L 10L
EUR 165

Medium 199

MBS2557648-50L 50L
EUR 345

Medium 199

MBS2557648-5L 5L
EUR 150

Medium 199

MBS2557648-5x50L 5x50L
EUR 1060

Human Cartilage Growth Medium PrimaCell: Normal Articular Cartilage Growth Medium

9-46043 5 x 100 ml Ask for price

HS Medium (Hydrosulphite of Sodium Medium)

77707 100 Gms
EUR 7.7
Description: Part D

HS Medium (Hydrosulphite of Sodium Medium)

77707-1 500 Gms
EUR 25.66
Description: Part D

SIM Medium (Sulfite Indole Motility Medium)

49810 100 Gms
EUR 6.84
Description: Part D

SIM Medium (Sulfite Indole Motility Medium)

49810-1 500 Gms
EUR 27.37
Description: Part D

Listeria Enrichment Medium Base (UVM Medium)

28685 100 Gms
EUR 11.97
Description: Part D

Listeria Enrichment Medium Base (UVM Medium)

28685-1 500 Gms
EUR 54.74
Description: Part D

MlO Medium (Motility Indole Ornithine Medium)

88736 100 Gms
EUR 7.7
Description: Part D

MlO Medium (Motility Indole Ornithine Medium)

88736-1 500 Gms
EUR 31.64
Description: Part D

Phyto Proteose Agar Medium Base 
(KB Medium)

PHM008-100G 1 unit
EUR 13.81
Description: Phyto Proteose Agar Medium Base 
(KB Medium)

Phyto Proteose Agar Medium Base 
(KB Medium)

PHM008-500G 1 unit
EUR 60.56
Description: Phyto Proteose Agar Medium Base 
(KB Medium)

MIU Medium Base (Motility Indole Urea Medium)

16309 100 Gms
EUR 9.41
Description: Part D

MIU Medium Base (Motility Indole Urea Medium)

16309-1 500 Gms
EUR 35.07
Description: Part D

EC MEDIUM

E05-100-10kg 10 kg
EUR 976.8

EC MEDIUM

E05-100-2Kg 2 Kg
EUR 259.2

EC MEDIUM

E05-100-500g 500 g
EUR 114

DC MEDIUM

D04-117-10kg 10 kg
EUR 1894.8

DC MEDIUM

D04-117-2kg 2kg
EUR 458.4

DC MEDIUM

D04-117-500g 500 g
EUR 168

A Medium

DJ1018 100g
EUR 101.76

VP Medium

M662-500G 1 unit
EUR 52.25
Description: VP Medium

E.T. Medium

M854-500G 1 unit
EUR 36.34
Description: E.T. Medium

E.T. Medium

M854-5KG 1 unit
EUR 355.04
Description: E.T. Medium

HS Medium

M245-500G 1 unit
EUR 34.73
Description: HS Medium

X6 MEDIUM

X8458 10L
EUR 30.71

M9 Medium

SD7024 250g
EUR 86.1

YM Medium

SD7031 250g
EUR 84.54

LB Medium

TBS8056 1L
EUR 36

OF Medium - 500G

MED1664 500G
EUR 89.1

NLN Medium

30630102-2 25 L
EUR 19.28

NLN Medium

30630102-3 50 L
EUR 39.2

TAP Medium

30639002-1 100 g
EUR 48.32

TAP Medium

30639002-2 500 g
EUR 200.66

RWC Medium

M500-100G 1 unit
EUR 15.93
Description: RWC Medium

RWC Medium

M500-500G 1 unit
EUR 43.27
Description: RWC Medium

PNY Medium

M835-100G 1 unit
EUR 34.17
Description: PNY Medium

PNY Medium

M835-500G 1 unit
EUR 103.52
Description: PNY Medium

SIM Medium

M181-100G 1 unit
EUR 12.27
Description: SIM Medium

SIM Medium

M181-500G 1 unit
EUR 35.37
Description: SIM Medium

AKI Medium

M1879-500G 1 unit
EUR 36.24
Description: AKI Medium

MIU Medium

LQ092-25X10ML 1 unit
EUR 14.98
Description: MIU Medium

MIU Medium

LQ092-50X10ML 1 unit
EUR 24.33
Description: MIU Medium

SIM Medium

GM181-500G 1 unit
EUR 40.65
Description: SIM Medium

YEP MEDIUM

Y889 1L
EUR 75.28

HLP MEDIUM

H08-107-10kg 10 kg
EUR 2733.6

HLP MEDIUM

H08-107-2Kg 2 Kg
EUR 640.8

HLP MEDIUM

H08-107-500g 500 g
EUR 218.4

SIM MEDIUM

S19-110-10kg 10 kg
EUR 1225.2

SIM MEDIUM

S19-110-2kg 2kg
EUR 313.2

SIM MEDIUM

S19-110-500g 500 g
EUR 128.4

SOB MEDIUM

S19-124-10kg 10 kg
EUR 1158

SOB MEDIUM

S19-124-2kg 2kg
EUR 298.8

SOB MEDIUM

S19-124-500g 500 g
EUR 124.8

M63 Medium

SD7033 250g
EUR 86.1

MSC Medium

TBS8021 500 mL
EUR 245

SOB Medium

TBS8057 1L
EUR 45

SOC Medium

TBS8058 100 mL
EUR 35

RM-01 Refeed Medium (zero-Serum Refeed medium)

10-310100 100 mL Ask for price
Description: A defined medium with EMEM with EBSS, and Glutamine. Sterile.

VALI Medium

0450 500 ml
EUR 177.1

BEGM Medium

0451 500 ml
EUR 89.6

TM4G Medium

30630177-2 25 L
EUR 22.1

TM4G Medium

30630177-3 50 L
EUR 41.14

Burk's Medium

M707-500G 1 unit
EUR 41.14
Description: Burk's Medium

M9CA Medium

68957 100 Gms
EUR 21.38
Description: Part D

M9CA Medium

68957-1 500 Gms
EUR 86.38
Description: Part D

mD5A MEDIUM

M5516 10L
EUR 258.64

M9CA Medium

SD7025 250g
EUR 86.1

Insect Cell Medium: TNM-FH Insect Culture Medium

ABP-MED-10001 1 liter Ask for price

Aseptic Commissioning Medium (Aseptic Packing Line Medium)

15577 100 Gms
EUR 9.41
Description: Part D

Aseptic Commissioning Medium (Aseptic Packing Line Medium)

15577-1 500 Gms
EUR 34.21
Description: Part D

White's Medium

30630124-3 25 L
EUR 31.67

White's Medium

30630124-4 50 L
EUR 43.46

Thiol Medium

M852-500G 1 unit
EUR 42.8
Description: Thiol Medium

Craig's Medium

M974-500G 1 unit
EUR 41.3
Description: Craig's Medium

Wurtz Medium

M1065-500G 1 unit
EUR 44.15
Description: Wurtz Medium

Kaper's Medium

M1169-500G 1 unit
EUR 51.41
Description: Kaper's Medium
Methods: PPFs from periprosthetic tissues (n = 12), osteoarthritic (OA) synovial fibroblast-like cells (SFs) (n = 6), and dermal fibroblasts (DFs) were cultured in monolayer (density 5.5 × 103/cm2) or multilayer cultures (density 8.5 × 105/cm2) for 10 or 21 days. Cultures were examined via histology, TRAP staining, immunohistochemistry (anti-S100a4), and quantitative real-time PCR.
Results: Fibroblast-like cells (PPFs/SFs) and dermal fibroblasts significantly increased the expression of RANKL and significantly decreased the expression of ALP, COL1A1, and OPG in multilayer cultures. PPFs and SFs in multilayer cultures further showed a higher expression of cathepsin K, MMP-13, and TNF-α. In multilayer PPF cultures, the mRNA level of TRAP was also found to be significantly increased.
Conclusion: The multilayer cultures are able to induce significant expression changes in fibroblast-like cells depending on the nature of cellular origin without the addition of any further stimulus. This system might be a useful tool to get more in vivo like results regarding fibroblast-like cell cultures.

A novel method of cell culture based on the microfluidic chip for regulation of cell density

 

The regulation of cell density is an important segment in microfluidic cell culture, particularly in the repeated assays. Traditionally, the consistent cell density is difficult to achieve owing to the inaccurate regulation of cell density with manual feedback. A novel cell culture method with automatic feedback is proposed for real-time regulation of cell density based on microfluidic chip in this paper. Here, an integrated microfluidic system combining cell culture, density detection and control of proliferation rate was developed.

 

Interdigital electrode structures (IDES) were sputtered on the microchannel for automatically to provide the real-time feedback information of impedance. The most sensitive frequency was studied to improve the detection resolution of the sensing chip. Cells were cultured on the chip surface and cell density was detected by monitoring the alternation of the impedance. The feedback controller was established by least squares support vector machines (LS-SVM). Then, the cell proliferation rate was automatically controlled using the feedback controller to achieve the desired cell density in the repeated assays, parafina.

 

The results show that the standard error of this method is 2.8% indicating that the method can keep consistency of cell density in the repeated assays. This study provides a basis for improving the accuracy and repeatability in the further assays of finding the optimal drug concentration. This article is protected by copyright. All rights reserved to Gentaur.

 

Effects of Oxygen and Glucose on Bone Marrow Mesenchymal Stem Cell Culture

This study determines whether the viability of mesenchymal stem cell (MSC) in vitro is most sensitive to oxygen supply, energetic substrate supply, or accumulation of lactate. Mouse unmodified (wild type (WT)) and erythropoietin (EPO) gene-modified MSC is cultured for 7 days in normoxic (21%) and anoxic conditions. WT-MSC is cultured in anoxia for 45 days in high and regular glucose media and both have similar viability when compared to their normoxic controls at 7 days. Protein production of EPO-MSC is unaffected by the absence of oxygen.
MSC doubling time and post-anoxic exposure is increased (WT: 32.3-73.3 h; EPO: 27.2-115 h). High glucose leads to a 37% increase in cell viability at 13 days and 17% at 30 days, indicating that MSC anoxic survival is affected by supply of metabolic substrate. However, after 30 days, little difference in viability is found, and at 45 days, complete cell death occurs in both the conditions. This death cannot be attributed to lack of glucose or lactate levels. MSC stemness is retained for both osteogenic and adipogenic differentiations. The absence of oxygen increases the doubling time of MSC but does not affect their viability, protein production, or differentiation capacity.

Monolayer/spheroid co-culture of cells on a PDMS well plate mediated by selective polydopamine coating

Here, we have selectively coated polydopamine (PDA) onto a polydimethylsiloxane (PDMS) well plate to enable the cell co-culture of a monolayer and spheroids in a semi-segregated manner. During the coating process, the contact between the PDA solution and PDMS well plate was limited to the outer flat surface because the strong hydrophobicity of PDMS prevented the access of the PDA solution into the concave structures. This resulted in a spatially-defined coating of PDA. The success of PDA coating was evidenced by measuring the water contact angle, observing the liquid-air interface, and via PDA-specific metallization.
This platform provides a simultaneous cell culture in both a monolayer and spheroids employing either monotypic or heterotypic cells. For the monotypic culture, mesenchymal stem cells (MSCs) were seeded over the well plate to concurrently generate the monolayer and spheroids. In the heterotypic culture, MSCs were first seeded into the wells to form spheroids. Then, human umbilical vein endothelial cells (HUVECs) were added over the flat surface of the well plate and allowed to form a monolayer. The microscopic observation and fluorescence-based cell staining confirmed the clear segregation between the monolayer and spheroids in both monotypic and heterotypic cultures. This new model could pave the way for the construction of a platform closely mimicking the physiological environment used to investigate cell-cell interactions and communications applicable for drug screening.
PSMB8 Antibody
1-CSB-PA018886GA01HU
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  • 150ul
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Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF
PSMB8 Antibody
1-CSB-PA018886LA01HU
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  • 100ul
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Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200
PSMB8 Antibody
E94054 100μg
EUR 255
Description: Available in various conjugation types.
PSMB8 Antibody
E97340 100ul
EUR 255
Description: Available in various conjugation types.
PSMB8 Antibody
E314816 100ug/200ul
EUR 295
Description: Available in various conjugation types.
PSMB8 antibody
70R-13550 100 ul
EUR 550
Description: Affinity purified Rabbit polyclonal PSMB8 antibody
PSMB8 antibody
70R-19589 50 ul
EUR 289
Description: Rabbit polyclonal PSMB8 antibody
PSMB8 Antibody
1-CSB-PA116429
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  • 100ul
  • 50ul
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100
PSMB8 antibody
BF0434 100ug
EUR 355
PSMB8 Antibody
MBS7113266-005mL 0.05mL
EUR 190
PSMB8 Antibody
MBS7113266-01mL 0.1mL
EUR 270
PSMB8 Antibody
MBS7113266-5x01mL 5x0.1mL
EUR 1205
PSMB8 Antibody
MBS7129229-005mL 0.05mL
EUR 190
PSMB8 Antibody
MBS7129229-01mL 0.1mL
EUR 270
PSMB8 Antibody
MBS7129229-5x01mL 5x0.1mL
EUR 1205
PSMB8 Antibody
MBS7129230-005mL 0.05mL
EUR 190
PSMB8 Antibody
MBS7129230-01mL 0.1mL
EUR 270
PSMB8 Antibody
MBS7129230-5x01mL 5x0.1mL
EUR 1205
PSMB8 antibody
MBS9405580-01mL 0.1mL
EUR 420
PSMB8 antibody
MBS9405580-5x01mL 5x0.1mL
EUR 1740
PSMB8 Antibody
MBS8500013-01mg 0.1mg
EUR 325
PSMB8 Antibody
MBS8500013-01mLAF405L 0.1mL(AF405L)
EUR 565
PSMB8 Antibody
MBS8500013-01mLAF405S 0.1mL(AF405S)
EUR 565
PSMB8 Antibody
MBS8500013-01mLAF610 0.1mL(AF610)
EUR 565
PSMB8 Antibody
MBS8500013-01mLAF635 0.1mL(AF635)
EUR 565
PSMB8 Antibody
MBS5312424-01mL 0.1mL
EUR 1070
PSMB8 Antibody
MBS5312424-5x01mL 5x0.1mL
EUR 4655
PSMB8 Antibody
MBS9416351-01mL 0.1mL
EUR 305
PSMB8 Antibody
MBS9416351-5x01mL 5x0.1mL
EUR 1230
PSMB8 Antibody
BF0434-100ul 100ul
EUR 210
Description: WB,IHC,IF/ICC,ELISA
PSMB8 Antibody
BF0434-200ul 200ul Ask for price
Description: WB,IHC,IF/ICC,ELISA
PSMB8 Antibody
BF0434-50ul 50ul
EUR 150
Description: WB,IHC,IF/ICC,ELISA
PSMB8 Conjugated Antibody
C43134 100ul
EUR 476.4
PSMB8 Polyclonal Antibody
E-AB-92394-120uL 120uL
EUR 320
Description: Unconjugated
PSMB8 Polyclonal Antibody
E-AB-92394-200uL 200uL
EUR 530
Description: Unconjugated
PSMB8 Polyclonal Antibody
E-AB-92394-60uL 60uL
EUR 200
Description: Unconjugated
PSMB8 Polyclonal Antibody
E-AB-92394-each each Ask for price
Description: Unconjugated
PSMB8 Polyclonal Antibody
A73468 50 μl
EUR 557
PSMB8 Polyclonal Antibody
E-AB-61655-120uL 120uL
EUR 320
Description: Unconjugated
PSMB8 Polyclonal Antibody
E-AB-61655-200uL 200uL
EUR 530
Description: Unconjugated
PSMB8 Polyclonal Antibody
E-AB-61655-60uL 60uL
EUR 200
Description: Unconjugated
PSMB8 Polyclonal Antibody
E-AB-61655-each each Ask for price
Description: Unconjugated
PSMB8 Polyclonal Antibody
MBS2538339-006mL 0.06mL
EUR 190
PSMB8 Polyclonal Antibody
MBS2538339-012mL 0.12mL
EUR 265
PSMB8 Polyclonal Antibody
MBS2538339-02mL 0.2mL
EUR 415
PSMB8 Polyclonal Antibody
MBS2538339-5x02mL 5x0.2mL
EUR 1835
PSMB8 Polyclonal Antibody
MBS2527805-002mL 0.02mL
EUR 140
PSMB8 Polyclonal Antibody
MBS2527805-006mL 0.06mL
EUR 180
PSMB8 Polyclonal Antibody
MBS2527805-012mL 0.12mL
EUR 260
PSMB8 Polyclonal Antibody
MBS2527805-02mL 0.2mL
EUR 405
PSMB8 Polyclonal Antibody
MBS2527805-5x02mL 5x0.2mL
EUR 1725
PSMB8 Polyclonal Antibody
MBS2528965-002mL 0.02mL
EUR 140
PSMB8 Polyclonal Antibody
MBS2528965-006mL 0.06mL
EUR 180
PSMB8 Polyclonal Antibody
MBS2528965-012mL 0.12mL
EUR 260
PSMB8 Polyclonal Antibody
MBS2528965-02mL 0.2mL
EUR 405
PSMB8 Polyclonal Antibody
MBS2528965-5x02mL 5x0.2mL
EUR 1725
PSMB8 Conjugated Antibody
MBS9451453-01mLAF350 0.1mL(AF350)
EUR 480
PSMB8 Conjugated Antibody
MBS9451453-01mLAF405 0.1mL(AF405)
EUR 480
PSMB8 Conjugated Antibody
MBS9451453-01mLAF488 0.1mL(AF488)
EUR 480
PSMB8 Conjugated Antibody
MBS9451453-01mLAF555 0.1mL(AF555)
EUR 480
PSMB8 Conjugated Antibody
MBS9451453-01mLBiotin 0.1mL(Biotin)
EUR 480
PSMB8 Polyclonal Antibody
RD84433A-120uL 120μL
EUR 360
Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.
PSMB8 Polyclonal Antibody
RD84433A-200uL 200μL
EUR 630
Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.
PSMB8 Polyclonal Antibody
RD84433A-60uL 60μL
EUR 204
Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.
PSMB8 Antibody (N-term)
MBS9213569-008mL 0.08mL
EUR 210
PSMB8 Antibody (N-term)
MBS9213569-04mL 0.4mL
EUR 430
PSMB8 Antibody (N-term)
MBS9213569-5x04mL 5x0.4mL
EUR 1910
PSMB8 Antibody, HRP conjugated
1-CSB-PA018886LB01HU
  • Ask for price
  • Ask for price
  • 100ul
  • 50ul
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
PSMB8 Antibody, HRP Conjugated
MBS7113267-005mL 0.05mL
EUR 190
PSMB8 Antibody, HRP Conjugated
MBS7113267-01mL 0.1mL
EUR 270
PSMB8 Antibody, HRP Conjugated
MBS7113267-5x01mL 5x0.1mL
EUR 1205
PSMB8 Antibody, FITC conjugated
1-CSB-PA018886LC01HU
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  • Ask for price
  • 100ul
  • 50ul
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA
OACD05288-1ML - PSMB8 Antibody
OACD05288-1ML 1ml
EUR 649
OACD05289-1ML - PSMB8 Antibody
OACD05289-1ML 1ml
EUR 659
OACD05290-1ML - PSMB8 Antibody
OACD05290-1ML 1ml
EUR 639
PSMB8 Antibody, FITC Conjugated
MBS7113268-005mL 0.05mL
EUR 190
PSMB8 Antibody, FITC Conjugated
MBS7113268-01mL 0.1mL
EUR 270
PSMB8 Antibody, FITC Conjugated
MBS7113268-5x01mL 5x0.1mL
EUR 1205
OAAN02231-50UL - PSMB8 Antibody
OAAN02231-50UL 50ul
EUR 149
PSMB8 Antibody, Biotin conjugated
1-CSB-PA018886LD01HU
  • Ask for price
  • Ask for price
  • 100ul
  • 50ul
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
OACD05288-100UL - PSMB8 Antibody
OACD05288-100UL 100ul
EUR 199
OACD05289-100UL - PSMB8 Antibody
OACD05289-100UL 100ul
EUR 189
OACD05290-100UL - PSMB8 Antibody
OACD05290-100UL 100ul
EUR 189
OAAN02231-100UL - PSMB8 Antibody
OAAN02231-100UL 100ul
EUR 249
OAAN02231-200UL - PSMB8 Antibody
OAAN02231-200UL 200ul
EUR 389
OAEB02189-100UG - PSMB8 Antibody
OAEB02189-100UG 100ug
EUR 349
OAGA01026-100UL - PSMB8 antibody
OAGA01026-100UL 100ul
EUR 369
PSMB8 Antibody, Biotin Conjugated
MBS7113269-005mL 0.05mL
EUR 190
PSMB8 Antibody, Biotin Conjugated
MBS7113269-01mL 0.1mL
EUR 270
PSMB8 Antibody, Biotin Conjugated
MBS7113269-5x01mL 5x0.1mL
EUR 1205
PSMB8 Rabbit Polyclonal Antibody
55187 100ul
EUR 439
PSMB8 Rabbit Polyclonal Antibody
E10G05021 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody
PSMB8 Rabbit Polyclonal Antibody
E10G11908 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody
PSMB8 Rabbit Polyclonal Antibody
MBS9466218-005mL 0.05mL
EUR 300
PSMB8 Rabbit Polyclonal Antibody
MBS9466218-01mL 0.1mL
EUR 390
PSMB8 Rabbit Polyclonal Antibody
MBS9466218-5x01mL 5x0.1mL
EUR 1610
PSMB8 Antibody - N-terminal region
MBS3208052-01mL 0.1mL
EUR 455